{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T02:24:19Z","timestamp":1760235859024,"version":"build-2065373602"},"reference-count":82,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2021,9,28]],"date-time":"2021-09-28T00:00:00Z","timestamp":1632787200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Sixth Framework Programme","award":["HEALTH-2009_242038"],"award-info":[{"award-number":["HEALTH-2009_242038"]}]},{"DOI":"10.13039\/501100004587","name":"Instituto de Salud Carlos III","doi-asserted-by":"publisher","award":["RD16\/0011\/0037;  RD16\/0011\/0006","CB16\/11\/00403","RETICS-RTI2018-097604-B-I00;  PIC18\/00014 and PI20\/00247"],"award-info":[{"award-number":["RD16\/0011\/0037;  RD16\/0011\/0006","CB16\/11\/00403","RETICS-RTI2018-097604-B-I00;  PIC18\/00014 and PI20\/00247"]}],"id":[{"id":"10.13039\/501100004587","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004837","name":"Ministerio de Ciencia e Innovaci\u00f3n","doi-asserted-by":"publisher","award":["RTI2018-097604-B-I00  and  SAF2017-84324-C2-1-R"],"award-info":[{"award-number":["RTI2018-097604-B-I00  and  SAF2017-84324-C2-1-R"]}],"id":[{"id":"10.13039\/501100004837","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100012818","name":"Comunidad Aut\u00f3noma de Madrid","doi-asserted-by":"publisher","award":["S2017\/BMD-3692"],"award-info":[{"award-number":["S2017\/BMD-3692"]}],"id":[{"id":"10.13039\/100012818","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells"],"abstract":"<jats:p>Human cardiac progenitor cells (hCPC) are considered a good candidate in cell therapy for ischemic heart disease, demonstrating capacity to improve functional recovery after myocardial infarction (MI), both in small and large preclinical animal models. However, improvements are required in terms of cell engraftment and efficacy. Based on previously published reports, insulin-growth factor 1 (IGF-1) and hepatocyte growth factor (HGF) have demonstrated substantial cardioprotective, repair and regeneration activities, so they are good candidates to be evaluated in large animal model of MI. We have validated porcine cardiac progenitor cells (pCPC) and lentiviral vectors to overexpress IGF-1 (co-expressing eGFP) and HGF (co-expressing mCherry). pCPC were transduced and IGF1-eGFPpos and HGF-mCherrypos populations were purified by cell sorting and further expanded. Overexpression of IGF-1 has a limited impact on pCPC expression profile, whereas results indicated that pCPC-HGF-mCherry cultures could be counter selecting high expresser cells. In addition, pCPC-IGF1-eGFP showed a higher cardiogenic response, evaluated in co-cultures with decellularized extracellular matrix, compared with native pCPC or pCPC-HGF-mCherry. In vivo intracoronary co-administration of pCPC-IGF1-eGFP and pCPC-HFG-mCherry (1:1; 40 \u00d7 106\/animal), one week after the induction of an MI model in swine, revealed no significant improvement in cardiac function.<\/jats:p>","DOI":"10.3390\/cells10102571","type":"journal-article","created":{"date-parts":[[2021,9,28]],"date-time":"2021-09-28T12:29:14Z","timestamp":1632832154000},"page":"2571","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Intracoronary Delivery of Porcine Cardiac Progenitor Cells Overexpressing IGF-1 and HGF in a Pig Model of Sub-Acute Myocardial Infarction"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5621-1101","authenticated-orcid":false,"given":"Cristina","family":"Prat-Vidal","sequence":"first","affiliation":[{"name":"ICREC Research Program, Germans Trias i Pujol Health Science Research Institute, Can Ruti Campus, Heart Institute (iCor), Germans Trias i Pujol University Hospital, 08916 Badalona, Spain"},{"name":"CIBERCV, Instituto de Salud Carlos III, 28029 Madrid, Spain"},{"name":"Institut d\u2019Investigaci\u00f3 Biom\u00e8dica de Bellvitge-IDIBELL, 08908 L\u2019Hospitalet de Llobregat, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1740-7029","authenticated-orcid":false,"given":"Ver\u00f3nica","family":"Cris\u00f3stomo","sequence":"additional","affiliation":[{"name":"CIBERCV, Instituto de Salud Carlos III, 28029 Madrid, Spain"},{"name":"Jes\u00fas Us\u00f3n Minimally Invasive Surgery Center, 