{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,6]],"date-time":"2026-09-06T00:47:39Z","timestamp":1788655659187,"version":"build-2803163510"},"reference-count":135,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2021,9,22]],"date-time":"2021-09-22T00:00:00Z","timestamp":1632268800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["417912216"],"award-info":[{"award-number":["417912216"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010275","name":"Deutsche Gesellschaft f\u00fcr Muskelkranke","doi-asserted-by":"publisher","award":["Spletter_2019"],"award-info":[{"award-number":["Spletter_2019"]}],"id":[{"id":"10.13039\/501100010275","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells"],"abstract":"<jats:p>The proper regulation of RNA processing is critical for muscle development and the fine-tuning of contractile ability among muscle fiber-types. RNA binding proteins (RBPs) regulate the diverse steps in RNA processing, including alternative splicing, which generates fiber-type specific isoforms of structural proteins that confer contractile sarcomeres with distinct biomechanical properties. Alternative splicing is disrupted in muscle diseases such as myotonic dystrophy and dilated cardiomyopathy and is altered after intense exercise as well as with aging. It is therefore important to understand splicing and RBP function, but currently, only a small fraction of the hundreds of annotated RBPs expressed in muscle have been characterized. Here, we demonstrate the utility of Drosophila as a genetic model system to investigate basic developmental mechanisms of RBP function in myogenesis. We find that RBPs exhibit dynamic temporal and fiber-type specific expression patterns in mRNA-Seq data and display muscle-specific phenotypes. We performed knockdown with 105 RNAi hairpins targeting 35 RBPs and report associated lethality, flight, myofiber and sarcomere defects, including flight muscle phenotypes for Doa, Rm62, mub, mbl, sbr, and clu. Knockdown phenotypes of spliceosome components, as highlighted by phenotypes for A-complex components SF1 and Hrb87F (hnRNPA1), revealed level- and temporal-dependent myofibril defects. We further show that splicing mediated by SF1 and Hrb87F is necessary for Z-disc stability and proper myofibril development, and strong knockdown of either gene results in impaired localization of kettin to the Z-disc. Our results expand the number of RBPs with a described phenotype in muscle and underscore the diversity in myofibril and transcriptomic phenotypes associated with splicing defects. Drosophila is thus a powerful model to gain disease-relevant insight into cellular and molecular phenotypes observed when expression levels of splicing factors, spliceosome components and splicing dynamics are altered.<\/jats:p>","DOI":"10.3390\/cells10102505","type":"journal-article","created":{"date-parts":[[2021,9,22]],"date-time":"2021-09-22T09:14:57Z","timestamp":1632302097000},"page":"2505","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["A Candidate RNAi Screen Reveals Diverse RNA-Binding Protein Phenotypes in Drosophila Flight Muscle"],"prefix":"10.3390","volume":"10","author":[{"given":"Shao-Yen","family":"Kao","sequence":"first","affiliation":[{"name":"Biomedical Center, Department of Physiological Chemistry, Ludwig-Maximilians-Universit\u00e4t M\u00fcnchen, Gro\u00dfhaderner Str. 9, 82152 Martinsried-Planegg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elena","family":"Nikonova","sequence":"additional","affiliation":[{"name":"Biomedical Center, Department of Physiological Chemistry, Ludwig-Maximilians-Universit\u00e4t M\u00fcnchen, Gro\u00dfhaderner Str. 9, 82152 Martinsried-Planegg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sabrina","family":"Chaabane","sequence":"additional","affiliation":[{"name":"Biomedical Center, Department of Physiological Chemistry, Ludwig-Maximilians-Universit\u00e4t M\u00fcnchen, Gro\u00dfhaderner Str. 9, 82152 Martinsried-Planegg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Albiona","family":"Sabani","sequence":"additional","affiliation":[{"name":"Department of Biology, University of Wisconsin at Madison, 1117 W. Johnson St., Madison, WI 53706, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandra","family":"Martitz","sequence":"additional","affiliation":[{"name":"Molecular Nutrition Medicine, Else Kr\u00f6ner-Fresenius Center, Technical University of Munich, 85354 Freising, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anja","family":"Wittner","sequence":"additional","affiliation":[{"name":"Biomedical Center, Department of Physiological Chemistry, Ludwig-Maximilians-Universit\u00e4t M\u00fcnchen, Gro\u00dfhaderner Str. 9, 82152 Martinsried-Planegg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jakob","family":"Heemken","sequence":"additional","affiliation":[{"name":"Biomedical Center, Department of Physiological Chemistry, Ludwig-Maximilians-Universit\u00e4t M\u00fcnchen, Gro\u00dfhaderner Str. 9, 82152 Martinsried-Planegg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tobias","family":"Straub","sequence":"additional","affiliation":[{"name":"Biomedical Center, Bioinformatics Core Facility, Ludwig-Maximilians-Universit\u00e4t M\u00fcnchen, Gro\u00dfhaderner Str. 9, 82152 Martinsried-Planegg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2068-3350","authenticated-orcid":false,"given":"Maria L.","family":"Spletter","sequence":"additional","affiliation":[{"name":"Biomedical Center, Department of Physiological Chemistry, Ludwig-Maximilians-Universit\u00e4t M\u00fcnchen, Gro\u00dfhaderner Str. 9, 82152 Martinsried-Planegg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,9,22]]},"reference":[{"key":"ref_1","unstructured":"Hill, J., and Olsen, E. (2012). Muscle, Academic Press. [1st ed.]