{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T08:26:44Z","timestamp":1790843204229,"version":"4.1.0"},"reference-count":37,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2022,3,29]],"date-time":"2022-03-29T00:00:00Z","timestamp":1648512000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells"],"abstract":"<jats:p>Chronic wounds, such as leg ulcers associated with sickle cell disease, occur as a consequence of a prolonged inflammatory phase during the healing process. They are extremely hard to heal and persist as a significant health care problem due to the absence of effective treatment and the uprising number of patients. Indeed, there is a critical need to develop novel cell- and tissue-based therapies to treat these chronic wounds. Development in skin engineering leads to a small catalogue of available substitutes manufactured in Good Manufacturing Practices compliant (GMPc) conditions. Those substitutes are produced using primary cells that could limit their use due to restricted sourcing. Here, we propose GMPc protocols to produce functional populations of keratinocytes and fibroblasts derived from pluripotent stem cells to reconstruct the associated dermo-epidermal substitute with plasma-based fibrin matrix. In addition, this manufactured composite skin is biologically active and enhances in vitro wounding of keratinocytes. The proposed composite skin opens new perspectives for skin replacement using allogeneic substitute.<\/jats:p>","DOI":"10.3390\/cells11071151","type":"journal-article","created":{"date-parts":[[2022,3,29]],"date-time":"2022-03-29T21:40:14Z","timestamp":1648590014000},"page":"1151","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Clinical Grade Human Pluripotent Stem Cell-Derived Engineered Skin Substitutes Promote Keratinocytes Wound Closure In Vitro"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7968-3304","authenticated-orcid":false,"given":"Sophie","family":"Domingues","sequence":"first","affiliation":[{"name":"Centre d\u2019Etude des Cellules Souches, 91100 Corbeil-Essonnes, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9350-654X","authenticated-orcid":false,"given":"Annabelle","family":"Darle","sequence":"additional","affiliation":[{"name":"Centre d\u2019Etude des Cellules Souches, 91100 Corbeil-Essonnes, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1264-587X","authenticated-orcid":false,"given":"Yolande","family":"Masson","sequence":"additional","affiliation":[{"name":"Centre d\u2019Etude des Cellules Souches, 91100 Corbeil-Essonnes, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manoubia","family":"Saidani","sequence":"additional","affiliation":[{"name":"Centre d\u2019Etude des Cellules Souches, 91100 Corbeil-Essonnes, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emilie","family":"Lagoutte","sequence":"additional","affiliation":[{"name":"URGO RID, 42 Rue de Longvic, 21300 Chen\u00f4ve, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana","family":"Bejanariu","sequence":"additional","affiliation":[{"name":"Centre d\u2019Etude des Cellules Souches, 91100 Corbeil-Essonnes, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2407-5387","authenticated-orcid":false,"given":"Julien","family":"Coutier","sequence":"additional","affiliation":[{"name":"Centre d\u2019Etude des Cellules Souches, 91100 Corbeil-Essonnes, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raif Eren","family":"Ayata","sequence":"additional","affiliation":[{"name":"INSERM U861, I-Stem, AFM, Institute for Stem Cell Therapy and Exploration of Monogenic Diseases, 91100 Corbeil-Essonnes, France"},{"name":"Universit\u00e9 Paris-Saclay, Universit\u00e9 d\u2019Evry, U861, 91100 Corbeil-Essonnes, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marielle","family":"Bouschbacher","sequence":"additional","affiliation":[{"name":"URGO RID, 42 Rue de Longvic, 21300 Chen\u00f4ve, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marc","family":"Peschanski","sequence":"additional","affiliation":[{"name":"Centre d\u2019Etude des Cellules Souches, 91100 Corbeil-Essonnes, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0898-1582","authenticated-orcid":false,"given":"Gilles","family":"Lemaitre","sequence":"additional","affiliation":[{"name":"INSERM U861, I-Stem, AFM, Institute for Stem Cell Therapy and Exploration of Monogenic Diseases, 91100 Corbeil-Essonnes, France"},{"name":"Universit\u00e9 Paris-Saclay, Universit\u00e9 d\u2019Evry, U861, 91100 Corbeil-Essonnes, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christine","family":"Baldeschi","sequence":"additional","affiliation":[{"name":"INSERM U861, I-Stem, AFM, Institute for Stem Cell Therapy and Exploration of Monogenic Diseases, 91100 Corbeil-Essonnes, France"},{"name":"Universit\u00e9 Paris-Saclay, Universit\u00e9 d\u2019Evry, U861, 91100 Corbeil-Essonnes, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,3,29]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"118932","DOI":"10.1016\/j.lfs.2020.118932","article-title":"Stem Cells and Growth Factors-Based Delivery Approaches for Chronic Wound Repair and Regeneration: A Promise to heal from within","volume":"268","author":"Sharma","year":"2021","journal-title":"Life Sci."