{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T06:17:24Z","timestamp":1790749044286,"version":"4.1.0"},"reference-count":50,"publisher":"MDPI AG","issue":"16","license":[{"start":{"date-parts":[[2022,8,9]],"date-time":"2022-08-09T00:00:00Z","timestamp":1660003200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002426","name":"Italian Telethon ONLUS","doi-asserted-by":"publisher","award":["GGP19231"],"award-info":[{"award-number":["GGP19231"]}],"id":[{"id":"10.13039\/501100002426","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002426","name":"Italian Telethon ONLUS","doi-asserted-by":"publisher","award":["AR059646"],"award-info":[{"award-number":["AR059646"]}],"id":[{"id":"10.13039\/501100002426","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002426","name":"Italian Telethon ONLUS","doi-asserted-by":"publisher","award":["#2015ZZR4W3"],"award-info":[{"award-number":["#2015ZZR4W3"]}],"id":[{"id":"10.13039\/501100002426","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health USA","doi-asserted-by":"publisher","award":["GGP19231"],"award-info":[{"award-number":["GGP19231"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health USA","doi-asserted-by":"publisher","award":["AR059646"],"award-info":[{"award-number":["AR059646"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health USA","doi-asserted-by":"publisher","award":["#2015ZZR4W3"],"award-info":[{"award-number":["#2015ZZR4W3"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Italian Ministry of University and Research","award":["GGP19231"],"award-info":[{"award-number":["GGP19231"]}]},{"name":"Italian Ministry of University and Research","award":["AR059646"],"award-info":[{"award-number":["AR059646"]}]},{"name":"Italian Ministry of University and Research","award":["#2015ZZR4W3"],"award-info":[{"award-number":["#2015ZZR4W3"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells"],"abstract":"<jats:p>Calsequestrin 1 (CASQ1) and Ryanodine receptor 1 (RYR1) are two of the main players in excitation\u2013contraction (EC) coupling. CASQ1-knockout mice and mice carrying a mutation in RYR1 (Y522S) linked to human malignant hyperthermia susceptibility (MHS) both suffer lethal hypermetabolic episodes when exposed to halothane (MHS crises) and to environmental heat (heat stroke, HS). The phenotype of Y522S is more severe than that of CASQ1-null mice. As MHS and HS are hypermetabolic responses, we studied the metabolism of adult CASQ1-null and Y522S mice using wild-type (WT) mice as controls. We found that CASQ1-null and Y522S mice have increased food consumption and higher core temperature at rest. By indirect calorimetry, we then verified that CASQ1-null and Y522S mice show an increased oxygen consumption and a lower respiratory quotient (RQ). The accelerated metabolism of CASQ1-null and Y522S mice was also accompanied with a reduction in body fat. Moreover, both mouse models displayed increased oxygen consumption and a higher core temperature during heat stress. The results collected suggest that metabolic rate, oxygen consumption, and body temperature at rest, all more elevated in Y522S than in CASQ1-null mice, could possibly be used as predictors of the level of susceptibility to hyperthermic crises of mice (and possibly humans).<\/jats:p>","DOI":"10.3390\/cells11162468","type":"journal-article","created":{"date-parts":[[2022,8,10]],"date-time":"2022-08-10T02:42:53Z","timestamp":1660099373000},"page":"2468","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Oxygen Consumption and Basal Metabolic Rate as Markers of Susceptibility to Malignant Hyperthermia and Heat Stroke"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4151-2980","authenticated-orcid":false,"given":"Matteo","family":"Serano","sequence":"first","affiliation":[{"name":"CAST, Center for Advanced Studies and Technology, University G. d\u2019Annunzio of Chieti-Pescara, 66100 Chieti, Italy"},{"name":"DMSI, Department of Medicine and Aging Sciences, University G. d\u2019Annunzio of Chieti-Pescara, 66100 Chieti, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1197-7813","authenticated-orcid":false,"given":"Laura","family":"Pietrangelo","sequence":"additional","affiliation":[{"name":"CAST, Center for Advanced Studies and Technology, University G. d\u2019Annunzio of Chieti-Pescara, 66100 Chieti, Italy"},{"name":"DMSI, Department of Medicine and Aging Sciences, University G. d\u2019Annunzio of Chieti-Pescara, 66100 Chieti, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5720-7577","authenticated-orcid":false,"given":"Cecilia","family":"Paolini","sequence":"additional","affiliation":[{"name":"CAST, Center for Advanced Studies and Technology, University G. d\u2019Annunzio of Chieti-Pescara, 66100 Chieti, Italy"},{"name":"DNICS, Department of Neuroscience and Clinical Sciences, University G. d\u2019Annunzio of Chieti-Pescara, 66100 Chieti, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Flavia A.","family":"Guarnier","sequence":"additional","affiliation":[{"name":"Department of General Pathology, Londrina State University, Londrina 86057-970, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0213-7591","authenticated-orcid":false,"given":"Feliciano","family":"Protasi","sequence":"additional","affiliation":[{"name":"CAST, Center for Advanced Studies and Technology, University G. d\u2019Annunzio of Chieti-Pescara, 66100 Chieti, Italy"},{"name":"DMSI, Department of Medicine and Aging Sciences, University G. d\u2019Annunzio of Chieti-Pescara, 66100 Chieti, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,8,9]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1131","DOI":"10.1016\/S0140-6736(98)03078-5","article-title":"Malignant hyperthermia","volume":"352","author":"Denborough","year":"1998","journal-title":"Lancet"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"789","DOI":"10.1126\/science.256.5058.789","article-title":"Malignant hyperthermia","volume":"256","author":"MacLennan","year":"1992","journal-title":"Science"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"241","DOI":"10.4065\/70.3.241","article-title":"Clinical effects of intravenously administered dantrolene","volume":"70","author":"Wedel","year":"1995","journal-title":"Mayo Clin. 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