{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,11]],"date-time":"2026-06-11T15:16:15Z","timestamp":1781190975350,"version":"3.54.1"},"reference-count":54,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2022,2,1]],"date-time":"2022-02-01T00:00:00Z","timestamp":1643673600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["SFB 974 A03"],"award-info":[{"award-number":["SFB 974 A03"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["AH 92\/8-1"],"award-info":[{"award-number":["AH 92\/8-1"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002347","name":"Federal Ministry of Education and Research","doi-asserted-by":"publisher","award":["GeNeRARe; grant number:, 01GM1902C"],"award-info":[{"award-number":["GeNeRARe; grant number:, 01GM1902C"]}],"id":[{"id":"10.13039\/501100002347","id-type":"DOI","asserted-by":"publisher"}]},{"name":"the European Network on Noonan Syndrome and Related Disorders","award":["NSEuroNet; grant number: 01GM1621B"],"award-info":[{"award-number":["NSEuroNet; grant number: 01GM1621B"]}]},{"name":"the Research Committee of the Medical Faculty of the Heinrich Heine University","award":["9772764 to MRA and MCK"],"award-info":[{"award-number":["9772764 to MRA and MCK"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells"],"abstract":"<jats:p>Embryonic stem cell-expressed Ras (ERas) is an atypical constitutively active member of the Ras family and controls distinct signaling pathways, which are critical, for instance, for the maintenance of quiescent hepatic stellate cells (HSCs). Unlike classical Ras paralogs, ERas has a unique N-terminal extension (Nex) with as yet unknown function. In this study, we employed affinity pull-down and quantitative liquid chromatography-tandem mass spectrometry (LC\u2013MS\/MS) analyses and identified 76 novel binding proteins for human and rat ERas Nex peptides, localized in different subcellular compartments and involved in various cellular processes. One of the identified Nex-binding proteins is the nonmitochondrial, cytosolic arginase 1 (ARG1), a key enzyme of the urea cycle and involved in the de novo synthesis of polyamines, such as spermidine and spermine. Here, we show, for the first time, a high-affinity interaction between ERas Nex and purified ARG1 as well as their subcellular colocalization. The inhibition of ARG1 activity strikingly accelerates the activation of HSCs ex vivo, suggesting a central role of ARG1 activity in the maintenance of HSC quiescence.<\/jats:p>","DOI":"10.3390\/cells11030508","type":"journal-article","created":{"date-parts":[[2022,2,1]],"date-time":"2022-02-01T22:16:18Z","timestamp":1643753778000},"page":"508","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Physical Interaction between Embryonic Stem Cell-Expressed Ras (ERas) and Arginase-1 in Quiescent Hepatic Stellate Cells"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4653-7263","authenticated-orcid":false,"given":"Silke","family":"Pudewell","sequence":"first","affiliation":[{"name":"Institute of Biochemistry and Molecular Biology II, Medical Faculty and University Hospital D\u00fcsseldorf, Heinrich Heine University D\u00fcsseldorf, 40225 D\u00fcsseldorf, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jana","family":"Lissy","sequence":"additional","affiliation":[{"name":"Institute of Biochemistry and Molecular Biology II, Medical Faculty and University Hospital D\u00fcsseldorf, Heinrich Heine University D\u00fcsseldorf, 40225 D\u00fcsseldorf, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hossein","family":"Nakhaeizadeh","sequence":"additional","affiliation":[{"name":"Institute of Biochemistry and Molecular Biology II, Medical Faculty and University Hospital D\u00fcsseldorf, Heinrich Heine University D\u00fcsseldorf, 40225 D\u00fcsseldorf, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohamed S.","family":"Taha","sequence":"additional","affiliation":[{"name":"Institute of Biochemistry and Molecular Biology II, Medical Faculty and University Hospital D\u00fcsseldorf, Heinrich Heine University D\u00fcsseldorf, 40225 D\u00fcsseldorf, Germany"},{"name":"Research on Children with Special Needs Department, Medical Research and Clinical Studies Institute, National Research Centre, 12622 Dokki, Cairo, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammad","family":"Akbarzadeh","sequence":"additional","affiliation":[{"name":"Institute of Biochemistry