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Mutations in VLGR1 have been associated with the human Usher syndrome (USH), the most common form of inherited deaf-blindness as well as childhood absence epilepsy. VLGR1 was previously found as membrane\u2013membrane adhesion complexes and focal adhesions. Affinity proteomics revealed that in the interactome of VLGR1, molecules are enriched that are associated with both the ER and mitochondria, as well as mitochondria-associated ER membranes (MAMs), a compartment at the contact sites of both organelles. We confirmed the interaction of VLGR1 with key proteins of MAMs by pull-down assays in vitro complemented by in situ proximity ligation assays in cells. Immunocytochemistry by light and electron microscopy demonstrated the localization of VLGR1 in MAMs. The absence of VLGR1 in tissues and cells derived from VLGR1-deficient mouse models resulted in alterations in the MAM architecture and in the dysregulation of the Ca2+ transient from ER to mitochondria. Our data demonstrate the molecular and functional interaction of VLGR1 with components in MAMs and point to an essential role of VLGR1 in the regulation of Ca2+ homeostasis, one of the key functions of MAMs.<\/jats:p>","DOI":"10.3390\/cells11182790","type":"journal-article","created":{"date-parts":[[2022,9,7]],"date-time":"2022-09-07T20:52:03Z","timestamp":1662583923000},"page":"2790","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":28,"title":["The Adhesion GPCR VLGR1\/ADGRV1 Regulates the Ca2+ Homeostasis at Mitochondria-Associated ER Membranes"],"prefix":"10.3390","volume":"11","author":[{"given":"Jacek","family":"Krzysko","sequence":"first","affiliation":[{"name":"Institute of Molecular Physiology (imP), Molecular Cell Biology, Johannes Gutenberg University Mainz, 55128 Mainz, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Filip","family":"Maciag","sequence":"additional","affiliation":[{"name":"Institute for Developmental Biology and Neurobiology (IDN), Functional Neurobiology, Johannes Gutenberg University Mainz, 55128 Mainz, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Mertens","sequence":"additional","affiliation":[{"name":"Institute of Molecular Physiology (imP), Molecular Cell Biology, Johannes Gutenberg University Mainz, 55128 Mainz, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Baran Enes","family":"G\u00fcler","sequence":"additional","affiliation":[{"name":"Institute of Molecular Physiology (imP), Molecular Cell Biology, Johannes Gutenberg University Mainz, 55128 Mainz, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joshua","family":"Linnert","sequence":"additional","affiliation":[{"name":"Institute of Molecular Physiology (imP), Molecular Cell Biology, Johannes Gutenberg University Mainz, 55128 Mainz, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2693-689X","authenticated-orcid":false,"given":"Karsten","family":"Boldt","sequence":"additional","affiliation":[{"name":"Institute for Ophthalmic Research, University of Tuebingen, 72076 Tuebingen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2209-2113","authenticated-orcid":false,"given":"Marius","family":"Ueffing","sequence":"additional","affiliation":[{"name":"Institute for Ophthalmic Research, University of Tuebingen, 72076 Tuebingen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kerstin","family":"Nagel-Wolfrum","sequence":"additional","affiliation":[{"name":"Institute of Molecular Physiology (imP), Molecular Cell Biology, Johannes Gutenberg University Mainz, 55128 Mainz, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"Heine","sequence":"additional","affiliation":[{"name":"Institute for Developmental Biology and Neurobiology (IDN), Functional Neurobiology, Johannes Gutenberg University Mainz, 55128 Mainz, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4756-5872","authenticated-orcid":false,"given":"Uwe","family":"Wolfrum","sequence":"additional","affiliation":[{"name":"Institute of Molecular Physiology (imP), Molecular Cell Biology, Johannes Gutenberg University Mainz, 55128 Mainz, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,9,7]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"338","DOI":"10.1124\/pr.114.009647","article-title":"International Union of Basic and Clinical Pharmacology. XCIV. Adhesion G protein-coupled receptors","volume":"67","author":"Hamann","year":"2015","journal-title":"Pharmacol. Rev."