{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T15:25:32Z","timestamp":1792596332509,"version":"4.5.0"},"reference-count":62,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2021,10,5]],"date-time":"2021-10-05T00:00:00Z","timestamp":1633392000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Key R&amp;D Program of China","award":["2018YFD0900200"],"award-info":[{"award-number":["2018YFD0900200"]}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31672649"],"award-info":[{"award-number":["31672649"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells"],"abstract":"<jats:p>Triploid oysters have poor gonadal development, which can not only bring higher economic benefits but also have a potential application in the genetic containment of aquaculture. However, the key factors that influence germ cell development in triploid oysters remain unclear. In this study, data-independent acquisition coupled to transcriptomics was applied to identify genes\/proteins related to sterility in triploid Crassostrea gigas. Eighty-four genes were differentially expressed at both the protein and mRNA levels between fertile and sterile females. For male oysters, 207 genes were differentially expressed in the transcriptomic and proteomic analysis. A large proportion of downregulated genes were related to cell division, which may hinder germ cell proliferation and cause apoptosis. In sterile triploid females, a primary cause of sterility may be downregulation in the expression levels of certain mitotic cell cycle-related genes. In sterile triploid males, downregulation of genes related to cell cycle and sperm motility indicated that the disruption of mitosis or meiosis and flagella defects may be linked with the blocking of spermatogenesis. Additionally, the genes upregulated in sterile oysters were mainly associated with the biosynthesis of glycogen and fat, suggesting that sterility in triploids stimulates the synthesis of glycogen and energy conservation in gonad tissue.<\/jats:p>","DOI":"10.3390\/cells10102668","type":"journal-article","created":{"date-parts":[[2021,10,7]],"date-time":"2021-10-07T09:24:04Z","timestamp":1633598644000},"page":"2668","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Integrated Proteomic and Transcriptomic Analysis of Gonads Reveal Disruption of Germ Cell Proliferation and Division, and Energy Storage in Glycogen in Sterile Triploid Pacific Oysters (Crassostrea gigas)"],"prefix":"10.3390","volume":"10","author":[{"given":"Chen","family":"Chen","sequence":"first","affiliation":[{"name":"Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao 266003, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong","family":"Yu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao 266003, China"},{"name":"Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266237, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qi","family":"Li","sequence":"additional","affiliation":[{"name":"Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao 266003, China"},{"name":"Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266237, China"},{"name":"Laboratory of Tropical Marine Germplasm Resources and Breeding Engineering, Sanya Oceanographic Institution, Ocean University of China, Sanya 572000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,10,5]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1038\/nrg.2017.26","article-title":"The evolutionary significance of polyploidy","volume":"18","author":"Mizrachi","year":"2017","journal-title":"Nat. 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