{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T20:25:33Z","timestamp":1791491133503,"version":"4.3.4"},"reference-count":61,"publisher":"MDPI AG","issue":"20","license":[{"start":{"date-parts":[[2022,10,21]],"date-time":"2022-10-21T00:00:00Z","timestamp":1666310400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.mdpi.com"],"crossmark-restriction":true},"short-container-title":["Cells"],"abstract":"<jats:p>A reduced nephron number at birth, due to critical gestational conditions, including maternal malnutrition, is associated with the risk of developing hypertension and chronic kidney disease in adulthood. No interventions are currently available to augment nephron number. We have recently shown that sirtuin 3 (SIRT3) has an important role in dictating proper nephron endowment. The present study explored whether SIRT3 stimulation, by means of supplementation with nicotinamide riboside (NR), a precursor of the SIRT3 co-substrate nicotinamide adenine dinucleotide (NAD+), was able to improve nephron number in a murine model of a low protein (LP) diet. Our findings show that reduced nephron number in newborn mice (day 1) born to mothers fed a LP diet was associated with impaired renal SIRT3 expression, which was restored through supplementation with NR. Glomerular podocyte density, as well as the rarefaction of renal capillaries, also improved through NR administration. In mechanistic terms, the restoration of SIRT3 expression through NR was mediated by the induction of proliferator-activated receptor \u03b3 (PPAR\u03b3) coactivator-1\u03b1 (PGC-1\u03b1). Moreover, NR restored SIRT3 activity, as shown by the reduction of the acetylation of optic atrophy 1 (OPA1) and superoxide dismutase 2 (SOD2), which resulted in improved mitochondrial morphology and protection against oxidative damage in mice born to mothers fed the LP diet. Our results provide evidence that it is feasible to prevent nephron mass shortage at birth through SIRT3 boosting during nephrogenesis, thus providing a therapeutic option to possibly limit the long-term sequelae of reduced nephron number in adulthood.<\/jats:p>","DOI":"10.3390\/cells11203316","type":"journal-article","created":{"date-parts":[[2022,10,23]],"date-time":"2022-10-23T20:43:50Z","timestamp":1666557830000},"page":"3316","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Low Nephron Number Induced by Maternal Protein Restriction Is Prevented by Nicotinamide Riboside Supplementation Depending on Sirtuin 3 Activation"],"prefix":"10.3390","volume":"11","author":[{"given":"Anna","family":"Pezzotta","sequence":"first","affiliation":[{"name":"Istituto di Ricerche Farmacologiche Mario Negri IRCCS, Centro Anna Maria Astori, Science and Technology Park Kilometro Rosso, 24126 Bergamo, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3470-1721","authenticated-orcid":false,"given":"Luca","family":"Perico","sequence":"additional","affiliation":[{"name":"Istituto di Ricerche Farmacologiche Mario Negri IRCCS, Centro Anna Maria Astori, Science and Technology Park Kilometro Rosso, 24126 Bergamo, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marina","family":"Morigi","sequence":"additional","affiliation":[{"name":"Istituto di Ricerche Farmacologiche Mario Negri IRCCS, Centro Anna Maria Astori, Science and Technology Park Kilometro Rosso, 24126 Bergamo, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniela","family":"Corna","sequence":"additional","affiliation":[{"name":"Istituto di Ricerche Farmacologiche Mario Negri IRCCS, Centro Anna Maria Astori, Science and Technology Park Kilometro Rosso, 24126 Bergamo, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1395-3172","authenticated-orcid":false,"given":"Monica","family":"Locatelli","sequence":"additional","affiliation":[{"name":"Istituto di Ricerche Farmacologiche Mario Negri IRCCS, Centro Anna Maria Astori, Science and Technology Park Kilometro Rosso, 24126 Bergamo, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlamaria","family":"Zoja","sequence":"additional","affiliation":[{"name":"Istituto