{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T05:00:02Z","timestamp":1785301202890,"version":"3.55.0"},"reference-count":59,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2022,5,25]],"date-time":"2022-05-25T00:00:00Z","timestamp":1653436800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Fondation Sant\u00e9","award":["HFRI-FM17-3395"],"award-info":[{"award-number":["HFRI-FM17-3395"]}]},{"DOI":"10.13039\/501100013209","name":"Hellenic Foundation for Research and Innovation","doi-asserted-by":"publisher","award":["HFRI-FM17-3395"],"award-info":[{"award-number":["HFRI-FM17-3395"]}],"id":[{"id":"10.13039\/501100013209","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Bodossaki Foundation","award":["HFRI-FM17-3395"],"award-info":[{"award-number":["HFRI-FM17-3395"]}]},{"name":"Research Committee of the University of Patras","award":["HFRI-FM17-3395"],"award-info":[{"award-number":["HFRI-FM17-3395"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells"],"abstract":"<jats:p>Two main stem cell pools exist in the postnatal mammalian brain that, although they share some \u201cstemness\u201d properties, also exhibit significant differences. Multipotent neural stem cells survive within specialized microenvironments, called niches, and they are vulnerable to ageing. Oligodendroglial lineage-restricted progenitor cells are widely distributed in the brain parenchyma and are more resistant to the effects of ageing. Here, we create polymorphic neural stem cell cultures and allow cells to progress towards the neuronal and the oligodendroglial lineage. We show that the divergence of cell fate is accompanied by a divergence in the properties of progenitors, which reflects their adaptation to life in the niche or the parenchyma. Neurogenesis shows significant spatial restrictions and a dependence on laminin, a major niche component, while oligodendrogenesis shows none of these constraints. Furthermore, the blocking of integrin-\u03b21 leads to opposing effects, reducing neurogenesis and enhancing oligodendrogenesis. Therefore, polymorphic neural stem cell assays can be used to investigate the divergence of postnatal brain stem cells and also to predict the in vivo effects of potential therapeutic molecules targeting stem and progenitor cells, as we do for the microneurotrophin BNN-20.<\/jats:p>","DOI":"10.3390\/cells11111743","type":"journal-article","created":{"date-parts":[[2022,5,25]],"date-time":"2022-05-25T08:41:33Z","timestamp":1653468093000},"page":"1743","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Divergence between Neuronal and Oligodendroglial Cell Fate, in Postnatal Brain Neural Stem Cells, Leads to Divergent Properties in Polymorphic In Vitro Assays"],"prefix":"10.3390","volume":"11","author":[{"given":"Maria","family":"Anesti","sequence":"first","affiliation":[{"name":"Laboratory of Developmental Biology, Department of Biology, University of Patras, 26504 Patras, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stavroula","family":"Magkafa","sequence":"additional","affiliation":[{"name":"Laboratory of Developmental Biology, Department of Biology, University of Patras, 26504 Patras, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Efstathia","family":"Prantikou","sequence":"additional","affiliation":[{"name":"Laboratory of Developmental Biology, Department of Biology, University of Patras, 26504 Patras, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1035-0584","authenticated-orcid":false,"given":"Ilias","family":"Kazanis","sequence":"additional","affiliation":[{"name":"Laboratory of Developmental Biology, Department of Biology, University of Patras, 26504 Patras, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,5,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1007\/7854_2012_227","article-title":"Neurogenesis in the Adult Mammalian Brain: How Much Do We Need, How Much Do We Have?","volume":"Volume 15","author":"Belzung","year":"2012","journal-title":"Neurogenesis and Neural Plasticity"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1007\/s00441-017-2658-0","article-title":"Home Sweet Home: The Neural Stem Cell Niche throughout Development and after Injury","volume":"371","author":"Ruddy","year":"2018","journal-title":"Cell Tissue Res"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1016\/j.tins.2005.03.005","article-title":"Integrating New Neurons into the Adult Olfactory Bulb: Joining the Network, Life\u2013Death Decisions, and the Effects of Sensory Experience","volume":"28","author":"Lledo","year":"2005","journal-title":"Trends Neurosci."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"12302","DOI":"10.1523\/JNEUROSCI.3383-09.2009","article-title":"Turnover of Newborn Olfactory Bulb Neurons Optimizes Olfaction","volume":"29","author":"Mouret","year":"2009","journal-title":"J. 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