{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,17]],"date-time":"2026-07-17T16:44:36Z","timestamp":1784306676481,"version":"3.55.0"},"reference-count":76,"publisher":"MDPI AG","issue":"19","license":[{"start":{"date-parts":[[2022,10,10]],"date-time":"2022-10-10T00:00:00Z","timestamp":1665360000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003725","name":"Ministry of Science and ICT","doi-asserted-by":"publisher","award":["2021R1A2C2009294"],"award-info":[{"award-number":["2021R1A2C2009294"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003725","name":"Ministry of Science and ICT","doi-asserted-by":"publisher","award":["2022R1I1A1A01065412"],"award-info":[{"award-number":["2022R1I1A1A01065412"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003725","name":"Ministry of Science and ICT","doi-asserted-by":"publisher","award":["2021H1D3A2A02040098"],"award-info":[{"award-number":["2021H1D3A2A02040098"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003725","name":"Brain Pool program","doi-asserted-by":"publisher","award":["2021R1A2C2009294"],"award-info":[{"award-number":["2021R1A2C2009294"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003725","name":"Brain Pool program","doi-asserted-by":"publisher","award":["2022R1I1A1A01065412"],"award-info":[{"award-number":["2022R1I1A1A01065412"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003725","name":"Brain Pool program","doi-asserted-by":"publisher","award":["2021H1D3A2A02040098"],"award-info":[{"award-number":["2021H1D3A2A02040098"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells"],"abstract":"<jats:p>Extracellular vesicles (EVs) are nanosized vesicles that act as snapshots of cellular components and mediate cellular communications, but they may contain cargo contents with undesired effects. We developed a model to improve the effects of endometrium-derived EVs (Endo-EVs) on the porcine embryo attachment in feeder-free culture conditions. Endo-EVs cargo contents were analyzed using conventional and real-time PCR for micro-RNAs, messenger RNAs, and proteomics. Porcine embryos were generated by parthenogenetic electric activation in feeder-free culture conditions supplemented with or without Endo-EVs. The cellular uptake of Endo-EVs was confirmed using the lipophilic dye PKH26. Endo-EVs cargo contained miR-100, miR-132, and miR-155, together with the mRNAs of porcine endogenous retrovirus (PERV) and \u03b2-catenin. Targeting PERV with CRISPR\/Cas9 resulted in reduced expression of PERV mRNA transcripts and increased miR-155 in the Endo-EVs, and supplementing these in embryos reduced embryo attachment. Supplementing the medium containing Endo-EVs with miR-155 inhibitor significantly improved the embryo attachment with a few outgrowths, while supplementing with Rho-kinase inhibitor (RI, Y-27632) dramatically improved both embryo attachment and outgrowths. Moreover, the expression of miR-100, miR-132, and the mRNA transcripts of BCL2, zinc finger E-box-binding homeobox 1, \u03b2-catenin, interferon-\u03b3, protein tyrosine phosphatase non-receptor type 1, PERV, and cyclin-dependent kinase 2 were all increased in embryos supplemented with Endo-EVs + RI compared to those in the control group. Endo-EVs + RI reduced apoptosis and increased the expression of OCT4 and CDX2 and the cell number of embryonic outgrowths. We examined the individual and combined effects of RI compared to those of the miR-155 mimic and found that RI can alleviate the negative effects of the miR-155 mimic on embryo attachment and outgrowths. EVs can improve embryo attachment and the unwanted effects of the de trop cargo contents (miR-155) can be alleviated through anti-apoptotic molecules such as the ROCK inhibitor.