."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"92","DOI":"10.1016\/j.mod.2016.11.003","article-title":"Mechanical Forces during Muscle Development","volume":"144","author":"Lemke","year":"2017","journal-title":"Mech. Dev."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1007\/164_2016_53","article-title":"Assembly and Maintenance of Myofibrils in Striated Muscle","volume":"235","author":"Sanger","year":"2017","journal-title":"Actin Cytoskelet."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s13395-018-0152-3","article-title":"Diversification of the Muscle Proteome through Alternative Splicing","volume":"8","author":"Nakka","year":"2018","journal-title":"Skelet. Muscle"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"276","DOI":"10.1242\/jeb.128017","article-title":"Muscle Structural Assembly and Functional Consequences","volume":"219","author":"Narici","year":"2016","journal-title":"J. Exp. Biol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1016\/j.semcdb.2020.02.003","article-title":"Contributions of Alternative Splicing to Muscle Type Development and Function","volume":"104","author":"Nikonova","year":"2020","journal-title":"Semin. Cell Dev. Biol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1447","DOI":"10.1152\/physrev.00031.2010","article-title":"Fiber Types in Mammalian Skeletal Muscles","volume":"91","author":"Schiaffino","year":"2011","journal-title":"Physiol. Rev."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1016\/0014-5793(96)00338-9","article-title":"A Molecular Map of Titin\/Connectin Elasticity Reveals Two Different Mechanisms Acting in Series","volume":"385","author":"Gautel","year":"1996","journal-title":"FEBS Lett."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1007\/978-1-4615-4267-4_17","article-title":"Mechanical Properties of Titin Isoforms","volume":"481","author":"Granzier","year":"2000","journal-title":"Adv. Exp. Med. Biol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"311","DOI":"10.1002\/wrna.1276","article-title":"Functional Roles of Alternative Splicing Factors in Human Disease","volume":"6","author":"Cieply","year":"2015","journal-title":"Wiley Interdiscip. Rev. RNA"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1016\/j.biocel.2019.02.008","article-title":"Conserved Functions of RNA-Binding Proteins in Muscle","volume":"110","author":"Nikonova","year":"2019","journal-title":"Int. J. Biochem. Cell Biol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1215","DOI":"10.1007\/s00439-017-1802-y","article-title":"Faulty RNA Splicing: Consequences and Therapeutic Opportunities in Brain and Muscle Disorders","volume":"136","author":"Pagliarini","year":"2017","journal-title":"Hum. Genet."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1038\/nrg.2015.3","article-title":"RNA Mis-Splicing in Disease","volume":"17","author":"Scotti","year":"2016","journal-title":"Nat. Rev. Genet."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1016\/j.lfs.2016.11.016","article-title":"Satellite Cell Activation Induced by Aerobic Muscle Adaptation in Response to Endurance Exercise in Humans and Rodents","volume":"170","author":"Abreu","year":"2017","journal-title":"Life Sci."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"6237","DOI":"10.3390\/molecules20046237","article-title":"Model Organisms in the Fight against Muscular Dystrophy: Lessons from Drosophila and Zebrafish","volume":"20","author":"Daczewska","year":"2015","journal-title":"Molecules"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"654","DOI":"10.1152\/japplphysiol.00076.2019","article-title":"RNA-Binding Proteins: The next Step in Translating Skeletal Muscle Adaptations?","volume":"127","author":"Hettinger","year":"2019","journal-title":"J. Appl. Physiol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"959","DOI":"10.1016\/j.molcel.2015.01.037","article-title":"RNA Regulation by Poly(ADP-Ribose) Polymerases","volume":"58","author":"Bock","year":"2015","journal-title":"Mol. Cell"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"711","DOI":"10.1038\/s41586-020-2077-3","article-title":"A Large-Scale Binding and Functional Map of Human RNA-Binding Proteins","volume":"583","author":"Freese","year":"2020","journal-title":"Nature"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"696","DOI":"10.1016\/j.molcel.2016.06.029","article-title":"Comprehensive Identification of RNA-Binding Domains in Human Cells","volume":"63","author":"Castello","year":"2016","journal-title":"Mol. Cell"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1038\/nrm.2017.130","article-title":"A Brave New World of RNA-Binding Proteins","volume":"19","author":"Hentze","year":"2018","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"606","DOI":"10.1016\/j.cell.2016.03.040","article-title":"Rbfox Proteins Regulate Splicing as Part of a Large Multiprotein Complex LASR","volume":"165","author":"Damianov","year":"2016","journal-title":"Cell"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1755","DOI":"10.1101\/gad.1422106","article-title":"RS Domains Contact Splicing Signalsand Promote Splicing by a Commonmechanism in Yeast through Humans","volume":"20","author":"Shen","year":"2006","journal-title":"Genes Dev."