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1364","DOI":"10.1038\/emboj.2012.26","article-title":"A novel evolutionarily conserved domain of cell-adhesion GPCRs mediates autoproteolysis","volume":"31","author":"Arac","year":"2012","journal-title":"EMBO J."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Knapp, B., Roedig, J., Roedig, H., Krzysko, J., Horn, N., Guler, B.E., Kusuluri, D.K., Yildirim, A., Boldt, K., and Ueffing, M. (2022). Affinity Proteomics Identifies Interaction Partners and Defines Novel Insights into the Function of the Adhesion GPCR VLGR1\/ADGRV1. Molecules, 27.","DOI":"10.3390\/molecules27103108"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1007\/978-1-4419-7913-1_6","article-title":"Studies on the Very Large G Protein-Coupled Receptor: From Initial Discovery to Determining its Role in Sensorineural Deafness in Higher Animals","volume":"Volume 706","author":"McMillan","year":"2010","journal-title":"Adhesion-GPCRs: Structure to Function"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1016\/j.exer.2005.11.010","article-title":"Molecular basis of human Usher syndrome: Deciphering the meshes of the Usher protein network provides insights into the pathomechanisms of the Usher disease","volume":"83","author":"Reiners","year":"2006","journal-title":"Exp. Eye Res."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"537","DOI":"10.1016\/S0896-6273(01)00397-X","article-title":"A novel gene causing a mendelian audiogenic mouse epilepsy","volume":"31","author":"Skradski","year":"2001","journal-title":"Neuron"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"190","DOI":"10.1016\/j.neulet.2009.06.012","article-title":"Deficiency of Vlgr1 resulted in deafness and susceptibility to audiogenic seizures while the degree of hearing impairment was not correlated with seizure severity in C57BL\/6- and 129-backcrossed lines of Vlgr1 knockout mice","volume":"461","author":"Yagi","year":"2009","journal-title":"Neurosci. Lett."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"144","DOI":"10.1111\/nyas.14220","article-title":"Affinity proteomics identifies novel functional modules related to adhesion GPCRs","volume":"1456","author":"Knapp","year":"2019","journal-title":"Ann. N. Y. Acad. Sci."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"102283","DOI":"10.1016\/j.isci.2021.102283","article-title":"Adhesion G protein-coupled receptor VLGR1\/ADGRV1 regulates cell spreading and migration by mechanosensing at focal adhesions","volume":"24","author":"Kusuluri","year":"2021","journal-title":"iScience"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"107","DOI":"10.3389\/fcell.2017.00107","article-title":"Protein Localization at Mitochondria-ER Contact Sites in Basal and Stress Conditions","volume":"5","author":"Ilacqua","year":"2017","journal-title":"Front. Cell Dev. Biol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"7916","DOI":"10.1073\/pnas.1300677110","article-title":"Modulation of the endoplasmic reticulum-mitochondria interface in Alzheimer\u2019s disease and related models","volume":"110","author":"Hedskog","year":"2013","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1007\/978-1-4939-3170-5_11","article-title":"Detection of Isolated Mitochondria-Associated ER Membranes Using the Sigma-1 Receptor","volume":"1376","author":"Lewis","year":"2016","journal-title":"Methods Mol. Biol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"322","DOI":"10.1016\/j.mcn.2004.02.005","article-title":"Loss of the transmembrane and cytoplasmic domains of the very large G-protein-coupled receptor-1 (VLGR1 or Mass1) causes audiogenic seizures in mice","volume":"26","author":"McMillan","year":"2004","journal-title":"Mol. Cell Neurosci."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1093\/hmg\/ddm285","article-title":"A novel Usher protein network at the periciliary reloading point between molecular transport machineries in vertebrate photoreceptor cells","volume":"17","author":"Maerker","year":"2008","journal-title":"Hum. Mol. Genet."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1016\/j.preteyeres.2008.01.003","article-title":"Centrins in retinal photoreceptor cells: Regulators in the connecting cilium","volume":"27","author":"Trojan","year":"2008","journal-title":"Prog. Retin. Eye Res."