<\/jats:p>","DOI":"10.3390\/cells11193178","type":"journal-article","created":{"date-parts":[[2022,10,10]],"date-time":"2022-10-10T21:07:11Z","timestamp":1665436031000},"page":"3178","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["ROCK Inhibitor (Y-27632) Abolishes the Negative Impacts of miR-155 in the Endometrium-Derived Extracellular Vesicles and Supports Embryo Attachment"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7633-730X","authenticated-orcid":false,"given":"Islam M.","family":"Saadeldin","sequence":"first","affiliation":[{"name":"Laboratory of Theriogenology, College of Veterinary Medicine, Chungnam National University, Daejeon 34134, Korea"},{"name":"Research Institute of Veterinary Medicine, Chungnam National University, Daejeon 34134, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9901-5875","authenticated-orcid":false,"given":"Bereket Molla","family":"Tanga","sequence":"additional","affiliation":[{"name":"Laboratory of Theriogenology, College of Veterinary Medicine, Chungnam National University, Daejeon 34134, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seonggyu","family":"Bang","sequence":"additional","affiliation":[{"name":"Laboratory of Theriogenology, College of Veterinary Medicine, Chungnam National University, Daejeon 34134, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chaerim","family":"Seo","sequence":"additional","affiliation":[{"name":"Laboratory of Theriogenology, College of Veterinary Medicine, Chungnam National University, Daejeon 34134, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6399-6472","authenticated-orcid":false,"given":"Okjae","family":"Koo","sequence":"additional","affiliation":[{"name":"Toolgen Inc., Seoul 08501, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sung Ho","family":"Yun","sequence":"additional","affiliation":[{"name":"Korea Basic Science Institute (KBSI), Ochang 28119, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seung Il","family":"Kim","sequence":"additional","affiliation":[{"name":"Korea Basic Science Institute (KBSI), Ochang 28119, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1265-3821","authenticated-orcid":false,"given":"Sanghoon","family":"Lee","sequence":"additional","affiliation":[{"name":"Laboratory of Theriogenology, College of Veterinary Medicine, Chungnam National University, Daejeon 34134, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8431-0457","authenticated-orcid":false,"given":"Jongki","family":"Cho","sequence":"additional","affiliation":[{"name":"Laboratory of Theriogenology, College of Veterinary Medicine, Chungnam National University, Daejeon 34134, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,10,10]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1002\/ame2.12223","article-title":"Advances in pig models of human diseases","volume":"5","author":"Hou","year":"2022","journal-title":"Anim. Model. Exp. Med."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"2308","DOI":"10.3389\/fimmu.2022.899657","article-title":"Progress in Xenotransplantation: Immunologic Barriers, Advances in Gene Editing, and Successful Tolerance Induction Strategies in Pig-To-Primate Transplantation","volume":"13","author":"Eisenson","year":"2022","journal-title":"Front. Immunol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"62","DOI":"10.1016\/j.ydbio.2005.05.001","article-title":"Development of porcine embryos in vivo and in vitro; evidence for embryo \u2018cross talk\u2019 in vitro","volume":"284","author":"Stokes","year":"2005","journal-title":"Dev. Biol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1089\/cell.2014.0003","article-title":"Improvement of Cloned Embryos Development by Co-Culturing with Parthenotes: A Possible Role of Exosomes\/Microvesicles for Embryos Paracrine Communication","volume":"16","author":"Saadeldin","year":"2014","journal-title":"Cell Reprogram."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1002\/mrd.23550","article-title":"Oviduct epithelial cells-derived extracellular vesicles improve preimplantation developmental competence of in vitro produced porcine parthenogenetic and cloned embryos","volume":"89","author":"Fang","year":"2021","journal-title":"Mol. Reprod. Dev."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1262\/jrd.47.303","article-title":"Development In Vivo and In Vitro to Blastocysts of Porcine Oocytes Matured and Fertilized In Vitro","volume":"47","author":"Kashiwazaki","year":"2001","journal-title":"J. Reprod. Dev."},{"key":"ref_7","first-page":"1","article-title":"Transcriptome dynamics in early in vivo developing and in vitro produced porcine embryos","volume":"22","author":"Schmidhauser","year":"2021","journal-title":"BMC. Genom."