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"76","DOI":"10.15698\/cst2020.04.217","article-title":"Role of RNA Binding Proteins with Prion-like Domains in Muscle and Neuromuscular Diseases","volume":"4","author":"Picchiarelli","year":"2020","journal-title":"Cell Stress"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1771","DOI":"10.1101\/gr.192518.115","article-title":"Regulation of Alternative Splicing in Drosophila by 56 RNA Binding Proteins","volume":"25","author":"Brooks","year":"2015","journal-title":"Genome Res."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1360","DOI":"10.1101\/gr.220517.117","article-title":"Ancient Antagonism between CELF and RBFOX Families Tunes MRNA Splicing Outcomes","volume":"27","author":"Gazzara","year":"2017","journal-title":"Genome Res."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"858","DOI":"10.1101\/gr.184390.114","article-title":"Antagonistic Regulation of MRNA Expression and Splicing by CELF and MBNL Proteins","volume":"25","author":"Wang","year":"2015","journal-title":"Genome Res."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"508","DOI":"10.1038\/s41586-018-0665-2","article-title":"TDP-43 and RNA Form Amyloid-like Myo-Granules in Regenerating Muscle","volume":"563","author":"Vogler","year":"2018","journal-title":"Nature"},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Wood, A., Gurfinkel, Y., Polain, N., Lamont, W., and Lyn Rea, S. (2021). Molecular Mechanisms Underlying TDP-43 Pathology in Cellular and Animal Models of ALS and FTLD. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms22094705"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"710","DOI":"10.1016\/j.cell.2012.06.041","article-title":"Transcriptome-Wide Regulation of Pre-MRNA Splicing and MRNA Localization by Muscleblind Proteins","volume":"150","author":"Wang","year":"2012","journal-title":"Cell"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"2510","DOI":"10.1101\/gad.11.19.2510","article-title":"Translational Repressor Bruno Plays Multiple Roles in Development and Is Widely Conserved","volume":"11","author":"Webster","year":"1997","journal-title":"Genes Dev."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"20333","DOI":"10.1073\/pnas.0809045105","article-title":"A Postnatal Switch of CELF and MBNL Proteins Reprograms Alternative Splicing in the Developing Heart","volume":"105","author":"Kalsotra","year":"2008","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Klinck, R., Fourrier, A., Thibault, P., Toutant, J., Durand, M., Lapointe, E., Caillet-Boudin, M.-L., Sergeant, N., Gourdon, G., and Meola, G. (2014). RBFOX1 Cooperates with MBNL1 to Control Splicing in Muscle, Including Events Altered in Myotonic Dystrophy Type 1. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0107324"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"715","DOI":"10.1038\/nrg3052","article-title":"Functional Consequences of Developmentally Regulated Alternative Splicing","volume":"12","author":"Kalsotra","year":"2011","journal-title":"Nat. Rev. Genet."},{"key":"ref_34","first-page":"1","article-title":"Stk38 Modulates Rbm24 Protein Stability to Regulate Sarcomere Assembly in Cardiomyocytes","volume":"7","author":"Liu","year":"2017","journal-title":"Sci. Rep."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"75","DOI":"10.7150\/jgen.12863","article-title":"GLAD: An Online Database of Gene List Annotation for Drosophila","volume":"3","author":"Hu","year":"2015","journal-title":"J. Genom."},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"The Gene Ontology Consortium, Carbon, S., Douglass, E., Good, B.M., Unni, D.R., Harris, N.L., Mungall, C.J., Basu, S., Chisholm, R.L., and Dodson, R.J. (2021). The Gene Ontology Resource: Enriching a GOld Mine. Nucleic Acids Res., 49, D325\u2013D334.","DOI":"10.1093\/nar\/gkaa1113"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"368","DOI":"10.3389\/fneur.2018.00368","article-title":"Abnormalities in Skeletal Muscle Myogenesis, Growth, and Regeneration in Myotonic Dystrophy","volume":"9","author":"Ausems","year":"2018","journal-title":"Front. Neurol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1016\/j.pediatrneurol.2020.01.003","article-title":"Overturning the Paradigm of Spinal Muscular Atrophy as Just a Motor Neuron Disease","volume":"109","author":"Yeo","year":"2020","journal-title":"Pediatr. Neurol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"288","DOI":"10.1097\/HCO.0000000000000845","article-title":"Genetics of Dilated Cardiomyopathy","volume":"36","author":"Chen","year":"2021","journal-title":"Curr. Opin. Cardiol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"2467","DOI":"10.1016\/j.biocel.2013.08.008","article-title":"RNA Binding Proteins in the Regulation of Heart Development","volume":"45","author":"Ladd","year":"2013","journal-title":"Int. J. Biochem. Cell Biol."},{"key":"ref_41","unstructured":"Taylor, M.V. (2013). Comparison of Muscle Development in Drosophila and Vertebrates. Muscle Development in Drosophila, Springer."