{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,9]],"date-time":"2026-05-09T15:18:34Z","timestamp":1778339914878,"version":"3.51.4"},"reference-count":74,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2022,4,10]],"date-time":"2022-04-10T00:00:00Z","timestamp":1649548800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003407","name":"MIUR","doi-asserted-by":"publisher","award":["PRIN, Prot. 20152CB22L"],"award-info":[{"award-number":["PRIN, Prot. 20152CB22L"]}],"id":[{"id":"10.13039\/501100003407","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Fondazione Di Bella","award":["no grant number"],"award-info":[{"award-number":["no grant number"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells"],"abstract":"<jats:p>Oxygen is important for lipid metabolism, being involved in both enzymatic transformations and oxidative reactivity, and is particularly influent when genetic diseases impair the repair machinery of the cells, such as described for Cockayne syndrome (CS). We used two cellular models of transformed fibroblasts defective for CSA and CSB genes and their normal counterparts, grown for 24 h under various oxygen tensions (hyperoxic 21%, physioxic 5% and hypoxic 1%) to examine the fatty acid-based membrane remodeling by GC analysis of fatty acid methyl esters derived from membrane phospholipids. Overall, we first distinguished differences due to oxygen tensions: (a) hyperoxia induced a general boost of desaturase enzymatic activity in both normal and defective CSA and CSB cell lines, increasing monounsaturated fatty acids (MUFA), whereas polyunsaturated fatty acids (PUFA) did not undergo oxidative consumption; (b) hypoxia slowed down desaturase activities, mostly in CSA cell lines and defective CSB, causing saturated fatty acids (SFA) to increase, whereas PUFA levels diminished, suggesting their involvement in hypoxia-related signaling. CSB-deprived cells are the most sensitive to oxidation and CSA-deprived cells are the most sensitive to the radical-based formation of trans fatty acids (TFA). The results point to the need to finely differentiate biological targets connected to genetic impairments and, consequently, suggest the better definition of cell protection and treatments through accurate molecular profiling that includes membrane lipidomes.<\/jats:p>","DOI":"10.3390\/cells11081286","type":"journal-article","created":{"date-parts":[[2022,4,10]],"date-time":"2022-04-10T06:02:54Z","timestamp":1649570574000},"page":"1286","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Effects of Oxygen Tension for Membrane Lipidome Remodeling of Cockayne Syndrome Cell Models"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1359-0869","authenticated-orcid":false,"given":"Carla","family":"Ferreri","sequence":"first","affiliation":[{"name":"Istituto per la Sintesi Organica e la Fotoreattivit\u00e0, Consiglio Nazionale delle Ricerche, Via P. Gobetti 101, 40129 Bologna, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0865-4861","authenticated-orcid":false,"given":"Anna","family":"Sansone","sequence":"additional","affiliation":[{"name":"Istituto per la Sintesi Organica e la Fotoreattivit\u00e0, Consiglio Nazionale delle Ricerche, Via P. Gobetti 101, 40129 Bologna, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marios G.","family":"Krokidis","sequence":"additional","affiliation":[{"name":"Institute of Nanoscience and Nanotechnology, N.C.S.R. \u201cDemokritos\u201d, Agia Paraskevi Attikis, Athens 15310, Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7163-3978","authenticated-orcid":false,"given":"Annalisa","family":"Masi","sequence":"additional","affiliation":[{"name":"Istituto per la Sintesi Organica e la Fotoreattivit\u00e0, Consiglio Nazionale delle Ricerche, Via P. Gobetti 101, 40129 Bologna, Italy"},{"name":"Institute of Crystallography, Consiglio Nazionale delle Ricerche, Monterotondo Stazione, 00015 Rome, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8383-8751","authenticated-orcid":false,"given":"Barbara","family":"Pascucci","sequence":"additional","affiliation":[{"name":"Institute of Crystallography, Consiglio Nazionale delle Ricerche, Monterotondo Stazione, 00015 Rome, Italy"},{"name":"Department of Environment and Health, Istituto Superiore di Sanit\u00e0, Viale Regina Elena 299, 00161 Rome, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mariarosaria","family":"D\u2019Errico","sequence":"additional","affiliation":[{"name":"Department of Environment and Health, Istituto Superiore di Sanit\u00e0, Viale Regina Elena 299, 00161 Rome, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2626-2925","authenticated-orcid":false,"given":"Chryssostomos","family":"Chatgilialoglu","sequence":"additional","affiliation":[{"name":"Istituto per la Sintesi Organica e la Fotoreattivit\u00e0, Consiglio Nazionale delle Ricerche, Via P. Gobetti 101, 40129 Bologna, Italy"},{"name":"Center for Advanced Technologies, Adam Mickiewicz University, 61-614 Pozna\u0144, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,4,10]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1146\/annurev-physiol-020518-114444","article-title":"Phospholipid Remodeling in Physiology and Disease","volume":"81","author":"Wang","year":"2019","journal-title":"Ann. Rev. Physiol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"883","DOI":"10.1016\/S0021-9258(18)70453-5","article-title":"Metabolism of glycerolipides: A comparison of lecithin and triglyceride synthesis","volume":"231","author":"Lands","year":"1958","journal-title":"J. Biol. 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