{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T16:09:52Z","timestamp":1792080592446,"version":"4.4.0"},"reference-count":32,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2022,3,14]],"date-time":"2022-03-14T00:00:00Z","timestamp":1647216000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001665","name":"Agence Nationale de la Recherche","doi-asserted-by":"publisher","award":["ANR-10-IBHU-004"],"award-info":[{"award-number":["ANR-10-IBHU-004"]}],"id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001665","name":"Agence Nationale de la Recherche","doi-asserted-by":"publisher","award":["ANR-16-RHUS-0009"],"award-info":[{"award-number":["ANR-16-RHUS-0009"]}],"id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001674","name":"Fondation Leducq","doi-asserted-by":"publisher","award":["16 CVD 04"],"award-info":[{"award-number":["16 CVD 04"]}],"id":[{"id":"10.13039\/501100001674","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001677","name":"Inserm","doi-asserted-by":"publisher","award":["annual funding of the CarMeN Laboratory"],"award-info":[{"award-number":["annual funding of the CarMeN Laboratory"]}],"id":[{"id":"10.13039\/501100001677","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006687","name":"Claude Bernard University Lyon 1","doi-asserted-by":"publisher","award":["annual funding of the CarMeN Laboratory"],"award-info":[{"award-number":["annual funding of the CarMeN Laboratory"]}],"id":[{"id":"10.13039\/501100006687","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Agence R\u00e9gionale de Sant\u00e9 Auvergne-Rhone-Alpes","award":["Ann\u00e9e-recherche"],"award-info":[{"award-number":["Ann\u00e9e-recherche"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells"],"abstract":"<jats:p>Hypothermia provides an effective neuro and cardio-protection in clinical settings implying ischemia\/reperfusion injury (I\/R). At the onset of reperfusion, succinate-induced reactive oxygen species (ROS) production, impaired oxidative phosphorylation (OXPHOS), and decreased Ca2+ retention capacity (CRC) concur to mitochondrial damages. We explored the effects of temperature from 6 to 37 \u00b0C on OXPHOS, ROS production, and CRC, using isolated mitochondria from mouse brain and heart. Oxygen consumption and ROS production was gradually inhibited when cooling from 37 to 6 \u00b0C in brain mitochondria (BM) and heart mitochondria (HM). The decrease in ROS production was gradual in BM but steeper between 31 and 20 \u00b0C in HM. In respiring mitochondria, the gradual activation of complex II, in addition of complex I, dramatically enhanced ROS production at all temperatures without modifying respiration, likely because of ubiquinone over-reduction. Finally, CRC values were linearly increased by cooling in both BM and HM. In BM, the Ca2+ uptake rate by the mitochondrial calcium uniporter (MCU) decreased by 2.7-fold between 25 and 37 \u00b0C, but decreased by 5.7-fold between 25 and 37 \u00b0C in HM. In conclusion, mild cold (25\u201337 \u00b0C) exerts differential inhibitory effects by preventing ROS production, by reverse electron transfer (RET) in BM, and by reducing MCU-mediated Ca2+ uptake rate in BM and HM.<\/jats:p>","DOI":"10.3390\/cells11060989","type":"journal-article","created":{"date-parts":[[2022,3,15]],"date-time":"2022-03-15T03:06:10Z","timestamp":1647313570000},"page":"989","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Cooling Uncouples Differentially ROS Production from Respiration and Ca2+ Homeostasis Dynamic in Brain and Heart Mitochondria"],"prefix":"10.3390","volume":"11","author":[{"given":"Neven","family":"Stevic","sequence":"first","affiliation":[{"name":"Univ-Lyon, CarMeN Laboratory, Inserm U1060, Universit\u00e9 Claude Bernard Lyon 1, INSA Lyon, F-69550 Bron, France"},{"name":"Hospices Civils de Lyon, Groupement Hospitalier EST, D\u00e9partement de Cardiologie, IHU-OPERA B\u00e2timent B13, F-69500 Bron, France"},{"name":"Hospices Civils de Lyon, H\u00f4pital Edouard Herriot, Service de M\u00e9decine Intensive-R\u00e9animation, F-69437 Lyon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer","family":"Maalouf","sequence":"additional","affiliation":[{"name":"Univ-Lyon, CarMeN Laboratory, Inserm U1060, Universit\u00e9 Claude Bernard Lyon 1, INSA Lyon, F-69550 Bron, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laurent","family":"Argaud","sequence":"additional","affiliation":[{"name":"Univ-Lyon, CarMeN Laboratory, Inserm U1060, Universit\u00e9 Claude Bernard Lyon 1, INSA Lyon, F-69550 Bron, France"},{"name":"Hospices Civils de Lyon, H\u00f4pital Edouard Herriot, Service de M\u00e9decine Intensive-R\u00e9animation, F-69437 Lyon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"No\u00eblle","family":"Gallo-Bona","sequence":"additional","affiliation":[{"name":"Univ-Lyon, CarMeN 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France"},{"name":"Hospices Civils de Lyon, Groupement Hospitalier EST, D\u00e9partement de Cardiologie, IHU-OPERA B\u00e2timent B13, F-69500 Bron, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ludovic","family":"Gomez","sequence":"additional","affiliation":[{"name":"Univ-Lyon, CarMeN Laboratory, Inserm U1060, Universit\u00e9 Claude Bernard Lyon 1, INSA Lyon, F-69550 Bron, France"},{"name":"Hospices Civils de Lyon, Groupement Hospitalier EST, D\u00e9partement de Cardiologie, IHU-OPERA B\u00e2timent B13, F-69500 Bron, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claire","family":"Crola Da Silva","sequence":"additional","affiliation":[{"name":"Univ-Lyon, CarMeN Laboratory, Inserm U1060, Universit\u00e9 Claude Bernard Lyon 1, INSA Lyon, F-69550 Bron, France"},{"name":"Hospices Civils de Lyon, Groupement Hospitalier EST, D\u00e9partement de Cardiologie, IHU-OPERA B\u00e2timent B13, F-69500 Bron, 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