{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,11]],"date-time":"2026-07-11T05:53:38Z","timestamp":1783749218633,"version":"3.55.0"},"reference-count":147,"publisher":"MDPI AG","issue":"15","license":[{"start":{"date-parts":[[2022,8,4]],"date-time":"2022-08-04T00:00:00Z","timestamp":1659571200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells"],"abstract":"<jats:p>The specific NKCC1 cotransporter antagonist, bumetanide, attenuates the severity of Autism Spectrum Disorders (ASD), and many neurodevelopmental or neurodegenerative disorders in animal models and clinical trials. However, the pervasive expression of NKCC1 in many cell types throughout the body is thought to challenge the therapeutic efficacy of bumetanide. However, many peripheral functions, including intestinal, metabolic, or vascular, etc., are perturbed in brain disorders contributing to the neurological sequels. Alterations of these functions also increase the incidence of the disorder suggesting complex bidirectional links with the clinical manifestations. We suggest that a more holistic view of ASD and other disorders is warranted to account for the multiple sites impacted by the original intra-uterine insult. From this perspective, large-spectrum active repositioned drugs that act centrally and peripherally might constitute a useful approach to treating these disorders.<\/jats:p>","DOI":"10.3390\/cells11152419","type":"journal-article","created":{"date-parts":[[2022,8,4]],"date-time":"2022-08-04T21:52:48Z","timestamp":1659649968000},"page":"2419","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["A Wholistic View of How Bumetanide Attenuates Autism Spectrum Disorders"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3702-1599","authenticated-orcid":false,"given":"Eric","family":"Delpire","sequence":"first","affiliation":[{"name":"Departments of Anesthesiology and Molecular Physiology & Biophysics, Vanderbilt University School of Medicine, Nashville, TN 37232, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6208-8480","authenticated-orcid":false,"given":"Yehezkel","family":"Ben-Ari","sequence":"additional","affiliation":[{"name":"NeuroChlore, Campus Scientifique de Luminy, 163 Route de Luminy, 13273 Marseilles, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,8,4]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1640","DOI":"10.1136\/bmj.2.5425.1640","article-title":"Today\u2019s drugs. Frusemide","volume":"2","author":"Ingram","year":"1964","journal-title":"Br. Med. J."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1136\/bmj.1.5794.211","article-title":"Bumetanide: Potent new \u201cloop\u201d diuretic","volume":"1","author":"Asbury","year":"1972","journal-title":"Br. Med. J."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"17713","DOI":"10.1016\/S0021-9258(17)32499-7","article-title":"Molecular cloning, primary structure, and characterization of two members of the mammalian electroneutral sodium-(potassium)-chloride cotransporter family expressed in kidney","volume":"269","author":"Gamba","year":"1994","journal-title":"J. Biol. Chem."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"17","DOI":"10.5049\/EBP.2015.13.1.17","article-title":"Loop Diuretics in Clinical Practice","volume":"13","author":"Oh","year":"2015","journal-title":"Electrolyte Blood Press."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1159\/000480977","article-title":"Protein binding characteristics of bumetanide","volume":"12","author":"Walker","year":"1989","journal-title":"Dev. Pharmacol. Ther."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1002\/bdd.267","article-title":"Pharmacokinetics and pharmacodynamics of intravenous bumetanide in mutant Nagase analbuminemic rats: Importance of globulin binding for the pharmacodynamic effects","volume":"22","author":"Kim","year":"2001","journal-title":"Biopharm. Drug Dispos."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"192","DOI":"10.1038\/9713","article-title":"Deafness and imbalance associated with inactivation of the secretory Na-K-2Cl co-transporter","volume":"22","author":"Delpire","year":"1999","journal-title":"Nat. Genet."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"26946","DOI":"10.1074\/jbc.274.38.26946","article-title":"Mice lacking the basolateral Na-K-2Cl cotransporter have impaired epithelial chloride secretion and are profoundly deaf","volume":"274","author":"Flagella","year":"1999","journal-title":"J. Biol. Chem."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"C615","DOI":"10.1152\/ajpcell.2001.281.2.C615","article-title":"Alterations in airway ion transport in NKCC1-deficient mice","volume":"281","author":"Grubb","year":"2001","journal-title":"Am. J. Physiol. Cell Physiol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"G550","DOI":"10.1152\/ajpgi.00095.2005","article-title":"Role of Na-K-2Cl cotransporter-1 in gastric secretion of nonacidic fluid and pepsinogen","volume":"289","author":"McDaniel","year":"2005","journal-title":"Am. J. Physiol. Gastrointest. Liver Physiol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"G707","DOI":"10.1152\/ajpgi.2000.279.4.G707","article-title":"Intestinal ion transport in NKCC1-deficient mice","volume":"279","author":"Grubb","year":"2000","journal-title":"Am. J. Physiol. Gastrointest. Liver Physiol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"723","DOI":"10.1172\/JCI118844","article-title":"Expression of the mouse Na-K-2Cl cotransporter, mBSC2, in the terminal IMCD, the glomerular and extraglomerular mesangium and the glomerular afferent arteriole","volume":"98","author":"Kaplan","year":"1996","journal-title":"J. Clin. Investig."