10071 C\u00e1ceres, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6078-4666","authenticated-orcid":false,"given":"Isabel","family":"Moscoso","sequence":"additional","affiliation":[{"name":"CIBERCV, Instituto de Salud Carlos III, 28029 Madrid, Spain"},{"name":"Cardiology Group, Center for Research in Molecular Medicine and Chronic Diseases (CIMUS), Universidade de Santiago de Compostela and Health Research Institute, University Clinical Hospital of Santiago de Compostela, 15782 Santiago de Compostela, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9031-2929","authenticated-orcid":false,"given":"Claudia","family":"B\u00e1ez-D\u00edaz","sequence":"additional","affiliation":[{"name":"CIBERCV, Instituto de Salud Carlos III, 28029 Madrid, Spain"},{"name":"Jes\u00fas Us\u00f3n Minimally Invasive Surgery Center, 10071 C\u00e1ceres, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1971-919X","authenticated-orcid":false,"given":"Virginia","family":"Blanco-Bl\u00e1zquez","sequence":"additional","affiliation":[{"name":"CIBERCV, Instituto de Salud Carlos III, 28029 Madrid, Spain"},{"name":"Jes\u00fas Us\u00f3n Minimally Invasive Surgery Center, 10071 C\u00e1ceres, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guadalupe","family":"G\u00f3mez-Mauricio","sequence":"additional","affiliation":[{"name":"Jes\u00fas Us\u00f3n Minimally Invasive Surgery Center, 10071 C\u00e1ceres, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guillermo","family":"Albericio","sequence":"additional","affiliation":[{"name":"Immunology and Oncology Department, National Center for Biotechnology, 28049 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Susana","family":"Aguilar","sequence":"additional","affiliation":[{"name":"Immunology and Oncology Department, National Center for Biotechnology, 28049 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9229-0022","authenticated-orcid":false,"given":"Mar\u00eda-Eugenia","family":"Fern\u00e1ndez-Santos","sequence":"additional","affiliation":[{"name":"CIBERCV, Instituto de Salud Carlos III, 28029 Madrid, Spain"},{"name":"Servicio de Cardiolog\u00eda, Hospital General Universitario Gregorio Mara\u00f1\u00f3n, Laboratorio Investigaci\u00f3n Traslacional en Cardiolog\u00eda (LITC), Unidad de Producci\u00f3n Celular-GMP (UPC-GMP), Instituto de Investigaci\u00f3n Sanitaria Gregorio Mara\u00f1\u00f3n (IiSGM), TERCEL, 28007 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5501-5275","authenticated-orcid":false,"given":"Francisco","family":"Fern\u00e1ndez-Avil\u00e9s","sequence":"additional","affiliation":[{"name":"CIBERCV, Instituto de Salud Carlos III, 28029 Madrid, Spain"},{"name":"Servicio de Cardiolog\u00eda, Hospital General Universitario Gregorio Mara\u00f1\u00f3n, Laboratorio Investigaci\u00f3n Traslacional en Cardiolog\u00eda (LITC), Unidad de Producci\u00f3n Celular-GMP (UPC-GMP), Instituto de Investigaci\u00f3n Sanitaria Gregorio Mara\u00f1\u00f3n (IiSGM), TERCEL, 28007 Madrid, Spain"},{"name":"Departamento de Medicina, Facultad de Medicina, Universidad Complutense de Madrid (UCM), 28040 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2138-988X","authenticated-orcid":false,"given":"Francisco M.","family":"S\u00e1nchez-Margallo","sequence":"additional","affiliation":[{"name":"CIBERCV, Instituto de Salud Carlos III, 28029 Madrid, Spain"},{"name":"Jes\u00fas Us\u00f3n Minimally Invasive Surgery Center, 10071 C\u00e1ceres, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Antoni","family":"Bayes-Genis","sequence":"additional","affiliation":[{"name":"ICREC Research Program, Germans Trias i Pujol Health Science Research Institute, Can Ruti Campus, Heart Institute (iCor), Germans Trias i Pujol University Hospital, 08916 Badalona, Spain"},{"name":"CIBERCV, Instituto de Salud Carlos III, 28029 Madrid, Spain"},{"name":"Cardiology Service, Germans Trias i Pujol University Hospital, 08916 Badalona, Spain"},{"name":"Department of Medicine, Universitat Aut\u00f2noma de Barcelona, 08193 Bellaterra, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Antonio","family":"Bernad","sequence":"additional","affiliation":[{"name":"Immunology and Oncology Department, National Center for Biotechnology, 