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"100954","DOI":"10.1016\/j.xpro.2021.100954","article-title":"Isolation and culturing of primary mouse astrocytes for the analysis of focal adhesion dynamics","volume":"2","author":"Guler","year":"2021","journal-title":"STAR Protoc."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"4153","DOI":"10.1038\/ncomms5153","article-title":"Imaging intraorganellar Ca2+ at subcellular resolution using CEPIA","volume":"5","author":"Suzuki","year":"2014","journal-title":"Nat. Commun."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"11491","DOI":"10.1038\/ncomms11491","article-title":"An organelle-specific protein landscape identifies novel diseases and molecular mechanisms","volume":"7","author":"Boldt","year":"2016","journal-title":"Nat. Commun."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1582","DOI":"10.1038\/nprot.2009.151","article-title":"Isolation of mitochondria-associated membranes and mitochondria from animal tissues and cells","volume":"4","author":"Wieckowski","year":"2009","journal-title":"Nat. Protoc."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"733","DOI":"10.1002\/cyto.a.20896","article-title":"Quantifying colocalization by correlation: The Pearson correlation coefficient is superior to the Mander\u2019s overlap coefficient","volume":"77","author":"Adler","year":"2010","journal-title":"Cytometry A"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"88ra55","DOI":"10.1126\/scitranslmed.3002194","article-title":"The Role of Nogo and the Mitochondria-Endoplasmic Reticulum Unit in Pulmonary Hypertension","volume":"3","author":"Sutendra","year":"2011","journal-title":"Sci. Transl. Med."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"5197","DOI":"10.1093\/hmg\/ddu242","article-title":"Disruption of the retinitis pigmentosa 28 gene Fam161a in mice affects photoreceptor ciliary structure and leads to progressive retinal degeneration","volume":"23","author":"Karlstetter","year":"2014","journal-title":"Hum. Mol. Genet."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1083\/jcb.200911095","article-title":"Intraflagellar transport molecules in ciliary and nonciliary cells of the retina","volume":"189","author":"Sedmak","year":"2010","journal-title":"J. Cell Biol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"4228","DOI":"10.1002\/pmic.200700038","article-title":"A novel tandem affinity purification strategy for the efficient isolation and characterisation of native protein complexes","volume":"7","author":"Gloeckner","year":"2007","journal-title":"Proteomics"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"9819","DOI":"10.1021\/ac901335x","article-title":"Software tool for researching annotations of proteins: Open-source protein annotation software with data visualization","volume":"81","author":"Bhatia","year":"2009","journal-title":"Anal. Chem."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1091","DOI":"10.1093\/bioinformatics\/btp101","article-title":"ClueGO: A Cytoscape plug-in to decipher functionally grouped gene ontology and pathway annotation networks","volume":"25","author":"Bindea","year":"2009","journal-title":"Bioinformatics"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1326","DOI":"10.15252\/embr.201541726","article-title":"ALS\/FTD-associated FUS activates GSK-3beta to disrupt the VAPB-PTPIP51 interaction and ER-mitochondria associations","volume":"17","author":"Stoica","year":"2016","journal-title":"EMBO Rep."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1007\/978-3-319-55858-5_13","article-title":"Mitochondrial VDAC, the Na+\/Ca2+ Exchanger, and the Ca2+ Uniporter in Ca2+ Dynamics and Signaling","volume":"981","author":"De","year":"2017","journal-title":"Adv. Exp. Med. Biol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1016\/S0003-9861(02)00247-3","article-title":"Rat liver acyl-CoA synthetase 4 is a peripheral-membrane protein located in two distinct subcellular organelles, peroxisomes, and mitochondrial-associated membrane","volume":"404","author":"Lewin","year":"2002","journal-title":"Arch. Biochem. Biophys."},{"key":"ref_30","first-page":"365","article-title":"An alternative import pathway of AIF to the mitochondria","volume":"29","author":"Chiang","year":"2012","journal-title":"Int. J. Mol. Med."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"3553","DOI":"10.1242\/jcs.03486","article-title":"Reversible interactions between smooth domains of the endoplasmic reticulum and mitochondria are regulated by physiological cytosolic Ca2+ levels","volume":"120","author":"Goetz","year":"2007","journal-title":"J. Cell Sci."