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"F619","DOI":"10.1152\/ajprenal.1996.271.3.F619","article-title":"Localization and regulation of the rat renal Na+-K+-2Cl\u2212 cotransporter, BSC-1","volume":"271","author":"Ecelbarger","year":"1996","journal-title":"Am. J. Physiol. Ren. Physiol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"26720","DOI":"10.1016\/S0021-9258(19)61435-3","article-title":"Severe impairment of salivation in Na+\/K+\/2Cl\u2212 cotransporter (NKCC1)-deficient mice","volume":"275","author":"Evans","year":"2000","journal-title":"J. Biol. Chem."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"F1155","DOI":"10.1152\/ajprenal.00709.2012","article-title":"Na+-K+-2Cl\u2212 cotransporter-mediated fluid secretion increases under hypotonic osmolarity in the mouse submandibular salivary gland","volume":"306","author":"Kidokoro","year":"2014","journal-title":"Am. J. Physiol. Ren. Physiol."},{"key":"ref_16","first-page":"196","article-title":"Riding the tides: K+ concentration and volume regulation by muscle Na+-K+-2Cl\u2212 cotransport activity","volume":"18","author":"Gosmanov","year":"2003","journal-title":"News Physiol. Sci."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"H2100","DOI":"10.1152\/ajpheart.01402.2006","article-title":"Effect of the Na-K-2Cl cotransporter NKCC1 on systemic blood pressure and smooth muscle tone","volume":"292","author":"Garg","year":"2007","journal-title":"Am. J. Physiol. Heart Circ. Physiol."},{"key":"ref_18","first-page":"5861","article-title":"Cl\u2212 channels in smooth muscle cells","volume":"466","author":"Bulley","year":"2014","journal-title":"Pfl\u00fcgers Arch. Eur. J. Physiol."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"T\u00f3th, K., L\u00e9n\u00e1rt, N., Berki, P., Fekete, R., Szabadits, E., P\u00f3sfai, B., Cser\u00e9p, C., Alatshan, A., Benk\u0151, S., and Kiss, D. (2022). The NKCC1 ion transporter modulates microglial phenotype and inflammatory response to brain injury in a cell-autonomous manner. PLoS Biol., 20.","DOI":"10.1371\/journal.pbio.3001526"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1016\/j.bcp.2018.08.013","article-title":"Bumetanide attenuates acute lung injury by suppressing macrophage activation","volume":"156","author":"Hung","year":"2018","journal-title":"Biochem. Pharmacol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1532","DOI":"10.1038\/s41556-019-0431-1","article-title":"Interpreting an apoptotic corpse as anti-inflammatory involves a chloride sensing pathway","volume":"21","author":"Perry","year":"2019","journal-title":"Nat. Cell Biol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"441","DOI":"10.1172\/JCI8553","article-title":"Failure of spermatogenesis in mouse lines deficient in the Na+-K+-2Cl\u2212 cotransporter","volume":"105","author":"Pace","year":"2000","journal-title":"J. Clin. Investig."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1215","DOI":"10.1152\/physrev.00017.2006","article-title":"GABA: A pioneer transmitter that excites immature neurons and generates primitive oscillations","volume":"87","author":"Gaiarsa","year":"2007","journal-title":"Physiol. Rev."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"7531","DOI":"10.1523\/JNEUROSCI.20-20-07531.2000","article-title":"Abnormal GABAA-receptor mediated currents in dorsal root ganglion neurons isolated from Na-K-2Cl cotransporter null mice","volume":"20","author":"Sung","year":"2000","journal-title":"J. Neurosci."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"384","DOI":"10.1038\/ng1002","article-title":"Mutations in the K-Cl cotransporter KCC3 cause a severe peripheral neuropathy associated with agenesis of the corpus callosum","volume":"32","author":"Howard","year":"2002","journal-title":"Nat. Genet."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1016\/j.nbd.2007.06.014","article-title":"Axonal and periaxonal swelling precede peripheral neurodegeneration in KCC3 knockout mice","volume":"28","author":"Byun","year":"2007","journal-title":"Neurobiol. Dis."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"16355","DOI":"10.1074\/jbc.274.23.16355","article-title":"Cloning and Characterization of KCC3 and KCC4, new members of the cation-chloride cotransporter gene family","volume":"274","author":"Mount","year":"1999","journal-title":"J. Biol. Chem."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1016\/j.neuroscience.2012.09.040","article-title":"GABAergic control of retinal ganglion cell dendritic development","volume":"227","author":"Chabrol","year":"2012","journal-title":"Neuroscience"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1523\/JNEUROSCI.16-01-00082.1996","article-title":"Regulation of intracellulat Cl\u2212 levels by Na+-dependent Cl\u2212 cotransport distinguishes depolarizing from hyperpolarizing GABAA receptor-mediated responses in spinal neurons","volume":"16","author":"Rohrbough","year":"1996","journal-title":"J. Neurosci."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1113\/jphysiol.1989.sp017762","article-title":"Giant synaptic potentials in immature rat CA3 hippocampal neurones","volume":"416","author":"Cherubini","year":"1989","journal-title":"J. Physiol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"728","DOI":"10.1038\/nrn920","article-title":"Excitatory actions of gaba during development: The nature of the nurture","volume":"3","year":"2002","journal-title":"Nat. Rev. Neurosci."