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"2","DOI":"10.12750\/JARB.37.1.2","article-title":"The theranostic roles of extracellular vesicles in pregnancy disorders","volume":"37","author":"Saadeldin","year":"2022","journal-title":"J. Anim. Reprod. Biotechnol."},{"key":"ref_9","first-page":"103","article-title":"Embryonic\u2013maternal cross-talk via exosomes: Potential implications","volume":"8","author":"Saadeldin","year":"2015","journal-title":"Stem. Cells Clon. Adv. Appl."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s12958-021-00801-5","article-title":"Trophoblast derived extracellular vesicles specifically alter the transcriptome of endometrial cells and may constitute a critical component of embryo-maternal communication","volume":"19","author":"Godakumara","year":"2021","journal-title":"Reprod. Biol. Endocrinol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1095\/biolreprod.115.134890","article-title":"Human Endometrial Exosomes Contain Hormone-Specific Cargo Modulating Trophoblast Adhesive Capacity: Insights into Endometrial-Embryo Interactions","volume":"94","author":"Greening","year":"2016","journal-title":"Biol. Reprod."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"760","DOI":"10.1016\/j.omtn.2021.09.009","article-title":"Placental trophoblast cell-derived exosomal microRNA-1290 promotes the interaction between endometrium and embryo by targeting LHX6","volume":"26","author":"Shi","year":"2021","journal-title":"Mol. Ther.-Nucleic. Acids"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1530\/REP-14-0538","article-title":"Exosomes, endogenous retroviruses and toll-like receptors: Pregnancy recognition in ewes","volume":"149","author":"Xu","year":"2015","journal-title":"Reproduction"},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Hirata, M., Wittayarat, M., Hirano, T., Nguyen, N.T., Le, Q.A., Namula, Z., Fahrudin, M., Tanihara, F., and Otoi, T. (2019). The Relationship between Embryonic Development and the Efficiency of Target Mutations in Porcine Endogenous Retroviruses (PERVs) Pol Genes in Porcine Embryos. Animals, 9.","DOI":"10.3390\/ani9090593"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1071\/RD06102","article-title":"Pregnancy recognition and conceptus implantation in domestic ruminants: Roles of progesterone, interferons and endogenous retroviruses","volume":"19","author":"Spencer","year":"2007","journal-title":"Reprod. Fertil. Dev."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1089\/cell.2018.0020","article-title":"A Rho-Associated Coiled-Coil Containing Kinase Inhibitor, Y-27632, Improves Viability of Dissociated Single Cells, Efficiency of Colony Formation, and Cryopreservation in Porcine Pluripotent Stem Cells","volume":"21","author":"Baek","year":"2019","journal-title":"Cell Reprogram."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"577","DOI":"10.1016\/j.jbiosc.2012.07.013","article-title":"Application of Rho-associated protein kinase (ROCK) inhibitor to human pluripotent stem cells","volume":"114","author":"Kurosawa","year":"2012","journal-title":"J. Biosci. Bioeng."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"616762","DOI":"10.3389\/fcell.2020.616762","article-title":"Rocking the Boat: The Decisive Roles of Rho Kinases During Oocyte, Blastocyst, and Stem Cell Development","volume":"8","author":"Saadeldin","year":"2021","journal-title":"Front. Cell Dev. Biol."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Motomura, K., Okada, N., Morita, H., Hara, M., Tamari, M., Orimo, K., Matsuda, G., Imadome, K.-I., Matsuda, A., and Nagamatsu, T. (2017). A Rho-associated coiled-coil containing kinases (ROCK) inhibitor, Y-27632, enhances adhesion, viability and differentiation of human term placenta-derived trophoblasts in vitro. PLoS ONE, 12.","DOI":"10.1371\/journal.pone.0177994"},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Hou, D., Su, M., Li, X., Li, Z., Yun, T., Zhao, Y., Zhang, M., Zhao, L., Li, R., and Yu, H. (2015). The Efficient Derivation of Trophoblast Cells from Porcine In Vitro Fertilized and Parthenogenetic Blastocysts and Culture with ROCK Inhibitor Y-27632. PLoS ONE, 