{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T01:19:38Z","timestamp":1789089578523,"version":"build-2803163510"},"reference-count":45,"publisher":"MDPI AG","issue":"18","license":[{"start":{"date-parts":[[2022,9,7]],"date-time":"2022-09-07T00:00:00Z","timestamp":1662508800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea (NRF)","doi-asserted-by":"publisher","award":["NRF-2020R1C1C1009913"],"award-info":[{"award-number":["NRF-2020R1C1C1009913"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea (NRF)","doi-asserted-by":"publisher","award":["NRF-2021R1A2B5B01002047"],"award-info":[{"award-number":["NRF-2021R1A2B5B01002047"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea (NRF)","doi-asserted-by":"publisher","award":["NRF-2022R1C1C2004305"],"award-info":[{"award-number":["NRF-2022R1C1C2004305"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells"],"abstract":"<jats:p>Premature ovarian insufficiency (POI) is a typical disorder of amenorrhea that lasts for a minimum of four months in women &lt; 40 years old and is typically characterized by reduced estrogen levels and elevated serum concentrations of follicle-stimulating hormone. We collected urine samples from two participant cohorts from Gil Hospital of Gachon University (Incheon, Korea): a sequencing cohort of 19 participants (seven patients with POI (POI patients without Turner syndrome), seven patients with Turner syndrome (POI patients with Turner syndrome), and five control individuals (age-matched controls with confirmed ovarian sufficiency)) and a validation cohort of 46 participants (15 patients with POI, 11 patients with Turner syndrome, and 20 control individuals). Among differentially expressed miRNAs, hsa-miR-4516 was significantly upregulated in patients with POI in both cohorts, independent of the presence of Turner syndrome. Moreover, the upregulation of miR-4516 was confirmed in the ovary\u2014but not in the uterus\u2014of a cyclophosphamide and busulfan-induced POI mouse model. This was accompanied by a decrease in STAT3 protein level, a predicted target of miR-4516, via miRTarBase2020. Our study provides compelling evidence that miR-4516 is highly expressed in patients with POI and POI mouse models, suggesting that miR-4516 is a diagnostic marker of POI.<\/jats:p>","DOI":"10.3390\/cells11182797","type":"journal-article","created":{"date-parts":[[2022,9,7]],"date-time":"2022-09-07T20:52:03Z","timestamp":1662583923000},"page":"2797","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["MicroRNA-4516 in Urinary Exosomes as a Biomarker of Premature Ovarian Insufficiency"],"prefix":"10.3390","volume":"11","author":[{"given":"Zobia","family":"Umair","sequence":"first","affiliation":[{"name":"Department of Molecular Medicine, School of Medicine, Gachon University, Incheon 21999, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mi-Ock","family":"Baek","sequence":"additional","affiliation":[{"name":"Department of Molecular Medicine, School of Medicine, Gachon University, Incheon 21999, Korea"},{"name":"Lee Gil Ya Cancer and Diabetes Institute, Gachon University, Incheon 21999, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jisue","family":"Song","sequence":"additional","affiliation":[{"name":"Gachon University Gil Medical Center, Department of Obstetrics and Gynecology, College of Medicine, Gachon University, Incheon 21565, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seona","family":"An","sequence":"additional","affiliation":[{"name":"Gachon University Gil Medical Center, Department of Obstetrics and Gynecology, College of Medicine, Gachon University, Incheon 21565, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seung Joo","family":"Chon","sequence":"additional","affiliation":[{"name":"Gachon University Gil Medical Center, Department of Obstetrics and Gynecology, College of Medicine, Gachon University, Incheon 21565, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0114-1142","authenticated-orcid":false,"given":"Mee-Sup","family":"Yoon","sequence":"additional","affiliation":[{"name":"Department of Molecular Medicine, School of Medicine, Gachon University, Incheon 21999, Korea"},{"name":"Lee Gil Ya Cancer and Diabetes Institute, Gachon University, Incheon 21999, Korea"},{"name":"Department of Health Sciences and Technology, GAIHST, Gachon University, Incheon 21999, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,9,7]]},"reference":[{"key":"ref_1","first-page":"105","article-title":"Premature ovarian insufficiency-aetiopathology, epidemiology, and diagnostic evaluation","volume":"17","author":"Rudnicka","year":"2018","journal-title":"Prz. 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