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"523","DOI":"10.1016\/S0166-2236(97)01147-8","article-title":"GABAA, NMDA and AMPA receptors: A developmentally regulated \u2018m\u00e9nage \u00e0 trois\u2019","volume":"20","author":"Khazipov","year":"1997","journal-title":"Trends Neurosci."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"554","DOI":"10.1126\/science.aac6472","article-title":"A GABAergic projection from the zona incerta to cortex promotes cortical neuron development","volume":"350","author":"Chen","year":"2015","journal-title":"Science"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1016\/S0165-3806(98)00078-9","article-title":"Ontogeny of cation-Cl\u2212 cotransporter expression in rat neocortex","volume":"109","author":"Clayton","year":"1998","journal-title":"Brain Res. Dev. Brain Res."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"558","DOI":"10.1002\/(SICI)1097-4695(19990615)39:4<558::AID-NEU9>3.0.CO;2-5","article-title":"Developmental regulation of the neuronal-specific isoform of K-Cl cotransporter KCC2 in postnatal rat brains","volume":"39","author":"Lu","year":"1999","journal-title":"J. Neurobiol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"781","DOI":"10.1002\/(SICI)1097-4695(19971120)33:6<781::AID-NEU6>3.0.CO;2-5","article-title":"Expression of the Na-K-2Cl cotransporter is developmentally regulated in postnatal rat brains: A possible mechanism underlying GABA\u2019s excitatory role in immature brain","volume":"33","author":"Plotkin","year":"1997","journal-title":"J. Neurobiol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"536","DOI":"10.1016\/j.tins.2017.07.001","article-title":"NKCC1 Chloride Importer Antagonists Attenuate Many Neurological and Psychiatric Disorders","volume":"40","year":"2017","journal-title":"Trends Neurosci."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"C541","DOI":"10.1152\/ajpcell.00102.2011","article-title":"Ion channels and transporters [corrected] in cancer. 2. Ion channels and the control of cancer cell migration","volume":"301","author":"Cuddapah","year":"2011","journal-title":"Am. J. Physiol. Cell Physiol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"1009","DOI":"10.1016\/j.tips.2021.09.005","article-title":"Pharmacological tools to target NKCC1 in brain disorders","volume":"42","author":"Savardi","year":"2021","journal-title":"Trends Pharmacol. Sci."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"11088","DOI":"10.1523\/JNEUROSCI.1234-11.2011","article-title":"Expression of GABA signaling molecules KCC2, NKCC1, and GAD1 in cortical development and schizophrenia","volume":"31","author":"Hyde","year":"2011","journal-title":"J. Neurosci."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"932","DOI":"10.1038\/s43587-021-00122-7","article-title":"Experimental and real-world evidence supporting the computational repurposing of bumetanide for APOE4-related Alzheimer\u2019s disease","volume":"1","author":"Taubes","year":"2021","journal-title":"Nat. Aging"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1152\/jn.00865.2015","article-title":"Kinase-KCC2 coupling: Cl\u2212 rheostasis, disease susceptibility, therapeutic target","volume":"115","author":"Kahle","year":"2016","journal-title":"J. Neurophysiol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"751","DOI":"10.1073\/pnas.1524013113","article-title":"KCC2 rescues functional deficits in human neurons derived from patients with Rett syndrome","volume":"113","author":"Tang","year":"2016","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Pisella, L.I., Gaiarsa, J.L., Diabira, D., Zhang, J., Khalilov, I., Duan, J., Kahle, K.T., and Medina, I. (2019). Impaired regulation of KCC2 phosphorylation leads to neuronal network dysfunction and neurodevelopmental pathology. Sci. Signal., 12.","DOI":"10.1126\/scisignal.aay0300"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"675","DOI":"10.1126\/science.1247190","article-title":"Oxytocin-Mediated GABA Inhibition During Delivery Attenuates Autism Pathogenesis in Rodent Offspring","volume":"343","author":"Tyzio","year":"2014","journal-title":"Science"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"620","DOI":"10.1126\/science.1250214","article-title":"Neuroscience. Could autism be treated prenatally?","volume":"343","author":"Zimmerman","year":"2014","journal-title":"Science"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"318","DOI":"10.1038\/nm.3827","article-title":"Reversing excitatory GABAAR signaling restores synaptic plasticity and memory in a mouse model of Down syndrome","volume":"21","author":"Deidda","year":"2015","journal-title":"Nat. Med."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"9276","DOI":"10.1038\/s41598-019-45635-9","article-title":"Early alterations in a mouse model of Rett syndrome: The GABA developmental shift is abolished at birth","volume":"9","author":"Lozovaya","year":"2019","journal-title":"Sci. Rep."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"3982","DOI":"10.1093\/cercor\/bhy279","article-title":"The GABA Developmental Shift Is Abolished by Maternal Immune Activation Already at Birth","volume":"29","author":"Fernandez","year":"2019","journal-title":"Cereb. Cortex"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"564","DOI":"10.1038\/s41582-021-00530-8","article-title":"Maternal immune activation and neuroinflammation in human neurodevelopmental disorders","volume":"17","author":"Han","year":"2021","journal-title":"Nat. Rev. Neurol."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"11878","DOI":"10.1038\/s41598-017-12183-z","article-title":"Bumetanide treatment during early development rescues maternal separation-induced susceptibility to stress","volume":"7","author":"Hu","year":"2017","journal-title":"Sci. Rep."