di Ricerche Farmacologiche Mario Negri IRCCS, Centro Anna Maria Astori, Science and Technology Park Kilometro Rosso, 24126 Bergamo, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ariela","family":"Benigni","sequence":"additional","affiliation":[{"name":"Istituto di Ricerche Farmacologiche Mario Negri IRCCS, Centro Anna Maria Astori, Science and Technology Park Kilometro Rosso, 24126 Bergamo, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6194-3446","authenticated-orcid":false,"given":"Giuseppe","family":"Remuzzi","sequence":"additional","affiliation":[{"name":"Istituto di Ricerche Farmacologiche Mario Negri IRCCS, Centro Anna Maria Astori, Science and Technology Park Kilometro Rosso, 24126 Bergamo, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5005-2536","authenticated-orcid":false,"given":"Barbara","family":"Imberti","sequence":"additional","affiliation":[{"name":"Istituto di Ricerche Farmacologiche Mario Negri IRCCS, Centro Anna Maria Astori, Science and Technology Park Kilometro Rosso, 24126 Bergamo, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,10,21]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1842","DOI":"10.1016\/S0140-6736(18)30312-X","article-title":"Origins of Lifetime Health around the Time of Conception: Causes and Consequences","volume":"391","author":"Fleming","year":"2018","journal-title":"Lancet"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"770","DOI":"10.1046\/j.1523-1755.2000.00225.x","article-title":"Relationship between Weight at Birth and the Number and Size of Renal Glomeruli in Humans: A Histomorphometric Study","volume":"58","author":"Reyes","year":"2000","journal-title":"Kidney Int."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"296","DOI":"10.1111\/j.1471-0528.1992.tb13726.x","article-title":"The Effect of Intrauterine Growth Retardation on the Development of Renal Nephrons","volume":"99","author":"Hinchliffe","year":"1992","journal-title":"Br. J. Obstet. Gynaecol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1038\/nrneph.2014.251","article-title":"Birth Weight, Malnutrition and Kidney-Associated Outcomes\u2014A Global Concern","volume":"11","author":"Luyckx","year":"2015","journal-title":"Nat. Rev. Nephrol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"688","DOI":"10.1038\/s41581-018-0054-y","article-title":"Maternal and Environmental Risk Factors for Neonatal AKI and Its Long-Term Consequences","volume":"14","author":"Perico","year":"2018","journal-title":"Nat. Rev. Nephrol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"335","DOI":"10.1093\/ajh\/1.4.335","article-title":"Glomeruli and Blood Pressure. Less of One, More the Other?","volume":"1","author":"Brenner","year":"1988","journal-title":"Am. J. Hypertens."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1056\/NEJMoa020549","article-title":"Nephron Number in Patients with Primary Hypertension","volume":"348","author":"Keller","year":"2003","journal-title":"N. Engl. J. Med."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1053\/j.ajkd.2008.12.042","article-title":"Is Low Birth Weight an Antecedent of CKD in Later Life? A Systematic Review of Observational Studies","volume":"54","author":"White","year":"2009","journal-title":"Am. J. Kidney Dis."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1233","DOI":"10.1001\/jama.2011.1331","article-title":"Gestational Age at Birth and Mortality in Young Adulthood","volume":"306","author":"Crump","year":"2011","journal-title":"JAMA"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"553","DOI":"10.2215\/CJN.02290217","article-title":"Can Renal Biopsy Be Used to Estimate Total Nephron Number?","volume":"12","author":"Charlton","year":"2017","journal-title":"Clin. J. Am. Soc. Nephrol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1016\/S0140-6736(13)60311-6","article-title":"Effect of Fetal and Child Health on Kidney Development and Long-Term Risk of Hypertension and Kidney