10.","DOI":"10.1371\/journal.pone.0142442"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"90","DOI":"10.1016\/j.theriogenology.2022.02.019","article-title":"MiRNA-155 inhibition enhances porcine embryo preimplantation developmental competence by upregulating ZEB2 and downregulating ATF4","volume":"183","author":"Tanga","year":"2022","journal-title":"Theriogenology"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"286","DOI":"10.1016\/j.placenta.2014.02.003","article-title":"Post-maturation zona perforation improves porcine parthenogenetic trophoblast culture","volume":"35","author":"Saadeldin","year":"2014","journal-title":"Placenta"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"3005","DOI":"10.2527\/1991.6973005x","article-title":"Pig endometrial cells in primary culture: Morphology, secretion of prostaglandins and proteins, and effects of pregnancy","volume":"69","author":"Zhang","year":"1991","journal-title":"J. Anim. Sci."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Ng, Y.H., Rome, S., Jalabert, A., Forterre, A., Singh, H., Hincks, C.L., and Salamonsen, L.A. (2013). Endometrial Exosomes\/Microvesicles in the Uterine Microenvironment: A New Paradigm for Embryo-Endometrial Cross Talk at Implantation. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0058502"},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Abumaghaid, M.M., Abdelazim, A.M., Belali, T.M., Alhujaily, M., and Saadeldin, I.M. (2022). Shuttle transfer of mRNA transcripts via extracellular vesicles from male reproductive tract cells to the cumulus\u2013oocyte complex in rabbits (Oryctolagus cuniculus). Front. Veter. Sci.","DOI":"10.3389\/fvets.2022.816080"},{"key":"ref_26","first-page":"e50974","article-title":"An Innovative Method for Exosome Quantification and Size Measurement","volume":"95","author":"Mehdiani","year":"2015","journal-title":"J. Vis. Exp."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"109165","DOI":"10.1016\/j.vetmic.2021.109165","article-title":"Streptococcus equi-derived Extracellular Vesicles as a Vaccine Candidate against Streptococcus equi Infection","volume":"259","author":"Lee","year":"2021","journal-title":"Veter. Microbiol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"362","DOI":"10.1111\/dgd.12215","article-title":"Blastomeres aggregation as an efficient alternative for trophoblast culture from porcine parthenogenetic embryos","volume":"57","author":"Saadeldin","year":"2015","journal-title":"Dev. Growth Differ."},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Tukur, H.A., Aljumaah, R.S., Swelum, A.A.-A., Alowaimer, A.N., Abdelrahman, M., and Saadeldin, I.M. (2020). Effects of Short-Term Inhibition of Rho Kinase on Dromedary Camel Oocyte In Vitro Maturation. Animals, 10.","DOI":"10.3390\/ani10050750"},{"key":"ref_30","first-page":"1","article-title":"Follicular extracellular vesicles enhance meiotic resumption of domestic cat vitrified oocytes","volume":"10","author":"Ferraz","year":"2020","journal-title":"Sci. Rep."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1582237","DOI":"10.1080\/20013078.2019.1582237","article-title":"Pitfalls associated with lipophilic fluorophore staining of extracellular vesicles for uptake studies","volume":"8","author":"Simonsen","year":"2019","journal-title":"J. Extracell. Vesicles"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1388731","DOI":"10.1080\/20013078.2017.1388731","article-title":"Confounding factors in vesicle uptake studies using fluorescent lipophilic membrane dyes","volume":"6","author":"Takov","year":"2017","journal-title":"J. Extracell. Vesicles"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"656","DOI":"10.1071\/RD11118","article-title":"Paradoxical effects of kisspeptin: It enhances oocyte in vitro maturation but has an adverse impact on hatched blastocysts during in vitro culture","volume":"24","author":"Saadeldin","year":"2012","journal-title":"Reprod. Fertil. Dev."