28049 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2021,9,28]]},"reference":[{"key":"ref_1","first-page":"S52","article-title":"Myocyte death and renewal: Modern concepts of cardiac cellular homeostasis","volume":"4","author":"Ellison","year":"2007","journal-title":"Nat. Clin. Pract. Neurol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"2668","DOI":"10.1161\/CIRCULATIONAHA.118.035832","article-title":"Long Noncoding RNA CPR (Cardiomyocyte Proliferation Regulator) Regulates Cardiomyocyte Proliferation and Cardiac Repair","volume":"139","author":"Ponnusamy","year":"2019","journal-title":"Circulation"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1446","DOI":"10.1073\/pnas.1214608110","article-title":"Cardiomyocyte proliferation contributes to heart growth in young humans","volume":"110","author":"Mollova","year":"2013","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"418","DOI":"10.1038\/s41586-019-1191-6","article-title":"MicroRNA therapy stimulates uncontrolled cardiac repair after myocardial infarction in pigs","volume":"569","author":"Gabisonia","year":"2019","journal-title":"Nature"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1016\/j.stem.2019.11.010","article-title":"Arterial Sca1+ Vascular Stem Cells Generate De Novo Smooth Muscle for Artery Repair and Regeneration","volume":"26","author":"Tang","year":"2019","journal-title":"Cell Stem Cell"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"763","DOI":"10.1016\/S0092-8674(03)00687-1","article-title":"Adult Cardiac Stem Cells Are Multipotent and Support Myocardial Regeneration","volume":"114","author":"Beltrami","year":"2003","journal-title":"Cell"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"320","DOI":"10.1016\/j.stem.2008.03.010","article-title":"Lives of a Heart Cell: Tracing the Origins of Cardiac Progenitors","volume":"2","author":"Wang","year":"2008","journal-title":"Cell Stem Cell"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1016\/j.celrep.2014.06.004","article-title":"Endothelial Cells Contribute to Generation of Adult Ventricular Myocytes during Cardiac Homeostasis","volume":"8","author":"Fioret","year":"2014","journal-title":"Cell Rep."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"433","DOI":"10.1038\/nature11682","article-title":"Mammalian heart renewal by pre-existing cardiomyocytes","volume":"493","author":"Senyo","year":"2012","journal-title":"Nature"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"827","DOI":"10.1016\/j.cell.2013.07.039","article-title":"Adult c-kitpos Cardiac Stem Cells Are Necessary and Sufficient for Functional Cardiac Regeneration and Repair","volume":"154","author":"Ellison","year":"2013","journal-title":"Cell"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"2101","DOI":"10.1038\/cdd.2017.130","article-title":"Adult cardiac stem cells are multipotent and robustly myogenic: C-kit expression is necessary but not sufficient for their identification","volume":"24","author":"Vicinanza","year":"2017","journal-title":"Cell Death Differ."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"397","DOI":"10.1016\/j.stemcr.2013.09.004","article-title":"Sca1-Derived Cells Are a Source of Myocardial Renewal in the Murine Adult Heart","volume":"1","author":"Uchida","year":"2013","journal-title":"Stem Cell Rep."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"760","DOI":"10.1002\/emmm.201303626","article-title":"Stimulation of endogenous cardioblasts by exogenous cell therapy after myocardial infarction","volume":"6","author":"Malliaras","year":"2014","journal-title":"EMBO Mol. Med."