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1016\/j.semcdb.2016.09.012","article-title":"Beyond Mice: Emerging and Transdisciplinary Models for the Study of Early-Onset Myopathies","volume":"64","author":"Jagla","year":"2017","journal-title":"Semin. Cell Dev. Biol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1387\/ijdb.190333un","article-title":"Indirect Flight Muscles in Drosophila Melanogaster as a Tractable Model to Study Muscle Development and Disease","volume":"64","author":"Jawkar","year":"2020","journal-title":"Int. J. Dev. Biol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"e1","DOI":"10.1111\/j.1749-6632.2010.05432.x","article-title":"Flightless Flies: Drosophila Models of Neuromuscular Disease","volume":"1184","author":"Lloyd","year":"2010","journal-title":"Ann. N. Y. Acad. Sci."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1007\/978-1-4939-8897-6_20","article-title":"RNA Interference Screening for Genes Regulating Drosophila Muscle Morphogenesis","volume":"1889","author":"Schnorrer","year":"2019","journal-title":"Methods Mol. Biol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1007\/s10974-009-9166-0","article-title":"Transgenic Tools for Drosophila Muscle Research","volume":"29","author":"Orfanos","year":"2008","journal-title":"J. Muscle Res. Cell Motil."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1016\/j.ymeth.2014.02.037","article-title":"A Guide to Study Drosophila Muscle Biology","volume":"68","author":"Weitkunat","year":"2014","journal-title":"Methods"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1002\/wdev.180","article-title":"Morphogenesis of the Somatic Musculature in Drosophila Melanogaster","volume":"4","author":"Schulman","year":"2015","journal-title":"Wiley Interdiscip. Rev. Dev. Biol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1016\/j.semcdb.2017.11.017","article-title":"Drosophila Adult Muscle Development and Regeneration","volume":"72","author":"Gunage","year":"2017","journal-title":"Semin. Cell Dev. Biol."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"e03126","DOI":"10.7554\/eLife.03126","article-title":"Identification of a New Stem Cell Population That Generates Drosophila Flight Muscles","volume":"3","author":"Gunage","year":"2014","journal-title":"Elife"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1016\/bs.ctdb.2016.07.003","article-title":"Drosophila Melanogaster as a Model of Muscle Degeneration Disorders","volume":"121","author":"Kreipke","year":"2017","journal-title":"Curr. Top. Dev. Biol."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1016\/j.semcdb.2017.10.033","article-title":"Acting on Identity: Myoblast Fusion and the Formation of the Syncytial Muscle Fiber","volume":"72","author":"Deng","year":"2017","journal-title":"Semin. Cell Dev. Biol."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"162","DOI":"10.1016\/j.gde.2015.03.006","article-title":"Mechanisms of Myoblast Fusion during Muscle Development","volume":"32","author":"Kim","year":"2015","journal-title":"Curr. Opin. Genet. Dev."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1002\/wdev.182","article-title":"Specification of the Somatic Musculature in Drosophila","volume":"4","author":"Dobi","year":"2015","journal-title":"Wiley Interdiscip. Rev. Dev. Biol."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"705","DOI":"10.1016\/j.cub.2014.02.032","article-title":"Tension and Force-Resistant Attachment Are Essential for Myofibrillogenesis in Drosophila Flight Muscle","volume":"24","author":"Weitkunat","year":"2014","journal-title":"Curr. Biol."},{"key":"ref_56","doi-asserted-by":"crossref","unstructured":"Dasbiswas, K., Hu, S., Schnorrer, F., Safran, S.A., and Bershadsky, A.D. (2018). Ordering of Myosin II Filaments Driven by Mechanical Forces: Experiments and Theory. Philos. Trans. R. Soc. Lond. B Biol. Sci., 373.","DOI":"10.1098\/rstb.2017.0114"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"e34058","DOI":"10.7554\/eLife.34058","article-title":"A Transcriptomics Resource Reveals a Transcriptional Transition during Ordered Sarcomere Morphogenesis in Flight Muscle","volume":"7","author":"Spletter","year":"2018","journal-title":"Elife"},{"key":"ref_58","doi-asserted-by":"crossref","unstructured":"Loison, O., Weitkunat, M., Kaya-\u00c7opur, A., Nascimento Alves, C., Matzat, T., Spletter, M.L., Luschnig, S., Brasselet, S., Lenne, P.-F., and Schnorrer, F. (2018). Polarization-Resolved Microscopy Reveals a Muscle Myosin Motor-Independent Mechanism of Molecular Actin Ordering during Sarcomere Maturation. PLoS Biol., 16.","DOI":"10.1371\/journal.pbio.2004718"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"1261","DOI":"10.1242\/dev.140723","article-title":"Mechanical Tension and Spontaneous Muscle Twitching Precede the Formation of Cross-Striated Muscle in Vivo","volume":"144","author":"Weitkunat","year":"2017","journal-title":"Development"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"e63726","DOI":"10.7554\/eLife.63726","article-title":"The Hippo Pathway Controls Myofibril Assembly and Muscle Fiber Growth by Regulating Sarcomeric Gene Expression","volume":"10","author":"Marchiano","year":"2021","journal-title":"Elife"},{"key":"ref_61","first-page":"239","article-title":"Fiber Type Diversity in Skeletal Muscle Explored by Mass Spectrometry-Based Single Fiber Proteomics","volume":"35","author":"Schiaffino","year":"2020","journal-title":"Histol. Histopathol."