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"e202","DOI":"10.1038\/tp.2012.124","article-title":"A randomised controlled trial of bumetanide in the treatment of autism in children","volume":"2","author":"Lemonnier","year":"2012","journal-title":"Transl. Psychiatry"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"e1056","DOI":"10.1038\/tp.2017.10","article-title":"Effects of bumetanide on neurobehavioral function in children and adolescents with autism spectrum disorders","volume":"7","author":"Lemonnier","year":"2017","journal-title":"Transl. Psychiatry"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"751575","DOI":"10.3389\/fpsyt.2021.751575","article-title":"Treatment Effect of Bumetanide in Children With Autism Spectrum Disorder: A Systematic Review and Meta-Analysis","volume":"12","author":"Wang","year":"2021","journal-title":"Front. Psychiatry"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"3163","DOI":"10.1038\/s41598-017-03378-5","article-title":"Look me in the eyes: Constraining gaze in the eye-region provokes abnormally high subcortical activation in autism","volume":"7","author":"Hadjikhani","year":"2017","journal-title":"Sci. Rep."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"3602","DOI":"10.1038\/s41598-018-21958-x","article-title":"Bumetanide for autism: More eye contact, less amygdala activation","volume":"8","author":"Hadjikhani","year":"2018","journal-title":"Sci. Rep."},{"key":"ref_57","unstructured":"Juarez-Martinez, E.L., Sprengers, J.J., Cristian, G., Oranje, B., van Andel, D.M., Avramiea, A.E., Simpraga, S., Houtman, S.J., Hardstone, R., and Gerver, C. (2021). Prediction of Behavioral Improvement Through Resting-State Electroencephalography and Clinical Severity in a Randomized Controlled Trial Testing Bumetanide in Autism Spectrum Disorder. Biol. Psychiatry Cogn. Neurosci. Neuroimaging."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"228","DOI":"10.1038\/s41398-022-01987-x","article-title":"The immuno-behavioural covariation associated with the treatment response to bumetanide in young children with autism spectrum disorder","volume":"12","author":"Li","year":"2022","journal-title":"Transl. Psychiatry"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1097\/WNF.0000000000000136","article-title":"Treating Schizophrenia With the Diuretic Bumetanide: A Case Report","volume":"39","author":"Lemonnier","year":"2016","journal-title":"Clin. Neuropharmacol."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"e288","DOI":"10.1111\/apa.12235","article-title":"Treating Fragile X syndrome with the diuretic bumetanide: A case report","volume":"102","author":"Lemonnier","year":"2013","journal-title":"Acta Paediatr."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1186\/s13229-020-00335-4","article-title":"Effects of bumetanide on neurodevelopmental impairments in patients with tuberous sclerosis complex: An open-label pilot study","volume":"11","author":"Sprengers","year":"2020","journal-title":"Mol. Autism"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"e539","DOI":"10.1542\/peds.2014-4133","article-title":"Paradoxical Benzodiazepine Response: A Rationale for Bumetanide in Neurodevelopmental Disorders?","volume":"136","author":"Bruining","year":"2015","journal-title":"Pediatrics"},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"987","DOI":"10.1093\/brain\/awr041","article-title":"Neuronal chloride accumulation and excitatory GABA underlie aggravation of neonatal epileptiform activities by phenobarbital","volume":"134","author":"Nardou","year":"2011","journal-title":"Brain"},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"1422","DOI":"10.1038\/s41467-018-03802-y","article-title":"GABAergic inhibition in dual-transmission cholinergic and GABAergic striatal interneurons is abolished in Parkinson disease","volume":"9","author":"Lozovaya","year":"2018","journal-title":"Nat. Commun."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1097\/WNF.0000000000000114","article-title":"Bumetanide to Treat Parkinson Disease: A Report of 4 Cases","volume":"39","author":"Damier","year":"2016","journal-title":"Clin. Neuropharmacol."},{"key":"ref_66","doi-asserted-by":"crossref","unstructured":"Cleary, R.T., Sun, H., Huynh, T., Manning, S.M., Li, Y., Rotenberg, A., Talos, D.M., Kahle, K.T., Jackson, M., and Rakhade, S.N. (2013). Bumetanide enhances phenobarbital efficacy in a rat model of hypoxic neonatal seizures. PLoS ONE, 8.","DOI":"10.1371\/annotation\/48a011e6-e4d0-4706-9a28-857eba8cfb31"},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"182","DOI":"10.1016\/j.neuropharm.2017.02.006","article-title":"Multiple blood-brain barrier transport mechanisms limit bumetanide accumulation, and therapeutic potential, in the mammalian brain","volume":"117","author":"Fedrowitz","year":"2017","journal-title":"Neuropharmacology"},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"941","DOI":"10.1111\/epi.16866","article-title":"Reply to the commentary by Ben-Ari and Delpire: Bumetanide and neonatal seizures: Fiction versus reality","volume":"62","author":"Kaila","year":"2021","journal-title":"Epilepsia"},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"1868","DOI":"10.1111\/epi.17279","article-title":"Bumetanide for neonatal seizures: No light in the pharmacokinetic\/dynamic tunnel","volume":"63","author":"Kaila","year":"2022","journal-title":"Epilepsia"},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"46","DOI":"10.3389\/fphar.2012.00046","article-title":"Barrier mechanisms in the developing brain","volume":"3","author":"Saunders","year":"2012","journal-title":"Front. Pharmacol."