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"765","DOI":"10.1016\/j.devcel.2019.01.017","article-title":"YAP Partially Reprograms Chromatin Accessibility to Directly Induce Adult Cardiogenesis In Vivo","volume":"48","author":"Monroe","year":"2019","journal-title":"Dev. Cell"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"680","DOI":"10.1161\/CIRCULATIONAHA.117.029343","article-title":"Cardiomyocyte Regeneration: A consensus statement","volume":"136","author":"Eschenhagen","year":"2017","journal-title":"Circulation"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1161\/CIRCULATIONAHA.111.042598","article-title":"Safety and Efficacy of Allogeneic Cell Therapy in Infarcted Rats Transplanted with Mismatched Cardiosphere-Derived Cells","volume":"125","author":"Malliaras","year":"2012","journal-title":"Circulation"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"2764","DOI":"10.1161\/CIRCULATIONAHA.113.002863","article-title":"Validation of contrast-enhanced magnetic resonance imaging to monitor regenerative efficacy after cell therapy in a porcine model of convalescent myocardial infarction","volume":"128","author":"Malliaras","year":"2013","journal-title":"Circulation"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1075","DOI":"10.1161\/CIRCULATIONAHA.108.816058","article-title":"Engraftment, Differentiation, and Functional Benefits of Autologous Cardiosphere-Derived Cells in Porcine Ischemic Cardiomyopathy","volume":"120","author":"Johnston","year":"2009","journal-title":"Circulation"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"2454","DOI":"10.1016\/j.jacc.2016.09.925","article-title":"Pim1 Kinase Overexpression Enhances ckit+ Cardiac Stem Cell Cardiac Repair Following Myocardial Infarction in Swine","volume":"68","author":"Kulandavelu","year":"2016","journal-title":"J. Am. Coll. Cardiol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"156","DOI":"10.1186\/s12967-015-0512-2","article-title":"Delayed administration of allogeneic cardiac stem cell therapy for acute myocardial infarction could ameliorate adverse remodeling: Experimental study in swine","volume":"13","author":"Crisostomo","year":"2015","journal-title":"J. Transl. Med."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"122","DOI":"10.1161\/CIRCULATIONAHA.112.001075","article-title":"Intracoronary Delivery of Autologous Cardiac Stem Cells Improves Cardiac Function in a Porcine Model of Chronic Ischemic Cardiomyopathy","volume":"128","author":"Bolli","year":"2013","journal-title":"Circulation"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"152","DOI":"10.1186\/s13287-019-1237-6","article-title":"Dose-dependent improvement of cardiac function in a swine model of acute myocardial infarction after intracoronary administration of allogeneic heart-derived cells","volume":"10","author":"Crisostomo","year":"2019","journal-title":"Stem Cell Res. Ther."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"895","DOI":"10.1016\/S0140-6736(12)60195-0","article-title":"Intracoronary cardiosphere-derived cells for heart regeneration after myocardial infarction (CADUCEUS): A prospective, randomised phase 1 trial","volume":"379","author":"Makkar","year":"2012","journal-title":"Lancet"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1016\/j.jacc.2013.08.724","article-title":"Intracoronary Cardiosphere-Derived Cells After Myocardial Infarction","volume":"63","author":"Malliaras","year":"2013","journal-title":"J. Am. Coll. Cardiol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"653","DOI":"10.1161\/CIRCRESAHA.116.304671","article-title":"Intracoronary Autologous Cardiac Progenitor Cell Transfer in Patients with Hypoplastic Left Heart Syndrome: The TICAP Pro-spective Phase 1 Controlled Trial","volume":"116","author":"Ishigami","year":"2015","journal-title":"Circ. Res."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"850","DOI":"10.1016\/j.mayocp.2014.02.014","article-title":"Breakthroughs in Cell Therapy for Heart Disease: Focus on Cardiosphere-Derived Cells","volume":"89","year":"2014","journal-title":"Mayo Clin. Proc."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"S54","DOI":"10.1161\/CIRCULATIONAHA.112.092627","article-title":"Administration of Cardiac Stem Cells in Patients with Ischemic Cardiomyopathy: The SCIPIO Trial","volume":"126","author":"Chugh","year":"2012","journal-title":"Circulation"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"835","DOI":"10.2217\/rme.09.64","article-title":"Assessing the value of autologous and allogeneic cells for regenerative medicine","volume":"4","author":"Mason","year":"2009","journal-title":"Regen. Med."