Disease","volume":"382","author":"Luyckx","year":"2013","journal-title":"Lancet"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"183","DOI":"10.3389\/fcell.2020.00183","article-title":"Cellular and Molecular Mechanisms of Kidney Development: From the Embryo to the Kidney Organoid","volume":"8","author":"Aghdami","year":"2020","journal-title":"Front. Cell. Dev. Biol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"23580","DOI":"10.1038\/s41598-021-03039-8","article-title":"Post-Translational Modifications by SIRT3 de-2-Hydroxyisobutyrylase Activity Regulate Glycolysis and Enable Nephrogenesis","volume":"11","author":"Perico","year":"2021","journal-title":"Sci. Rep."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"647","DOI":"10.1083\/jcb.200205057","article-title":"The Human Silent Information Regulator (Sir)2 Homologue HSIRT3 Is a Mitochondrial Nicotinamide Adenine Dinucleotide-Dependent Deacetylase","volume":"158","author":"Schwer","year":"2002","journal-title":"J. Cell Biol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"13653","DOI":"10.1073\/pnas.222538099","article-title":"SIRT3, a Human SIR2 Homologue, Is an NAD-Dependent Deacetylase Localized to Mitochondria","volume":"99","author":"Onyango","year":"2002","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"8807","DOI":"10.1128\/MCB.01636-07","article-title":"Mammalian Sir2 Homolog SIRT3 Regulates Global Mitochondrial Lysine Acetylation","volume":"27","author":"Lombard","year":"2007","journal-title":"Mol. Cell. Biol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"524","DOI":"10.1172\/JCI36703","article-title":"Disruption of the Ang II Type 1 Receptor Promotes Longevity in Mice","volume":"119","author":"Benigni","year":"2009","journal-title":"J. Clin. Investig."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"715","DOI":"10.1172\/JCI77632","article-title":"Sirtuin 3-Dependent Mitochondrial Dynamic Improvements Protect against Acute Kidney Injury","volume":"125","author":"Morigi","year":"2015","journal-title":"J. Clin. Investig."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"R1768","DOI":"10.1152\/ajpregu.00442.2006","article-title":"Effects of Dietary Protein Restriction on Nephron Number in the Mouse","volume":"292","author":"Hoppe","year":"2007","journal-title":"Am. J. Physiol. Regul. Integr. Comp. Physiol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"S47","DOI":"10.1097\/01.hjh.0000358838.71675.5e","article-title":"Hypertension and Kidney Alterations in Rat Offspring from Low Protein Pregnancies","volume":"27","author":"Carvalho","year":"2009","journal-title":"J. Hypertens. Suppl."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"F322","DOI":"10.1152\/ajprenal.00029.2021","article-title":"Podocyte Endowment and the Impact of Adult Body Size on Kidney Health","volume":"321","author":"Haruhara","year":"2021","journal-title":"Am. J. Physiol. Renal. Physiol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"732","DOI":"10.1016\/j.bbrc.2019.04.057","article-title":"Intrauterine Low-Protein Diet Disturbs Metanephric Gene Expression and Induces Urinary Tract Developmental Abnormalities in Mice","volume":"513","author":"Yu","year":"2019","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"F43","DOI":"10.1152\/ajprenal.00405.2019","article-title":"Intrauterine Low-Protein Diet Aggravates Developmental Abnormalities of the Urinary System via the Akt\/Creb3 Pathway in Robo2 Mutant Mice","volume":"318","author":"Yu","year":"2020","journal-title":"Am. J. Physiol. Renal. Physiol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"795","DOI":"10.1038\/35001622","article-title":"Transcriptional Silencing and Longevity Protein Sir2 Is an NAD-Dependent Histone Deacetylase","volume":"403","author":"Imai","year":"2000","journal-title":"Nature"},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Kong, X., Wang, R., Xue, Y., Liu, X., Zhang, H., Chen, Y., Fang, F., and Chang, Y. (2010). Sirtuin 3, a New Target of PGC-1alpha, Plays an Important Role in the Suppression of ROS and Mitochondrial Biogenesis. PLoS ONE, 