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"556","DOI":"10.1038\/emboj.2010.346","article-title":"Fis1 and Bap31 bridge the mitochondria-ER interface to establish a platform for apoptosis induction","volume":"30","author":"Iwasawa","year":"2011","journal-title":"EMBO J."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"E1237","DOI":"10.1152\/ajpendo.00594.2005","article-title":"Colocalization of MCT1, CD147, and LDH in mitochondrial inner membrane of L6 muscle cells: Evidence of a mitochondrial lactate oxidation complex","volume":"290","author":"Hashimoto","year":"2006","journal-title":"Am. J. Physiol. Endocrinol. Metab."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"2777","DOI":"10.1091\/mbc.e07-10-0995","article-title":"The subcellular distribution of calnexin is mediated by PACS-2","volume":"19","author":"Myhill","year":"2008","journal-title":"Mol. Biol. Cell"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"4770","DOI":"10.1093\/hmg\/ddu193","article-title":"Cisd2 modulates the differentiation and functioning of adipocytes by regulating intracellular Ca2+ homeostasis","volume":"23","author":"Wang","year":"2014","journal-title":"Hum. Mol. Genet."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"3149","DOI":"10.1242\/jcs.03060","article-title":"Erlin-1 and erlin-2 are novel members of the prohibitin family of proteins that define lipid-raft-like domains of the ER","volume":"119","author":"Browman","year":"2006","journal-title":"J. Cell Sci."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"619","DOI":"10.1007\/s12192-010-0174-1","article-title":"Ero1alpha requires oxidizing and normoxic conditions to localize to the mitochondria-associated membrane (MAM)","volume":"15","author":"Gilady","year":"2010","journal-title":"Cell Stress Chaperones"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"527","DOI":"10.1042\/BJ20031819","article-title":"Subcellular compartmentalization of ceramide metabolism: MAM (mitochondria-associated membrane) and\/or mitochondria?","volume":"382","author":"Bionda","year":"2004","journal-title":"Biochem. J."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"596","DOI":"10.1016\/j.cell.2007.08.036","article-title":"Sigma-1 receptor chaperones at the ER-mitochondrion interface regulate Ca2+ signaling and cell survival","volume":"131","author":"Hayashi","year":"2007","journal-title":"Cell"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"901","DOI":"10.1083\/jcb.200608073","article-title":"Chaperone-mediated coupling of endoplasmic reticulum and mitochondrial Ca2+ channels","volume":"175","author":"Szabadkai","year":"2006","journal-title":"J. Cell Biol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"632","DOI":"10.1016\/S1367-5931(00)00151-4","article-title":"Dissecting and manipulating the pathway for glycosylphos-phatidylinositol-anchor biosynthesis","volume":"4","author":"Kinoshita","year":"2000","journal-title":"Curr. Opin. Chem. Biol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"27494","DOI":"10.1016\/S0021-9258(18)47012-3","article-title":"A unique mitochondria-associated membrane fraction from rat liver has a high capacity for lipid synthesis and contains pre-Golgi secretory proteins including nascent lipoproteins","volume":"269","author":"Rusinol","year":"1994","journal-title":"J. Biol. Chem."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"14590","DOI":"10.1073\/pnas.1110133108","article-title":"Mitochondrial-associated endoplasmic reticulum membranes (MAM) form innate immune synapses and are targeted by hepatitis C virus","volume":"108","author":"Horner","year":"2011","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"900","DOI":"10.1038\/nchembio.467","article-title":"Inhibitors of protein disulfide isomerase suppress apoptosis induced by misfolded proteins","volume":"6","author":"Hoffstrom","year":"2010","journal-title":"Nat. Chem. Biol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"1810","DOI":"10.2353\/ajpath.2009.090219","article-title":"Presenilins are enriched in endoplasmic reticulum membranes associated with mitochondria","volume":"175","author":"Boldogh","year":"2009","journal-title":"Am. J. Pathol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"34534","DOI":"10.1074\/jbc.M002865200","article-title":"Phosphatidylserine synthase-1 and -2 are localized to mitochondria-associated membranes","volume":"275","author":"Stone","year":"2000","journal-title":"J. Biol. Chem."