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"3451","DOI":"10.1093\/eurheartj\/ehaa541","article-title":"Intracoronary ALLogeneic heart STem cells to Achieve myocardial Regeneration (ALLSTAR): A randomized, placebo-controlled, double-blinded trial","volume":"41","author":"Makkar","year":"2020","journal-title":"Eur. Heart J."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1161\/CIRCRESAHA.117.310651","article-title":"Rationale and Design of a Clinical Trial to Evaluate the Safety and Efficacy of Intracoronary Infusion of Allogeneic Human Cardiac Stem Cells in Patients with Acute Myocardial Infarction and Left Ventricular Dysfunction","volume":"121","author":"Plasencia","year":"2017","journal-title":"Circ. Res."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"579","DOI":"10.1161\/CIRCRESAHA.118.312823","article-title":"Safety and Efficacy of Intracoronary Infusion of Allogeneic Human Cardiac Stem Cells in Patients With ST-Segment Elevation Myocardial Infarction and Left Ventricular Dysfunction","volume":"123","author":"Bogaert","year":"2018","journal-title":"Circ. Res."},{"key":"ref_32","first-page":"2204","article-title":"Transplantation of cardiac progenitor cells ameliorates cardiac dysfunction after myocardial infarction in mice","volume":"119","author":"Matsuura","year":"2009","journal-title":"J. Clin. Investig."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1161\/CIRCULATIONAHA.109.871905","article-title":"Intracoronary Administration of Cardiac Progenitor Cells Alleviates Left Ventricular Dysfunction in Rats With a 30-Day-Old Infarction","volume":"121","author":"Tang","year":"2010","journal-title":"Circulation"},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Hong, K.U., Guo, Y., Li, Q.-H., Cao, P., Al-Maqtari, T., Vajravelu, B.N., Du, J., Book, M.J., Zhu, X., and Nong, Y. (2014). c-kit+ Cardiac Stem Cells Alleviate Post-Myocardial Infarction Left Ventricular Dysfunction Despite Poor Engraftment and Negligible Retention in the Recipient Heart. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0096725"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"730","DOI":"10.1093\/eurjhf\/hfq063","article-title":"New insights into paracrine mechanisms of human cardiac progenitor cells","volume":"12","author":"Maxeiner","year":"2010","journal-title":"Eur. J. Heart Fail."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"992","DOI":"10.1093\/cvr\/cvy055","article-title":"Cardioprotection by cardiac progenitor cell-secreted exosomes: Role of pregnancy-associated plasma protein-A","volume":"114","author":"Barile","year":"2018","journal-title":"Cardiovasc. Res."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"12490","DOI":"10.1038\/s41598-017-11976-6","article-title":"CXCL6 is an important paracrine factor in the pro-angiogenic human cardiac progenitor-like cell secretome","volume":"7","author":"Aguilar","year":"2017","journal-title":"Sci. Rep."},{"key":"ref_38","first-page":"1","article-title":"Exploring pericyte and cardiac stem cell secretome unveils new tactics for drug discovery","volume":"171","author":"Madeddu","year":"2016","journal-title":"Pharmacol. Ther."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"971","DOI":"10.1161\/CIRCRESAHA.109.210682","article-title":"Relative Roles of Direct Regeneration Versus Paracrine Effects of Human Cardiosphere-Derived Cells Transplanted Into Infarcted Mice","volume":"106","author":"Chimenti","year":"2010","journal-title":"Circ. Res."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"400","DOI":"10.1016\/j.acthis.2017.04.003","article-title":"ILK regulates MSCs survival and angiogenesis partially through AKT and mTOR signaling pathways","volume":"119","author":"Zeng","year":"2017","journal-title":"Acta Histochem."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"1487","DOI":"10.1161\/CIRCRESAHA.117.310738","article-title":"Engineering Cardiac Muscle Tissue","volume":"120","author":"Weinberger","year":"2017","journal-title":"Circ. Res."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"247","DOI":"10.3390\/biomedicines2040247","article-title":"HGF\/Met Axis in Heart Function and Cardioprotection","volume":"2","author":"Gallo","year":"2014","journal-title":"Biomedicines"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1016\/S0008-6363(01)00272-3","article-title":"A potential cardioprotective role of hepatocyte growth factor in myocardial infarction in rats","volume":"51","author":"Ueda","year":"2001","journal-title":"Cardiovasc. Res."