{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T23:53:17Z","timestamp":1778889197707,"version":"3.51.4"},"reference-count":36,"publisher":"MDPI AG","issue":"18","license":[{"start":{"date-parts":[[2022,9,12]],"date-time":"2022-09-12T00:00:00Z","timestamp":1662940800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Research Foundation of Korea","award":["2020R1A2C2003523"],"award-info":[{"award-number":["2020R1A2C2003523"]}]},{"name":"National Research Foundation of Korea","award":["2020R1I1A1A01073696"],"award-info":[{"award-number":["2020R1I1A1A01073696"]}]},{"name":"NRF of Korea","award":["2020R1A2C2003523"],"award-info":[{"award-number":["2020R1A2C2003523"]}]},{"name":"NRF of Korea","award":["2020R1I1A1A01073696"],"award-info":[{"award-number":["2020R1I1A1A01073696"]}]},{"name":"ORGASIS Corporation","award":["2020R1A2C2003523"],"award-info":[{"award-number":["2020R1A2C2003523"]}]},{"name":"ORGASIS Corporation","award":["2020R1I1A1A01073696"],"award-info":[{"award-number":["2020R1I1A1A01073696"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells"],"abstract":"<jats:p>Although autophagy regulates the quality and quantity of cellular compartments, the regulatory mechanisms underlying peroxisomal autophagy (pexophagy) remain largely unknown. In this study, we identified several BRD4 inhibitors, including molibresib, a novel pexophagy inducer, via chemical library screening. Treatment with molibresib promotes loss of peroxisomes selectively, but not mitochondria, ER, or Golgi apparatus in HeLa cells. Consistently, depletion of BRD4 expression also induced pexophagy in RPE cells. In addition, the inhibition of BRD4 by molibresib increased autophagic degradation of peroxisome ATG7-dependency. We further found that molibresib produced reactive oxygen species (ROS), which potentiates ATM activation. Inhibition of ROS or ATM suppressed the loss of peroxisomes in molibresib-treated cells. Taken together, our data suggest that inhibition of BRD4 promotes pexophagy by increasing ROS and ATM activation.<\/jats:p>","DOI":"10.3390\/cells11182839","type":"journal-article","created":{"date-parts":[[2022,9,12]],"date-time":"2022-09-12T20:52:25Z","timestamp":1663015945000},"page":"2839","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Inhibition of BRD4 Promotes Pexophagy by Increasing ROS and ATM Activation"],"prefix":"10.3390","volume":"11","author":[{"given":"Yong Hwan","family":"Kim","sequence":"first","affiliation":[{"name":"BK21 FOUR KNU Creative BioResearch Group, School of Life Sciences, Kyungpook National University, Daegu 41566, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Doo Sin","family":"Jo","sequence":"additional","affiliation":[{"name":"BK21 FOUR KNU Creative BioResearch Group, School of Life Sciences, Kyungpook National University, Daegu 41566, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Na Yeon","family":"Park","sequence":"additional","affiliation":[{"name":"BK21 FOUR KNU Creative BioResearch Group, School of Life Sciences, Kyungpook National University, Daegu 41566, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ji-Eun","family":"Bae","sequence":"additional","affiliation":[{"name":"Brain Science and Engineering Institute, Kyungpook National University, Daegu 41566, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joon Bum","family":"Kim","sequence":"additional","affiliation":[{"name":"BK21 FOUR KNU Creative BioResearch Group, School of Life Sciences, Kyungpook National University, Daegu 41566, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ha Jung","family":"Lee","sequence":"additional","affiliation":[{"name":"BK21 FOUR KNU Creative BioResearch Group, School of Life Sciences, Kyungpook National University, Daegu 41566, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"So Hyun","family":"Kim","sequence":"additional","affiliation":[{"name":"BK21 FOUR KNU Creative BioResearch Group, School of Life Sciences, Kyungpook National University, Daegu 41566, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Seong Hyun","family":"Kim","sequence":"additional","affiliation":[{"name":"BK21 FOUR KNU Creative BioResearch Group, School of Life Sciences, Kyungpook National University, Daegu 41566, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sunwoo","family":"Lee","sequence":"additional","affiliation":[{"name":"BK21 FOUR KNU Creative BioResearch Group, School of Life Sciences, Kyungpook National University, Daegu 41566, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mikyung","family":"Son","sequence":"additional","affiliation":[{"name":"Orgasis Corp., Suwon 16229, Gyeonggi-do, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kyuhee","family":"Park","sequence":"additional","affiliation":[{"name":"Bio-Center, Gyeonggido Business & Science Accelerator, Suwon 16229, Gyeonggi-do, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kwiwan","family":"Jeong","sequence":"additional","affiliation":[{"name":"Bio-Center, Gyeonggido Business & Science Accelerator, Suwon 16229, Gyeonggi-do, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eunbyul","family":"Yeom","sequence":"additional","affiliation":[{"name":"BK21 FOUR KNU Creative BioResearch Group, School of Life Sciences, Kyungpook National University, Daegu 41566, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dong-Hyung","family":"Cho","sequence":"additional","affiliation":[{"name":"BK21 FOUR KNU Creative BioResearch Group, School of Life Sciences, Kyungpook National University, Daegu 41566, Korea"},{"name":"Orgasis Corp., Suwon 16229, Gyeonggi-do, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,9,12]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1486","DOI":"10.1038\/s12276-020-00503-9","article-title":"Peroxisome quality control and dysregulated lipid metabolism in neurodegenerative diseases","volume":"52","author":"Jo","year":"2020","journal-title":"Exp. 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