{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T06:44:00Z","timestamp":1792565040234,"version":"4.5.0"},"reference-count":91,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2021,9,5]],"date-time":"2021-09-05T00:00:00Z","timestamp":1630800000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003710","name":"Korea Health Industry Development Institute","doi-asserted-by":"publisher","award":["HI20C0493"],"award-info":[{"award-number":["HI20C0493"]}],"id":[{"id":"10.13039\/501100003710","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells"],"abstract":"<jats:p>Current treatment options for patients infected with hepatitis B virus (HBV) are suboptimal, because the approved drugs rarely induce cure due to the persistence of the viral DNA genome in the nucleus of infected hepatocytes, and are associated with either severe side effects (pegylated interferon-alpha) or require life-long administration (nucleos(t)ide analogs). We report here the evaluation of the safety and therapeutic efficacy of a novel, humanized antibody (hzVSF) in the woodchuck model of HBV infection. hzVSF has been shown to act as a viral entry inhibitor, most likely by suppressing vimentin-mediated endocytosis of virions. Targeting the increased vimentin expression on liver cells by hzVSF after infection with HBV or woodchuck hepatitis virus (WHV) was demonstrated initially. Thereafter, hzVSF safety was assessed in eight woodchucks na\u00efve for WHV infection. Antiviral efficacy of hzVSF was evaluated subsequently in 24 chronic WHV carrier woodchucks by monotreatment with three ascending doses and in combination with tenofovir alafenamide fumarate (TAF). Consistent with the proposed blocking of WHV reinfection, intravenous hzVSF administration for 12 weeks resulted in a modest but transient reduction of viral replication and associated liver inflammation. In combination with oral TAF dosing, the antiviral effect of hzVSF was enhanced and sustained in half of the woodchucks with an antibody response to viral proteins. Thus, hzVSF safely but modestly alters chronic WHV infection in woodchucks; however, as a combination partner to TAF, its antiviral efficacy is markedly increased. The results of this preclinical study support future evaluation of this novel anti-HBV drug in patients.<\/jats:p>","DOI":"10.3390\/cells10092321","type":"journal-article","created":{"date-parts":[[2021,9,6]],"date-time":"2021-09-06T10:59:12Z","timestamp":1630925952000},"page":"2321","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Combination Treatment with the Vimentin-Targeting Antibody hzVSF and Tenofovir Suppresses Woodchuck Hepatitis Virus Infection in Woodchucks"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1116-6500","authenticated-orcid":false,"given":"Kyle E.","family":"Korolowicz","sequence":"first","affiliation":[{"name":"Department of Microbiology & Immunology, Georgetown University Medical Center, Washington, DC 20057, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1285-0374","authenticated-orcid":false,"given":"Manasa","family":"Suresh","sequence":"additional","affiliation":[{"name":"Department of Microbiology & Immunology, Georgetown University Medical Center, Washington, DC 20057, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bin","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Microbiology & Immunology, Georgetown University Medical Center, Washington, DC 20057, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xu","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Microbiology & Immunology, Georgetown University Medical Center, Washington, DC 20057, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Changsuek","family":"Yon","sequence":"additional","affiliation":[{"name":"Department of Microbiology & Immunology, Georgetown University Medical Center, Washington, DC 20057, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bhaskar V.","family":"Kallakury","sequence":"additional","affiliation":[{"name":"Department of Pathology, Georgetown University Medical Center, Washington, DC 20057, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kyoung-pil","family":"Lee","sequence":"additional","affiliation":[{"name":"ImmuneMed, Inc., Chuncheon BioTown, Soyanggang ro 32, Chuncheon-si 24232, Gangwon-do, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sungman","family":"Park","sequence":"additional","affiliation":[{"name":"ImmuneMed, Inc., Chuncheon BioTown, Soyanggang ro 32, Chuncheon-si 24232, Gangwon-do, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoon-Won","family":"Kim","sequence":"additional","affiliation":[{"name":"ImmuneMed, Inc., Chuncheon BioTown, Soyanggang ro 32, Chuncheon-si 24232, Gangwon-do, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6873-4084","authenticated-orcid":false,"given":"Stephan","family":"Menne","sequence":"additional","affiliation":[{"name":"Department of Microbiology & Immunology, Georgetown University Medical Center, Washington, DC 20057, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,9,5]]},"reference":[{"key":"ref_1","unstructured":"World Health Organiziation (2021). Hepatitis B, WHO."