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"902","DOI":"10.1016\/S0891-5849(01)00663-3","article-title":"Hepatocyte growth factor protects cardiac myocytes against oxidative stress-induced apoptosis","volume":"31","author":"Kitta","year":"2001","journal-title":"Free Radic. Biol. Med."},{"key":"ref_45","first-page":"4605","article-title":"Ad-HGF improves the cardiac remodeling of rat following myocardial infarction by upregulating autophagy and necroptosis and inhibiting apoptosis","volume":"8","author":"Liu","year":"2016","journal-title":"Am. J. Transl. Res."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"198","DOI":"10.1016\/S1674-8301(10)60029-2","article-title":"HGF percutaneous endocardial injection induces cardiomyocyte proliferation and rescues cardiac function in pigs","volume":"24","author":"Tao","year":"2010","journal-title":"J. Biomed. Res."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"674","DOI":"10.1016\/j.bbrc.2007.09.058","article-title":"The cardioprotective effect of mesenchymal stem cells is mediated by IGF-I and VEGF","volume":"363","author":"Sadat","year":"2007","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"1493","DOI":"10.1002\/stem.1660","article-title":"Insulin-Like Growth Factor Promotes Cardiac Lineage Induction In Vitro by Selective Expansion of Early Mesoderm","volume":"32","author":"Engels","year":"2014","journal-title":"Stem Cells"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"128","DOI":"10.1016\/j.tem.2013.12.002","article-title":"New insights into IGF-1 signaling in the heart","volume":"25","author":"Troncoso","year":"2014","journal-title":"Trends Endocrinol. Metab."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s13287-019-1544-y","article-title":"IGF-1 enhances BMSC viability, migration, and anti-apoptosis in myocardial infarction via secreted frizzled-related protein 2 pathway","volume":"11","author":"Lin","year":"2020","journal-title":"Stem Cell Res. Ther."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"1609","DOI":"10.3727\/096368915X690215","article-title":"Sustained IGF-1 Secretion by Adipose-Derived Stem Cells Improves Infarcted Heart Function","volume":"25","author":"Bagno","year":"2016","journal-title":"Cell Transplant."},{"key":"ref_52","first-page":"1","article-title":"Microencapsulated Insulin-Like Growth Factor-1 therapy improves cardiac function and reduces fibrosis in a porcine acute myocardial infarction model","volume":"10","author":"Abad","year":"2020","journal-title":"Sci. Rep."},{"key":"ref_53","doi-asserted-by":"crossref","unstructured":"Zhang, J., Wang, L.-L., Du, W., Yu, Y.-C., Ju, W.-Z., Man, Y.-L., Li, X.-R., Chen, Y., Wang, Z.-D., and Gu, W.-J. (2014). Hepatocyte Growth Factor Modification Enhances the Anti-Arrhythmic Properties of Human Bone Marrow-Derived Mesenchymal Stem Cells. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0111246"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1016\/j.biomaterials.2014.12.021","article-title":"Delivery of an engineered HGF fragment in an extracellular matrix-derived hydrogel prevents negative LV remodeling post-myocardial infarction","volume":"45","author":"Sonnenberg","year":"2015","journal-title":"Biomaterials"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"3012","DOI":"10.1002\/jbm.a.35442","article-title":"Enhanced engraftment and repairing ability of human adipose-derived stem cells, conveyed by pharmacologically active microcarriers continuously releasing HGF and IGF-1, in healing myocardial infarction in rats","volume":"103","author":"Savi","year":"2015","journal-title":"J. Biomed. Mater. Res. Part A"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1089\/hum.2011.094","article-title":"Improved Function of the Failing Rat Heart by Regulated Expression of Insulin-Like Growth Factor I via Intramuscular Gene Transfer","volume":"23","author":"Lai","year":"2012","journal-title":"Hum. Gene Ther."