{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,5]],"date-time":"2026-04-05T06:23:21Z","timestamp":1775370201892,"version":"3.50.1"},"reference-count":53,"publisher":"MDPI AG","issue":"17","license":[{"start":{"date-parts":[[2022,9,2]],"date-time":"2022-09-02T00:00:00Z","timestamp":1662076800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Institutes of Health","award":["UF1NS111695"],"award-info":[{"award-number":["UF1NS111695"]}]},{"name":"National Institutes of Health","award":["R01DC018304"],"award-info":[{"award-number":["R01DC018304"]}]},{"name":"National Institutes of Health","award":["RDC002390"],"award-info":[{"award-number":["RDC002390"]}]},{"name":"National Institutes of Health","award":["R01DC018061"],"award-info":[{"award-number":["R01DC018061"]}]},{"name":"National Institutes of Health","award":["PJT-153150"],"award-info":[{"award-number":["PJT-153150"]}]},{"name":"National Institutes of Health","award":["PJT-173290"],"award-info":[{"award-number":["PJT-173290"]}]},{"name":"Canadian Institutes of Health Research","award":["UF1NS111695"],"award-info":[{"award-number":["UF1NS111695"]}]},{"name":"Canadian Institutes of Health Research","award":["R01DC018304"],"award-info":[{"award-number":["R01DC018304"]}]},{"name":"Canadian Institutes of Health Research","award":["RDC002390"],"award-info":[{"award-number":["RDC002390"]}]},{"name":"Canadian Institutes of Health Research","award":["R01DC018061"],"award-info":[{"award-number":["R01DC018061"]}]},{"name":"Canadian Institutes of Health Research","award":["PJT-153150"],"award-info":[{"award-number":["PJT-153150"]}]},{"name":"Canadian Institutes of Health Research","award":["PJT-173290"],"award-info":[{"award-number":["PJT-173290"]}]},{"name":"Fonds de recherche du Qu\u00e9bec\u2014Sant\u00e9 (FRQS)","award":["UF1NS111695"],"award-info":[{"award-number":["UF1NS111695"]}]},{"name":"Fonds de recherche du Qu\u00e9bec\u2014Sant\u00e9 (FRQS)","award":["R01DC018304"],"award-info":[{"award-number":["R01DC018304"]}]},{"name":"Fonds de recherche du Qu\u00e9bec\u2014Sant\u00e9 (FRQS)","award":["RDC002390"],"award-info":[{"award-number":["RDC002390"]}]},{"name":"Fonds de recherche du Qu\u00e9bec\u2014Sant\u00e9 (FRQS)","award":["R01DC018061"],"award-info":[{"award-number":["R01DC018061"]}]},{"name":"Fonds de recherche du Qu\u00e9bec\u2014Sant\u00e9 (FRQS)","award":["PJT-153150"],"award-info":[{"award-number":["PJT-153150"]}]},{"name":"Fonds de recherche du Qu\u00e9bec\u2014Sant\u00e9 (FRQS)","award":["PJT-173290"],"award-info":[{"award-number":["PJT-173290"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells"],"abstract":"<jats:p>Spinocerebellar Ataxia Type 6 (SCA6) is a mid-life onset neurodegenerative disease characterized by progressive ataxia, dysarthria, and eye movement impairment. This autosomal dominant disease is caused by the expansion of a CAG repeat tract in the CACNA1A gene that encodes the \u03b11A subunit of the P\/Q type voltage-gated Ca2+ channel. Mouse models of SCA6 demonstrate impaired locomotive function and reduced firing precision of cerebellar Purkinje in the anterior vermis. Here, to further assess deficits in other cerebellar-dependent behaviors, we characterized the oculomotor phenotype of a knock-in mouse model with hyper-expanded polyQ repeats (SCA684Q). We found a reduction in the efficacy of the vestibulo-ocular reflex (VOR) and optokinetic reflex (OKR) in SCA6 mutant mice, without a change in phase, compared to their litter-matched controls. Additionally, VOR motor learning was significantly impaired in SCA684Q mice. Given that the floccular lobe of the cerebellum plays a vital role in the generation of OKR and VOR calibration and motor learning, we investigated the firing behavior and morphology of floccular cerebellar Purkinje cells. Overall, we found a reduction in the firing precision of floccular lobe Purkinje cells but no morphological difference between SCA684Q and wild-type mice. Taken together, our findings establish that gaze stabilization and motor learning are impaired in SCA684Q mice and suggest that altered cerebellar output contributes to these deficits.<\/jats:p>","DOI":"10.3390\/cells11172739","type":"journal-article","created":{"date-parts":[[2022,9,5]],"date-time":"2022-09-05T20:48:25Z","timestamp":1662410905000},"page":"2739","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Loss of Flocculus Purkinje Cell Firing Precision Leads to Impaired Gaze Stabilization in a Mouse Model of Spinocerebellar Ataxia Type 6 (SCA6)"],"prefix":"10.3390","volume":"11","author":[{"given":"Hui Ho Vanessa","family":"Chang","sequence":"first","affiliation":[{"name":"Department of Physiology, McGill University, Montreal, QC H3G 1Y6, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7654-0212","authenticated-orcid":false,"given":"Anna A.","family":"Cook","sequence":"additional","affiliation":[{"name":"Department of Biology, McGill University, Montreal, QC H3G 0B1, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6371-6220","authenticated-orcid":false,"given":"Alanna J.","family":"Watt","sequence":"additional","affiliation":[{"name":"Department of Biology, McGill University, Montreal, QC H3G 0B1, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kathleen E.","family":"Cullen","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering, Johns Hopkins University, Baltimore, MD 21205, USA"},{"name":"Department of Otolaryngology-Head and Neck Surgery, School of Medicine, Johns Hopkins University, Baltimore, MD 21205, USA"},{"name":"Department of Neuroscience, School of Medicine, Johns Hopkins University, Baltimore, MD 21205, USA"},{"name":"Kavli Neuroscience Discovery Institute, Johns Hopkins University, Baltimore, MD 21205, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,9,2]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1038\/s41572-019-0074-3","article-title":"Spinocerebellar ataxia","volume":"5","author":"Klockgether","year":"2019","journal-title":"Nat. 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