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"401","DOI":"10.1016\/j.cld.2019.04.002","article-title":"Understanding the Natural History of Hepatitis B Virus Infection and the New Definitions of Cure and the Endpoints of Clinical Trials","volume":"23","author":"Likhitsup","year":"2019","journal-title":"Clin. Liver Dis."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"827","DOI":"10.1038\/s41573-019-0037-0","article-title":"Therapeutic strategies for hepatitis B virus infection: Towards a cure","volume":"18","author":"Fanning","year":"2019","journal-title":"Nat. Rev. Drug Discov."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1111\/liv.13309","article-title":"Treatment of chronic hepatitis B infection-2017","volume":"37","author":"Chen","year":"2017","journal-title":"Liver Int."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"a021386","DOI":"10.1101\/cshperspect.a021386","article-title":"Hepadnavirus Genome Replication and Persistence","volume":"5","author":"Hu","year":"2015","journal-title":"Cold Spring Harb. Perspect. Med."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1759","DOI":"10.1002\/hep.21896","article-title":"Hepatitis B virus infection initiates with a large surface protein-dependent binding to heparan sulfate proteoglycans","volume":"46","author":"Schulze","year":"2007","journal-title":"Hepatology"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"985","DOI":"10.1002\/hep.26125","article-title":"A conformational heparan sulfate binding site essential to infectivity overlaps with the conserved hepatitis B virus a-determinant","volume":"57","author":"Sureau","year":"2013","journal-title":"Hepatology"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"322","DOI":"10.1039\/C8BM01134F","article-title":"A hepatitis B virus-derived human hepatic cell-specific heparin-binding peptide: Identification and application to a drug delivery system","volume":"7","author":"Liu","year":"2018","journal-title":"Biomater. Sci."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1070","DOI":"10.1053\/j.gastro.2013.12.024","article-title":"Hepatitis B and D viruses exploit sodium taurocholate co-transporting polypeptide for species-specific entry into hepatocytes","volume":"146","author":"Ni","year":"2014","journal-title":"Gastroenterology"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"e00049","DOI":"10.7554\/eLife.00049","article-title":"Sodium taurocholate cotransporting polypeptide is a functional receptor for human hepatitis B and D virus","volume":"1","author":"Yan","year":"2012","journal-title":"eLife"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"8487","DOI":"10.1073\/pnas.1811064116","article-title":"Epidermal growth factor receptor is a host-entry cofactor triggering hepatitis B virus internalization","volume":"116","author":"Iwamoto","year":"2019","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"9443","DOI":"10.1128\/JVI.00873-12","article-title":"Entry of hepatitis B virus into immortalized human primary hepatocytes by clathrin-dependent endocytosis","volume":"86","author":"Huang","year":"2012","journal-title":"J. Virol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"800","DOI":"10.1016\/S0021-9258(17)49936-4","article-title":"The machinery for endocytosis of epidermal growth factor receptor coordinates the transport of incoming hepatitis B virus to the endosomal network","volume":"295","author":"Iwamoto","year":"2020","journal-title":"J. Biol. Chem."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"e13205","DOI":"10.1111\/cmi.13205","article-title":"Hepatitis B virus entry into HepG2-NTCP cells requires clathrin-mediated endocytosis","volume":"22","author":"Herrscher","year":"2020","journal-title":"Cell. Microbiol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"6415","DOI":"10.1128\/JVI.00393-13","article-title":"Regulation of hepatitis B virus infection by Rab5, Rab7, and the endolysosomal compartment","volume":"87","author":"Macovei","year":"2013","journal-title":"J. Virol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"16563","DOI":"10.1074\/jbc.M601418200","article-title":"Clathrin-mediated endocytosis and lysosomal cleavage of hepatitis B virus capsid-like core particles","volume":"281","author":"Cooper","year":"2006","journal-title":"J. Biol. Chem."