},{"key":"ref_62","unstructured":"Vigoreaux, J.O. (2006). Functional and Ecological Effects of Isoform Variation in Insect Flight Muscle. Nature\u2019s Versatile Engine: Insect Flight Muscle Inside and Out, Molecular Biology Intelligence Unit; Springer."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"90","DOI":"10.1016\/j.yexcr.2013.10.007","article-title":"Transcriptional Regulation and Alternative Splicing Cooperate in Muscle Fiber-Type Specification in Flies and Mammals","volume":"321","author":"Spletter","year":"2014","journal-title":"Exp. Cell Res."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"1047","DOI":"10.1002\/bies.1150","article-title":"Genetics of the Drosophila Flight Muscle Myofibril: A Window into the Biology of Complex Systems","volume":"23","author":"Vigoreaux","year":"2001","journal-title":"Bioessays"},{"key":"ref_65","unstructured":"Vigoreaux, J.O. (2006). Comparative Physiology of Insect Flight Muscle. Nature\u2019s Versatile Engine: Insect Flight Muscle Inside and Out, Molecular Biology Intelligence Unit; Springer."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"2713","DOI":"10.1242\/jeb.203.18.2713","article-title":"Asynchronous Muscle: A Primer","volume":"203","author":"Josephson","year":"2000","journal-title":"J. Exp. Biol."},{"key":"ref_67","unstructured":"Vigoreaux, J.O. (2006). Insect Flight Muscle Chemomechanics. Nature\u2019s Versatile Engine: Insect Flight Muscle Inside and Out, Molecular Biology Intelligence Unit; Springer."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"e12068","DOI":"10.7554\/eLife.12068","article-title":"A Genome-Wide Resource for the Analysis of Protein Localisation in Drosophila","volume":"5","author":"Sarov","year":"2016","journal-title":"Elife"},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"760","DOI":"10.1091\/mbc.e16-07-0498","article-title":"Functional Redundancy and Nonredundancy between Two Troponin C Isoforms in Drosophila Adult Muscles","volume":"28","author":"Chechenova","year":"2017","journal-title":"MBoC"},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"1218","DOI":"10.1016\/j.bpj.2009.11.051","article-title":"The Mechanical Properties of Drosophila Jump Muscle Expressing Wild-Type and Embryonic Myosin Isoforms","volume":"98","author":"Eldred","year":"2010","journal-title":"Biophys. J."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/s41467-021-22058-7","article-title":"Myofibril and Mitochondria Morphogenesis Are Coordinated by a Mechanical Feedback Mechanism in Muscle","volume":"12","author":"Avellaneda","year":"2021","journal-title":"Nat. Commun."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"112486","DOI":"10.1016\/j.yexcr.2021.112486","article-title":"Marf-Mediated Mitochondrial Fusion Is Imperative for the Development and Functioning of Indirect Flight Muscles (IFMs) in Drosophila","volume":"399","author":"Katti","year":"2021","journal-title":"Exp. Cell Res."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"406","DOI":"10.1038\/nature10559","article-title":"Spalt Mediates an Evolutionarily Conserved Switch to Fibrillar Muscle Fate in Insects","volume":"479","author":"Distler","year":"2011","journal-title":"Nature"},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"443","DOI":"10.1006\/dbio.1993.1320","article-title":"Ultrastructure of Developing Flight Muscle in Drosophila. I. Assembly of Myofibrils","volume":"160","author":"Reedy","year":"1993","journal-title":"Dev. Biol."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"178","DOI":"10.15252\/embr.201439791","article-title":"The RNA-Binding Protein Arrest (Bruno) Regulates Alternative Splicing to Enable Myofibril Maturation in Drosophila Flight Muscle","volume":"16","author":"Spletter","year":"2015","journal-title":"EMBO Rep."},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1016\/B978-0-12-386499-4.00011-2","article-title":"Diversification of Muscle Types in Drosophila: Upstream and Downstream of Identity Genes","volume":"98","author":"Jagla","year":"2012","journal-title":"Curr. Top. Dev. Biol."},{"key":"ref_77","doi-asserted-by":"crossref","unstructured":"Maqbool, T., Soler, C., Jagla, T., Daczewska, M., Lodha, N., Palliyil, S., VijayRaghavan, K., and Jagla, K. (2006). Shaping Leg Muscles in Drosophila: Role of Ladybird, a Conserved Regulator of Appendicular Myogenesis. PLoS ONE, 1.","DOI":"10.1371\/journal.pone.0000122"},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"829","DOI":"10.1016\/S1534-5807(01)00089-2","article-title":"Myoblast Diversification and Ectodermal Signaling in Drosophila","volume":"1","author":"Sudarsan","year":"2001","journal-title":"Dev. Cell"},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"391","DOI":"10.1016\/S0012-1606(03)00255-0","article-title":"Control of Apterous by Vestigial Drives Indirect Flight Muscle Development in Drosophila","volume":"260","author":"Bernard","year":"2003","journal-title":"Dev. Biol."},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"664","DOI":"10.1016\/j.devcel.2012.08.004","article-title":"Extradenticle and Homothorax Control Adult Muscle Fiber Identity in Drosophila","volume":"23","author":"Bryantsev","year":"2012","journal-title":"Dev. Cell"},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"895","DOI":"10.1083\/jcb.201405058","article-title":"Arrest Is a Regulator of Fiber-Specific Alternative Splicing in the Indirect Flight Muscles of Drosophila","volume":"206","author":"Oas","year":"2014","journal-title":"J. Cell Biol."