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1002\/cne.20885","article-title":"Functional effectiveness of the blood-brain barrier to small water-soluble molecules in developing and adult opossum (Monodelphis domestica)","volume":"496","author":"Ek","year":"2006","journal-title":"J. Comp. Neurol."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1002\/cne.901380104","article-title":"Development of the blood vessels and extracellular spaces during postnatal maturation of rat cerebral cortex","volume":"138","author":"Caley","year":"1970","journal-title":"J. Comp. Neurol."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"833","DOI":"10.2174\/1381612033455314","article-title":"Effect of diabetes on the blood brain barrier","volume":"9","author":"Horani","year":"2003","journal-title":"Curr. Pharm. Des."},{"key":"ref_74","first-page":"125","article-title":"Diabetes Mellitus and Blood-Brain Barrier Dysfunction: An Overview","volume":"2","author":"Prasad","year":"2014","journal-title":"J. Pharmacovigil."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"612104","DOI":"10.3389\/fimmu.2020.612104","article-title":"The Joint-Brain Axis: Insights From Rheumatoid Arthritis on the Crosstalk Between Chronic Peripheral Inflammation and the Brain","volume":"11","author":"Rothe","year":"2020","journal-title":"Front. Immunol."},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1186\/s12974-021-02086-2","article-title":"Changes of blood-brain-barrier function and transfer of amyloid beta in rats with collagen-induced arthritis","volume":"18","author":"Lai","year":"2021","journal-title":"J. Neuroinflamm."},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"2712","DOI":"10.1016\/j.devcel.2021.08.010","article-title":"Tumor-induced disruption of the blood-brain barrier promotes host death","volume":"56","author":"Kim","year":"2021","journal-title":"Dev. Cell"},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1016\/j.brainres.2015.05.026","article-title":"Physical training improves non-spatial memory, locomotor skills and the blood brain barrier in diabetic rats","volume":"1618","author":"Xavier","year":"2015","journal-title":"Brain Res."},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"733","DOI":"10.1139\/apnm-2017-0775","article-title":"Exercise and taurine in inflammation, cognition, and peripheral markers of blood-brain barrier integrity in older women","volume":"43","author":"Chupel","year":"2018","journal-title":"Appl. Physiol. Nutr. Metab."},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1038\/nrneurol.2017.188","article-title":"Blood-brain barrier breakdown in Alzheimer disease and other neurodegenerative disorders","volume":"14","author":"Sweeney","year":"2018","journal-title":"Nat. Rev. Neurol."},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"1318","DOI":"10.1038\/s41593-018-0234-x","article-title":"The role of brain vasculature in neurodegenerative disorders","volume":"21","author":"Sweeney","year":"2018","journal-title":"Nat. Neurosci."},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1016\/j.jneuroim.2008.12.002","article-title":"Elevated immune response in the brain of autistic patients","volume":"207","author":"Li","year":"2009","journal-title":"J. Neuroimmunol."},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"320","DOI":"10.1016\/j.neuroscience.2013.08.025","article-title":"Brain IL-6 and autism","volume":"252","author":"Wei","year":"2013","journal-title":"Neuroscience"},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"e844","DOI":"10.1038\/tp.2016.77","article-title":"Atopic diseases and inflammation of the brain in the pathogenesis of autism spectrum disorders","volume":"6","author":"Theoharides","year":"2016","journal-title":"Transl. Psychiatry"},{"key":"ref_85","doi-asserted-by":"crossref","first-page":"68","DOI":"10.3109\/1547691X.2010.545086","article-title":"Theoretical aspects of autism: Causes\u2014A review","volume":"8","author":"Ratajczak","year":"2011","journal-title":"J. Immunotoxicol."},{"key":"ref_86","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1186\/s13229-016-0110-z","article-title":"Blood-brain barrier and intestinal epithelial barrier alterations in autism spectrum disorders","volume":"7","author":"Fiorentino","year":"2016","journal-title":"Mol. Autism"},{"key":"ref_87","doi-asserted-by":"crossref","first-page":"469","DOI":"10.1016\/S1474-4422(14)70303-5","article-title":"Bumetanide for the treatment of seizures in newborn babies with hypoxic ischaemic encephalopathy (NEMO): An open-label, dose finding, and feasibility phase 1\/2 trial","volume":"14","author":"Pressler","year":"2015","journal-title":"Lancet Neurol."},{"key":"ref_88","doi-asserted-by":"crossref","first-page":"995","DOI":"10.1016\/j.jpeds.2013.06.012","article-title":"Factors associated with permanent hearing impairment in infants treated with therapeutic hypothermia","volume":"163","author":"Smit","year":"2013","journal-title":"J. Pediatr."},{"key":"ref_89","doi-asserted-by":"crossref","first-page":"715","DOI":"10.1001\/archinte.1966.03870110107021","article-title":"Acute transient hearing loss after ethacrynic acid therapy","volume":"117","author":"Schneider","year":"1966","journal-title":"Arch. Int. Med."},{"key":"ref_90","first-page":"36","article-title":"Case report. Diuretic induced ototoxicity in preeclampsia","volume":"64","author":"Morrison","year":"1971","journal-title":"J. Tenn. Med. Assoc."