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s12943-019-0977-3","article-title":"MiR155 sensitized B-lymphoma cells to anti-PD-L1 antibody via PD-1\/PD-L1-mediated lymphoma cell interaction with CD8+T cells","volume":"18","author":"Zheng","year":"2019","journal-title":"Mol. Cancer"},{"key":"ref_35","first-page":"575","article-title":"Effects of microRNA-155 on the growth of human lung cancer cell line 95D in vitro","volume":"14","author":"Qin","year":"2011","journal-title":"Zhongguo Fei Ai Za Zhi"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1016\/j.jare.2019.11.009","article-title":"Thermotolerance and plasticity of camel somatic cells exposed to acute and chronic heat stress","volume":"22","author":"Saadeldin","year":"2020","journal-title":"J. Adv. Res."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1186\/1746-4811-3-12","article-title":"Protocol: A highly sensitive RT-PCR method for detection and quantification of microRNAs","volume":"3","author":"Wu","year":"2007","journal-title":"Plant Methods"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1283","DOI":"10.1095\/biolreprod58.5.1283","article-title":"Expression of G1 Cyclins and Cyclin-Dependent Kinase-2 Activity during Terminal Differentiation of Cultured Human Trophoblast1","volume":"58","author":"McKenzie","year":"1998","journal-title":"Biol. Reprod."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1080\/10799893.2020.1787444","article-title":"LASP2 inhibits trophoblast cell migration and invasion in preeclampsia through inactivation of the Wnt\/\u03b2-catenin signaling pathway","volume":"41","author":"Chen","year":"2020","journal-title":"J. Recept. Signal Transduct."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"206","DOI":"10.1177\/0300060518799265","article-title":"Expression of \u03b2-catenin in human trophoblast and its role in placenta accreta and placenta previa","volume":"47","author":"Han","year":"2018","journal-title":"J. Int. Med. Res."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"1577","DOI":"10.1093\/biolre\/ioab186","article-title":"Conceptus interferon gamma is essential for establishment of pregnancy in the pig","volume":"105","author":"Johns","year":"2021","journal-title":"Biol. Reprod."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"418","DOI":"10.4161\/cam.26375","article-title":"Protein tyrosine phosphatase PTP1B in cell adhesion and migration","volume":"7","author":"Arregui","year":"2013","journal-title":"Cell Adhes. Migr."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"377","DOI":"10.1016\/j.omtn.2018.12.011","article-title":"The Attenuation of Trophoblast Invasion Caused by the Downregulation of EZH2 Is Involved in the Pathogenesis of Human Recurrent Miscarriage","volume":"14","author":"Lv","year":"2018","journal-title":"Mol. Ther.-Nucleic. Acids"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"765","DOI":"10.1530\/REP-17-0254","article-title":"E-cadherin and ZEB2 modulate trophoblast cell differentiation during placental development in pigs","volume":"154","author":"Hong","year":"2017","journal-title":"Reproduction"},{"key":"ref_45","first-page":"61","article-title":"ZEB2, a master regulator of the epithelial\u2013mesenchymal transition, mediates trophoblast differentiation","volume":"25","author":"Kuo","year":"2018","journal-title":"Mol. Hum. Reprod."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"5713","DOI":"10.1021\/acsomega.9b03723","article-title":"Analysis of the Extracellular Proteome of Colistin-Resistant Korean Acinetobacter baumannii Strains","volume":"5","author":"Lee","year":"2020","journal-title":"ACS Omega"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"W216","DOI":"10.1093\/nar\/gkac194","article-title":"DAVID: A web server for functional enrichment analysis and functional annotation of gene lists (2021 update)","volume":"50","author":"Sherman","year":"2022","journal-title":"Nucleic Acids Res."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"e13360","DOI":"10.1111\/aji.13360","article-title":"Extracellular vesicles in embryo implantation and disorders of the endometrium","volume":"85","author":"Mishra","year":"2020","journal-title":"Am. J. Reprod. Immunol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"e1800423","DOI":"10.1002\/pmic.201800423","article-title":"Human Endometrial Extracellular Vesicles Functionally Prepare Human Trophectoderm Model for Implantation: Understanding Bidirectional Maternal-Embryo Communication","volume":"19","author":"Evans","year":"2019","journal-title":"Proteomics"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"510","DOI":"10.1093\/molehr\/gaaa034","article-title":"Exosomes and soluble secretome from hormone-treated endometrial epithelial cells direct embryo implantation","volume":"26","author":"Gurung","year":"2020","journal-title":"Mol. Hum. Reprod."