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"3481","DOI":"10.1038\/emboj.2011.286","article-title":"Endosome maturation","volume":"30","author":"Huotari","year":"2011","journal-title":"EMBO J."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1159\/000360948","article-title":"Inhibitors of hepatitis B virus attachment and entry","volume":"57","author":"Lempp","year":"2014","journal-title":"Intervirology"},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Herrscher, C., Roingeard, P., and Blanchard, E. (2020). Hepatitis B Virus Entry into Cells. Cells, 9.","DOI":"10.3390\/cells9061486"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1989","DOI":"10.1128\/JVI.01902-09","article-title":"Fine mapping of pre-S sequence requirements for hepatitis B virus large envelope protein-mediated receptor interaction","volume":"84","author":"Schulze","year":"2010","journal-title":"J. Virol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"S32","DOI":"10.1016\/j.jhep.2016.02.011","article-title":"Entry of hepatitis B and hepatitis D virus into hepatocytes: Basic insights and clinical implications","volume":"64","author":"Li","year":"2016","journal-title":"J. Hepatol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"490","DOI":"10.1016\/j.jhep.2016.04.016","article-title":"Treatment of chronic hepatitis D with the entry inhibitor myrcludex B: First results of a phase Ib\/IIa study","volume":"65","author":"Bogomolov","year":"2016","journal-title":"J. Hepatol."},{"key":"ref_23","unstructured":"Kim, Y.W., Kim, Y.J., Hong, H.J., Park, S.J., Kim, M.W., and Park, S. (2016). An Antibody or Peptide Specifically Binding to Peptide Derived from Vimentin. (10201600726972016), Korea Patent."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"9553","DOI":"10.1128\/JVI.72.12.9553-9560.1998","article-title":"Interaction of Theiler\u2019s virus with intermediate filaments of infected cells","volume":"72","author":"Nedellec","year":"1998","journal-title":"J. Virol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"11766","DOI":"10.1128\/JVI.79.18.11766-11775.2005","article-title":"Vimentin rearrangement during African swine fever virus infection involves retrograde transport along microtubules and phosphorylation of vimentin by calcium calmodulin kinase II","volume":"79","author":"Stefanovic","year":"2005","journal-title":"J. Virol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1839","DOI":"10.1128\/JVI.76.4.1839-1855.2002","article-title":"Endoplasmic reticulum-Golgi intermediate compartment membranes and vimentin filaments participate in vaccinia virus assembly","volume":"76","author":"Risco","year":"2002","journal-title":"J. Virol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"e01393-19","DOI":"10.1128\/JVI.01393-19","article-title":"Human Enterovirus Group B Viruses Rely on Vimentin Dynamics for Efficient Processing of Viral Nonstructural Proteins","volume":"94","author":"Turkki","year":"2020","journal-title":"J. Virol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"9198","DOI":"10.1128\/MCB.24.20.9198-9206.2004","article-title":"The endothelial cell-specific antibody PAL-E identifies a secreted form of vimentin in the blood vasculature","volume":"24","author":"Xu","year":"2004","journal-title":"Mol. Cell. Biol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"156","DOI":"10.1038\/ncb1355","article-title":"Vimentin function in lymphocyte adhesion and transcellular migration","volume":"8","author":"Nieminen","year":"2006","journal-title":"Nat. Cell Biol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"6574","DOI":"10.1038\/ncomms7574","article-title":"Vimentin regulates activation of the NLRP3 inflammasome","volume":"6","author":"Rogel","year":"2015","journal-title":"Nat. Commun."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"2349","DOI":"10.1158\/0008-5472.CAN-14-2842","article-title":"Vimentin-ERK Signaling Uncouples Slug Gene Regulatory Function","volume":"75","author":"Virtakoivu","year":"2015","journal-title":"Cancer Res."