},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1093\/nar\/gkr648","article-title":"Regulated Functional Alternative Splicing in Drosophila","volume":"40","author":"Venables","year":"2012","journal-title":"Nucleic Acids Res."},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1006\/dbio.1996.9987","article-title":"Wingless Signaling Induces Nautilus Expression in the Ventral Mesoderm of the Drosophila Embryo","volume":"176","author":"Ranganayakulu","year":"1996","journal-title":"Dev. Biol."},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1016\/j.ydbio.2011.09.031","article-title":"Differential Requirements for Myocyte Enhancer Factor-2 during Adult Myogenesis in Drosophila","volume":"361","author":"Bryantsev","year":"2012","journal-title":"Dev. Biol."},{"key":"ref_85","doi-asserted-by":"crossref","first-page":"D132","DOI":"10.1093\/nar\/gks999","article-title":"Spliceosome Database: A Tool for Tracking Components of the Spliceosome","volume":"41","author":"Cvitkovic","year":"2013","journal-title":"Nucleic Acids Res."},{"key":"ref_86","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1038\/nature08799","article-title":"Systematic Genetic Analysis of Muscle Morphogenesis and Function in Drosophila","volume":"464","author":"Schnorrer","year":"2010","journal-title":"Nature"},{"key":"ref_87","doi-asserted-by":"crossref","unstructured":"Nikonova, E., Kamble, K., Mukherjee, A., Barz, C., Nongthomba, U., and Spletter, M.L. (2021). Rbfox1 Is Required for Myofibril Development and Maintaining Fiber-Type Specific Isoform Expression in Drosophila Muscles. bioRxiv.","DOI":"10.1101\/2021.05.09.443278"},{"key":"ref_88","doi-asserted-by":"crossref","first-page":"3442","DOI":"10.1093\/nar\/28.18.3442","article-title":"STRING: A Web-Server to Retrieve and Display the Repeatedly Occurring Neighbourhood of a Gene","volume":"28","author":"Snel","year":"2000","journal-title":"Nucleic Acids Res."},{"key":"ref_89","doi-asserted-by":"crossref","first-page":"D605","DOI":"10.1093\/nar\/gkaa1074","article-title":"The STRING Database in 2021: Customizable Protein-Protein Networks, and Functional Characterization of User-Uploaded Gene\/Measurement Sets","volume":"49","author":"Szklarczyk","year":"2021","journal-title":"Nucleic Acids Res."},{"key":"ref_90","doi-asserted-by":"crossref","first-page":"1232","DOI":"10.1080\/15476286.2017.1307484","article-title":"A Fly View on the Roles and Mechanisms of the M6A MRNA Modification and Its Players","volume":"14","author":"Lence","year":"2017","journal-title":"RNA Biol."},{"key":"ref_91","doi-asserted-by":"crossref","unstructured":"Lee, S., Wei, L., Zhang, B., Goering, R., Majumdar, S., Wen, J., Taliaferro, J.M., and Lai, E.C. (2021). ELAV\/Hu RNA Binding Proteins Determine Multiple Programs of Neural Alternative Splicing. PLoS Genet., 17.","DOI":"10.1371\/journal.pgen.1009439"},{"key":"ref_92","doi-asserted-by":"crossref","first-page":"676","DOI":"10.1038\/nmeth.2019","article-title":"Fiji: An Open-Source Platform for Biological-Image Analysis","volume":"9","author":"Schindelin","year":"2012","journal-title":"Nat. Methods"},{"key":"ref_93","doi-asserted-by":"crossref","unstructured":"Kao, S.-Y., Nikonova, E., Ravichandran, K., and Spletter, M.L. (2019). Dissection of Drosophila Melanogaster Flight Muscles for Omics Approaches. J. Vis. Exp.","DOI":"10.3791\/60309-v"},{"key":"ref_94","doi-asserted-by":"crossref","first-page":"5","DOI":"10.6026\/97320630002005","article-title":"Mfuzz: A Software Package for Soft Clustering of Microarray Data","volume":"2","author":"Kumar","year":"2007","journal-title":"Bioinformation"},{"key":"ref_95","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s13059-014-0550-8","article-title":"Moderated Estimation of Fold Change and Dispersion for RNA-Seq Data with DESeq2","volume":"15","author":"Love","year":"2014","journal-title":"Genome Biol."},{"key":"ref_96","doi-asserted-by":"crossref","first-page":"2008","DOI":"10.1101\/gr.133744.111","article-title":"Detecting Differential Usage of Exons from RNA-Seq Data","volume":"22","author":"Anders","year":"2012","journal-title":"Genome Res."},{"key":"ref_97","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/1471-2105-10-48","article-title":"GOrilla: A Tool for Discovery and Visualization of Enriched GO Terms in Ranked Gene Lists","volume":"10","author":"Eden","year":"2009","journal-title":"BMC Bioinform."},{"key":"ref_98","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1038\/75556","article-title":"Gene Ontology: Tool for the Unification of Biology","volume":"25","author":"Ashburner","year":"2000","journal-title":"Nat. Genet."},{"key":"ref_99","doi-asserted-by":"crossref","first-page":"8100","DOI":"10.1074\/jbc.M512468200","article-title":"Expression Profiling of a Hypercontraction-Induced Myopathy in Drosophila Suggests a Compensatory Cytoskeletal Remodeling Response","volume":"281","author":"Montana","year":"2006","journal-title":"J. Biol. Chem."