{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T16:41:37Z","timestamp":1790959297733,"version":"4.1.0"},"reference-count":55,"publisher":"MDPI AG","issue":"16","license":[{"start":{"date-parts":[[2022,8,12]],"date-time":"2022-08-12T00:00:00Z","timestamp":1660262400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81872567"],"award-info":[{"award-number":["81872567"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells"],"abstract":"<jats:p>Glycoproteins are involved in the development of many diseases, while the type and content of N-glycoproteins in the cartilage of osteoarthritis (OA) and Kashin\u2013Beck disease (KBD) are still unclear. This research aims to identify N-glycoproteins in knee cartilage patients with OA and KBD compared with normal control (N) adults. The cartilage samples were collected from gender- and age-matched OA (n = 9), KBD (n = 9) patients, and N (n = 9) adults. Glycoproteomics and label-free liquid chromatography-tandem mass spectrometry (LC-MS\/MS) obtained N-glycoproteins of KBD and OA. A total of 594 N-glycoproteins and 1146 N-glycosylation peptides were identified. The identified data were further compared and analyzed with Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), and Protein\u2013Protein Interactions (PPI). Pairwise comparison of the glycoproteins detected in the three groups showed that integrin beta-1 (ITGB1), collagen alpha-1 (II) chain (COL2A1), collagen alpha-1 (VII) chain (COL7A1), carbohydrate sulfotransferase 3 (CHST-3), carbohydrate sulfotransferase 4 (CHST-4), thrombospondin 2 (THBS2), bone morphogenetic protein 8A (BMP8A), tenascin-C (TNC), lysosome-associated membrane protein (LAMP2), and beta-glucuronidase (GUSB) were significantly differentially expressed. GO results suggested N-glycoproteins mainly belonged to protein metabolic process, single-multicellular and multicellular organism process, cell adhesion, biological adhesion, and multicellular organism development. KEGG and PPI results revealed that key N-glycoproteins were closely related to pathways for OA and KBD, such as phagosome, ECM-receptor interaction, lysosome, focal adhesion, protein digestion, and absorption. These results reflected glycoprotein expression for OA and KBD in the process of ECM degradation, material transport, cell\u2013cell or cell\u2013ECM interaction, and information transduction. These key significantly differentially expressed N-glycoproteins and pathways lead to the degeneration and degradation of the cartilage of OA and KBD mainly by disrupting the synthesis and catabolism of basic components of ECM and chondrocytes and interfering with the transfer of material or information. The key N-glycoproteins or pathways in this research are potential targets for pathological mechanisms and therapies of OA and KBD.<\/jats:p>","DOI":"10.3390\/cells11162513","type":"journal-article","created":{"date-parts":[[2022,8,14]],"date-time":"2022-08-14T21:09:06Z","timestamp":1660511346000},"page":"2513","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Identification of N-Glycoproteins of Knee Cartilage from Adult Osteoarthritis and Kashin-Beck Disease Based on Quantitative Glycoproteomics, Compared with Normal Control Cartilage"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1460-0114","authenticated-orcid":false,"given":"Jing","family":"Han","sequence":"first","affiliation":[{"name":"Department of Occupational and Environmental Health, School of Public Health, Health Science Center, Xi\u2019an Jiaotong University, Xi\u2019an 710061, China"},{"name":"Key Laboratory of Environment and Genes Related to Diseases, School of Public Health, Health Science Center, Xi\u2019an Jiaotong University, Xi\u2019an 710061, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4298-3581","authenticated-orcid":false,"given":"Huan","family":"Deng","sequence":"additional","affiliation":[{"name":"Department of Occupational and Environmental Health, School of Public Health, Health Science Center, Xi\u2019an Jiaotong University, Xi\u2019an 710061, China"},{"name":"Key Laboratory of Environment and Genes Related to Diseases, School of Public Health, Health Science Center, Xi\u2019an Jiaotong University, Xi\u2019an 710061, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8376-0747","authenticated-orcid":false,"given":"Yizhen","family":"Lyu","sequence":"additional","affiliation":[{"name":"Department of Occupational and Environmental Health, School of Public Health, Health Science Center, Xi\u2019an Jiaotong University, Xi\u2019an 710061, China"},{"name":"Key Laboratory of Environment and Genes Related to Diseases, School of Public Health, Health Science Center, Xi\u2019an Jiaotong University, Xi\u2019an 710061, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiang","family":"Xiao","sequence":"additional","affiliation":[{"name":"Department of Occupational and Environmental Health, School of Public Health, Health Science Center, Xi\u2019an Jiaotong University, Xi\u2019an 710061, China"},{"name":"Key Laboratory of Environment and Genes Related to Diseases, School of Public Health, Health Science Center, Xi\u2019an Jiaotong University, Xi\u2019an 710061, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Occupational and Environmental Health, School of Public Health, Health Science Center, Xi\u2019an Jiaotong University, Xi\u2019an 710061, China"},{"name":"Key Laboratory of Environment and Genes Related to Diseases, School of Public Health, Health Science Center, Xi\u2019an Jiaotong University, Xi\u2019an 710061, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiaxin","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Occupational and Environmental Health, School of Public Health, Health Science Center, Xi\u2019an Jiaotong University, Xi\u2019an 710061, China"},{"name":"Key Laboratory of Environment and Genes Related to Diseases, School of Public Health, Health Science Center, Xi\u2019an Jiaotong University, Xi\u2019an 710061, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ziwei","family":"Guo","sequence":"additional","affiliation":[{"name":"Department of Occupational and Environmental Health, School of Public Health, Health Science Center, Xi\u2019an Jiaotong University, Xi\u2019an 710061, China"},{"name":"Key Laboratory of Environment and 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Xi\u2019an Jiaotong University, Xi\u2019an 710061, China"},{"name":"Key Laboratory of Environment and Genes Related to Diseases, School of Public Health, Health Science Center, Xi\u2019an Jiaotong University, Xi\u2019an 710061, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hang","family":"Gao","sequence":"additional","affiliation":[{"name":"Laboratory of Resource Biology and Biotechnology in Western China (Ministry of Education), Provincial Key Laboratory of Biotechnology, College of Life Sciences, Northwest University, Xi\u2019an 710069, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6181-9904","authenticated-orcid":false,"given":"Mikko Juhani","family":"Lammi","sequence":"additional","affiliation":[{"name":"Department of Occupational and Environmental Health, School of Public Health, Health Science Center, Xi\u2019an Jiaotong University, Xi\u2019an 710061, China"},{"name":"Department of Integrative 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