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"e2000078","DOI":"10.1002\/bies.202000078","article-title":"Mechanical and Non-Mechanical Functions of Filamentous and Non-Filamentous Vimentin","volume":"42","author":"Patteson","year":"2020","journal-title":"Bioessays"},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Danielsson, F., Peterson, M.K., Caldeira Araujo, H., Lautenschlager, F., and Gad, A.K.B. (2018). Vimentin Diversity in Health and Disease. Cells, 7.","DOI":"10.3390\/cells7100147"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"7015","DOI":"10.1128\/JVI.00398-09","article-title":"Onset of human cytomegalovirus replication in fibroblasts requires the presence of an intact vimentin cytoskeleton","volume":"83","author":"Miller","year":"2009","journal-title":"J. Virol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"e07586","DOI":"10.1016\/j.heliyon.2021.e07586","article-title":"Humanized virus-suppressing factor inhibits hepatitis B virus infection by targeting viral cell entry","volume":"7","author":"Miyakawa","year":"2021","journal-title":"Heliyon"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"E4574","DOI":"10.1073\/pnas.1703057114","article-title":"Selective regulation of Notch ligands during angiogenesis is mediated by vimentin","volume":"114","author":"Antfolk","year":"2017","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"2371","DOI":"10.1002\/jmv.26063","article-title":"Compassionate use of hzVSF-v13 in two patients with severe COVID-19","volume":"92","author":"Kang","year":"2020","journal-title":"J. Med. Virol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"104","DOI":"10.3748\/wjg.v13.i1.104","article-title":"The woodchuck as an animal model for pathogenesis and therapy of chronic hepatitis B virus infection","volume":"13","author":"Menne","year":"2007","journal-title":"World J. Gastroenterol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"190","DOI":"10.1002\/hep.510310128","article-title":"Effects of age and viral determinants on chronicity as an outcome of experimental woodchuck hepatitis virus infection","volume":"31","author":"Cote","year":"2000","journal-title":"Hepatology"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"S283","DOI":"10.1053\/j.gastro.2004.09.043","article-title":"Hepatocellular carcinoma in the woodchuck model of hepatitis B virus infection","volume":"127","author":"Tennant","year":"2004","journal-title":"Gastroenterology"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"a021451","DOI":"10.1101\/cshperspect.a021451","article-title":"The Woodchuck, a Nonprimate Model for Immunopathogenesis and Therapeutic Immunomodulation in Chronic Hepatitis B Virus Infection","volume":"5","author":"Roggendorf","year":"2015","journal-title":"Cold Spring Harb. Perspect. Med."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1007\/s00430-014-0379-5","article-title":"Therapeutic vaccination and immunomodulation in the treatment of chronic hepatitis B: Preclinical studies in the woodchuck","volume":"204","author":"Kosinska","year":"2015","journal-title":"Med. Microbiol. Immunol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"853","DOI":"10.3389\/fimmu.2020.00853","article-title":"Diverse Virus and Host-Dependent Mechanisms Influence the Systemic and Intrahepatic Immune Responses in the Woodchuck Model of Hepatitis B","volume":"11","author":"Michalak","year":"2020","journal-title":"Front. Immunol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/j.coviro.2018.01.008","article-title":"Modulators of innate immunity as novel therapeutics for treatment of chronic hepatitis B","volume":"30","author":"Suslov","year":"2018","journal-title":"Curr. Opin. Virol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"509","DOI":"10.4251\/wjgo.v13.i6.509","article-title":"Application of the woodchuck animal model for the treatment of hepatitis B virus-induced liver cancer","volume":"13","author":"Suresh","year":"2021","journal-title":"World J. Gastrointest. Oncol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"1165","DOI":"10.1053\/he.2000.5982","article-title":"Treatment of chronic woodchuck hepatitis virus infection in the Eastern woodchuck (Marmota monax) with nucleoside analogues is predictive of therapy for chronic hepatitis B virus infection in humans","volume":"31","author":"Korba","year":"2000","journal-title":"Hepatology"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"1236","DOI":"10.1086\/324003","article-title":"Long-term entecavir treatment results in sustained antiviral efficacy and prolonged life span in the woodchuck model of chronic hepatitis infection","volume":"184","author":"Colonno","year":"2001","journal-title":"J. Infect. Dis."