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"977","DOI":"10.1016\/j.jacc.2011.05.013","article-title":"Endogenous Cardiac Stem Cell Activation by Insulin-Like Growth Factor-1\/Hepatocyte Growth Factor Intracoronary Injection Fosters Survival and Regeneration of the Infarcted Pig Heart","volume":"58","author":"Ellison","year":"2011","journal-title":"J. Am. Coll. Cardiol."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"290","DOI":"10.1093\/cvr\/cvu208","article-title":"Natural killer cell crosstalk with allogeneic human cardiac-derived stem\/progenitor cells controls persistence","volume":"104","author":"Boukouaci","year":"2014","journal-title":"Cardiovasc. Res."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"451","DOI":"10.1161\/CIRCRESAHA.112.276501","article-title":"Allogenicity of Human Cardiac Stem\/Progenitor Cells Orchestrated by Programmed Death Ligand 1","volume":"112","author":"Lauden","year":"2013","journal-title":"Circ. Res."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"94","DOI":"10.1186\/s13287-016-0350-z","article-title":"Combined administration of mesenchymal stem cells overexpressing IGF-1 and HGF enhances neovascularization but moderately improves cardiac regeneration in a porcine model","volume":"7","author":"Moscoso","year":"2016","journal-title":"Stem Cell Res. Ther."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"800","DOI":"10.1093\/embo-reports\/kve174","article-title":"Restoration of telomerase activity rescues chromosomal instability and premature aging in Terc \u2212\/\u2212 mice with short telomeres","volume":"2","author":"Samper","year":"2001","journal-title":"EMBO Rep."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1038\/nm1684","article-title":"Perfusion-decellularized matrix: Using nature\u2019s platform to engineer a bioartificial heart","volume":"14","author":"Ott","year":"2008","journal-title":"Nat. Med."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1016\/j.biomaterials.2015.04.056","article-title":"Acellular human heart matrix: A critical step toward whole heart grafts","volume":"61","author":"Sanchez","year":"2015","journal-title":"Biomaterials"},{"key":"ref_64","first-page":"1","article-title":"Definition of a cell surface signature for human cardiac progenitor cells after comprehensive comparative transcriptomic and proteomic characterization","volume":"9","author":"Lopez","year":"2019","journal-title":"Sci. Rep."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"580","DOI":"10.1002\/term.1795","article-title":"Podocalyxin-like protein 1 is a relevant marker for human c-kitposcardiac stem cells","volume":"10","author":"Moscoso","year":"2013","journal-title":"J. Tissue Eng. Regen. Med."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"539","DOI":"10.1242\/jcs.114.3.539","article-title":"Characterization and chromosomal localization of JAM-1, a platelet receptor for a stimulatory monoclonal antibody","volume":"114","author":"Naik","year":"2001","journal-title":"J. Cell Sci."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1081\/RRS-120034252","article-title":"Signaling Pathways of the F11 Receptor (F11R; a.k.a. JAM-1, JAM-A) in Human Platelets: F11R Dimerization, Phosphorylation and Complex Formation with the Integrin GPIIIa","volume":"24","author":"Sobocka","year":"2004","journal-title":"J. Recept. Signal Transduct."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"575","DOI":"10.1042\/CS20140735","article-title":"JAM-A promotes wound healing by enhancing both homing and secretory activities of mesenchymal stem cells","volume":"129","author":"Wu","year":"2015","journal-title":"Clin. Sci."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1007\/s13577-013-0087-2","article-title":"RNA interference mediated JAM-A gene silencing promotes human epidermal stem cell proliferation","volume":"28","author":"Zhou","year":"2014","journal-title":"Hum. Cell"},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1038\/nature13623","article-title":"Jam1a\u2013Jam2a interactions regulate haematopoietic stem cell fate through Notch signalling","volume":"512","author":"Kobayashi","year":"2014","journal-title":"Nature"},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"724","DOI":"10.1186\/1471-2164-15-724","article-title":"Differences among brain tumor stem cell types and fetal neural stem cells in focal regions of histone modifications and DNA methylation, broad regions of modifications, and bivalent promoters","volume":"15","author":"Yoo","year":"2014","journal-title":"BMC Genom."