{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,16]],"date-time":"2026-03-16T21:04:08Z","timestamp":1773695048423,"version":"3.50.1"},"reference-count":28,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2021,11,26]],"date-time":"2021-11-26T00:00:00Z","timestamp":1637884800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Israel Ministry of Science and Technology","award":["MIA"],"award-info":[{"award-number":["MIA"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells"],"abstract":"<jats:p>We present a new method for the selection of individual sperm cells using a microfluidic device that automatically traps each cell in a separate microdroplet that then individually self-assembles with other microdroplets, permitting the controlled measurement of the cells using quantitative phase microscopy. Following cell trapping and droplet formation, we utilize quantitative phase microscopy integrated with bright-field imaging for individual sperm morphology and motility inspection. We then perform individual sperm selection using a single-cell micromanipulator, which is enhanced by the microdroplet-trapping procedure described above. This method can improve sperm selection for intracytoplasmic sperm injection, a common type of in vitro fertilization procedure.<\/jats:p>","DOI":"10.3390\/cells10123317","type":"journal-article","created":{"date-parts":[[2021,11,28]],"date-time":"2021-11-28T22:19:16Z","timestamp":1638137956000},"page":"3317","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Sperm Inspection for In Vitro Fertilization via Self-Assembled Microdroplet Formation and Quantitative Phase Microscopy"],"prefix":"10.3390","volume":"10","author":[{"given":"Yuval","family":"Atzitz","sequence":"first","affiliation":[{"name":"Department of Biomedical, Engineering Tel Aviv University, Tel Aviv 6997801, Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Matan","family":"Dudaie","sequence":"additional","affiliation":[{"name":"Department of Biomedical, Engineering Tel Aviv University, Tel Aviv 6997801, Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8590-8995","authenticated-orcid":false,"given":"Itay","family":"Barnea","sequence":"additional","affiliation":[{"name":"Department of Biomedical, Engineering Tel Aviv University, Tel Aviv 6997801, Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Natan","family":"Shaked","sequence":"additional","affiliation":[{"name":"Department of Biomedical, Engineering Tel Aviv University, Tel Aviv 6997801, Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2021,11,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Practice Committee of the American Society for Reproductive Medicine (2020). Definitions of infertility and recurrent pregnancy loss: A committee opinion. Fertil. Steril., 113, 533\u2013535.","DOI":"10.1016\/j.fertnstert.2019.11.025"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1967","DOI":"10.1038\/s41436-020-0916-0","article-title":"A genomics approach to male infertility","volume":"22","author":"Alhathal","year":"2020","journal-title":"Genet. Med."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1016\/S0015-0282(98)00146-0","article-title":"In vitro fertilization and pregnancy rates: The influence of sperm motility and morphology on IVF outcome","volume":"70","author":"Donnelly","year":"1998","journal-title":"Fertil. Steril."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Illiano, E., Trama, F., Zucchi, A., Iannitti, R.G., Fioretti, B., and Costantini, E. (2020). Resveratrol-Based Multivitamin Supplement Increases Sperm Concentration and Motility in Idiopathic Male Infertility: A Pilot Clinical Study. J. Clin. Med., 9.","DOI":"10.3390\/jcm9124017"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1172","DOI":"10.1016\/S0140-6736(97)10514-1","article-title":"Relation between semen quality and fertility: A population-based study of 430 first-pregnancy planners","volume":"352","author":"Bonde","year":"1998","journal-title":"Lancet"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1265","DOI":"10.1016\/S0140-6736(83)91148-0","article-title":"Current status of in-vitro fertilisation and implantation of human embryos","volume":"322","author":"Edwards","year":"1983","journal-title":"Lancet"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1016\/0140-6736(92)92425-F","article-title":"Pregnancies after intracytoplasmic injection of single spermatozoon into an oocyte","volume":"340","author":"Palermo","year":"1992","journal-title":"Lancet"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"152","DOI":"10.1016\/j.ymeth.2017.09.009","article-title":"Individual sperm selection by microfluidics integrated with interferometric phase microscopy","volume":"136","author":"Eravuchira","year":"2018","journal-title":"Methods"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"575","DOI":"10.1016\/S0277-9536(98)00386-4","article-title":"Psychological aspects of in vitro fertilization: A review","volume":"48","author":"Eugster","year":"1999","journal-title":"Soc. Sci. Med."