{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T01:05:41Z","timestamp":1791335141278,"version":"4.3.1"},"reference-count":5,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2022,3,4]],"date-time":"2022-03-04T00:00:00Z","timestamp":1646352000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001807","name":"S\u00e3o Paulo Research Foundation","doi-asserted-by":"publisher","award":["2019\/07230-8"],"award-info":[{"award-number":["2019\/07230-8"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"S\u00e3o Paulo Research Foundation","doi-asserted-by":"publisher","award":["2020\/14610-9"],"award-info":[{"award-number":["2020\/14610-9"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"S\u00e3o Paulo Research Foundation","doi-asserted-by":"publisher","award":["2021\/00141-0"],"award-info":[{"award-number":["2021\/00141-0"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfeicoamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","award":["88887.647796\/2021-00"],"award-info":[{"award-number":["88887.647796\/2021-00"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"National Council for Scientific and Technological Development","doi-asserted-by":"publisher","award":["308504\/2021-6"],"award-info":[{"award-number":["308504\/2021-6"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"National Council for Scientific and Technological Development","doi-asserted-by":"publisher","award":["133901\/2021.1"],"award-info":[{"award-number":["133901\/2021.1"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells"],"abstract":"<jats:p>Background: Low bioavailability of nitric oxide (NO) is related to the pathophysiology of preeclampsia (PE). In the present study, we investigated the effect of nebivolol (NEB), a \u03b23-receptor agonist with vasodilator properties, on the NO synthesis in endothelial cells incubated with plasma from preeclamptic patients. Methods and results: Human umbilical vein endothelial cells (HUVECs) were incubated with plasma from healthy pregnant (HP) and PE women; NO quantification was assessed by a fluorescence compound. We found that endothelial cells incubated with plasma from women with PE show lower NO levels compared with the HP group (p &lt; 0.0001). However, NEB treatment increases NO levels, partially, mediated by \u03b23 adrenergic receptors (p &lt; 0.0001) and through eNOS activation (p &lt; 0.0001). Conclusions: Our results suggest that NEB acts in NO synthesis through eNOS activation and \u03b23 adrenergic receptors in the endothelium. However, further studies will be needed to understand this molecule.<\/jats:p>","DOI":"10.3390\/cells11050883","type":"journal-article","created":{"date-parts":[[2022,3,6]],"date-time":"2022-03-06T20:35:50Z","timestamp":1646598950000},"page":"883","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Nebivolol Increases Nitric Oxide Synthase via \u03b23 Adrenergic Receptor in Endothelial Cells Following Exposure to Plasma from Preeclamptic Patients"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4257-3822","authenticated-orcid":false,"given":"Thaina Omia","family":"Bueno-Pereira","sequence":"first","affiliation":[{"name":"Department of Biophysics and Pharmacology, Institute of Biosciences of Botucatu, Universidade Estadual Paulista (UNESP), Distrito Rubiao Junior, Botucatu 18680-000, Sao Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8041-5753","authenticated-orcid":false,"given":"Priscila Rezeck","family":"Nunes","sequence":"additional","affiliation":[{"name":"Department of Biophysics and Pharmacology, Institute of Biosciences of Botucatu, Universidade Estadual Paulista (UNESP), Distrito Rubiao Junior, Botucatu 18680-000, Sao Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6085-8821","authenticated-orcid":false,"given":"Mariana Bertozzi","family":"Matheus","sequence":"additional","affiliation":[{"name":"Department of Biophysics and Pharmacology, Institute of Biosciences of Botucatu, Universidade Estadual Paulista (UNESP), Distrito Rubiao Junior, Botucatu 18680-000, Sao Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ananda Lini","family":"Vieira da Rocha","sequence":"additional","affiliation":[{"name":"Department of Biophysics and Pharmacology, Institute of Biosciences of Botucatu, Universidade Estadual Paulista (UNESP), Distrito Rubiao Junior, Botucatu 18680-000, Sao Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valeria Cristina","family":"Sandrim","sequence":"additional","affiliation":[{"name":"Department of Biophysics and Pharmacology, Institute of Biosciences of Botucatu, Universidade Estadual Paulista (UNESP), Distrito Rubiao Junior, Botucatu 18680-000, Sao Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,3,4]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1038\/sj.jhh.1000571","article-title":"Nebivolol vs atenolol and placebo in essential hypertension: A double-blind randomised trial","volume":"12","author":"Taylor","year":"1998","journal-title":"J. Hum. Hypertens."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1198","DOI":"10.1161\/CIRCULATIONAHA.104.532960","article-title":"Endothelial \u03b23-adrenoreceptors mediate nitric oxide-dependent vasorelaxation of coronary microvessels in response to the third-generation \u03b2-blocker nebivolol","volume":"112","author":"Dessy","year":"2005","journal-title":"Circulation"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1198","DOI":"10.1111\/jcmm.12767","article-title":"Plasma from pre-eclamptic patients induces the expression of the anti-angiogenic miR-195-5p in endothelial cells","volume":"20","author":"Sandrim","year":"2016","journal-title":"J. Cell. Mol. Med."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"110659","DOI":"10.1016\/j.biopha.2020.110659","article-title":"SIRT1-dependent effects of resveratrol and grape juice in an in vitro model of preeclampsia","volume":"131","author":"Cinegaglia","year":"2020","journal-title":"Biomed. Pharmacother."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"ACOG (2020). ACOG Practice Bulletin Clinical Management Guidelines for Obstetrician Gynecologists. Gestational Hypertension and Preeclampsia. Obstet. Gynecol., 135, e237\u2013e260.","DOI":"10.1097\/AOG.0000000000003891"}],"container-title":["Cells"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2073-4409\/11\/5\/883\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T22:31:47Z","timestamp":1760135507000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2073-4409\/11\/5\/883"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,3,4]]},"references-count":5,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2022,3]]}},"alternative-id":["cells11050883"],"URL":"https:\/\/doi.org\/10.3390\/cells11050883","relation":{},"ISSN":["2073-4409"],"issn-type":[{"value":"2073-4409","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,3,4]]}}}