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"1029","DOI":"10.1016\/j.stemcr.2014.10.010","article-title":"miR-133a Enhances the Protective Capacity of Cardiac Progenitors Cells after Myocardial Infarction","volume":"3","author":"Izarra","year":"2014","journal-title":"Stem Cell Rep."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"597","DOI":"10.21873\/anticanres.13153","article-title":"Basic Gene Expression Characteristics of Glioma Stem Cells and Human Glioblastoma","volume":"39","author":"Sakamoto","year":"2019","journal-title":"Anticancer Res."},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"6453","DOI":"10.2147\/OTT.S245336","article-title":"Overexpression of LRRC59 Is Associated with Poor Prognosis and Promotes Cell Proliferation and Invasion in Lung Adenocarcinoma","volume":"13","author":"Li","year":"2020","journal-title":"OncoTargets Ther."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1080\/14653240600855905","article-title":"Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement","volume":"8","author":"Dominici","year":"2006","journal-title":"Cytotherapy"},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"892","DOI":"10.1007\/s12015-019-09918-y","article-title":"The Inhibition of CD39 and CD73 Cell Surface Ectonucleotidases by Small Molecular Inhibitors Enhances the Mobilization of Bone Marrow Residing Stem Cells by Decreasing the Extracellular Level of Adenosine","volume":"15","author":"Adamiak","year":"2019","journal-title":"Stem Cell Rev. Rep."},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1080\/14728222.2019.1559829","article-title":"CD73 as a potential opportunity for cancer immunotherapy","volume":"23","author":"Ghalamfarsa","year":"2018","journal-title":"Expert Opin. Ther. Targets"},{"key":"ref_78","doi-asserted-by":"crossref","unstructured":"Piecewicz, S.M., Pandey, A., Roy, B., Xiang, S.H., Zetter, B.R., and Sengupta, S. (2012). Insulin-Like Growth Factors Promote Vasculogenesis in Embryonic Stem Cells. PLoS ONE, 7.","DOI":"10.1371\/journal.pone.0032191"},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"787","DOI":"10.3892\/mmr.2017.6639","article-title":"Inhibition of IGF-1 receptor kinase blocks the differentiation into cardiomyocyte-like cells of BMSCs induced by IGF-1","volume":"16","author":"Gong","year":"2017","journal-title":"Mol. Med. Rep."},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"218","DOI":"10.1016\/S0014-5793(00)01747-6","article-title":"Role of reactive oxygen species and phosphatidylinositol 3-kinase in cardiomyocyte differentiation of embryonic stem cells","volume":"476","author":"Sauer","year":"2000","journal-title":"FEBS Lett."},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"661","DOI":"10.1111\/cpr.12219","article-title":"HGF and IGF-1 promote protective effects of allogeneic BMSC transplantation in rabbit model of acute myocardial infarction","volume":"48","author":"Zhang","year":"2015","journal-title":"Cell Prolif."},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"1223","DOI":"10.1161\/CIRCRESAHA.115.307676","article-title":"Cardiac Stem Cell Treatment in Myocardial Infarction: A systematic review and me-ta-analysis of preclinical studies","volume":"118","author":"Zwetsloot","year":"2016","journal-title":"Circ. Res."}],"container-title":["Cells"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2073-4409\/10\/10\/2571\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T07:06:15Z","timestamp":1760166375000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2073-4409\/10\/10\/2571"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,9,28]]},"references-count":82,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2021,10]]}},"alternative-id":["cells10102571"],"URL":"https:\/\/doi.org\/10.3390\/cells10102571","relation":{},"ISSN":["2073-4409"],"issn-type":[{"type":"electronic","value":"2073-4409"}],"subject":[],"published":{"date-parts":[[2021,9,28]]}}}