{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T13:57:47Z","timestamp":1790776667353,"version":"4.1.0"},"reference-count":46,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2021,11,18]],"date-time":"2021-11-18T00:00:00Z","timestamp":1637193600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"European Union's Horizon 2020 research and innovation programme","award":["Marie Sklodowska-Curie grant agreement No 665807"],"award-info":[{"award-number":["Marie Sklodowska-Curie grant agreement No 665807"]}]},{"DOI":"10.13039\/501100002915","name":"Fondation pour la Recherche M\u00e9dicale","doi-asserted-by":"publisher","award":["FRM Grant No. DBF20160635736"],"award-info":[{"award-number":["FRM Grant No. DBF20160635736"]}],"id":[{"id":"10.13039\/501100002915","id-type":"DOI","asserted-by":"publisher"}]},{"name":"ANR Unibac","award":["ANR-17-CE13-0010-01"],"award-info":[{"award-number":["ANR-17-CE13-0010-01"]}]},{"DOI":"10.13039\/501100003762","name":"Institut Pasteur","doi-asserted-by":"publisher","award":["PTR 245-19"],"award-info":[{"award-number":["PTR 245-19"]}],"id":[{"id":"10.13039\/501100003762","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells"],"abstract":"<jats:p>Antibiotics are well known drugs which, when present above certain concentrations, are able to inhibit the growth of certain bacteria. However, a growing body of evidence shows that even when present at lower doses (subMIC, for sub-minimal inhibitory concentration), unable to inhibit or affect microbial growth, antibiotics work as signaling molecules, affect gene expression and trigger important bacterial stress responses. However, how subMIC antibiotic signaling interplays with other well-known signaling networks in bacteria (and the consequences of such interplay) is not well understood. In this work, through transcriptomic and genetic approaches, we have explored how quorum-sensing (QS) proficiency of V. cholerae affects this pathogen\u2019s response to subMIC doses of the aminoglycoside tobramycin (TOB). We show that the transcriptomic signature of V. cholerae in response to subMIC TOB depends highly on the presence of QS master regulator HapR. In parallel, we show that subMIC doses of TOB are able to negatively interfere with the AI-2\/LuxS QS network of V. cholerae, which seems critical for survival to aminoglycoside treatment and TOB-mediated induction of SOS response in this species. This interplay between QS and aminoglycosides suggests that targeting QS signaling may be a strategy to enhance aminoglycoside efficacy in V. cholerae.<\/jats:p>","DOI":"10.3390\/cells10113227","type":"journal-article","created":{"date-parts":[[2021,11,19]],"date-time":"2021-11-19T02:43:09Z","timestamp":1637289789000},"page":"3227","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Interplay between Sublethal Aminoglycosides and Quorum Sensing: Consequences on Survival in V. cholerae"],"prefix":"10.3390","volume":"10","author":[{"given":"Andr\u00e9","family":"Carvalho","sequence":"first","affiliation":[{"name":"Unit\u00e9 Plasticit\u00e9 du G\u00e9nome Bact\u00e9rien, Institut Pasteur, UMR3525 CNRS, 75015 Paris, France"},{"name":"Coll\u00e8ge Doctoral, Sorbonne Universit\u00e9, 75005 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Evelyne","family":"Krin","sequence":"additional","affiliation":[{"name":"Unit\u00e9 Plasticit\u00e9 du G\u00e9nome Bact\u00e9rien, Institut Pasteur, UMR3525 CNRS, 75015 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chlo\u00e9","family":"Korlowski","sequence":"additional","affiliation":[{"name":"Unit\u00e9 Plasticit\u00e9 du G\u00e9nome Bact\u00e9rien, Institut Pasteur, UMR3525 CNRS, 75015 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6482-6002","authenticated-orcid":false,"given":"Didier","family":"Mazel","sequence":"additional","affiliation":[{"name":"Unit\u00e9 Plasticit\u00e9 du G\u00e9nome Bact\u00e9rien, Institut Pasteur, UMR3525 CNRS, 75015 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3477-2685","authenticated-orcid":false,"given":"Zeynep","family":"Baharoglu","sequence":"additional","affiliation":[{"name":"Unit\u00e9 Plasticit\u00e9 du G\u00e9nome Bact\u00e9rien, Institut Pasteur, UMR3525 CNRS, 75015 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,11,18]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"a012427b","DOI":"10.1101\/cshperspect.a012427","article-title":"Bacterial Quorum Sensing: Its Role in Virulence and Possibilities for Its Control","volume":"2","author":"Rutherford","year":"2012","journal-title":"Cold Spring Harb. 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