and Molecular Biology II, Medical Faculty and University Hospital D\u00fcsseldorf, Heinrich Heine University D\u00fcsseldorf, 40225 D\u00fcsseldorf, Germany"},{"name":"Department of Chemical Biology, Max-Planck Institute of Molecular Physiology, Otto-Hahn-Strasse 11, 44227 Dortmund, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Soheila","family":"Rezaei Adariani","sequence":"additional","affiliation":[{"name":"Institute of Biochemistry and Molecular Biology II, Medical Faculty and University Hospital D\u00fcsseldorf, Heinrich Heine University D\u00fcsseldorf, 40225 D\u00fcsseldorf, Germany"},{"name":"Department of Chemical Biology, Max-Planck Institute of Molecular Physiology, Otto-Hahn-Strasse 11, 44227 Dortmund, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saeideh","family":"Nakhaei-Rad","sequence":"additional","affiliation":[{"name":"Institute of Biochemistry and Molecular Biology II, Medical Faculty and University Hospital D\u00fcsseldorf, Heinrich Heine University D\u00fcsseldorf, 40225 D\u00fcsseldorf, Germany"},{"name":"Stem Cell Biology and Regenerative Medicine Research Group, Institute of Biotechnology, Ferdowsi University of Mashhad, Mashhad 9177948974, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junjie","family":"Li","sequence":"additional","affiliation":[{"name":"Myocardial Infarction Research Laboratory, Department of Cardiology, Pulmonology, and Vascular Medicine, Medical Faculty and University Hospital D\u00fcsseldorf, Heinrich Heine University D\u00fcsseldorf, 40225 D\u00fcsseldorf, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0782-1998","authenticated-orcid":false,"given":"Claus","family":"Kordes","sequence":"additional","affiliation":[{"name":"Clinic of Gastroenterology, Hepatology and Infectious Diseases, Medical Faculty of the Heinrich-Heine University, 40225 D\u00fcsseldorf, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dieter","family":"H\u00e4ussinger","sequence":"additional","affiliation":[{"name":"Clinic of Gastroenterology, Hepatology and Infectious Diseases, Medical Faculty of the Heinrich-Heine University, 40225 D\u00fcsseldorf, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roland P.","family":"Piekorz","sequence":"additional","affiliation":[{"name":"Institute of Biochemistry and Molecular Biology II, Medical Faculty and University Hospital D\u00fcsseldorf, Heinrich Heine University D\u00fcsseldorf, 40225 D\u00fcsseldorf, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miriam M.","family":"Cortese-Krott","sequence":"additional","affiliation":[{"name":"Myocardial Infarction Research Laboratory, Department of Cardiology, Pulmonology, and Vascular Medicine, Medical Faculty and University Hospital D\u00fcsseldorf, Heinrich Heine University D\u00fcsseldorf, 40225 D\u00fcsseldorf, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2034-8894","authenticated-orcid":false,"given":"Mohammad Reza","family":"Ahmadian","sequence":"additional","affiliation":[{"name":"Institute of Biochemistry and Molecular Biology II, Medical Faculty and University Hospital D\u00fcsseldorf, Heinrich Heine University D\u00fcsseldorf, 40225 D\u00fcsseldorf, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,2,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"541","DOI":"10.1038\/nature01646","article-title":"Role of ERas in promoting tumour-like properties in mouse embryonic stem cells","volume":"423","author":"Takahashi","year":"2003","journal-title":"Nature"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1011","DOI":"10.3892\/ijo_00000752","article-title":"Resistance to chemotherapeutic agents and promotion of transforming activity mediated by embryonic stem cell-expressed Ras (ERas) signal in neuroblastoma cells","volume":"37","author":"Aoyama","year":"2010","journal-title":"Int. J. Oncol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1594","DOI":"10.1038\/s41388-017-0031-0","article-title":"Insertional mutagenesis in a HER2-positive breast cancer model reveals ERAS as a driver of cancer and therapy resistance","volume":"37","author":"Ikink","year":"2018","journal-title":"Oncogene"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"955","DOI":"10.2353\/ajpath.2010.091056","article-title":"Role of ES cell-expressed Ras (ERas) in tumorigenicity of gastric cancer","volume":"177","author":"Kubota","year":"2010","journal-title":"Am. J. Pathol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"E536","DOI":"10.1073\/pnas.1418163112","article-title":"BRAF inhibitor resistance mediated by the AKT pathway in an oncogenic BRAF mouse melanoma model","volume":"112","author":"Perna","year":"2015","journal-title":"Proc. Natl. Acad. Sci. 