{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,13]],"date-time":"2026-07-13T13:20:52Z","timestamp":1783948852526,"version":"3.55.0"},"reference-count":25,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2022,5,30]],"date-time":"2022-05-30T00:00:00Z","timestamp":1653868800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Ministry of Science, Innovation and Universities, Government of Spain","award":["FPU19\/03393"],"award-info":[{"award-number":["FPU19\/03393"]}]},{"name":"Ministry of Science, Innovation and Universities, Government of Spain","award":["FPU20\/03621"],"award-info":[{"award-number":["FPU20\/03621"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells"],"abstract":"<jats:p>Clinical outcome in assisted reproduction techniques (ARTs) is mainly influenced by the quality of gametes used. It is known that a high percentage of sperm DNA fragmentation (DNAf) decreases the success of ART clinical results. Therefore, techniques such as magnetic-activated cell sorting (MACS) help to improve results in cases of patients with a high percentage of DNAf. Cryopreservation of sperm in donor intrauterine insemination (D-IUI) treatments increases sperm DNAf, so patients using these sperm samples can benefit from using this technique. This prospective randomized national multicenter study analyzed clinical outcomes of 181 D-IUI treatments. MACS was performed after density gradient centrifugation (DGC) in 90 thawed semen donor samples (MACSG), whereas only DGC was performed in 91 thawed semen donor samples (CG). To our knowledge, this is the first study analyzing the effect of MACS on D-IUI cycles. Our results show no significant differences in gestation, live birth, or miscarriage rates between the two groups. We believe that further studies with a larger sample size are needed to evaluate the application of MACS in combination with standard IUI donor sperm preparations in fertility clinics.<\/jats:p>","DOI":"10.3390\/cells11111794","type":"journal-article","created":{"date-parts":[[2022,5,30]],"date-time":"2022-05-30T10:05:14Z","timestamp":1653905114000},"page":"1794","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Effect of Sperm Selection by Magnetic-Activated Cell Sorting in D-IUI: A Randomized Control Trial"],"prefix":"10.3390","volume":"11","author":[{"given":"Cristina","family":"Gonz\u00e1lez-Ravina","sequence":"first","affiliation":[{"name":"IVI-RMA Seville, Avda. Am\u00e9rico Vespucio 19, 41092 Seville, Spain"},{"name":"IVI Foundation, Instituto de Investigaci\u00f3n Sanitaria La Fe (IIS La Fe), 46026 Valencia, Spain"},{"name":"Departamento de Biolog\u00eda Molecular e Ingenier\u00eda Bioqu\u00edmica, Universidad Pablo de Olavide, 41013 Seville, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7727-9116","authenticated-orcid":false,"given":"Esther","family":"Santamar\u00eda-L\u00f3pez","sequence":"additional","affiliation":[{"name":"IVI-RMA Seville, Avda. Am\u00e9rico Vespucio 19, 41092 Seville, Spain"},{"name":"IVI Foundation, Instituto de Investigaci\u00f3n Sanitaria La Fe (IIS La Fe), 46026 Valencia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alberto","family":"Pacheco","sequence":"additional","affiliation":[{"name":"IVI-RMA Madrid, Avenida del Talgo 68, 28023 Madrid, Spain"},{"name":"Facultad Ciencias de la Salud, Universidad Alfonso X \u201cEl Sabio\u201d, Villanueva de la Ca\u00f1ada, 28016 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julia","family":"Ramos","sequence":"additional","affiliation":[{"name":"IVI-RMA Seville, Avda. Am\u00e9rico Vespucio 19, 41092 Seville, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francisco","family":"Carranza","sequence":"additional","affiliation":[{"name":"IVI-RMA Seville, Avda. Am\u00e9rico Vespucio 19, 41092 Seville, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9801-9345","authenticated-orcid":false,"given":"Luc\u00eda","family":"Murria","sequence":"additional","affiliation":[{"name":"IVI Foundation, Instituto de Investigaci\u00f3n Sanitaria La Fe (IIS La Fe), 46026 Valencia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3275-1610","authenticated-orcid":false,"given":"Ana","family":"Ortiz-Vallecillo","sequence":"additional","affiliation":[{"name":"IVI Foundation, Instituto de Investigaci\u00f3n Sanitaria La Fe (IIS La Fe), 46026 Valencia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1214-2984","authenticated-orcid":false,"given":"Manuel","family":"Fern\u00e1ndez-S\u00e1nchez","sequence":"additional","affiliation":[{"name":"IVI-RMA Seville, Avda. Am\u00e9rico Vespucio 19, 41092 Seville, Spain"},{"name":"IVI Foundation, Instituto de Investigaci\u00f3n Sanitaria La Fe (IIS La Fe), 46026 Valencia, Spain"},{"name":"Departamento de Biolog\u00eda Molecular e Ingenier\u00eda Bioqu\u00edmica, Universidad Pablo de Olavide, 41013 Seville, Spain"},{"name":"Departamento de Cirug\u00eda, Universidad de Sevilla, Avda. S\u00e1nchez Pizjuan S\/N, 41009 Seville, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,5,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"382","DOI":"10.1111\/jog.14539","article-title":"Does the use of microfluidic sperm sorting for the sperm selection improve in vitro fertilization success rates in male factor infertility?","volume":"47","author":"Ozcan","year":"2021","journal-title":"J. Obstet. Gynaecol. 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