5.","DOI":"10.1371\/journal.pone.0011707"},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Percie du Sert, N., Ahluwalia, A., Alam, S., Avey, M.T., Baker, M., Browne, W.J., Clark, A., Cuthill, I.C., Dirnagl, U., and Emerson, M. (2020). Reporting Animal Research: Explanation and Elaboration for the ARRIVE Guidelines 2.0. PLOS Biol., 18.","DOI":"10.1371\/journal.pbio.3000411"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"983","DOI":"10.1038\/s41467-017-00937-2","article-title":"Human Mesenchymal Stromal Cells Transplanted into Mice Stimulate Renal Tubular Cells and Enhance Mitochondrial Function","volume":"8","author":"Perico","year":"2017","journal-title":"Nat. Commun."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"393","DOI":"10.1111\/j.1365-2818.1989.tb02898.x","article-title":"Measuring through the Microscope: Development and Evolution of Stereological Methods","volume":"155","author":"Weibel","year":"1989","journal-title":"J. Microsc."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"8418","DOI":"10.1038\/s41598-020-65423-0","article-title":"Manipulating Sirtuin 3 Pathway Ameliorates Renal Damage in Experimental Diabetes","volume":"10","author":"Locatelli","year":"2020","journal-title":"Sci. Rep."},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"de Barros Sene, L., Scarano, W.R., Zapparoli, A., Gontijo, J.A.R., and Boer, P.A. (2021). Impact of Gestational Low-Protein Intake on Embryonic Kidney MicroRNA Expression and in Nephron Progenitor Cells of the Male Fetus. PLoS ONE, 16.","DOI":"10.1101\/2020.02.18.954800"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"807","DOI":"10.1128\/MCB.01483-13","article-title":"SIRT3 Deacetylates and Activates OPA1 to Regulate Mitochondrial Dynamics during Stress","volume":"34","author":"Samant","year":"2014","journal-title":"Mol. Cell. Biol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1255","DOI":"10.1089\/ars.2018.7703","article-title":"Sirt3 Deficiency Shortens Life Span and Impairs Cardiac Mitochondrial Function Rescued by Opa1 Gene Transfer","volume":"31","author":"Benigni","year":"2019","journal-title":"Antioxid. Redox Signal."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"838","DOI":"10.1016\/j.cmet.2012.04.022","article-title":"The NAD+ Precursor Nicotinamide Riboside Enhances Oxidative Metabolism and Protects against High-Fat Diet-Induced Obesity","volume":"15","author":"Houtkooper","year":"2012","journal-title":"Cell Metab."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"534","DOI":"10.1038\/embor.2011.65","article-title":"Tumour Suppressor SIRT3 Deacetylates and Activates Manganese Superoxide Dismutase to Scavenge ROS","volume":"12","author":"Chen","year":"2011","journal-title":"EMBO Rep."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"R1516","DOI":"10.1152\/ajpregu.00280.2009","article-title":"Maternal Low-Protein Diet Alters Pancreatic Islet Mitochondrial Function in a Sex-Specific Manner in the Adult Rat","volume":"297","author":"Theys","year":"2009","journal-title":"Am. J. Physiol. Regul. Integr. Comp. Physiol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1622","DOI":"10.1016\/j.numecd.2021.01.006","article-title":"Moderate Offspring Exercise Offsets the Harmful Effects of Maternal Protein Deprivation on Mitochondrial Function and Oxidative Balance by Modulating Sirtuins","volume":"31","author":"Bernardo","year":"2021","journal-title":"Nutr. Metab. Cardiovasc. Dis."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"203","DOI":"10.3389\/fnins.2019.00203","article-title":"Maternal Protein Restriction in Two Successive Generations Impairs Mitochondrial Electron Coupling in the Progeny\u2019s Brainstem of Wistar Rats from Both Sexes","volume":"13","author":"Santana","year":"2019","journal-title":"Front. Neurosci."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"880","DOI":"10.1139\/apnm-2013-0452","article-title":"The Effect of Maternal Low-Protein Diet on the Heart of Adult Offspring: Role of Mitochondria and Oxidative Stress","volume":"39","author":"Nascimento","year":"2014","journal-title":"Appl. Physiol. Nutr. Metab."