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Chem."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1152\/physrev.00013.2007","article-title":"Hepatic Stellate Cells: Protean, Multifunctional, and Enigmatic Cells of the Liver","volume":"88","author":"Friedman","year":"2008","journal-title":"Physiol. Rev."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1874","DOI":"10.1172\/JCI66027","article-title":"Hepatic stem cell niches","volume":"123","author":"Kordes","year":"2013","journal-title":"J. Clin. Investig."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"290","DOI":"10.1159\/000343368","article-title":"Hepatic Stellate Cells Support Hematopoiesis and are Liver-Resident Mesenchymal Stem Cells","volume":"31","author":"Kordes","year":"2013","journal-title":"Cell. Physiol. Biochem."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"521","DOI":"10.1038\/s41581-018-0032-4","article-title":"Pericytes in the renal vasculature: Roles in health and disease","volume":"14","author":"Shaw","year":"2018","journal-title":"Nat. Rev. Nephrol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1021","DOI":"10.1515\/hsz-2021-0180","article-title":"Hepatic stellate cells: Current state and open questions","volume":"402","author":"Kordes","year":"2021","journal-title":"Biol. Chem."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"16549","DOI":"10.1073\/pnas.0404678101","article-title":"Lhx2-\/- mice develop liver fibrosis","volume":"101","author":"Wandzioch","year":"2004","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"655","DOI":"10.1097\/01.LAB.0000069036.63405.5C","article-title":"Apoptotic body engulfment by a human stellate cell line is profibrogenic","volume":"83","author":"Canbay","year":"2003","journal-title":"Lab. Investig."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1016\/j.ymgme.2003.10.012","article-title":"Arginases I and II: Do their functions overlap?","volume":"81","author":"Cederbaum","year":"2004","journal-title":"Mol. Genet. Metab."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"15049","DOI":"10.1073\/pnas.1307058110","article-title":"Arginase regulates red blood cell nitric oxide synthase and export of cardioprotective nitric oxide bioactivity","volume":"110","author":"Yang","year":"2013","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"2812","DOI":"10.1093\/jn\/134.10.2812S","article-title":"Novel roles for arginase in cell survival, regeneration, and translation in the central nervous system","volume":"134","author":"Lange","year":"2004","journal-title":"J. Nutr."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"241","DOI":"10.3109\/07853899109148056","article-title":"Polyamines: From molecular biology to clinical applications","volume":"23","author":"Alhonen","year":"1991","journal-title":"Ann. Med."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1042\/bj1660105","article-title":"Metabolism of arginine in lactating rat mammary gland","volume":"166","author":"Mezl","year":"1977","journal-title":"Biochem. J."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"906","DOI":"10.1111\/j.1440-1681.2007.04638.x","article-title":"Arginase: A critical regulator of nitric oxide synthesis and vascular function","volume":"34","author":"Durante","year":"2007","journal-title":"Clin. Exp. Pharmacol. Physiol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"638","DOI":"10.1111\/j.1476-5381.2009.00291.x","article-title":"Arginase: An emerging key player in the mammalian immune system: Review","volume":"158","author":"Munder","year":"2009","journal-title":"Br. J. Pharmacol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"707","DOI":"10.1146\/annurev.ph.57.030195.003423","article-title":"Nitric Oxide Synthases: Properties and Catalytic Mechanism","volume":"57","author":"Griffi","year":"1995","journal-title":"Ann. Rev. Physiol."},{"key":"ref_24","first-page":"181","article-title":"Human arginase isozymes","volume":"13","author":"Grody","year":"1987","journal-title":"Isozymes"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1006\/abbi.1994.1276","article-title":"Rat liver arginase: Kinetic mechanism, alternate substrates, and inhibitors","volume":"312","author":"Reczkowski","year":"1994","journal-title":"Arch. Biochem. Biophys."