},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Korolowicz, K.E., Iyer, R.P., Czerwinski, S., Suresh, M., Yang, J., Padmanabhan, S., Sheri, A., Pandey, R.K., Skell, J., and Marquis, J.K. (2016). Antiviral Efficacy and Host Innate Immunity Associated with SB 9200 Treatment in the Woodchuck Model of Chronic Hepatitis B. PLoS ONE, 11.","DOI":"10.1371\/journal.pone.0161313"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1002\/hep.31255","article-title":"Toll-Like Receptor 8 Agonist GS-9688 Induces Sustained Efficacy in the Woodchuck Model of Chronic Hepatitis B","volume":"73","author":"Daffis","year":"2020","journal-title":"Hepatology"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.virol.2017.02.006","article-title":"Woodchuck sodium taurocholate cotransporting polypeptide supports low-level hepatitis B and D virus entry","volume":"505","author":"Fu","year":"2017","journal-title":"Virology"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"1837","DOI":"10.1099\/0022-1317-77-8-1837","article-title":"Protease-activated lymphoid cell and hepatocyte recognition site in the preS1 domain of the large woodchuck hepatitis virus envelope protein","volume":"77","author":"Jin","year":"1996","journal-title":"J. Gen. Virol."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"7276","DOI":"10.1128\/JVI.00576-08","article-title":"Primary human hepatocytes are susceptible to infection by hepatitis delta virus assembled with envelope proteins of woodchuck hepatitis virus","volume":"82","author":"Gudima","year":"2008","journal-title":"J. Virol."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"533","DOI":"10.1016\/j.jhep.2014.10.035","article-title":"Twenty-eight day safety, antiviral activity, and pharmacokinetics of tenofovir alafenamide for treatment of chronic hepatitis B infection","volume":"62","author":"Agarwal","year":"2015","journal-title":"J. Hepatol."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"3617","DOI":"10.1128\/AAC.00654-08","article-title":"Antiviral effects of lamivudine, emtricitabine, adefovir dipivoxil, and tenofovir disoproxil fumarate administered orally alone and in combination to woodchucks with chronic woodchuck hepatitis virus infection","volume":"52","author":"Menne","year":"2008","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_55","first-page":"376","article-title":"Laboratory assessment of hepatic injury in the woodchuck (Marmota monax)","volume":"35","author":"Hornbuckle","year":"1985","journal-title":"Lab. Anim. Sci."},{"key":"ref_56","doi-asserted-by":"crossref","unstructured":"Korolowicz, K.E., Suresh, M., Li, B., Huang, X., Yon, C., Leng, X., Kallakury, B.V., Tucker, R.D., and Menne, S. (2021). Treatment with the Immunomodulator AIC649 in Combination with Entecavir Produces Antiviral Efficacy in the Woodchuck Model of Chronic Hepatitis B. Viruses, 13.","DOI":"10.3390\/v13040648"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1089\/vim.1993.6.161","article-title":"New enzyme immunoassays for the serologic detection of woodchuck hepatitis virus infection","volume":"6","author":"Cote","year":"1993","journal-title":"Viral Immunol."},{"key":"ref_58","doi-asserted-by":"crossref","unstructured":"Bellezza, C.A., Concannon, P.W., Hornbuckle, W.E., Roth, L., and Tennant, B.C. (2002). Woodchucks as laboratory animals. Laboratory Animal Medicine, Elsevier Science. [2nd ed.].","DOI":"10.1016\/B978-012263951-7\/50011-9"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"254","DOI":"10.1053\/jhep.2001.20899","article-title":"Antiviral activity of clevudine [L-FMAU, (1-(2-fluoro-5-methyl-beta, L-arabinofuranosyl) uracil)] against woodchuck hepatitis virus replication and gene expression in chronically infected woodchucks (Marmota monax)","volume":"33","author":"Peek","year":"2001","journal-title":"Hepatology"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1002\/hep.510280124","article-title":"Antiviral activity and toxicity of fialuridine in the woodchuck model of hepatitis B virus infection","volume":"28","author":"Tennant","year":"1998","journal-title":"Hepatology"},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"354","DOI":"10.1038\/nm1368","article-title":"Viral nanoparticles as tools for intravital vascular imaging","volume":"12","author":"Lewis","year":"2006","journal-title":"Nat. Med."},{"key":"ref_62","doi-asserted-by":"crossref","unstructured":"Koudelka, K.J., Destito, G., Plummer, E.M., Trauger, S.A., Siuzdak, G., and Manchester, M. (2009). Endothelial targeting of cowpea mosaic virus (CPMV) via surface vimentin. PLoS Pathog., 5.","DOI":"10.1371\/journal.ppat.1000417"},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1186\/s12929-016-0234-7","article-title":"Surface vimentin is critical for the cell entry of SARS-CoV","volume":"23","author":"Yu","year":"2016","journal-title":"J. Biomed. Sci."