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"1020","DOI":"10.1093\/biolre\/ioaa019","article-title":"Characterization and regulation of extracellular vesicles in the lumen of the ovine uterusdagger","volume":"102","author":"Burns","year":"2020","journal-title":"Biol. Reprod."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"238","DOI":"10.1097\/PGP.0000000000000233","article-title":"Stromal Clues in Endometrial Carcinoma: Loss of Expression of \u03b2-Catenin, Epithelial-Mesenchymal Transition Regulators, and Estrogen-Progesterone Receptor","volume":"35","author":"Senol","year":"2016","journal-title":"Int. J. Gynecol. Pathol."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"2543","DOI":"10.1111\/jcmm.17226","article-title":"Effect of MiR-100-5p on proliferation and apoptosis of goat endometrial stromal cell in vitro and embryo implantation in vivo","volume":"26","author":"Ma","year":"2022","journal-title":"J. Cell Mol. Med."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1016\/j.omtn.2020.10.043","article-title":"Endometrial cell-derived small extracellular vesicle miR-100-5p promotes functions of trophoblast during embryo implantation","volume":"23","author":"Tan","year":"2020","journal-title":"Mol. Ther.-Nucleic. Acids"},{"key":"ref_55","doi-asserted-by":"crossref","unstructured":"Su, L., Liu, R., Cheng, W., Zhu, M., Li, X., Zhao, S., and Yu, M. (2014). Expression Patterns of MicroRNAs in Porcine Endometrium and Their Potential Roles in Embryo Implantation and Placentation. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0087867"},{"key":"ref_56","first-page":"9837","article-title":"MicroRNA-132 stimulates the growth and invasiveness of trophoblasts by targeting DAPK-1","volume":"24","author":"Wang","year":"2020","journal-title":"Eur. Rev. Med. Pharm. Sci."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"995","DOI":"10.1016\/j.theriogenology.2010.11.006","article-title":"Effect of different culture media on the temporal gene expression in the bovine developing embryos","volume":"75","author":"Saadeldin","year":"2011","journal-title":"Theriogenology"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"513","DOI":"10.1016\/j.theriogenology.2010.09.019","article-title":"Analysis of co-expression of OCT4, NANOG and SOX2 in pluripotent cells of the porcine embryo, in vivo and in vitro","volume":"75","author":"Lopes","year":"2011","journal-title":"Theriogenology"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"779","DOI":"10.1095\/biolreprod.111.092809","article-title":"Generation of Colonies of Induced Trophoblast Cells During Standard Reprogramming of Porcine Fibroblasts to Induced Pluripotent Stem Cells1","volume":"85","author":"Ezashi","year":"2011","journal-title":"Biol. Reprod."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"550","DOI":"10.3892\/mmr.2014.2214","article-title":"MicroRNA-155 inhibits migration of trophoblast cells and contributes to the pathogenesis of severe preeclampsia by regulating endothelial nitric oxide synthase","volume":"10","author":"Li","year":"2014","journal-title":"Mol. Med. Rep."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"824","DOI":"10.1016\/j.placenta.2012.07.012","article-title":"MicroRNA-155 inhibits proliferation and migration of human extravillous trophoblast derived HTR-8\/SVneo cells via down-regulating cyclin D1","volume":"33","author":"Dai","year":"2012","journal-title":"Placenta"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"439","DOI":"10.1177\/0960327120954252","article-title":"MicroRNA-155 is upregulated in the placentas of patients with preeclampsia and affects trophoblast apoptosis by targeting SHH\/GLi1\/BCL2","volume":"40","author":"Wang","year":"2020","journal-title":"Hum. Exp. Toxicol."