{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,23]],"date-time":"2026-01-23T23:23:12Z","timestamp":1769210592898,"version":"3.49.0"},"reference-count":30,"publisher":"MDPI AG","issue":"18","license":[{"start":{"date-parts":[[2022,9,18]],"date-time":"2022-09-18T00:00:00Z","timestamp":1663459200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Swedish Heart and Lung Foundation","award":["20200839"],"award-info":[{"award-number":["20200839"]}]},{"name":"Swedish Heart and Lung Foundation","award":["2017-03064"],"award-info":[{"award-number":["2017-03064"]}]},{"name":"The Swedish Research Council","award":["20200839"],"award-info":[{"award-number":["20200839"]}]},{"name":"The Swedish Research Council","award":["2017-03064"],"award-info":[{"award-number":["2017-03064"]}]},{"name":"The Swedish Asthma and Allergy Associations Research Foundation","award":["20200839"],"award-info":[{"award-number":["20200839"]}]},{"name":"The Swedish Asthma and Allergy Associations Research Foundation","award":["2017-03064"],"award-info":[{"award-number":["2017-03064"]}]},{"name":"The Crafoord Foundation","award":["20200839"],"award-info":[{"award-number":["20200839"]}]},{"name":"The Crafoord Foundation","award":["2017-03064"],"award-info":[{"award-number":["2017-03064"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells"],"abstract":"<jats:p>Tissue damage, epithelial alterations, and intraepithelial presence of mast cells (MCs) are characteristics of asthma pathogenesis. Increased alveolar infiltration of MC populations has also been identified as a feature of asthma and other chronic respiratory diseases. The asthma associated receptor, urokinase plasminogen activator receptor (uPAR), has been shown to regulate bronchial epithelial repair responses. However, the impact of MC tryptase and chymase on functional properties and expression of uPAR in alveolar epithelial cells have not been fully investigated. Alveolar epithelial cell migration and wound healing were investigated using holographic live cell imaging of A549 cells in a wound scratch model post stimulation with tryptase or chymase. The expression of uPAR was investigated on the protein and gene level from cellular supernatants and in bronchoalveolar lavage fluid fractions from allergic asthmatics. We found that tryptase improved wound healing capacity, cellular migration and membrane bound uPAR expression. Chymase reduced gap closure capacity, cellular migration and membrane bound uPAR expression but increased soluble uPAR release. Our data suggest a dual regulatory response from the MC proteases in events related to uPAR expression and wound healing which could be important features in asthmatic disease.<\/jats:p>","DOI":"10.3390\/cells11182916","type":"journal-article","created":{"date-parts":[[2022,9,18]],"date-time":"2022-09-18T22:12:43Z","timestamp":1663539163000},"page":"2916","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Mast Cell Proteases Promote Diverse Effects on the Plasminogen Activation System and Wound Healing in A549 Alveolar Epithelial Cells"],"prefix":"10.3390","volume":"11","author":[{"given":"Sofia","family":"Mogren","sequence":"first","affiliation":[{"name":"Department of Experimental Medical Science, BMC, Lund University, 222 42 Lund, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3728-1729","authenticated-orcid":false,"given":"Frida","family":"Berlin","sequence":"additional","affiliation":[{"name":"Department of Experimental Medical Science, BMC, Lund University, 222 42 Lund, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lykke","family":"Eskilsson","sequence":"additional","affiliation":[{"name":"Department of Experimental Medical Science, BMC, Lund University, 222 42 Lund, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicole","family":"Van Der Burg","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences, BMC, Lund University, 222 42 Lund, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ellen","family":"Tufvesson","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences, BMC, Lund University, 222 42 Lund, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6450-7300","authenticated-orcid":false,"given":"Cecilia K.","family":"Andersson","sequence":"additional","affiliation":[{"name":"Department of Experimental Medical Science, BMC, Lund University, 222 42 Lund, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,9,18]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1111\/all.14881","article-title":"The ingenious mast cell: Contemporary insights into mast cell behavior and function","volume":"77","author":"Dahlin","year":"2021","journal-title":"Allergy"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"905","DOI":"10.1016\/j.jaci.2011.01.022","article-title":"Mast cell-associated alveolar inflammation in patients with atopic uncontrolled asthma","volume":"127","author":"Andersson","year":"2011","journal-title":"J. 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