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"20728","DOI":"10.1073\/pnas.0808953105","article-title":"Bidirectional Ca2+-dependent control of mitochondrial dynamics by the Miro GTPase","volume":"105","author":"Saotome","year":"2008","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"3109","DOI":"10.1093\/brain\/awu287","article-title":"Membrane-shaping disorders: A common pathway in axon degeneration","volume":"137","author":"Hubner","year":"2014","journal-title":"Brain"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"1928","DOI":"10.1194\/jlr.M600172-JLR200","article-title":"Colocalization of SCD1 and DGAT2: Implying preference for endogenous monounsaturated fatty acids in triglyceride synthesis","volume":"47","author":"Man","year":"2006","journal-title":"J. Lipid Res."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1007\/s12031-007-0038-z","article-title":"The fatty acid transport protein (FATP) family: Very long chain acyl-CoA synthetases or solute carriers?","volume":"33","author":"Jia","year":"2007","journal-title":"J. Mol. Neurosci."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1016\/j.ceca.2010.12.004","article-title":"Voltage-dependent anion channel 2 modulates resting Ca2+ sparks, but not action potential-induced Ca2+ signaling in cardiac myocytes","volume":"49","author":"Subedi","year":"2011","journal-title":"Cell Calcium"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"364","DOI":"10.1038\/s41419-018-0406-3","article-title":"Wolfram syndrome: MAMs\u2019 connection?","volume":"9","author":"Delprat","year":"2018","journal-title":"Cell Death Dis."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"272","DOI":"10.1016\/j.bbrc.2012.10.138","article-title":"Long-chain acyl-CoA synthetase 4 is regulated by phosphorylation","volume":"430","author":"Smith","year":"2013","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"2337","DOI":"10.1128\/MCB.21.7.2337-2348.2001","article-title":"Protein import channel of the outer mitochondrial membrane: A highly stable Tom40-Tom22 core structure differentially interacts with preproteins, small tom proteins, and import receptors","volume":"21","author":"Meisinger","year":"2001","journal-title":"Mol. Cell Biol."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"27351","DOI":"10.1038\/srep27351","article-title":"Interplay between hepatic mitochondria-associated membranes, lipid metabolism and caveolin-1 in mice","volume":"6","author":"Bosch","year":"2016","journal-title":"Sci. Rep."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"1287","DOI":"10.1083\/jcb.153.6.1287","article-title":"Subdomain-specific localization of CLIMP-63 (p63) in the endoplasmic reticulum is mediated by its luminal alpha-helical segment","volume":"153","author":"Klopfenstein","year":"2001","journal-title":"J. Cell Biol."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"1417","DOI":"10.1038\/cdd.2016.52","article-title":"The coming of age of the mitochondria-ER contact: A matter of thickness","volume":"23","author":"Giacomello","year":"2016","journal-title":"Cell Death Differ."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"1096","DOI":"10.1016\/j.bbrc.2016.07.056","article-title":"(Neuro)degenerated Mitochondria-ER contacts","volume":"483","author":"Martinvalet","year":"2017","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"106909","DOI":"10.1016\/j.yebeh.2020.106909","article-title":"The cerebellum and epilepsy","volume":"121","author":"Streng","year":"2021","journal-title":"Epilepsy Behav."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"eaat7397","DOI":"10.1126\/scisignal.aat7397","article-title":"Polycystin 2 regulates mitochondrial Ca2+ signaling, bioenergetics, and dynamics through mitofusin 2","volume":"12","author":"Kuo","year":"2019","journal-title":"Sci. Signal."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"623","DOI":"10.1126\/science.aan6009","article-title":"ER-mitochondria tethering by PDZD8 regulates Ca2+ dynamics in mammalian neurons","volume":"358","author":"Hirabayashi","year":"2017","journal-title":"Science"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"2961","DOI":"10.1523\/JNEUROSCI.15-04-02961.1995","article-title":"ATP causes release of intracellular Ca2+ via the phospholipase C beta\/IP3 pathway in astrocytes from the dorsal spinal cord","volume":"15","author":"Salter","year":"1995","journal-title":"J. Neurosci."