},{"key":"ref_91","doi-asserted-by":"crossref","first-page":"587","DOI":"10.1002\/j.1552-4604.1981.tb05668.x","article-title":"Long-term bumetanide treatment of patients with edema due to renal disease. Cooperative studies","volume":"21","author":"Stone","year":"1981","journal-title":"J. Clin. Pharmacol."},{"key":"ref_92","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1016\/0378-5955(95)00009-S","article-title":"Ion transport mechanisms responsible for K+ secretion and the transepithelial voltage across marginal cells of stria vascularis in vitro","volume":"84","author":"Wangemann","year":"1995","journal-title":"Hear. Res."},{"key":"ref_93","doi-asserted-by":"crossref","first-page":"532","DOI":"10.1002\/humu.23722","article-title":"Kilquist Syndrome: A Novel Syndromic Hearing Loss Disorder Caused by Homozygous Deletion of SLC12A2","volume":"40","author":"Macnamara","year":"2019","journal-title":"Hum. Mut."},{"key":"ref_94","doi-asserted-by":"crossref","first-page":"e478","DOI":"10.1212\/NXG.0000000000000478","article-title":"SLC12A2 mutations cause NKCC1 deficiency with encephalopathy and impaired secretory epithelia","volume":"6","author":"Stodberg","year":"2020","journal-title":"Neurol. Genet."},{"key":"ref_95","doi-asserted-by":"crossref","first-page":"2380","DOI":"10.1093\/brain\/awaa176","article-title":"SLC12A2 variants cause a neurodevelopmental disorder or cochleovestibular defect","volume":"143","author":"McNeill","year":"2020","journal-title":"Brain"},{"key":"ref_96","doi-asserted-by":"crossref","unstructured":"Mutai, H., Wasano, K., Momozawa, Y., Kamatani, Y., Miya, F., Masuda, S., Morimoto, N., Nara, K., Takahashi, S., and Tsunoda, T. (2020). Variants encoding a restricted carboxy-terminal domain of SLC12A2 cause hereditary hearing loss in humans. PLoS Genet., 16.","DOI":"10.1371\/journal.pgen.1008643"},{"key":"ref_97","doi-asserted-by":"crossref","first-page":"1047","DOI":"10.3109\/00016489.2014.900704","article-title":"Age-related change in the expression of NKCC1 in the cochlear lateral wall of C57BL\/6J mice","volume":"134","author":"Liu","year":"2014","journal-title":"Acta Otolaryngol."},{"key":"ref_98","doi-asserted-by":"crossref","first-page":"547","DOI":"10.1016\/0028-3908(75)90119-7","article-title":"Cochlear N1 depression produced by the new \u201cloop\u201d diuretic, bumetanide, in cats","volume":"14","author":"Brown","year":"1975","journal-title":"Neuropharmacology"},{"key":"ref_99","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1016\/S0041-008X(79)80018-6","article-title":"Comparative acute ototoxicity of intravenous bumetanide and furosemide in the pure-bred beagle","volume":"48","author":"Brown","year":"1979","journal-title":"Toxicol. Appl. Pharmacol."},{"key":"ref_100","doi-asserted-by":"crossref","first-page":"917","DOI":"10.1016\/S0140-6736(76)92147-4","article-title":"Letter: Frusemide, bumetanide, and ototoxicity","volume":"1","author":"Bourke","year":"1976","journal-title":"Lancet"},{"key":"ref_101","doi-asserted-by":"crossref","first-page":"1300","DOI":"10.1002\/aur.1771","article-title":"Autism spectrum disorders and the amplitude of auditory brainstem response wave I","volume":"10","author":"Santos","year":"2017","journal-title":"Autism Res."},{"key":"ref_102","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1002\/(SICI)1096-9861(19960624)370:2<247::AID-CNE8>3.0.CO;2-2","article-title":"Embryological origin for autism: Developmental anomalies of the cranial nerve motor nuclei","volume":"370","author":"Rodier","year":"1996","journal-title":"J. Comp. Neurol."},{"key":"ref_103","doi-asserted-by":"crossref","first-page":"107974","DOI":"10.1016\/j.heares.2020.107974","article-title":"Three dimensional reconstructions of the superior olivary complex from children with autism spectrum disorder","volume":"393","author":"Mansour","year":"2020","journal-title":"Hear. Res."},{"key":"ref_104","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1016\/j.neuroscience.2013.12.050","article-title":"Depolarizing chloride gradient in developing cochlear nucleus neurons: Underlying mechanism and implication for calcium signaling","volume":"261","author":"Witte","year":"2014","journal-title":"Neuroscience"},{"key":"ref_105","doi-asserted-by":"crossref","first-page":"4646","DOI":"10.1523\/JNEUROSCI.18-12-04646.1998","article-title":"A developmental shift from GABAergic to glycinergic transmission in the central auditory system","volume":"18","author":"Kotak","year":"1998","journal-title":"J. Neurosci."},{"key":"ref_106","doi-asserted-by":"crossref","first-page":"651209","DOI":"10.3389\/fnins.2021.651209","article-title":"Auditory Discrimination in Autism Spectrum Disorder","volume":"15","author":"Rotschafer","year":"2021","journal-title":"Front. Neurosci."},{"key":"ref_107","first-page":"3","article-title":"Transport processes in the formation of the cerebrospinal fluid","volume":"83","author":"Wright","year":"1978","journal-title":"Rev. Physiol. Biochem. Pharmacol."},{"key":"ref_108","unstructured":"Johanson, C.E. (1995). Ventricles and Cerebrospinal Fluid. Neuroscience in Medicine, J. B. Lippincott & Co."},{"key":"ref_109","first-page":"324","article-title":"The effect of furosemide on cerebrospinal fluid flow in rabbits","volume":"178","author":"Reed","year":"1969","journal-title":"Arch. Int. Pharmacodyn. Ther."},{"key":"ref_110","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1016\/0014-4886(69)90005-3","article-title":"Effects of diuretics on cerebrospinal fluid formation and potassium movement","volume":"24","author":"Domer","year":"1969","journal-title":"Exp. Neurol."