{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T20:36:54Z","timestamp":1782506214699,"version":"3.54.5"},"reference-count":43,"publisher":"MDPI AG","issue":"19","license":[{"start":{"date-parts":[[2022,9,21]],"date-time":"2022-09-21T00:00:00Z","timestamp":1663718400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31872310"],"award-info":[{"award-number":["31872310"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31802022"],"award-info":[{"award-number":["31802022"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells"],"abstract":"<jats:p>Joint disorders have become a global health issue with the growth of the aging population. Screening small active molecules targeting chondrogenic differentiation of bone marrow-derived stem cells (BMSCs) is of urgency. In this study, microfracture was employed to create a regenerative niche in rabbits (n = 9). Cartilage samples were collected four weeks post-surgery. Microfracture-caused morphological (n = 3) and metabolic (n = 6) changes were detected. Non-targeted metabolomic analysis revealed that there were 96 differentially expressed metabolites (DEMs) enriched in 70 pathways involved in anti-inflammation, lipid metabolism, signaling transduction, etc. Among the metabolites, docosapentaenoic acid 22n-3 (DPA) and ursodeoxycholic acid (UDCA) functionally facilitated cartilage defect healing, i.e., increasing the vitality and adaptation of the BMSCs, chondrogenic differentiation, and chondrocyte functionality. Our findings firstly reveal the differences in metabolomic activities between the normal and regenerated cartilages and provide a list of endogenous biomolecules potentially involved in the biochemical-niche fate control for chondrogenic differentiation of BMSCs. Ultimately, the biomolecules may serve as anti-aging supplements for chondrocyte renewal or as drug candidates for cartilage regenerative medicine.<\/jats:p>","DOI":"10.3390\/cells11192951","type":"journal-article","created":{"date-parts":[[2022,9,21]],"date-time":"2022-09-21T21:22:23Z","timestamp":1663795343000},"page":"2951","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Cartilaginous Metabolomics Reveals the Biochemical-Niche Fate Control of Bone Marrow-Derived Stem Cells"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5136-3790","authenticated-orcid":false,"given":"Haining","family":"Peng","sequence":"first","affiliation":[{"name":"Department of Sports Medicine, The Affiliated Hospital of Qingdao University, Qingdao 266000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Sports Medicine, The Affiliated Hospital of Qingdao University, Qingdao 266000, China"},{"name":"Institute of Sports Medicine and Rehabilitation, Qingdao University, Qingdao 266000, China"},{"name":"Shandong Institute of Traumatic Orthopedics, Medical Research Center, The Affiliated Hospital of Qingdao University, Qingdao 266590, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhongkai","family":"Ren","sequence":"additional","affiliation":[{"name":"Department of Sports Medicine, The Affiliated Hospital of Qingdao University, Qingdao 266000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ziran","family":"Wei","sequence":"additional","affiliation":[{"name":"Department of Sports Medicine, The Affiliated Hospital of Qingdao University, Qingdao 266000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Renjie","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Sports Medicine, The Affiliated Hospital of Qingdao University, Qingdao 266000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yingze","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Orthopedics, The Third Hospital of Hebei Medical University, Shijiazhuang 050051, China"},{"name":"Department of Orthopedics, The Affiliated Hospital of Qingdao University, Qingdao 266000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3617-8911","authenticated-orcid":false,"given":"Xiaohong","family":"Huang","sequence":"additional","affiliation":[{"name":"Shandong Institute of Traumatic Orthopedics, Medical Research Center, The Affiliated Hospital of Qingdao University, Qingdao 266590, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tengbo","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Sports Medicine, The Affiliated Hospital of Qingdao University, Qingdao 266000, China"},{"name":"Institute of Sports Medicine and Rehabilitation, Qingdao University, Qingdao 266000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,9,21]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Roseti, L., Desando, G., Cavallo, C., Petretta, M., and Grigolo, B. 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