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"404","DOI":"10.1016\/j.virusres.2011.06.001","article-title":"Japanese encephalitis virus interacts with vimentin to facilitate its entry into porcine kidney cell line","volume":"160","author":"Das","year":"2011","journal-title":"Virus Res."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1007\/s13365-019-00800-8","article-title":"Enterovirus A71 capsid protein VP1 increases blood-brain barrier permeability and virus receptor vimentin on the brain endothelial cells","volume":"26","author":"Wang","year":"2020","journal-title":"J. Neurovirol."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"689","DOI":"10.1128\/JVI.80.2.689-696.2006","article-title":"Defining the cellular target(s) of porcine reproductive and respiratory syndrome virus blocking monoclonal antibody 7G10","volume":"80","author":"Kim","year":"2006","journal-title":"J. Virol."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"1358","DOI":"10.1111\/j.1462-5822.2011.01624.x","article-title":"Vimentin binding is critical for infection by the virulent strain of Japanese encephalitis virus","volume":"13","author":"Liang","year":"2011","journal-title":"Cell. Microbiol."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"5816","DOI":"10.1128\/JVI.03826-13","article-title":"Cell surface vimentin is an attachment receptor for enterovirus 71","volume":"88","author":"Du","year":"2014","journal-title":"J. Virol."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"38372","DOI":"10.1038\/srep38372","article-title":"Superficial vimentin mediates DENV-2 infection of vascular endothelial cells","volume":"6","author":"Yang","year":"2016","journal-title":"Sci. Rep."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1186\/s12985-020-01351-9","article-title":"The molecular evolutionary characteristics of new isolated H9N2 AIV from East China and the function of vimentin on virus replication in MDCK cells","volume":"17","author":"Yu","year":"2020","journal-title":"Virol. J."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"198014","DOI":"10.1016\/j.virusres.2020.198014","article-title":"Role of cell surface vimentin in Chandipura virus replication in Neuro-2a cells","volume":"285","author":"Kavathekar","year":"2020","journal-title":"Virus Res."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"861","DOI":"10.1016\/j.jhep.2012.12.008","article-title":"The entry inhibitor Myrcludex-B efficiently blocks intrahepatic virus spreading in humanized mice previously infected with hepatitis B virus","volume":"58","author":"Volz","year":"2013","journal-title":"J. Hepatol."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"104999","DOI":"10.1016\/j.antiviral.2020.104999","article-title":"Ponesimod suppresses hepatitis B virus infection by inhibiting endosome maturation","volume":"186","author":"Fauzyah","year":"2021","journal-title":"Antivir. Res."},{"key":"ref_74","first-page":"20","article-title":"Inhibitory effect of silibinin on hepatitis B virus entry","volume":"14","author":"Umetsu","year":"2018","journal-title":"Biochem. Biophys. Rep."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1016\/j.antiviral.2014.09.009","article-title":"(\u2212)-Epigallocatechin-3-gallate inhibits entry of hepatitis B virus into hepatocytes","volume":"111","author":"Huang","year":"2014","journal-title":"Antivir. Res."},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1016\/S2468-1253(16)30024-3","article-title":"Tenofovir alafenamide versus tenofovir disoproxil fumarate for the treatment of HBeAg-positive chronic hepatitis B virus infection: A randomised, double-blind, phase 3, non-inferiority trial","volume":"1","author":"Chan","year":"2016","journal-title":"Lancet Gastroenterol. Hepatol."},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"1317","DOI":"10.1111\/j.1365-2036.2012.05093.x","article-title":"Renal tubular dysfunction during long-term adefovir or tenofovir therapy in chronic hepatitis B","volume":"35","author":"Gara","year":"2012","journal-title":"Aliment. Pharmacol. Ther."},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"1296","DOI":"10.1002\/hep4.1397","article-title":"Liver-Targeted Toll-Like Receptor 7 Agonist Combined With Entecavir Promotes a Functional Cure in the Woodchuck Model of Hepatitis B Virus","volume":"3","author":"Korolowizc","year":"2019","journal-title":"Hepatol. Commun."