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"17","DOI":"10.4049\/jimmunol.1700223","article-title":"Macrophage-Mediated Inflammation in Normal and Diabetic Wound Healing","volume":"199","author":"Boniakowski","year":"2017","journal-title":"J. Immunol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1186\/s13287-019-1212-2","article-title":"Skin Tissue Engineering: Wound Healing Based on Stem-Cell-Based Therapeutic Strategies","volume":"10","author":"Chehelgerdi","year":"2019","journal-title":"Stem Cell Res. Ther."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"348","DOI":"10.1089\/wound.2018.0918","article-title":"Leg Ulcers in Sickle-Cell Disease: Treatment Update","volume":"9","author":"Monfort","year":"2020","journal-title":"Adv. Wound Care"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"114","DOI":"10.1111\/wrr.12683","article-title":"The Humanistic and Economic Burden of Chronic Wounds: A Systematic Review","volume":"27","author":"Olsson","year":"2019","journal-title":"Wound Repair Regen."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"599","DOI":"10.1007\/s12325-017-0478-y","article-title":"Chronic Wound Healing: A Review of Current Management and Treatments","volume":"34","author":"Han","year":"2017","journal-title":"Adv. Ther."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"639","DOI":"10.1080\/09546634.2018.1530443","article-title":"Skin Substitutes for Acute and Chronic Wound Healing: An Updated Review","volume":"31","author":"Dai","year":"2020","journal-title":"J. Dermatol. Treat."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"216","DOI":"10.1159\/000381877","article-title":"Stem Cells in Wound Healing: The Future of Regenerative Medicine? A Mini-Review","volume":"62","author":"Duscher","year":"2016","journal-title":"Gerontology"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"25476","DOI":"10.3390\/ijms161025476","article-title":"Stem Cells in Skin Regeneration, Wound Healing, and Their Clinical Applications","volume":"16","author":"Ojeh","year":"2015","journal-title":"Int. J. Mol. Sci."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"20140367","DOI":"10.1098\/rstb.2014.0367","article-title":"Present and Future Challenges of Induced Pluripotent Stem Cells","volume":"370","author":"Ohnuki","year":"2015","journal-title":"Philos. Trans. R. Soc. B Biol. Sci."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1745","DOI":"10.1016\/S0140-6736(09)61496-3","article-title":"Human Embryonic Stem-Cell Derivatives for Full Reconstruction of the Pluristratified Epidermis: A Preclinical Study","volume":"374","author":"Guenou","year":"2009","journal-title":"Lancet"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"8797","DOI":"10.1073\/pnas.1100332108","article-title":"Generation of Keratinocytes from Normal and Recessive Dystrophic Epidermolysis Bullosa-Induced Pluripotent Stem Cells","volume":"108","author":"Itoh","year":"2011","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1089\/cell.2020.0046","article-title":"Generation of Keratinocytes from Human Induced Pluripotent Stem Cells Under Defined Culture Conditions","volume":"23","author":"Sah","year":"2021","journal-title":"Cell. Reprogramming"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1186\/scrt51","article-title":"Fibroblasts Derived from Human Embryonic Stem Cells Direct Development and Repair of 3D Human Skin Equivalents","volume":"2","author":"Shamis","year":"2011","journal-title":"Stem Cell Res. Ther."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Itoh, M., Umegaki-Arao, N., Guo, Z., Liu, L., Higgins, C.A., and Christiano, A.M. (2013). Generation of 3D Skin Equivalents Fully Reconstituted from Human Induced Pluripotent Stem Cells (IPSCs). PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0077673"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1935","DOI":"10.3389\/fimmu.2017.01935","article-title":"Directed Differentiation of Human Induced Pluripotent Stem Cells into Dendritic Cells Displaying Tolerogenic Properties and Resembling the CD141 + Subset","volume":"8","author":"Sachamitr","year":"2017","journal-title":"Front. Immunol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"546","DOI":"10.1016\/j.stemcr.2020.06.014","article-title":"A Manually Curated Database on Clinical Studies Involving Cell Products Derived from Human Pluripotent Stem Cells","volume":"15","author":"Kobold","year":"2020","journal-title":"Stem Cell Rep."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"379","DOI":"10.1016\/j.scr.2016.08.011","article-title":"Development and Production of Good Manufacturing Practice Grade Human Embryonic Stem Cell Lines as Source Material for Clinical Application","volume":"17","author":"Downie","year":"2016","journal-title":"Stem Cell Res."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Cavallero, S., Neves Granito, R., Stockholm, D., Azzolin, P., Martin, M.T., and Fortunel, N.O. (2020). Exposure of Human Skin Organoids to Low Genotoxic Stress Can Promote Epithelial-to-Mesenchymal Transition in Regenerating Keratinocyte Precursor Cells. Cells, 9.","DOI":"10.3390\/cells9081912"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1186\/s13287-018-0958-2","article-title":"Establishment of a Complex Skin Structure via Layered Co-Culture of Keratinocytes and Fibroblasts Derived from Induced Pluripotent Stem Cells","volume":"9","author":"Kim","year":"2018","journal-title":"Stem Cell Res. Ther."