},{"key":"ref_111","doi-asserted-by":"crossref","first-page":"503","DOI":"10.1007\/BF00236592","article-title":"The effect of furosemide on sodium-22 uptake into cerebrospinal fluid and brain","volume":"14","author":"Buhrley","year":"1972","journal-title":"Exp. Brain Res."},{"key":"ref_112","doi-asserted-by":"crossref","first-page":"C1616","DOI":"10.1152\/ajpcell.1994.267.6.C1616","article-title":"Potassium cotransport at the rat choroid plexus","volume":"267","author":"Keep","year":"1994","journal-title":"Am. J. Physiol. Cell Physiol."},{"key":"ref_113","doi-asserted-by":"crossref","first-page":"C173","DOI":"10.1152\/ajpcell.1997.272.1.C173","article-title":"Expression of the Na+-K+-2Cl\u2212 cotransporter BSC2 in the nervous system","volume":"272","author":"Plotkin","year":"1997","journal-title":"Am. J. Physiol. Cell Physiol."},{"key":"ref_114","doi-asserted-by":"crossref","first-page":"C522","DOI":"10.1152\/ajpcell.00490.2018","article-title":"Elusive role of the Na-K-2Cl cotransporter in the choroid plexus","volume":"316","author":"Delpire","year":"2019","journal-title":"Am. J. Physiol. Cell Physiol."},{"key":"ref_115","doi-asserted-by":"crossref","first-page":"4733","DOI":"10.1113\/JP279867","article-title":"CrossTalk proposal: Apical NKCC1 of choroid plexus epithelial cells works in the net inward flux mode under basal conditions, maintaining intracellular Cl\u2212 and cell volume","volume":"598","year":"2020","journal-title":"J. Physiol."},{"key":"ref_116","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1227\/00006123-197901000-00009","article-title":"Effect of furosemide on experimental traumatic cerebral edema","volume":"4","author":"Tornheim","year":"1979","journal-title":"Neurosurgery"},{"key":"ref_117","doi-asserted-by":"crossref","first-page":"405","DOI":"10.1227\/00006123-198304000-00006","article-title":"Furosemide and mannitol in the treatment of acute experimental intracranial hypertension","volume":"12","author":"Wilkinson","year":"1983","journal-title":"Neurosurgery"},{"key":"ref_118","first-page":"5","article-title":"The lowering of cerebral spinal fluid and brain interstitial pressure of preterm and term rabbits by furosemide","volume":"40","author":"Greene","year":"1985","journal-title":"Z. Kinderchir."},{"key":"ref_119","first-page":"10","article-title":"Furosemide lowers intracranial pressure by inhibiting CSF production","volume":"41","author":"Lorenzo","year":"1986","journal-title":"Z. Kinderchir."},{"key":"ref_120","doi-asserted-by":"crossref","first-page":"208","DOI":"10.1227\/00006123-197908000-00003","article-title":"Cerebrospinal fluid dynamics in patients with pseudotumor cerebri","volume":"5","author":"Sklar","year":"1979","journal-title":"Neurosurgery"},{"key":"ref_121","doi-asserted-by":"crossref","unstructured":"Shulman, K., Marmarou, A., Miller, J.D., Becker, D.P., Hochwald, G.M., and Brock, M. (1980). The Effects of Furosemide on CSF Dynamics in Patients with Pseudotumor Cerebri. Intracranial Pressure IV, Springer.","DOI":"10.1007\/978-3-642-67543-0"},{"key":"ref_122","doi-asserted-by":"crossref","first-page":"C1383","DOI":"10.1152\/ajpcell.1999.276.6.C1383","article-title":"Vasoconstrictors and nitrovasodilators reciprocally regulate the Na+-K+-2Cl\u2212 cotransporter in rat aorta","volume":"276","author":"Akar","year":"1999","journal-title":"Am. J. Physiol."},{"key":"ref_123","doi-asserted-by":"crossref","first-page":"F409","DOI":"10.1152\/ajprenal.00309.2005","article-title":"Hypotension in NKCC1 null mice: Role of the kidneys","volume":"290","author":"Wall","year":"2006","journal-title":"Am. J. Physiol. Ren. Physiol."},{"key":"ref_124","doi-asserted-by":"crossref","first-page":"835","DOI":"10.1111\/j.1651-2227.2012.02699.x","article-title":"Unexpected extra-renal effects of loop diuretics in the preterm neonate","volume":"101","author":"Cotton","year":"2012","journal-title":"Acta Paediatr."},{"key":"ref_125","doi-asserted-by":"crossref","first-page":"622","DOI":"10.3171\/2009.11.JNS081052","article-title":"Molecular mechanisms of microvascular failure in central nervous system injury--synergistic roles of NKCC1 and SUR1\/TRPM4","volume":"113","author":"Simard","year":"2010","journal-title":"J. Neurosurg."},{"key":"ref_126","doi-asserted-by":"crossref","first-page":"511","DOI":"10.1083\/jcb.135.2.511","article-title":"Chloride ion efflux regulates adherence, spreading, and respiratory burst of neutrophils stimulated by tumor necrosis factor-alpha (TNF) on biologic surfaces","volume":"135","author":"Menegazzi","year":"1996","journal-title":"J. Cell Biol."},{"key":"ref_127","doi-asserted-by":"crossref","first-page":"997","DOI":"10.1038\/nm.4361","article-title":"Inflammation-dependent cerebrospinal fluid hypersecretion by the choroid plexus epithelium in posthemorrhagic hydrocephalus","volume":"23","author":"Karimy","year":"2017","journal-title":"Nat. Med."},{"key":"ref_128","doi-asserted-by":"crossref","first-page":"641993","DOI":"10.3389\/fnmol.2021.641993","article-title":"Inhibition of the NKCC1\/NF-\u03baB Signaling Pathway Decreases Inflammation and Improves Brain Edema and Nerve Cell Apoptosis in an SBI Rat Model","volume":"14","author":"Gong","year":"2021","journal-title":"Front. Mol. Neurosci."},{"key":"ref_129","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1016\/j.bbr.2008.12.016","article-title":"Immune involvement in schizophrenia and autism: Etiology, pathology and animal models","volume":"204","author":"Patterson","year":"2009","journal-title":"Behav. Brain Res."