{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,13]],"date-time":"2026-01-13T21:55:28Z","timestamp":1768341328200,"version":"3.49.0"},"reference-count":65,"publisher":"MDPI AG","issue":"17","license":[{"start":{"date-parts":[[2022,8,30]],"date-time":"2022-08-30T00:00:00Z","timestamp":1661817600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005297","name":"Medical University of Bialystok","doi-asserted-by":"publisher","award":["SUB\/2\/DN\/21\/002\/2226"],"award-info":[{"award-number":["SUB\/2\/DN\/21\/002\/2226"]}],"id":[{"id":"10.13039\/501100005297","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells"],"abstract":"<jats:p>The aim of this study was to evaluate the effect of acute aldosterone (ALDO) administration on the vascular permeability of skin. ALDO was injected intradermally into rats, and vascular permeability was measured. Eplerenone (EPL), a selective mineralocorticoid receptor (MR) antagonist, was used. Skin biopsies were carried out for immunohistochemical (IHC) staining, and polymerase chain reactions were performed to analyze the expression of MR, 11\u03b2-hydroxysteroid dehydrogenase type 2, von Willebrand factor (vWF), vascular endothelial growth factor (VEGF), and zonula occludens 1. Our study showed the presence of MR in the rat skin vasculature for the first time. It was found that ALDO injection resulted in a more than 30% increase in vascular permeability and enhanced the endothelial exocytosis of vWF. The effect of ALDO diminished after EPL administration. An accumulation of vWF and a reduction in VEGF IHC staining were observed following chronic EPL administration. No effect of ALDO or EPL on the mRNA expression of the studied genes or skin structure was observed. The results suggest that ALDO increases vascular permeability in the skin via an MR-dependent mechanism. This effect of ALDO on skin microcirculation may have important therapeutic implications for diseases characterized by increased levels of ALDO and coexisting skin microangiopathy.<\/jats:p>","DOI":"10.3390\/cells11172707","type":"journal-article","created":{"date-parts":[[2022,8,30]],"date-time":"2022-08-30T21:25:18Z","timestamp":1661894718000},"page":"2707","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Aldosterone Increases Vascular Permeability in Rat Skin"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4525-6363","authenticated-orcid":false,"given":"Michal","family":"Aleksiejczuk","sequence":"first","affiliation":[{"name":"Department of Biopharmacy, Medical University of Bialystok, Mickiewicza 2C str., 15-222 Bialystok, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna","family":"Gromotowicz-Poplawska","sequence":"additional","affiliation":[{"name":"Department of Biopharmacy, Medical University of Bialystok, Mickiewicza 2C str., 15-222 Bialystok, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1454-647X","authenticated-orcid":false,"given":"Natalia","family":"Marcinczyk","sequence":"additional","affiliation":[{"name":"Department of Biopharmacy, Medical University of Bialystok, Mickiewicza 2C str., 15-222 Bialystok, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joanna","family":"Stelmaszewska","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical and Biopharmaceutical Analysis, Medical University of Bialystok, Mickiewicza 2D str., 15-222 Bialystok, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Janusz","family":"Dzieciol","sequence":"additional","affiliation":[{"name":"Department of Human Anatomy, Medical University of Bialystok, Mickiewicza 2A str., 15-230 Bialystok, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ewa","family":"Chabielska","sequence":"additional","affiliation":[{"name":"Department of Biopharmacy, Medical University of Bialystok, Mickiewicza 2C str., 15-222 Bialystok, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,8,30]]},"reference":[{"key":"ref_1","first-page":"1334","article-title":"Human skin as target for aldosterone: Coexpression of mineralocorticoid receptors and 11 beta-hydroxysteroid dehydrogenase","volume":"79","author":"Kenouch","year":"1994","journal-title":"J. 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