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"15144","DOI":"10.1073\/pnas.0705917104","article-title":"MicroRNA regulation of cyclooxygenase-2 during embryo implantation","volume":"104","author":"Chakrabarty","year":"2007","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"1407","DOI":"10.1161\/HYPERTENSIONAHA.112.197301","article-title":"Essential Role of MicroRNA-155 in Regulating Endothelium-Dependent Vasorelaxation by Targeting Endothelial Nitric Oxide Synthase","volume":"60","author":"Sun","year":"2012","journal-title":"Hypertension"},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"833","DOI":"10.1152\/physiolgenomics.00079.2014","article-title":"TNF-\u03b1 alters the release and transfer of microparticle-encapsulated miRNAs from endothelial cells","volume":"46","author":"Alexy","year":"2014","journal-title":"Physiol. Genom."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"66740","DOI":"10.18632\/oncotarget.11458","article-title":"ROCK inhibitor reduces Myc-induced apoptosis and mediates immortalization of human keratinocytes","volume":"7","author":"Dakic","year":"2016","journal-title":"Oncotarget"},{"key":"ref_67","doi-asserted-by":"crossref","unstructured":"Kim, K., Min, S., Kim, D., Kim, H., and Roh, S. (2021). A Rho Kinase (ROCK) Inhibitor, Y-27632, Inhibits the Dissociation-Induced Cell Death of Salivary Gland Stem Cells. Molecules, 26.","DOI":"10.3390\/molecules26092658"},{"key":"ref_68","first-page":"3645","article-title":"Rho kinase proteins--pleiotropic modulators of cell survival and apoptosis","volume":"31","author":"Street","year":"2011","journal-title":"Anticancer Res."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1007\/s00005-007-0009-7","article-title":"Rho kinase in the regulation of cell death and survival","volume":"55","author":"Shi","year":"2007","journal-title":"Arch. Immunol. Ther. Exp."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"5808","DOI":"10.1210\/jc.2002-020376","article-title":"Small Guanosine TriphospataseRhoA and Rho-Associated Kinase as Regulators of Trophoblast Migration","volume":"87","author":"Shiokawa","year":"2002","journal-title":"J. Clin. Endocrinol. Metab."},{"key":"ref_71","first-page":"185","article-title":"Endogenous retrovirus-encoded Syncytin-2 contributes to exosome-mediated immunosuppression of T cells\u2020","volume":"102","author":"Lokossou","year":"2019","journal-title":"Biol. Reprod."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"14390","DOI":"10.1073\/pnas.0603836103","article-title":"Endogenous retroviruses regulate periimplantation placental growth and differentiation","volume":"103","author":"Dunlap","year":"2006","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_73","doi-asserted-by":"crossref","unstructured":"Fu, B., Ma, H., and Liu, D. (2019). Endogenous Retroviruses Function as Gene Expression Regulatory Elements During Mammalian Pre-implantation Embryo Development. Int. J. Mol. Sci., 20.","DOI":"10.3390\/ijms20030790"},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1159\/000084967","article-title":"Endogenous retroviruses and animal reproduction","volume":"110","author":"Prudhomme","year":"2005","journal-title":"Cytogenet. Genome. Res."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"23","DOI":"10.3389\/fchem.2017.00023","article-title":"Endogenous Retroviruses: With Us and against Us","volume":"5","author":"Meyer","year":"2017","journal-title":"Front. Chem."},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"1623","DOI":"10.1007\/s00705-016-2833-8","article-title":"Expression of the env gene from the avian endogenous retrovirus ALVE and regulation by miR-155","volume":"161","author":"Hu","year":"2016","journal-title":"Arch. Virol."}],"container-title":["Cells"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2073-4409\/11\/19\/3178\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T00:49:29Z","timestamp":1760143769000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2073-4409\/11\/19\/3178"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,10,10]]},"references-count":76,"journal-issue":{"issue":"19","published-online":{"date-parts":[[2022,10]]}},"alternative-id":["cells11193178"],"URL":"https:\/\/doi.org\/10.3390\/cells11193178","relation":{},"ISSN":["2073-4409"],"issn-type":[{"value":"2073-4409","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,10,10]]}}}