{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T16:57:17Z","timestamp":1793120237658,"version":"4.5.0"},"reference-count":35,"publisher":"MDPI AG","issue":"15","license":[{"start":{"date-parts":[[2022,7,22]],"date-time":"2022-07-22T00:00:00Z","timestamp":1658448000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Deutsche Forschungsgemeinschaft (DFG)","award":["HA 6655\/1-3"],"award-info":[{"award-number":["HA 6655\/1-3"]}]},{"name":"Deutsche Forschungsgemeinschaft (DFG)","award":["AU 635\/1-1"],"award-info":[{"award-number":["AU 635\/1-1"]}]},{"name":"Deutsche Forschungsgemeinschaft (DFG)","award":["BU 2918\/3-1"],"award-info":[{"award-number":["BU 2918\/3-1"]}]},{"name":"Deutsche Forschungsgemeinschaft (DFG)","award":["387509280"],"award-info":[{"award-number":["387509280"]}]},{"name":"Deutsche Forschungsgemeinschaft (DFG)","award":["SFB 1350"],"award-info":[{"award-number":["SFB 1350"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells"],"abstract":"<jats:p>(1) Background: Autosomal dominant polycystic kidney disease (ADPKD) is a frequent monogenic disorder that leads to progressive renal cyst growth and renal failure. Strategies to inhibit cyst growth in non-human cyst models have often failed in clinical trials. There is a significant need for models that enable studies of human cyst growth and drug trials. (2) Methods: Renal tissue from ADPKD patients who received a nephrectomy as well as adult mouse kidney slices were cultured on a chorioallantoic membrane (CAM) for one week. The cyst volume was monitored by microscopic and CT-based applications. The weight and angiogenesis were quantified. Morphometric and histological analyses were performed after the removal of the tissues from the CAM. (3) Results: The mouse and human renal tissue mostly remained vital for about one week on the CAM. The growth of cystic tissue was evaluated using microscopic and CT-based volume measurements, which correlated with weight and an increase in angiogenesis, and was accompanied by cyst cell proliferation. (4) Conclusions: The CAM model might bridge the gap between animal studies and clinical trials of human cyst growth, and provide a drug-testing platform for the inhibition of cyst enlargement. Real-time analyses of mouse kidney tissue may provide insights into renal physiology and reduce the need for animal experiments.<\/jats:p>","DOI":"10.3390\/cells11152269","type":"journal-article","created":{"date-parts":[[2022,7,22]],"date-time":"2022-07-22T12:53:45Z","timestamp":1658494425000},"page":"2269","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["A 3D In Vivo Model for Studying Human Renal Cystic Tissue and Mouse Kidney Slices"],"prefix":"10.3390","volume":"11","author":[{"given":"Eva-Marie","family":"Bichlmayer","sequence":"first","affiliation":[{"name":"Institute for Molecular and Cellular Anatomy, University of Regensburg, 93053 Regensburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lina","family":"Mahl","sequence":"additional","affiliation":[{"name":"Institute for Molecular and Cellular Anatomy, University of Regensburg, 93053 Regensburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leo","family":"Hesse","sequence":"additional","affiliation":[{"name":"Institute for Molecular and Cellular Anatomy, University of Regensburg, 93053 Regensburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric","family":"Pion","sequence":"additional","affiliation":[{"name":"Institute for Molecular and Cellular Anatomy, University of Regensburg, 93053 Regensburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Victoria","family":"Haller","sequence":"additional","affiliation":[{"name":"Institute for Molecular and Cellular Anatomy, University of Regensburg, 93053 Regensburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andreas","family":"Moehwald","sequence":"additional","affiliation":[{"name":"Institute for Molecular and Cellular Anatomy, University of Regensburg, 93053 Regensburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christina","family":"Hackl","sequence":"additional","affiliation":[{"name":"Department of Surgery, University Hospital Regensburg, Franz-Josef-Strauss-Allee 11, 93053 Regensburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jens M.","family":"Werner","sequence":"additional","affiliation":[{"name":"Department of Surgery, University Hospital Regensburg, Franz-Josef-Strauss-Allee 11, 93053 Regensburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3874-0296","authenticated-orcid":false,"given":"Hans J.","family":"Schlitt","sequence":"additional","affiliation":[{"name":"Department of Surgery, University Hospital Regensburg, Franz-Josef-Strauss-Allee 11, 93053 Regensburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3404-4397","authenticated-orcid":false,"given":"Siegfried","family":"Schwarz","sequence":"additional","affiliation":[{"name":"KML Vision GmbH, A-8020 Graz, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7970-370X","authenticated-orcid":false,"given":"Philipp","family":"Kainz","sequence":"additional","affiliation":[{"name":"KML Vision GmbH, A-8020 Graz, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6761-0247","authenticated-orcid":false,"given":"Christoph","family":"Brochhausen","sequence":"additional","affiliation":[{"name":"Institute of Pathology, University of Regensburg, 93053 Regensburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"Groeger","sequence":"additional","affiliation":[{"name":"Department for Radiotherapy, University Hospital Regensburg, Franz-Josef-Strauss-Allee 11, 93053 Regensburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Felix","family":"Steger","sequence":"additional","affiliation":[{"name":"Department for Radiotherapy, University Hospital Regensburg, Franz-Josef-Strauss-Allee 11, 93053 Regensburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oliver","family":"K\u00f6lbl","sequence":"additional","affiliation":[{"name":"Department for Radiotherapy, University Hospital Regensburg, Franz-Josef-Strauss-Allee 11, 93053 Regensburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6803-4755","authenticated-orcid":false,"given":"Christoph","family":"Daniel","sequence":"additional","affiliation":[{"name":"Department of Nephropathology, Friedrich-Alexander University Erlangen-N\u00fcrnberg, 91054 Erlangen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kerstin","family":"Amann","sequence":"additional","affiliation":[{"name":"Department of Nephropathology, Friedrich-Alexander University Erlangen-N\u00fcrnberg, 91054 Erlangen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andre","family":"Kraus","sequence":"additional","affiliation":[{"name":"Department of Nephrology and Hypertension, University Hospital Erlangen, University of Erlangen-Nuremberg, 91054 Erlangen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bj\u00f6rn","family":"Buchholz","sequence":"additional","affiliation":[{"name":"Department of Nephrology and Hypertension, University Hospital Erlangen, University of Erlangen-Nuremberg, 91054 Erlangen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thiha","family":"Aung","sequence":"additional","affiliation":[{"name":"Institute for Molecular and Cellular Anatomy, University of Regensburg, 93053 Regensburg, Germany"},{"name":"Faculty of Applied Healthcare Science, Deggendorf Institute of Technology, 94469 Deggendorf, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9440-3307","authenticated-orcid":false,"given":"Silke","family":"Haerteis","sequence":"additional","affiliation":[{"name":"Institute for Molecular and Cellular Anatomy, University of Regensburg, 93053 Regensburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,7,22]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1146\/annurev.med.60.101707.125712","article-title":"Polycystic kidney disease","volume":"60","author":"Harris","year":"2009","journal-title":"Annu. 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