},{"key":"ref_100","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1093\/genetics\/164.1.209","article-title":"Suppression of Muscle Hypercontraction by Mutations in the Myosin Heavy Chain Gene of Drosophila Melanogaster","volume":"164","author":"Nongthomba","year":"2003","journal-title":"Genetics"},{"key":"ref_101","doi-asserted-by":"crossref","first-page":"893","DOI":"10.3390\/biom5020893","article-title":"RNA-Binding Proteins: Splicing Factors and Disease","volume":"5","author":"Fredericks","year":"2015","journal-title":"Biomolecules"},{"key":"ref_102","doi-asserted-by":"crossref","first-page":"1615","DOI":"10.1017\/S1355838299991872","article-title":"Splicing Factor SF1 from Drosophila and Caenorhabditis: Presence of an N-Terminal RS Domain and Requirement for Viability","volume":"5","author":"Mazroui","year":"1999","journal-title":"RNA"},{"key":"ref_103","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1007\/978-1-4419-7005-3_9","article-title":"The Branchpoint Binding Protein: In and out of the Spliceosome Cycle","volume":"693","author":"Rymond","year":"2010","journal-title":"Adv. Exp. Med. Biol."},{"key":"ref_104","doi-asserted-by":"crossref","first-page":"689","DOI":"10.1101\/gr.229062.117","article-title":"HNRNPA1 Promotes Recognition of Splice Site Decoys by U2AF2 In Vivo","volume":"28","author":"Howard","year":"2018","journal-title":"Genome Res."},{"key":"ref_105","doi-asserted-by":"crossref","first-page":"314","DOI":"10.1016\/j.molcel.2011.11.033","article-title":"HnRNP A1 Proofreads 3\u2032 Splice Site Recognition by U2AF","volume":"45","author":"Tavanez","year":"2012","journal-title":"Mol. Cell"},{"key":"ref_106","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1007\/978-1-4939-6371-3_6","article-title":"A Guide to Genome-Wide In Vivo RNAi Applications in Drosophila","volume":"1478","author":"Schnorrer","year":"2016","journal-title":"Methods Mol. Biol."},{"key":"ref_107","doi-asserted-by":"crossref","unstructured":"Katzemich, A., Liao, K.A., Czerniecki, S., and Sch\u00f6ck, F. (2013). Alp\/Enigma Family Proteins Cooperate in Z-Disc Formation and Myofibril Assembly. PLoS Genet., 9.","DOI":"10.1371\/journal.pgen.1003342"},{"key":"ref_108","doi-asserted-by":"crossref","unstructured":"Liao, K.A., Gonz\u00e1lez-Morales, N., and Sch\u00f6ck, F. (2016). Zasp52, a Core Z-Disc Protein in Drosophila Indirect Flight Muscles, Interacts with \u03b1-Actinin via an Extended PDZ Domain. PLoS Genet., 12.","DOI":"10.1371\/journal.pgen.1006400"},{"key":"ref_109","doi-asserted-by":"crossref","first-page":"e201907026","DOI":"10.1083\/jcb.201907026","article-title":"Nanoscopy Reveals the Layered Organization of the Sarcomeric H-Zone and I-Band Complexes","volume":"219","author":"Szikora","year":"2020","journal-title":"J. Cell Biol."},{"key":"ref_110","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1080\/21541264.2018.1558677","article-title":"RNA Processing in Skeletal Muscle Biology and Disease","volume":"10","author":"Hinkle","year":"2019","journal-title":"Transcription"},{"key":"ref_111","doi-asserted-by":"crossref","first-page":"740","DOI":"10.1093\/hmg\/ddu492","article-title":"Nuclear Localization of MBNL1: Splicing-Mediated Autoregulation and Repression of Repeat-Derived Aberrant Proteins","volume":"24","author":"Kino","year":"2015","journal-title":"Hum. Mol. Genet."},{"key":"ref_112","doi-asserted-by":"crossref","first-page":"2766","DOI":"10.1093\/nar\/29.13.2766","article-title":"In Vivo Co-Localisation of MBNL Protein with DMPK Expanded-Repeat Transcripts","volume":"29","author":"Fardaei","year":"2001","journal-title":"Nucleic Acids Res."},{"key":"ref_113","doi-asserted-by":"crossref","first-page":"7820","DOI":"10.1074\/jbc.M005960200","article-title":"RNA CUG Repeats Sequester CUGBP1 and Alter Protein Levels and Activity of CUGBP1","volume":"276","author":"Timchenko","year":"2001","journal-title":"J. Biol. Chem."},{"key":"ref_114","doi-asserted-by":"crossref","first-page":"716","DOI":"10.1111\/brv.12674","article-title":"The Hallmarks of Myotonic Dystrophy Type 1 Muscle Dysfunction","volume":"96","author":"Ozimski","year":"2021","journal-title":"Biol. Rev."},{"key":"ref_115","doi-asserted-by":"crossref","unstructured":"Imbriano, C., and Molinari, S. (2018). Alternative Splicing of Transcription Factors Genes in Muscle Physiology and Pathology. Genes, 9.","DOI":"10.3390\/genes9020107"},{"key":"ref_116","doi-asserted-by":"crossref","first-page":"856","DOI":"10.1002\/mus.21789","article-title":"Altered MEF2 Isoforms in Myotonic Dystrophy and Other Neuromuscular Disorders","volume":"42","author":"Bachinski","year":"2010","journal-title":"Muscle Nerve"},{"key":"ref_117","doi-asserted-by":"crossref","first-page":"1150","DOI":"10.1038\/nsmb1316","article-title":"P38 MAPK Signaling Regulates Recruitment of Ash2L-Containing Methyltransferase Complexes to Specific Genes during Differentiation","volume":"14","author":"Rampalli","year":"2007","journal-title":"Nat. Struct. Mol. Biol."},{"key":"ref_118","doi-asserted-by":"crossref","first-page":"310","DOI":"10.1074\/jbc.M114.606277","article-title":"Alternative Splicing of MEF2C Pre-MRNA Controls Its Activity in Normal Myogenesis and Promotes Tumorigenicity in Rhabdomyosarcoma Cells","volume":"290","author":"Zhang","year":"2015","journal-title":"J. Biol. Chem."},{"key":"ref_119","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/ncomms10509","article-title":"E2F Function in Muscle Growth Is Necessary and Sufficient for Viability in Drosophila","volume":"7","author":"Zappia","year":"2016","journal-title":"Nat. Commun."