{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T13:38:29Z","timestamp":1787578709485,"version":"build-2736575974"},"reference-count":30,"publisher":"MDPI AG","issue":"15","license":[{"start":{"date-parts":[[2022,8,2]],"date-time":"2022-08-02T00:00:00Z","timestamp":1659398400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82171033"],"award-info":[{"award-number":["82171033"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81970781"],"award-info":[{"award-number":["81970781"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81800869"],"award-info":[{"award-number":["81800869"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["LD21H120001"],"award-info":[{"award-number":["LD21H120001"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004731","name":"Natural Science Foundation of Zhejiang Province","doi-asserted-by":"publisher","award":["82171033"],"award-info":[{"award-number":["82171033"]}],"id":[{"id":"10.13039\/501100004731","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004731","name":"Natural Science Foundation of Zhejiang Province","doi-asserted-by":"publisher","award":["81970781"],"award-info":[{"award-number":["81970781"]}],"id":[{"id":"10.13039\/501100004731","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004731","name":"Natural Science Foundation of Zhejiang Province","doi-asserted-by":"publisher","award":["81800869"],"award-info":[{"award-number":["81800869"]}],"id":[{"id":"10.13039\/501100004731","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004731","name":"Natural Science Foundation of Zhejiang Province","doi-asserted-by":"publisher","award":["LD21H120001"],"award-info":[{"award-number":["LD21H120001"]}],"id":[{"id":"10.13039\/501100004731","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells"],"abstract":"<jats:p>Background: Posterior capsule opacification (PCO) is the most common complication after cataract surgery, in which increased levels of transforming growth factor-beta 2 (TGF-\u03b22) accelerate PCO formation; however, the pathological mechanisms are not fully understood. This study aims to explore the regulation mechanism of TGF-\u03b22 in PCO formation via its autophagic functions. Methods: The autophagic effect of TGF-\u03b22 was detected by transmission electron microscopy (TEM), Western blotting, and immunofluorescence analysis. The association between autophagy and the epithelial\u2013mesenchymal transition (EMT) was evaluated by qPCR and Western blotting. The transcriptome analysis was used to uncover the molecular mechanism of TGF-\u03b22-induced PCO formation. Results: TGF-\u03b22 specifically promotes autophagy flux in human lens epithelial cells. The activation of autophagy by rapamycin can promote EMT marker synthesis and improve cell migration. However, the inhibition of autophagy by 3-MA attenuates EMT. To uncover the molecular mechanisms, we performed RNA sequencing and found that TGF-\u03b22 elevated tumor protein p53-inducible nuclear protein2 (TP53INP2) expression, which was accompanied by a nuclear-to-cytoplasm translocation. Moreover, the knockdown of TP53INP2 blocked the TGF-\u03b22-induced autophagy and EMT processes, revealing that TP53INP2 plays an important role in TGF-\u03b22-induced autophagy during EMT. Conclusions: Taken together, the results of this study suggested that TP53INP2 was a novel regulator of PCO development by TGF-\u03b22, and notably, TP53INP2, may be a potential target for the pharmacological treatment of PCO.<\/jats:p>","DOI":"10.3390\/cells11152385","type":"journal-article","created":{"date-parts":[[2022,8,2]],"date-time":"2022-08-02T21:41:37Z","timestamp":1659476497000},"page":"2385","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["TP53INP2 Contributes to TGF-\u03b22-Induced Autophagy during the Epithelial\u2013Mesenchymal Transition in Posterior Capsular Opacification Development"],"prefix":"10.3390","volume":"11","author":[{"given":"Yilei","family":"Cui","sequence":"first","affiliation":[{"name":"Eye Center, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310009, China"},{"name":"Zhejiang Provincial Key Lab of Ophthalmology, Hangzhou 310009, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hao","family":"Yang","sequence":"additional","affiliation":[{"name":"Eye Center, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310009, China"},{"name":"Zhejiang Provincial Key Lab of Ophthalmology, Hangzhou 310009, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silu","family":"Shi","sequence":"additional","affiliation":[{"name":"Eye Center, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310009, China"},{"name":"Zhejiang Provincial Key Lab of Ophthalmology, Hangzhou 310009, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiyuan","family":"Ping","sequence":"additional","affiliation":[{"name":"Eye Center, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310009, China"},{"name":"Zhejiang Provincial Key Lab of Ophthalmology, Hangzhou 310009, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sifan","family":"Zheng","sequence":"additional","affiliation":[{"name":"GKT School of Medical Education, King\u2019s College London, London WC2R 2LS, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiajing","family":"Tang","sequence":"additional","affiliation":[{"name":"Eye Center, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310009, China"},{"name":"Zhejiang Provincial Key Lab of Ophthalmology, Hangzhou 310009, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoning","family":"Yu","sequence":"additional","affiliation":[{"name":"Eye Center, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310009, China"},{"name":"Zhejiang Provincial Key Lab of Ophthalmology, Hangzhou 310009, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xingchao","family":"Shentu","sequence":"additional","affiliation":[{"name":"Eye Center, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310009, China"},{"name":"Zhejiang Provincial Key Lab of Ophthalmology, Hangzhou 310009, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,8,2]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Konopinska, J., Mlynarczyk, M., Dmuchowska, D.A., and Obuchowska, I. 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