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"17361","DOI":"10.1073\/pnas.1908180116","article-title":"Single-Cell RNA-Seq Identifies a Reversible Mesodermal Activation in Abnormally Specified Epithelia of P63 EEC Syndrome","volume":"116","author":"Soares","year":"2019","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"552","DOI":"10.1161\/CIRCRESAHA.119.315491","article-title":"Generation of Quiescent Cardiac Fibroblasts From Human Induced Pluripotent Stem Cells for In Vitro Modeling of Cardiac Fibrosis","volume":"125","author":"Zhang","year":"2019","journal-title":"Circ. Res."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"868","DOI":"10.1097\/00007890-199909270-00021","article-title":"The Control of Epidermal Stem Cells (Holoclones) in the Treatment of Massive Full-Thickness Burns with Autologous Keratinocytes Cultured on Fibrin","volume":"68","author":"Pellegrini","year":"1999","journal-title":"Transplantation"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1588","DOI":"10.1097\/00007890-200012150-00009","article-title":"Long-Term Regeneration of Human Epidermis on Third Degree Burns Transplanted with Autologous Cultured Epithelium Grown on a Fibrin Matrix","volume":"70","author":"Ronfard","year":"2000","journal-title":"Transplantation"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"2565","DOI":"10.1038\/jid.2012.192","article-title":"Different Gene Expression Patterns in Human Papillary and Reticular Fibroblasts","volume":"132","author":"Janson","year":"2012","journal-title":"J. Investig. Dermatol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"483","DOI":"10.1016\/j.ejcb.2015.08.001","article-title":"Fibroblast Heterogeneity and Its Implications for Engineering Organotypic Skin Models in Vitro","volume":"94","author":"Sriram","year":"2015","journal-title":"Eur. J. Cell Biol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1007\/s10561-011-9247-3","article-title":"Feeder Layer- and Animal Product-Free Culture of Neonatal Foreskin Keratinocytes: Improved Performance, Usability, Quality and Safety","volume":"13","author":"Verween","year":"2012","journal-title":"Cell Tissue Bank."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"279","DOI":"10.20471\/acc.2016.55.02.15","article-title":"Fibrin Gel as a Scaffold for Skin Substitute\u2013Production and Clinical Experience","volume":"55","author":"Kljenak","year":"2016","journal-title":"Acta Clin. Croat."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"350","DOI":"10.1097\/01.TP.0000112381.80964.85","article-title":"Human Plasma as a Dermal Scaffold for the Generation of a Completely Autologous Bioengineered Skin","volume":"77","author":"Llames","year":"2004","journal-title":"Transplantation"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"372","DOI":"10.1016\/j.jbiotec.2005.12.021","article-title":"Culture of Skin Cells in 3D Rather than 2D Improves Their Ability to Survive Exposure to Cytotoxic Agents","volume":"122","author":"Sun","year":"2006","journal-title":"J. Biotechnol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"731","DOI":"10.1016\/j.ejcb.2006.12.005","article-title":"Effects of Fibroblasts and Microenvironment on Epidermal Regeneration and Tissue Function in Long-Term Skin Equivalents","volume":"86","author":"Boehnke","year":"2007","journal-title":"Eur. J. Cell Biol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"845","DOI":"10.1111\/j.1525-1594.2005.00138.x","article-title":"A Study of Cytokines Released from Fibroblasts in Cultured Dermal Substitute","volume":"29","author":"Kubo","year":"2005","journal-title":"Artif. Organs"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"444","DOI":"10.1046\/j.1523-1747.2000.00869.x","article-title":"Normal Growth and Differentiation in a Spontaneously Immortalized Near-Diploid Human Keratinocyte Cell Line, NIKS","volume":"114","author":"Schlosser","year":"2000","journal-title":"J. Investig. Dermatol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"523","DOI":"10.1016\/j.stem.2020.09.014","article-title":"Pluripotent Stem Cell-Based Cell Therapy-Promise and Challenges","volume":"27","author":"Yamanaka","year":"2020","journal-title":"Cell Stem Cell"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"895","DOI":"10.1002\/stem.636","article-title":"Concise Review: Epidermal Grafting: The Case for Pluripotent Stem Cells","volume":"29","author":"Nissan","year":"2011","journal-title":"Stem Cells"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"102035","DOI":"10.1016\/j.scr.2020.102035","article-title":"Haplobanking Induced Pluripotent Stem Cells for Clinical Use","volume":"49","author":"Sullivan","year":"2020","journal-title":"Stem Cell Res."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"102451","DOI":"10.1016\/j.scr.2021.102451","article-title":"Derivation of Induced Pluripotent Stem Cells Line (RCPCMi007-A-1) with Inactivation of the Beta-2-Microglobulin Gene by CRISPR\/Cas9 Genome Editing","volume":"55","author":"Bogomiakova","year":"2021","journal-title":"Stem Cell Res."}],"container-title":["Cells"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2073-4409\/11\/7\/1151\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T22:45:42Z","timestamp":1760136342000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2073-4409\/11\/7\/1151"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,3,29]]},"references-count":37,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2022,4]]}},"alternative-id":["cells11071151"],"URL":"https:\/\/doi.org\/10.3390\/cells11071151","relation":{},"ISSN":["2073-4409"],"issn-type":[{"value":"2073-4409","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,3,29]]}}}