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1002\/humu.21377","article-title":"Germline KRAS mutations cause aberrant biochemical and physical properties leading to developmental disorders","volume":"32","author":"Gremer","year":"2011","journal-title":"Hum. Mutat."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1016\/0022-1759(94)90027-2","article-title":"Determination of arginase activity in macrophages: A micromethod","volume":"174","author":"Corraliza","year":"1994","journal-title":"J. Immunol. Methods"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"2339","DOI":"10.1021\/pr401157k","article-title":"High-fat diet induced isoform changes of the Parkinson\u2019s disease protein DJ-1","volume":"13","author":"Poschmann","year":"2014","journal-title":"J. Proteome Res."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"5116","DOI":"10.1073\/pnas.091062498","article-title":"Significance analysis of microarrays applied to the ionizing radiation response","volume":"98","author":"Tusher","year":"2001","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"D183","DOI":"10.1093\/nar\/gkw1138","article-title":"PANTHER version 11: Expanded annotation data from Gene Ontology and Reactome pathways, and data analysis tool enhancements","volume":"45","author":"Mi","year":"2017","journal-title":"Nucleic Acids Res."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Taha, M.S., Nouri, K., Milroy, L.G., Moll, J.M., Herrmann, C., Brunsveld, L., Piekorz, R.P., and Ahmadian, M.R. (2014). Subcellular Fractionation and Localization Studies Reveal a Direct Interaction of the Fragile X Mental Retardation Protein (FMRP) with Nucleolin. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0091465"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"15892","DOI":"10.1074\/jbc.M115.640607","article-title":"The function of embryonic stem cell-expressed RAS (E-RAS), a unique RAS family member, correlates with its additional motifs and its structural properties","volume":"290","author":"Nakhaeizadeh","year":"2015","journal-title":"J. Biol. Chem."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1149","DOI":"10.1038\/nprot.2013.055","article-title":"Imaging of neutral lipids by oil red O for analyzing the metabolic status in health and disease","volume":"8","author":"Mehlem","year":"2013","journal-title":"Nat. Protoc."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"G713","DOI":"10.1152\/ajpgi.00251.2017","article-title":"Hepatic stellate cells retain retinoid-laden lipid droplets after cellular transdifferentiation into activated myofibroblasts","volume":"315","author":"Jophlin","year":"2018","journal-title":"Am. J. Physiol. Gastrointest. Liver Physiol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"130","DOI":"10.1080\/10409238.2018.1431605","article-title":"Structural fingerprints, interactions, and signaling networks of RAS family proteins beyond RAS isoforms","volume":"53","author":"Haghighi","year":"2018","journal-title":"Crit. Rev. Biochem. Mol. Biol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1002\/iub.1237","article-title":"The function of spermine","volume":"66","author":"Pegg","year":"2014","journal-title":"IUBMB Life"},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Hesterberg, R., Cleveland, J., and Epling-Burnette, P. (2018). Role of Polyamines in Immune Cell Functions. Med. Sci., 6.","DOI":"10.3390\/medsci6010022"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"160","DOI":"10.4161\/auto.6.1.10600","article-title":"Spermidine: A novel autophagy inducer and longevity elixir","volume":"6","author":"Madeo","year":"2010","journal-title":"Autophagy"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"102083","DOI":"10.1016\/j.npep.2020.102083","article-title":"Spermidine, an autophagy inducer, as a therapeutic strategy in neurological disorders","volume":"83","author":"Ghosh","year":"2020","journal-title":"Neuropeptides"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"575","DOI":"10.1007\/s00726-009-0418-5","article-title":"\u03b1-Methylspermidine protects against carbon tetrachloride-induced hepatic and pancreatic damage","volume":"38","author":"Sinervirta","year":"2010","journal-title":"Amino Acids"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"869","DOI":"10.1007\/s00726-012-1413-9","article-title":"Modulation