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1093\/humupd\/dmh004","article-title":"A review of ten years experience of ICSI","volume":"10","author":"Devroey","year":"2004","journal-title":"Hum. Reprod. Update"},{"key":"ref_11","unstructured":"Gardner, D.K., Weissman, A., Howles, C.M., and Shoham, Z. (2009). Equipment and general technical aspects of micromanipulation of gametes and embryos. Textbook of Assisted Reproductive Technologies: Laboratory and Clinical Perspectives, Informa Healthcare. [3rd ed.]."},{"key":"ref_12","unstructured":"World Health Organization (2021). WHO Laboratory Manual for the Examination and Processing of Human Semen, World Health Organization. [6th ed.]."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1016\/j.fertnstert.2015.04.013","article-title":"Interferometric phase microscopy for label-free morphological evaluation of sperm cells","volume":"104","author":"Haifler","year":"2015","journal-title":"Fertil. Steril."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"893","DOI":"10.1002\/cyto.a.23189","article-title":"Automated analysis of individual sperm cells using stain-free interferometric phase microscopy and machine learning","volume":"91","author":"Mirsky","year":"2017","journal-title":"Cytom. Part A"},{"key":"ref_15","first-page":"1000190","article-title":"Label-Free quantitative imaging of sperm for in vitro fertilization using interferometric phase microscopy","volume":"4","author":"Mirsky","year":"2016","journal-title":"J. Fertil. Vitr.-IVF-Worldw. Reprod Med. Genet. Stem Cell Biol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1305","DOI":"10.1002\/jbio.201600186","article-title":"Localized measurements of physical parameters within human sperm cells obtained with wide-field interferometry","volume":"10","author":"Balberg","year":"2017","journal-title":"J. Biophotonics"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"18302","DOI":"10.1073\/pnas.2001754117","article-title":"Reproductive outcomes predicted by phase imaging with computational specificity of spermatozoon ultrastructure","volume":"117","author":"Kandel","year":"2020","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/s41598-020-69857-4","article-title":"High spatially sensitive quantitative phase imaging assisted with deep neural network for classification of human spermatozoa under stressed condition","volume":"10","author":"Butola","year":"2020","journal-title":"Sci. Rep."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1093\/jmicro\/dfaa053","article-title":"Transport of intensity equation method and its applications","volume":"70","author":"Mitome","year":"2021","journal-title":"Microscopy"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"e202000151","DOI":"10.1002\/jbio.202000151","article-title":"Label-free discrimination and selection of cancer cells from blood during flow using holography-induced dielectrophoresis","volume":"13","author":"Dudaie","year":"2020","journal-title":"J. Biophotonics"},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Hughes, M.P., and Hoettges, K.F. (2010). The application of microfluidics in biology. Microengineering in Biotechnology, Humana Press.","DOI":"10.1007\/978-1-60327-106-6"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"3374","DOI":"10.1002\/smll.201300020","article-title":"Exhaustion of racing sperm in nature-mimicking microfluidic channels during sorting","volume":"9","author":"Tasoglu","year":"2013","journal-title":"Small"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1671","DOI":"10.1021\/ac020579e","article-title":"Passively driven integrated microfluidic system for separation of motile sperm","volume":"75","author":"Cho","year":"2003","journal-title":"Anal. Chem."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1900188","DOI":"10.1002\/adbi.201900188","article-title":"Single-cell analysis using droplet microfluidics","volume":"4","author":"Rivello","year":"2020","journal-title":"Adv. Biosyst."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1202","DOI":"10.1016\/j.cell.2015.05.002","article-title":"Highly parallel genome-wide expression profiling of individual cells using nanoliter droplets","volume":"161","author":"Macosko","year":"2015","journal-title":"Cell"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1016\/j.trac.2014.03.006","article-title":"Droplet microfluidics for single-molecule and single-cell analysis in cancer research, diagnosis and therapy","volume":"58","author":"Kang","year":"2014","journal-title":"TrAC Trends Anal. Chem."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"5701","DOI":"10.1364\/OE.21.005701","article-title":"Compact and portable low-coherence interferometer with off-axis geometry for quantitative phase microscopy and nanoscopy","volume":"21","author":"Girshovitz","year":"2013","journal-title":"Opt. Express"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/s41467-021-23773-x","article-title":"Curvature in the reproductive tract alters sperm\u2013surface interactions","volume":"12","author":"Raveshi","year":"2021","journal-title":"Nat. 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