},{"key":"ref_120","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1242\/jcs.110361","article-title":"Myosin Isoform Switching during Assembly of the Drosophila Flight Muscle Thick Filament Lattice","volume":"126","author":"Orfanos","year":"2013","journal-title":"J. Cell Sci."},{"key":"ref_121","doi-asserted-by":"crossref","first-page":"e50496","DOI":"10.7554\/eLife.50496","article-title":"Myofibril Diameter Is Set by a Finely Tuned Mechanism of Protein Oligomerization in Drosophila","volume":"8","author":"Xiao","year":"2019","journal-title":"Elife"},{"key":"ref_122","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1006\/dbio.1997.8833","article-title":"The Muscleblind Gene Participates in the Organization of Z-Bands and Epidermal Attachments of Drosophila Muscles and Is Regulated by Dmef2","volume":"195","author":"Artero","year":"1998","journal-title":"Dev. Biol."},{"key":"ref_123","doi-asserted-by":"crossref","unstructured":"Nir, R., Grossman, R., Paroush, Z., and Volk, T. (2012). Phosphorylation of the Drosophila Melanogaster RNA-Binding Protein HOW by MAPK\/ERK Enhances Its Dimerization and Activity. PLoS Genet., 8.","DOI":"10.1371\/journal.pgen.1002632"},{"key":"ref_124","doi-asserted-by":"crossref","first-page":"413","DOI":"10.1038\/nrc.2016.51","article-title":"RNA Splicing Factors as Oncoproteins and Tumour Suppressors","volume":"16","author":"Dvinge","year":"2016","journal-title":"Nat. Rev. Cancer"},{"key":"ref_125","doi-asserted-by":"crossref","first-page":"539","DOI":"10.1016\/j.molcel.2017.01.011","article-title":"Multilayered Control of Alternative Splicing Regulatory Networks by Transcription Factors","volume":"65","author":"Han","year":"2017","journal-title":"Mol. Cell"},{"key":"ref_126","doi-asserted-by":"crossref","unstructured":"Kitamura, K., and Nimura, K. (2021). Regulation of RNA Splicing: Aberrant Splicing Regulation and Therapeutic Targets in Cancer. Cells, 10.","DOI":"10.3390\/cells10040923"},{"key":"ref_127","doi-asserted-by":"crossref","first-page":"1591","DOI":"10.1101\/gr.246678.118","article-title":"RNA Components of the Spliceosome Regulate Tissue- and Cancer-Specific Alternative Splicing","volume":"29","author":"Dvinge","year":"2019","journal-title":"Genome Res."},{"key":"ref_128","doi-asserted-by":"crossref","first-page":"3718","DOI":"10.1074\/jbc.M112.401794","article-title":"The Drosophila Z-Disc Protein Z(210) Is an Adult Muscle Isoform of Zasp52, Which Is Required for Normal Myofibril Organization in Indirect Flight Muscles","volume":"288","author":"Chechenova","year":"2013","journal-title":"J. Biol. Chem."},{"key":"ref_129","doi-asserted-by":"crossref","unstructured":"Gonz\u00e1lez-Morales, N., Holenka, T.K., and Sch\u00f6ck, F. (2017). Filamin Actin-Binding and Titin-Binding Fulfill Distinct Functions in Z-Disc Cohesion. PLoS Genet., 13.","DOI":"10.1371\/journal.pgen.1006880"},{"key":"ref_130","unstructured":"Vigoreaux, J.O. (2006). Some Functions of Proteins from the Drosophila sallimus (sls) Gene. Nature\u2019s Versatile Engine: Insect Flight Muscle Inside and Out, Molecular Biology Intelligence Unit; Springer."},{"key":"ref_131","doi-asserted-by":"crossref","first-page":"160303","DOI":"10.1098\/rsob.160303","article-title":"Muscle Developmental Defects in Heterogeneous Nuclear Ribonucleoprotein A1 Knockout Mice","volume":"7","author":"Liu","year":"2017","journal-title":"Open Biol."},{"key":"ref_132","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1093\/jmcb\/mjz050","article-title":"Loss of HnRNP A1 in Murine Skeletal Muscle Exacerbates High-Fat Diet-Induced Onset of Insulin Resistance and Hepatic Steatosis","volume":"12","author":"Zhao","year":"2020","journal-title":"J. Mol. Cell Biol."},{"key":"ref_133","doi-asserted-by":"crossref","first-page":"5472","DOI":"10.1073\/pnas.1907297117","article-title":"HNRNPA1-Induced Spliceopathy in a Transgenic Mouse Model of Myotonic Dystrophy","volume":"117","author":"Li","year":"2020","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_134","doi-asserted-by":"crossref","first-page":"1868","DOI":"10.1093\/nar\/gkq1042","article-title":"Analysis of in Situ Pre-MRNA Targets of Human Splicing Factor SF1 Reveals a Function in Alternative Splicing","volume":"39","author":"Corioni","year":"2011","journal-title":"Nucleic Acids Res."},{"key":"ref_135","first-page":"10456","article-title":"Mammalian Splicing Factor SF1 Interacts with SURP Domains of U2 SnRNP-Associated Proteins","volume":"43","author":"Crisci","year":"2015","journal-title":"Nucleic Acids Res."}],"container-title":["Cells"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2073-4409\/10\/10\/2505\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T07:03:19Z","timestamp":1760166199000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2073-4409\/10\/10\/2505"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,9,22]]},"references-count":135,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2021,10]]}},"alternative-id":["cells10102505"],"URL":"https:\/\/doi.org\/10.3390\/cells10102505","relation":{"has-preprint":[{"id-type":"doi","id":"10.20944\/preprints202108.0137.v1","asserted-by":"object"}]},"ISSN":["2073-4409"],"issn-type":[{"value":"2073-4409","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,9,22]]}}}