of ethanol effect on hepatocyte proliferation by polyamines","volume":"44","author":"Do","year":"2013","journal-title":"Amino Acids"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"4517","DOI":"10.4161\/cc.22772","article-title":"A role for polyamine regulators in ESC self-renewal","volume":"11","author":"Zhao","year":"2012","journal-title":"Cell Cycle"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"1170","DOI":"10.1002\/stem.2830","article-title":"MINDY1 Is a Downstream Target of the Polyamines and Promotes Embryonic Stem Cell Self-Renewal","volume":"36","author":"James","year":"2018","journal-title":"Stem Cells"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"339","DOI":"10.1007\/978-1-61779-034-8_21","article-title":"Polyamine transport systems in mammalian cells and tissues","volume":"720","author":"Uemura","year":"2011","journal-title":"Methods Mol. Biol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"449","DOI":"10.1007\/s00417-020-04900-8","article-title":"Hypoxic stress increases NF-\u03baB and iNOS mRNA expression in normal, but not in keratoconus corneal fibroblasts","volume":"259","author":"Stachon","year":"2021","journal-title":"Graefe\u2019s Arch. Clin. Exp. Ophthalmol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"6228","DOI":"10.1038\/onc.2008.300","article-title":"NF-\u03baB signaling, liver disease and hepatoprotective agents","volume":"27","author":"Sun","year":"2008","journal-title":"Oncogene"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"108","DOI":"10.1038\/nrgastro.2010.213","article-title":"NF-\u03baB in the liver\u2014linking injury, fibrosis and hepatocellular carcinoma","volume":"8","author":"Luedde","year":"2011","journal-title":"Nat. Rev. Gastroenterol. Hepatol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"4843","DOI":"10.1073\/pnas.0735876100","article-title":"Translational control of inducible nitric oxide synthase expression by arginine can explain the arginine paradox","volume":"100","author":"Lee","year":"2003","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"1764","DOI":"10.4161\/cc.10.11.15828","article-title":"Hepatic stellate cells and astrocytes: Stars of scar formation and tissue repair","volume":"10","author":"Schachtrup","year":"2011","journal-title":"Cell Cycle"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"373","DOI":"10.1016\/S0197-0186(00)00116-9","article-title":"Acquisition of blood-tissue barrier-supporting features by hepatic stellate cells and astrocytes of myofibroblastic phenotype. Inverse dynamics of metallothionein and glial fibrillary acidic protein expression","volume":"38","author":"Buniatian","year":"2001","journal-title":"Neurochem. Int."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"6561","DOI":"10.1016\/j.febslet.2006.11.003","article-title":"Arginase-flotillin interaction brings arginase to red blood cell membrane","volume":"580","author":"Jiang","year":"2006","journal-title":"FEBS Lett."},{"key":"ref_52","first-page":"90","article-title":"To Give or Not to Give? Lessons from the Arginine Paradox","volume":"4","author":"Dioguardi","year":"2011","journal-title":"J. Nutrigenet. Nutrigenom."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"2752S","DOI":"10.1093\/jn\/134.10.2752S","article-title":"Plasma membrane transporters for arginine","volume":"134","author":"Closs","year":"2004","journal-title":"J. Nutr."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"24017","DOI":"10.1074\/jbc.271.39.24017","article-title":"Induced nitric oxide synthesis is dependent on induced alternatively spliced CAT-2 encoding L-arginine transport in brain astrocytes","volume":"271","author":"Stevens","year":"1996","journal-title":"J. Biol. Chem."}],"container-title":["Cells"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2073-4409\/11\/3\/508\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T22:12:45Z","timestamp":1760134365000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2073-4409\/11\/3\/508"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,2,1]]},"references-count":54,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2022,2]]}},"alternative-id":["cells11030508"],"URL":"https:\/\/doi.org\/10.3390\/cells11030508","relation":{},"ISSN":["2073-4409"],"issn-type":[{"value":"2073-4409","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,2,1]]}}}