},{"key":"ref_130","doi-asserted-by":"crossref","first-page":"e1447","DOI":"10.1542\/peds.2012-1107","article-title":"Autism after infection, febrile episodes, and antibiotic use during pregnancy: An exploratory study","volume":"130","author":"Henriksen","year":"2012","journal-title":"Pediatrics"},{"key":"ref_131","doi-asserted-by":"crossref","first-page":"12776","DOI":"10.1073\/pnas.1202556109","article-title":"Modeling an autism risk factor in mice leads to permanent immune dysregulation","volume":"109","author":"Hsiao","year":"2012","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_132","doi-asserted-by":"crossref","first-page":"1451","DOI":"10.1016\/j.cell.2013.11.024","article-title":"Microbiota modulate behavioral and physiological abnormalities associated with neurodevelopmental disorders","volume":"155","author":"Hsiao","year":"2013","journal-title":"Cell"},{"key":"ref_133","doi-asserted-by":"crossref","first-page":"933","DOI":"10.1126\/science.aad0314","article-title":"The maternal interleukin-17a pathway in mice promotes autism-like phenotypes in offspring","volume":"351","author":"Choi","year":"2016","journal-title":"Science"},{"key":"ref_134","doi-asserted-by":"crossref","first-page":"607","DOI":"10.1016\/j.bbi.2012.01.011","article-title":"Maternal immune activation yields offspring displaying mouse versions of the three core symptoms of autism","volume":"26","author":"Malkova","year":"2012","journal-title":"Brain Behav. Immun."},{"key":"ref_135","doi-asserted-by":"crossref","first-page":"680","DOI":"10.1016\/j.biopsych.2017.09.030","article-title":"Maternal Immune Activation Delays Excitatory-to-Inhibitory Gamma-Aminobutyric Acid Switch in Offspring","volume":"83","author":"Corradini","year":"2018","journal-title":"Biol. Psychiatry"},{"key":"ref_136","doi-asserted-by":"crossref","first-page":"509","DOI":"10.1016\/j.molmed.2014.05.002","article-title":"Microbiota and neurodevelopmental windows: Implications for brain disorders","volume":"20","author":"Borre","year":"2014","journal-title":"Trends Mol. Med."},{"key":"ref_137","first-page":"263","article-title":"Na+-K+-2Cl\u2212 symporter contributes to \u03b3-aminobutyric acid-evoked excitation in rat enteric neurons","volume":"72","author":"Liu","year":"2020","journal-title":"Sheng Li Xue Bao"},{"key":"ref_138","doi-asserted-by":"crossref","first-page":"761","DOI":"10.1113\/jphysiol.2005.098962","article-title":"The neurotransmitters glycine and GABA stimulate glucagon-like peptide-1 release from the GLUTag cell line","volume":"569","author":"Gameiro","year":"2005","journal-title":"J. Physiol."},{"key":"ref_139","doi-asserted-by":"crossref","first-page":"e12684","DOI":"10.1111\/jne.12684","article-title":"The role of the gut microbiota in development, function and disorders of the central nervous system and the enteric nervous system","volume":"31","author":"Heiss","year":"2019","journal-title":"J. Neuroendocrinol."},{"key":"ref_140","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1038\/nn.4476","article-title":"Interactions between the microbiota, immune and nervous systems in health and disease","volume":"20","author":"Fung","year":"2017","journal-title":"Nat. Neurosci."},{"key":"ref_141","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1016\/B978-0-12-418700-9.00009-5","article-title":"Immune dysregulation in autism spectrum disorder","volume":"113","author":"Hsiao","year":"2013","journal-title":"Int. Rev. Neurobiol."},{"key":"ref_142","doi-asserted-by":"crossref","unstructured":"Iglesias-V\u00e1zquez, L., Van Ginkel Riba, G., Arija, V., and Canals, J. (2020). Composition of Gut Microbiota in Children with Autism Spectrum Disorder: A Systematic Review and Meta-Analysis. Nutrients, 12.","DOI":"10.3390\/nu12030792"},{"key":"ref_143","doi-asserted-by":"crossref","first-page":"5748","DOI":"10.1038\/ncomms6748","article-title":"Transcriptome analysis reveals dysregulation of innate immune response genes and neuronal activity-dependent genes in autism","volume":"5","author":"Gupta","year":"2014","journal-title":"Nat. Commun."},{"key":"ref_144","doi-asserted-by":"crossref","first-page":"915","DOI":"10.1016\/j.cell.2016.10.027","article-title":"The Central Nervous System and the Gut Microbiome","volume":"167","author":"Sharon","year":"2016","journal-title":"Cell"},{"key":"ref_145","doi-asserted-by":"crossref","first-page":"eaat5236","DOI":"10.1126\/science.aat5236","article-title":"A gut-brain neural circuit for nutrient sensory transduction","volume":"361","author":"Kaelberer","year":"2018","journal-title":"Science"},{"key":"ref_146","doi-asserted-by":"crossref","first-page":"3097","DOI":"10.1113\/jphysiol.2008.153148","article-title":"Molecular, pharmacological and functional properties of GABA(A) receptors in anterior pituitary cells","volume":"586","author":"Zemkova","year":"2008","journal-title":"J. Physiol."},{"key":"ref_147","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1186\/2040-2392-2-16","article-title":"Investigation of the serum levels of anterior pituitary hormones in male children with autism","volume":"2","author":"Iwata","year":"2011","journal-title":"Mol. Autism"}],"container-title":["Cells"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2073-4409\/11\/15\/2419\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T00:04:22Z","timestamp":1760141062000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2073-4409\/11\/15\/2419"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,8,4]]},"references-count":147,"journal-issue":{"issue":"15","published-online":{"date-parts":[[2022,8]]}},"alternative-id":["cells11152419"],"URL":"https:\/\/doi.org\/10.3390\/cells11152419","relation":{},"ISSN":["2073-4409"],"issn-type":[{"value":"2073-4409","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,8,4]]}}}