},{"key":"ref_79","doi-asserted-by":"crossref","unstructured":"Liu, J., Zhang, E., Ma, Z., Wu, W., Kosinska, A., Zhang, X., Moller, I., Seiz, P., Glebe, D., and Wang, B. (2014). Enhancing virus-specific immunity in vivo by combining therapeutic vaccination and PD-L1 blockade in chronic hepadnaviral infection. PLoS Pathog., 10.","DOI":"10.1371\/journal.ppat.1003856"},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"2720","DOI":"10.1128\/AAC.49.7.2720-2728.2005","article-title":"Antiviral effect of oral administration of tenofovir disoproxil fumarate in woodchucks with chronic woodchuck hepatitis virus infection","volume":"49","author":"Menne","year":"2005","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1016\/j.antiviral.2015.11.001","article-title":"Combination therapy including CpG oligodeoxynucleotides and entecavir induces early viral response and enhanced inhibition of viral replication in a woodchuck model of chronic hepadnaviral infection","volume":"125","author":"Meng","year":"2016","journal-title":"Antivir. Res."},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"4588","DOI":"10.1172\/JCI121960","article-title":"Circulating and intrahepatic antiviral B cells are defective in hepatitis B","volume":"128","author":"Burton","year":"2018","journal-title":"J. Clin. Investig."},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"4573","DOI":"10.1172\/JCI121957","article-title":"PD-1 blockade partially recovers dysfunctional virus-specific B cells in chronic hepatitis B infection","volume":"128","author":"Salimzadeh","year":"2018","journal-title":"J. Clin. Investig."},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1038\/cmi.2015.25","article-title":"Reversal of B-cell hyperactivation and functional impairment is associated with HBsAg seroconversion in chronic hepatitis B patients","volume":"12","author":"Xu","year":"2015","journal-title":"Cell. Mol. Immunol."},{"key":"ref_85","doi-asserted-by":"crossref","first-page":"1237","DOI":"10.1016\/j.jhep.2014.12.026","article-title":"Sustained efficacy and seroconversion with the Toll-like receptor 7 agonist GS-9620 in the Woodchuck model of chronic hepatitis B","volume":"62","author":"Menne","year":"2015","journal-title":"J. Hepatol."},{"key":"ref_86","doi-asserted-by":"crossref","first-page":"5305","DOI":"10.1128\/JVI.76.11.5305-5314.2002","article-title":"Immunization with surface antigen vaccine alone and after treatment with 1-(2-fluoro-5-methyl-beta-L-arabinofuranosyl)-uracil (L-FMAU) breaks humoral and cell-mediated immune tolerance in chronic woodchuck hepatitis virus infection","volume":"76","author":"Menne","year":"2002","journal-title":"J. Virol."},{"key":"ref_87","doi-asserted-by":"crossref","first-page":"1760","DOI":"10.1111\/liv.13716","article-title":"ALT flares during nucleotide analogue therapy are associated with HBsAg loss in genotype A HBeAg-positive chronic hepatitis B","volume":"38","author":"Wong","year":"2018","journal-title":"Liver Int."},{"key":"ref_88","first-page":"704","article-title":"Effect of vimentin on activation of NLRP3 inflammasome in the brain of mice with EV71 infection","volume":"38","author":"Xiao","year":"2018","journal-title":"Nan Fang Yi Ke Da Xue Xue Bao"},{"key":"ref_89","doi-asserted-by":"crossref","first-page":"1045","DOI":"10.1038\/srep01045","article-title":"Murine colitis is mediated by vimentin","volume":"3","author":"Legendre","year":"2013","journal-title":"Sci. Rep."},{"key":"ref_90","doi-asserted-by":"crossref","first-page":"731","DOI":"10.1046\/j.1523-1747.2001.01305.x","article-title":"Increased expression of a novel early activation surface membrane receptor in cutaneous T cell lymphoma cells","volume":"116","author":"Nikolova","year":"2001","journal-title":"J. Investig. Dermatol."},{"key":"ref_91","doi-asserted-by":"crossref","first-page":"652","DOI":"10.4049\/jimmunol.176.1.652","article-title":"SC5 mAb represents a unique tool for the detection of extracellular vimentin as a specific marker of Sezary cells","volume":"176","author":"Huet","year":"2006","journal-title":"J. Immunol."}],"container-title":["Cells"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2073-4409\/10\/9\/2321\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T06:56:42Z","timestamp":1760165802000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2073-4409\/10\/9\/2321"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,9,5]]},"references-count":91,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2021,9]]}},"alternative-id":["cells10092321"],"URL":"https:\/\/doi.org\/10.3390\/cells10092321","relation":{},"ISSN":["2073-4409"],"issn-type":[{"value":"2073-4409","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,9,5]]}}}