{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T03:10:44Z","timestamp":1790997044189,"version":"4.1.0"},"reference-count":54,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2022,1,6]],"date-time":"2022-01-06T00:00:00Z","timestamp":1641427200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001407","name":"Department of Biotechnology","doi-asserted-by":"publisher","award":["BT\/RLF\/Re-entry\/28\/2014"],"award-info":[{"award-number":["BT\/RLF\/Re-entry\/28\/2014"]}],"id":[{"id":"10.13039\/501100001407","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006143","name":"Department of Science and Technology","doi-asserted-by":"publisher","award":["ECR\/2017\/001274"],"award-info":[{"award-number":["ECR\/2017\/001274"]}],"id":[{"id":"10.13039\/501100006143","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells"],"abstract":"<jats:p>Neutrophil extracellular traps (NETs) are associated with multiple disease pathologies including sepsis, asthma, rheumatoid arthritis, cancer, systemic lupus erythematosus, acute respiratory distress syndrome, and COVID-19. NETs, being a disintegrated death form, suffered inconsistency in their identification, nomenclature, and quantifications that hindered therapeutic approaches using NETs as a target. Multiple strategies including microscopy, ELISA, immunoblotting, flow cytometry, and image-stream-based methods have exhibited drawbacks such as being subjective, non-specific, error-prone, and not being high throughput, and thus demand the development of innovative and efficient approaches for their analyses. Here, we established an imaging and computational algorithm using high content screening (HCS)\u2014cellomics platform that aid in easy, rapid, and specific detection as well as analyses of NETs. This method employed membrane-permeable and impermeable DNA dyes in situ to identify NET-forming cells. Automated algorithm-driven single-cell analysis of change in nuclear morphology, increase in nuclear area, and change in intensities provided precise detection of NET-forming cells and eliminated user bias with other cell death modalities. Further combination with Annexin V staining in situ detected specific death pathway, e.g., apoptosis, and thus, discriminated between NETs, apoptosis, and necrosis. Our approach does not utilize fixation and permeabilization steps that disturb NETs, and thus, allows the time-dependent monitoring of NETs. Together, this specific imaging-based high throughput method for NETs analyses may provide a good platform for the discovery of potential inhibitors of NET formation and\/or agents to modulate neutrophil death, e.g., NETosis-apoptosis switch, as an alternative strategy to enhance the resolution of inflammation.<\/jats:p>","DOI":"10.3390\/cells11020191","type":"journal-article","created":{"date-parts":[[2022,1,7]],"date-time":"2022-01-07T01:15:26Z","timestamp":1641518126000},"page":"191","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["An Imaging and Computational Algorithm for Efficient Identification and Quantification of Neutrophil Extracellular Traps"],"prefix":"10.3390","volume":"11","author":[{"given":"Apurwa","family":"Singhal","sequence":"first","affiliation":[{"name":"Pharmacology Division, CSIR\u2014Central Drug Research Institute, Lucknow 226021, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shubhi","family":"Yadav","sequence":"additional","affiliation":[{"name":"Pharmacology Division, CSIR\u2014Central Drug Research Institute, Lucknow 226021, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tulika","family":"Chandra","sequence":"additional","affiliation":[{"name":"Transfusion Medicine Department, King George\u2019s Medical University, Lucknow 226021, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4261-445X","authenticated-orcid":false,"given":"Shrikant R.","family":"Mulay","sequence":"additional","affiliation":[{"name":"Pharmacology Division, CSIR\u2014Central Drug Research Institute, Lucknow 226021, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anil Nilkanth","family":"Gaikwad","sequence":"additional","affiliation":[{"name":"Pharmacology Division, CSIR\u2014Central Drug Research Institute, Lucknow 226021, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3835-8935","authenticated-orcid":false,"given":"Sachin","family":"Kumar","sequence":"additional","affiliation":[{"name":"Pharmacology Division, CSIR\u2014Central Drug Research Institute, Lucknow 226021, India"},{"name":"Academy of Scientific and Innovative Research (AcSIR), Postal Staff College Area, Sector 19, Kamla Nehru Nagar, Ghaziabad 201002, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,1,6]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1038\/nri1785","article-title":"Neutrophils and immunity: Challenges and opportunities","volume":"6","author":"Nathan","year":"2006","journal-title":"Nat. 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