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"C577","DOI":"10.1152\/ajpcell.1993.265.3.C577","article-title":"Signal transduction via P2-purinergic receptors for extracellular ATP and other nucleotides","volume":"265","author":"Dubyak","year":"1993","journal-title":"Am. J. Physiol."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"1427","DOI":"10.1242\/dev.012922","article-title":"A core cochlear phenotype in USH1 mouse mutants implicates fibrous links of the hair bundle in its cohesion, orientation and differential growth","volume":"135","author":"Lefevre","year":"2008","journal-title":"Development"},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"eaan5835","DOI":"10.1126\/science.aan5835","article-title":"Here, there, and everywhere: The importance of ER membrane contact sites","volume":"361","author":"Wu","year":"2018","journal-title":"Science"},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"1763","DOI":"10.1126\/science.280.5370.1763","article-title":"Close contacts with the endoplasmic reticulum as determinants of mitochondrial Ca2+ responses","volume":"280","author":"Rizzuto","year":"1998","journal-title":"Science"},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"442","DOI":"10.1016\/j.bbamcr.2006.04.002","article-title":"Mitochondrial dynamics and Ca2+ signaling","volume":"1763","author":"Szabadkai","year":"2006","journal-title":"Biochim. Biophys. Acta"},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"br1","DOI":"10.1091\/mbc.E21-01-0042","article-title":"The receptor subunit Tom20 is dynamically associated with the TOM complex in mitochondria of human cells","volume":"32","author":"Bhagawati","year":"2021","journal-title":"Mol. Biol. Cell"},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"E8844","DOI":"10.1073\/pnas.1721136115","article-title":"Altered ER-mitochondria contact impacts mitochondria calcium homeostasis and contributes to neurodegeneration in vivo in disease models","volume":"115","author":"Lee","year":"2018","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"146","DOI":"10.1016\/j.tins.2016.01.008","article-title":"There\u2019s Something Wrong with my MAM; the ER-Mitochondria Axis and Neurodegenerative Diseases","volume":"39","author":"Paillusson","year":"2016","journal-title":"Trends Neurosci."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"2291","DOI":"10.15252\/embj.201591565","article-title":"Triggered Ca2+ influx is required for extended synaptotagmin 1-induced ER-plasma membrane tethering","volume":"34","author":"Lu","year":"2015","journal-title":"EMBO J."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"899","DOI":"10.1042\/bj1760899","article-title":"Calcium ions and the regulation of NAD+-linked isocitrate dehydrogenase from the mitochondria of rat heart and other tissues","volume":"176","author":"Denton","year":"1978","journal-title":"Biochem. J."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"270","DOI":"10.1016\/j.cell.2010.06.007","article-title":"Essential regulation of cell bioenergetics by constitutive InsP3 receptor Ca2+ transfer to mitochondria","volume":"142","author":"Cardenas","year":"2010","journal-title":"Cell"},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"14670","DOI":"10.1073\/pnas.0903563106","article-title":"Mitochondrial bioenergetic deficit precedes Alzheimer\u2019s pathology in female mouse model of Alzheimer\u2019s disease","volume":"106","author":"Yao","year":"2009","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1007\/978-1-4615-0067-4_34","article-title":"Energy depletion hypothesis for retinitis pigmentosa","volume":"533","author":"Eckmiller","year":"2003","journal-title":"Adv. Exp. Med. Biol."}],"container-title":["Cells"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2073-4409\/11\/18\/2790\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T00:24:51Z","timestamp":1760142291000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2073-4409\/11\/18\/2790"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,9,7]]},"references-count":75,"journal-issue":{"issue":"18","published-online":{"date-parts":[[2022,9]]}},"alternative-id":["cells11182790"],"URL":"https:\/\/doi.org\/10.3390\/cells11182790","relation":{},"ISSN":["2073-4409"],"issn-type":[{"value":"2073-4409","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,9,7]]}}}