{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,29]],"date-time":"2026-10-29T03:56:51Z","timestamp":1793246211179,"version":"4.5.0"},"reference-count":32,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2021,9,2]],"date-time":"2021-09-02T00:00:00Z","timestamp":1630540800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000268","name":"Biotechnology and Biological Sciences Research Council","doi-asserted-by":"publisher","award":["BB\/R00708X\/1"],"award-info":[{"award-number":["BB\/R00708X\/1"]}],"id":[{"id":"10.13039\/501100000268","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000268","name":"Biotechnology and Biological Sciences Research Council","doi-asserted-by":"publisher","award":["BB\/R007985\/1"],"award-info":[{"award-number":["BB\/R007985\/1"]}],"id":[{"id":"10.13039\/501100000268","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells"],"abstract":"<jats:p>Approximately one million in vitro produced (IVP) cattle embryos are transferred worldwide each year as a way to improve the rates of genetic gain. The most advanced programmes also apply genomic selection at the embryonic stage by SNP genotyping and the calculation of genomic estimated breeding values (GEBVs). However, a high proportion of cattle embryos fail to establish a pregnancy. Here, we demonstrate that further interrogation of the SNP data collected for GEBVs can effectively remove aneuploid embryos from the pool, improving live births per embryo transfer (ET). Using three preimplantation genetic testing for aneuploidy (PGT-A) approaches, we assessed 1713 cattle blastocysts in a blind, retrospective analysis. Our findings indicate aneuploid embryos have a 5.8% chance of establishing a pregnancy and a 5.0% chance of given rise to a live birth. This compares to 59.6% and 46.7% for euploid embryos (p &lt; 0.0001). PGT-A improved overall pregnancy and live birth rates by 7.5% and 5.8%, respectively (p &lt; 0.0001). More detailed analyses revealed donor, chromosome, stage, grade, and sex-specific rates of error. Notably, we discovered a significantly higher incidence of aneuploidy in XY embryos and, as in humans, detected a preponderance of maternal meiosis I errors. Our data strongly support the use of PGT-A in cattle IVP programmes.<\/jats:p>","DOI":"10.3390\/cells10092284","type":"journal-article","created":{"date-parts":[[2021,9,2]],"date-time":"2021-09-02T10:02:11Z","timestamp":1630576931000},"page":"2284","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":39,"title":["Preimplantation Genetic Testing for Aneuploidy Improves Live Birth Rates with In Vitro Produced Bovine Embryos: A Blind Retrospective Study"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5496-2470","authenticated-orcid":false,"given":"Giuseppe","family":"Silvestri","sequence":"first","affiliation":[{"name":"School of Biosciences, University of Kent, Canterbury CT2 7NH, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1575-7420","authenticated-orcid":false,"given":"Carla","family":"Canedo-Ribeiro","sequence":"additional","affiliation":[{"name":"School of Biosciences, University of Kent, Canterbury CT2 7NH, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6234-4058","authenticated-orcid":false,"given":"Mar\u00eda","family":"Serrano-Albal","sequence":"additional","affiliation":[{"name":"School of Biosciences, University of Kent, Canterbury CT2 7NH, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Remi","family":"Labrecque","sequence":"additional","affiliation":[{"name":"L\u2019Alliance Boviteq Inc., Saint-Hyacinthe, QC J2T 5H1, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrick","family":"Blondin","sequence":"additional","affiliation":[{"name":"L\u2019Alliance Boviteq Inc., Saint-Hyacinthe, QC J2T 5H1, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steven G.","family":"Larmer","sequence":"additional","affiliation":[{"name":"L\u2019Alliance Boviteq Inc., Saint-Hyacinthe, QC J2T 5H1, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriele","family":"Marras","sequence":"additional","affiliation":[{"name":"L\u2019Alliance Boviteq Inc., Saint-Hyacinthe, QC J2T 5H1, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Desmond A.R.","family":"Tutt","sequence":"additional","affiliation":[{"name":"School of Biosciences, University of Nottingham, Nottingham LE12 5RD, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alan H.","family":"Handyside","sequence":"additional","affiliation":[{"name":"School of Biosciences, University of Kent, Canterbury CT2 7NH, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9170-5767","authenticated-orcid":false,"given":"Marta","family":"Farr\u00e9","sequence":"additional","affiliation":[{"name":"School of Biosciences, University of Kent, Canterbury CT2 7NH, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6375-215X","authenticated-orcid":false,"given":"Kevin D.","family":"Sinclair","sequence":"additional","affiliation":[{"name":"School of Biosciences, University of Nottingham, Nottingham LE12 5RD, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7595-3226","authenticated-orcid":false,"given":"Darren K.","family":"Griffin","sequence":"additional","affiliation":[{"name":"School of Biosciences, University of Kent, Canterbury CT2 7NH, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,9,2]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"991","DOI":"10.1017\/S1751731119002775","article-title":"Recent Advances in Bovine in Vitro Embryo Production: Reproductive Biotechnology History and Methods","volume":"14","author":"Kjelland","year":"2020","journal-title":"Animal"},{"key":"ref_2","first-page":"81","article-title":"Embryo Biopsies for Genomic Selection","volume":"Volume 2","author":"Niemann","year":"2018","journal-title":"Animal Biotechnology"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1262\/jrd.2019-009","article-title":"Potential of Preimplantation Genomic Selection for Carcass Traits in Japanese Black Cattle","volume":"65","author":"Fujii","year":"2019","journal-title":"J. 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Genet."}],"container-title":["Cells"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2073-4409\/10\/9\/2284\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T06:54:41Z","timestamp":1760165681000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2073-4409\/10\/9\/2284"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,9,2]]},"references-count":32,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2021,9]]}},"alternative-id":["cells10092284"],"URL":"https:\/\/doi.org\/10.3390\/cells10092284","relation":{},"ISSN":["2073-4409"],"issn-type":[{"value":"2073-4409","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,9,2]]}}}