{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,30]],"date-time":"2026-10-30T15:12:31Z","timestamp":1793373151314,"version":"4.5.0"},"reference-count":73,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2022,6,2]],"date-time":"2022-06-02T00:00:00Z","timestamp":1654128000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Deutsche Forschungsgemeinschaft (DFG), Bonn, Germany","award":["280809505"],"award-info":[{"award-number":["280809505"]}]},{"name":"Deutsche Forschungsgemeinschaft (DFG), Bonn, Germany","award":["436883643 (CH 109\/22 and CH 109\/26)"],"award-info":[{"award-number":["436883643 (CH 109\/22 and CH 109\/26)"]}]},{"name":"Deutsche Forschungsgemeinschaft (DFG), Bonn, Germany","award":["DFG RO 3714\/4-1"],"award-info":[{"award-number":["DFG RO 3714\/4-1"]}]},{"name":"Deutsche Forschungsgemeinschaft (DFG), Bonn, Germany","award":["RO957\/10-1"],"award-info":[{"award-number":["RO957\/10-1"]}]},{"name":"Deutsche Forschungsgemeinschaft (DFG), Bonn, Germany","award":["LOEWE\/DRUID Flex Funds 2021"],"award-info":[{"award-number":["LOEWE\/DRUID Flex Funds 2021"]}]},{"name":"Deutsche Forschungsgemeinschaft (DFG), Bonn, Germany","award":["2019-2022 #66-0008"],"award-info":[{"award-number":["2019-2022 #66-0008"]}]},{"name":"von-Behring-R\u00f6ntgen Foundation","award":["280809505"],"award-info":[{"award-number":["280809505"]}]},{"name":"von-Behring-R\u00f6ntgen Foundation","award":["436883643 (CH 109\/22 and CH 109\/26)"],"award-info":[{"award-number":["436883643 (CH 109\/22 and CH 109\/26)"]}]},{"name":"von-Behring-R\u00f6ntgen Foundation","award":["DFG RO 3714\/4-1"],"award-info":[{"award-number":["DFG RO 3714\/4-1"]}]},{"name":"von-Behring-R\u00f6ntgen Foundation","award":["RO957\/10-1"],"award-info":[{"award-number":["RO957\/10-1"]}]},{"name":"von-Behring-R\u00f6ntgen Foundation","award":["LOEWE\/DRUID Flex Funds 2021"],"award-info":[{"award-number":["LOEWE\/DRUID Flex Funds 2021"]}]},{"name":"von-Behring-R\u00f6ntgen Foundation","award":["2019-2022 #66-0008"],"award-info":[{"award-number":["2019-2022 #66-0008"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells"],"abstract":"<jats:p>Mesenchymal stromal cells (MSC) increasingly emerge as an option to ameliorate non-alcoholic steatohepatitis (NASH), a serious disease, which untreated may progress to liver cirrhosis and cancer. Before clinical translation, the mode of action of MSC needs to be established. Here, we established NASH in an immune-deficient mouse model by feeding a high fat diet. Human bone-marrow-derived MSC were delivered to the liver via intrasplenic transplantation. As verified by biochemical and image analyses, human mesenchymal stromal cells improved high-fat-diet-induced NASH in the mouse liver by decreasing hepatic lipid content and inflammation, as well as by restoring tissue homeostasis. MSC-mediated changes in gene expression indicated the switch from lipid storage to lipid utilization. It was obvious that host mouse hepatocytes harbored human mitochondria. Thus, it is feasible that resolution of NASH in mouse livers involved the donation of human mitochondria to the mouse hepatocytes. Therefore, human MSC might provide oxidative capacity for lipid breakdown followed by restoration of metabolic and tissue homeostasis.<\/jats:p>","DOI":"10.3390\/cells11111829","type":"journal-article","created":{"date-parts":[[2022,6,2]],"date-time":"2022-06-02T11:05:02Z","timestamp":1654167902000},"page":"1829","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["Human Mesenchymal Stromal Cells Resolve Lipid Load in High Fat Diet-Induced Non-Alcoholic Steatohepatitis in Mice by Mitochondria Donation"],"prefix":"10.3390","volume":"11","author":[{"given":"Sandra","family":"Nickel","sequence":"first","affiliation":[{"name":"Department of Visceral, Transplant, Thoracic and Vascular Surgery, University of Leipzig Medical Center, 04103 Leipzig, Germany"},{"name":"Division of General, Visceral and Vascular Surgery, University Hospital Jena, 07747 Jena, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Madlen","family":"Christ","sequence":"additional","affiliation":[{"name":"Department of Visceral, Transplant, Thoracic and Vascular Surgery, University of Leipzig Medical Center, 04103 Leipzig, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandra","family":"Schmidt","sequence":"additional","affiliation":[{"name":"Department of Visceral, Transplant, Thoracic and Vascular Surgery, University of Leipzig Medical Center, 04103 Leipzig, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0177-836X","authenticated-orcid":false,"given":"Joanna","family":"Kosacka","sequence":"additional","affiliation":[{"name":"Department of Visceral, Transplant, Thoracic and Vascular Surgery, University of Leipzig Medical Center, 04103 Leipzig, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hagen","family":"K\u00fchne","sequence":"additional","affiliation":[{"name":"Department of Visceral, Transplant, Thoracic and Vascular Surgery, University of Leipzig Medical Center, 04103 Leipzig, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3232-1277","authenticated-orcid":false,"given":"Martin","family":"Roderfeld","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, Justus-Liebig-University, 35392 Giessen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8888-1030","authenticated-orcid":false,"given":"Thomas","family":"Longerich","sequence":"additional","affiliation":[{"name":"Institute of Pathology, Heidelberg University Hospital, 69120 Heidelberg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2934-1880","authenticated-orcid":false,"given":"Lysann","family":"Tietze","sequence":"additional","affiliation":[{"name":"Department of Visceral, Transplant, Thoracic and Vascular Surgery, University of Leipzig Medical Center, 04103 Leipzig, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ina","family":"Bosse","sequence":"additional","affiliation":[{"name":"Department of Visceral, Transplant, Thoracic and Vascular Surgery, University of Leipzig Medical Center, 04103 Leipzig, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mei-Ju","family":"Hsu","sequence":"additional","affiliation":[{"name":"Department of Visceral, Transplant, Thoracic and Vascular Surgery, University of Leipzig Medical Center, 04103 Leipzig, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9208-8411","authenticated-orcid":false,"given":"Peggy","family":"Stock","sequence":"additional","affiliation":[{"name":"Department of Visceral, Transplant, Thoracic and Vascular Surgery, University of Leipzig Medical Center, 04103 Leipzig, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5739-5400","authenticated-orcid":false,"given":"Elke","family":"Roeb","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, Justus-Liebig-University, 35392 Giessen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8213-9548","authenticated-orcid":false,"given":"Bruno","family":"Christ","sequence":"additional","affiliation":[{"name":"Department of Visceral, Transplant, Thoracic and Vascular Surgery, University of Leipzig Medical Center, 04103 Leipzig, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,6,2]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1002\/hep.28431","article-title":"Global epidemiology of nonalcoholic fatty liver disease-meta-analytic assessment of prevalence, incidence, and outcomes","volume":"64","author":"Younossi","year":"2016","journal-title":"Hepatology"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"637","DOI":"10.1016\/j.bpg.2014.07.008","article-title":"Non-alcoholic fatty liver disease, obesity and the metabolic syndrome","volume":"28","author":"Dietrich","year":"2014","journal-title":"Best Pract. 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