{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,16]],"date-time":"2026-05-16T15:05:31Z","timestamp":1778943931290,"version":"3.51.4"},"reference-count":23,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2022,6,1]],"date-time":"2022-06-01T00:00:00Z","timestamp":1654041600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000038","name":"National Sciences and Engineering Research Council of Canada","doi-asserted-by":"publisher","award":["RGPIN\/06101-2014"],"award-info":[{"award-number":["RGPIN\/06101-2014"]}],"id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000038","name":"National Sciences and Engineering Research Council of Canada","doi-asserted-by":"publisher","award":["OG24054"],"award-info":[{"award-number":["OG24054"]}],"id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000038","name":"National Sciences and Engineering Research Council of Canada","doi-asserted-by":"publisher","award":["706201"],"award-info":[{"award-number":["706201"]}],"id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009326","name":"Cancer Research Society","doi-asserted-by":"publisher","award":["RGPIN\/06101-2014"],"award-info":[{"award-number":["RGPIN\/06101-2014"]}],"id":[{"id":"10.13039\/100009326","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009326","name":"Cancer Research Society","doi-asserted-by":"publisher","award":["OG24054"],"award-info":[{"award-number":["OG24054"]}],"id":[{"id":"10.13039\/100009326","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009326","name":"Cancer Research Society","doi-asserted-by":"publisher","award":["706201"],"award-info":[{"award-number":["706201"]}],"id":[{"id":"10.13039\/100009326","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Canadian Cancer Research Institute","award":["RGPIN\/06101-2014"],"award-info":[{"award-number":["RGPIN\/06101-2014"]}]},{"name":"Canadian Cancer Research Institute","award":["OG24054"],"award-info":[{"award-number":["OG24054"]}]},{"name":"Canadian Cancer Research Institute","award":["706201"],"award-info":[{"award-number":["706201"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells"],"abstract":"<jats:p>Mesenchymal stromal cells (MSCs) are commonly known for their immune-suppressive abilities. However, our group provided evidence that it is possible to convert MSCs into potent antigen presenting cells (APCs) using either genetic engineering or pharmacological means. Given the capacity of UM171a to trigger APC-like function in MSCs, and the recent finding that this drug may modulate the epigenome by inhibiting the lysine-specific demethylase 1 (LSD1), we explored whether the direct pharmacological inhibition of LSD1 could instill APC-like functions in MSCs akin to UM171a. The treatment of MSCs with the LSD1 inhibitor tranylcypromine (TC) elicits a double-stranded (ds)RNA stress response along with its associated responsive elements, including pattern recognition receptors (PRRs), Type-I interferon (IFN), and IFN-stimulated genes (ISGs). The net outcome culminates in the enhanced expression of H2-Kb, and an increased stability of the cell surface peptide: MHCI complexes. As a result, TC-treated MSCs stimulate CD8 T-cell activation efficiently, and elicit potent anti-tumoral responses against the EG.7 T-cell lymphoma in the context of prophylactic vaccination. Altogether, our findings reveal a new pharmacological protocol whereby targeting LSD1 in MSCs elicits APC-like capabilities that could be easily exploited in the design of future MSC-based anti-cancer vaccines.<\/jats:p>","DOI":"10.3390\/cells11111816","type":"journal-article","created":{"date-parts":[[2022,6,1]],"date-time":"2022-06-01T12:48:31Z","timestamp":1654087711000},"page":"1816","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["LSD1 Inhibition Enhances the Immunogenicity of Mesenchymal Stromal Cells by Eliciting a dsRNA Stress Response"],"prefix":"10.3390","volume":"11","author":[{"given":"Fatemeh","family":"Mardani","sequence":"first","affiliation":[{"name":"Department of Pharmacology and Physiology, Universit\u00e9 de Montr\u00e9al, Montr\u00e9al, QC H3T 1J4, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wael","family":"Saad","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Physiology, Universit\u00e9 de Montr\u00e9al, Montr\u00e9al, QC H3T 1J4, Canada"},{"name":"Department of Chemistry and Biochemistry, Lebanese University, Hadat, Lebanon"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nehme","family":"El-Hachem","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Physiology, Universit\u00e9 de Montr\u00e9al, Montr\u00e9al, QC H3T 1J4, Canada"},{"name":"Pediatric Hematology-Oncology Division, Centre Hospitalier Universitaire Sainte-Justine Research, Montr\u00e9al, QC H3T 1C5, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jean-Pierre","family":"Bikorimana","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Infectious Diseases and Immunology, Universit\u00e9 de Montr\u00e9al, Montr\u00e9al, QC H3T 1J4, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mazen","family":"Kurdi","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry, Lebanese University, Hadat, Lebanon"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Riam","family":"Shammaa","sequence":"additional","affiliation":[{"name":"Intellistem Technologies Inc., Toronto, ON M5R 3N5, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9932-7174","authenticated-orcid":false,"given":"Sebastien","family":"Talbot","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Physiology, Universit\u00e9 de Montr\u00e9al, Montr\u00e9al, QC H3T 1J4, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4457-1350","authenticated-orcid":false,"given":"Moutih","family":"Rafei","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Physiology, Universit\u00e9 de Montr\u00e9al, Montr\u00e9al, QC H3T 1J4, Canada"},{"name":"Department of Microbiology, Infectious Diseases and Immunology, Universit\u00e9 de Montr\u00e9al, Montr\u00e9al, QC H3T 1J4, Canada"},{"name":"Molecular Biology Program, Universit\u00e9 de Montr\u00e9al, Montr\u00e9al, QC H3T 1J4, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,6,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"eaam7828","DOI":"10.1126\/scitranslmed.aam7828","article-title":"Apoptosis in mesenchymal stromal cells induces in vivo recipient-mediated immunomodulation","volume":"9","author":"Galleu","year":"2017","journal-title":"Sci. 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