{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T04:36:39Z","timestamp":1787027799574,"version":"build-2736575974"},"reference-count":24,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2022,4,8]],"date-time":"2022-04-08T00:00:00Z","timestamp":1649376000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004569","name":"Ministry of Science and Higher Education","doi-asserted-by":"publisher","award":["DI2016 012746"],"award-info":[{"award-number":["DI2016 012746"]}],"id":[{"id":"10.13039\/501100004569","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100017600","name":"National Research Institute of Animal Production","doi-asserted-by":"publisher","award":["04-19-11-21"],"award-info":[{"award-number":["04-19-11-21"]}],"id":[{"id":"10.13039\/100017600","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells"],"abstract":"<jats:p>Matrix metalloproteinases (MMPs) represent a family of enzymes capable of biocatalytically breaking down the structural and functional proteins responsible for extracellular matrix (ECM) integrity. This capability is widely used in physiological processes; however, imbalanced MMP activity can trigger the onset and progression of various pathological changes, including the neoplasmic transformation of different cell types. We sought to uncover molecular mechanisms underlying alterations in transcriptional profiles of genes coding for MMPs, which were comprehensively identified in equine adult dermal tissue bioptates, sarcoid-derived explants, and ex vivo expanded adult cutaneous fibroblast cell (ACFC) lines subjected to inducible oncogenic transformation into sarcoid-like cells. The results strongly support the hypothesis that the transcriptional activity of MMP genes correlates with molecular modifications arising in equine dermal cells during their conversion into sarcoid cells. The alterations in MMP transcription signatures occurs in both sarcoid tissues and experimentally transformed equine ACFC lines expressing BPV1-E4^E1 transgene, which were characterized by gene up- and down-regulation patterns.<\/jats:p>","DOI":"10.3390\/cells11081268","type":"journal-article","created":{"date-parts":[[2022,4,8]],"date-time":"2022-04-08T12:11:14Z","timestamp":1649419874000},"page":"1268","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Assessment of BPV-1 Mediated Matrix Metalloproteinase Genes Deregulation in the In Vivo and In Vitro Models Designed to Explore Molecular Nature of Equine Sarcoids"],"prefix":"10.3390","volume":"11","author":[{"given":"Przemys\u0142aw","family":"Podstawski","sequence":"first","affiliation":[{"name":"Department of Animal Molecular Biology, National Research Institute of Animal Production, Krakowska 1 Street, 32-083 Balice, Poland"},{"name":"Department of Animal Reproduction, Anatomy and Genomics, University of Agriculture in Krak\u00f3w, Mickiewicza 24\/28, 30-059 Krakow, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8555-0495","authenticated-orcid":false,"given":"Katarzyna","family":"Ropka-Molik","sequence":"additional","affiliation":[{"name":"Department of Animal Molecular Biology, National Research Institute of Animal Production, Krakowska 1 Street, 32-083 Balice, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4667-5495","authenticated-orcid":false,"given":"Ewelina","family":"Semik-Gurgul","sequence":"additional","affiliation":[{"name":"Department of Animal Molecular Biology, National Research Institute of Animal Production, Krakowska 1 Street, 32-083 Balice, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4060-1893","authenticated-orcid":false,"given":"Marcin","family":"Samiec","sequence":"additional","affiliation":[{"name":"Department of Reproductive Biotechnology and Cryoconservation, National Research Institute of Animal Production, Krakowska 1 Street, 32-083 Balice, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0068-6407","authenticated-orcid":false,"given":"Maria","family":"Skrzyszowska","sequence":"additional","affiliation":[{"name":"Department of Reproductive Biotechnology and Cryoconservation, National Research Institute of Animal Production, Krakowska 1 Street, 32-083 Balice, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zenon","family":"Podstawski","sequence":"additional","affiliation":[{"name":"Department of Animal Reproduction, Anatomy and Genomics, University of Agriculture in Krak\u00f3w, Mickiewicza 24\/28, 30-059 Krakow, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomasz","family":"Szmato\u0142a","sequence":"additional","affiliation":[{"name":"Department of Animal Molecular Biology, National Research Institute of Animal Production, Krakowska 1 Street, 32-083 Balice, Poland"},{"name":"Center for Experimental and Innovative Medicine, University of Agriculture in Krakow, R\u0119dzina 1c Street, 30-248 Krakow, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maciej","family":"Witkowski","sequence":"additional","affiliation":[{"name":"Institute of Veterinary Medicine, University Centre of Veterinary Medicine JU-AU, Mickiewicza 24\/28, 30-248 Krakow, Poland"},{"name":"Szpital Koni S\u0142u\u017cewiec, Pu\u0142awska 266, 02-684 Warszawa, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3926-9595","authenticated-orcid":false,"given":"Klaudia","family":"Pawlina-Tyszko","sequence":"additional","affiliation":[{"name":"Department of Animal Molecular Biology, National Research Institute of Animal Production, Krakowska 1 Street, 32-083 Balice, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,4,8]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/bs.pmbts.2017.02.005","article-title":"Biochemical and Biological Attributes of Matrix Metalloproteinases","volume":"147","author":"Cui","year":"2017","journal-title":"Prog. 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