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"S38","DOI":"10.1186\/1423-0127-17-S1-S38","article-title":"A Maternal Low Protein Diet Has Pronounced Effects on Mitochondrial Gene Expression in Offspring Liver and Skeletal Muscle; Protective Effect of Taurine","volume":"17","author":"Mortensen","year":"2010","journal-title":"J. Biomed. Sci."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"414D","DOI":"10.2471\/BLT.17.206441","article-title":"The Global Burden of Kidney Disease and the Sustainable Development Goals","volume":"96","author":"Luyckx","year":"2018","journal-title":"Bull. World Health Organ."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"424","DOI":"10.1016\/S0140-6736(17)30576-7","article-title":"A Developmental Approach to the Prevention of Hypertension and Kidney Disease: A Report from the Low Birth Weight and Nephron Number Working Group","volume":"390","author":"Luyckx","year":"2017","journal-title":"Lancet"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1016\/j.kisu.2021.11.003","article-title":"Epidemiology of Chronic Kidney Disease: An Update 2022","volume":"12","author":"Kovesdy","year":"2022","journal-title":"Kidney Int. Suppl."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"9772","DOI":"10.1038\/s41598-019-46120-z","article-title":"Safety and Metabolism of Long-Term Administration of NIAGEN (Nicotinamide Riboside Chloride) in a Randomized, Double-Blind, Placebo-Controlled Clinical Trial of Healthy Overweight Adults","volume":"9","author":"Conze","year":"2019","journal-title":"Sci. Rep."},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Mehmel, M., Jovanovi\u0107, N., and Spitz, U. (2020). Nicotinamide Riboside-The Current State of Research and Therapeutic Uses. Nutrients, 12.","DOI":"10.3390\/nu12061616"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"1581","DOI":"10.1016\/j.neurobiolaging.2012.12.005","article-title":"Nicotinamide Riboside Restores Cognition through an Upregulation of Proliferator-Activated Receptor-\u03b3 Coactivator 1\u03b1 Regulated \u03b2-Secretase 1 Degradation and Mitochondrial Gene Expression in Alzheimer\u2019s Mouse Models","volume":"34","author":"Gong","year":"2013","journal-title":"Neurobiol. Aging"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"361","DOI":"10.1016\/j.cmet.2005.05.004","article-title":"Metabolic Control through the PGC-1 Family of Transcription Coactivators","volume":"1","author":"Lin","year":"2005","journal-title":"Cell Metab."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"615","DOI":"10.1172\/JCI27794","article-title":"PGC-1 Coactivators: Inducible Regulators of Energy Metabolism in Health and Disease","volume":"116","author":"Finck","year":"2006","journal-title":"J. Clin. Investig."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"16958","DOI":"10.1074\/jbc.M110.202390","article-title":"Peroxisome Proliferator-Activated Receptor-\u03b3 Coactivator-1\u03b1 Controls Transcription of the Sirt3 Gene, an Essential Component of the Thermogenic Brown Adipocyte Phenotype*","volume":"286","author":"Giralt","year":"2011","journal-title":"J. Biol. Chem."},{"key":"ref_49","doi-asserted-by":"crossref","unstructured":"Koh, J.-H., and Kim, J.-Y. (2021). Role of PGC-1\u03b1 in the Mitochondrial NAD+ Pool in Metabolic Diseases. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms22094558"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"528","DOI":"10.1038\/nature17184","article-title":"PGC1\u03b1 Drives NAD Biosynthesis Linking Oxidative Metabolism to Renal Protection","volume":"531","author":"Tran","year":"2016","journal-title":"Nature"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"264","DOI":"10.1016\/j.kint.2019.03.012","article-title":"The INADequacy of Renal Cell Metabolism: Modulating NAD+ Biosynthetic Pathways to Forestall Kidney Diseases","volume":"96","author":"Perico","year":"2019","journal-title":"Kidney Int."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"1898","DOI":"10.1681\/ASN.2020091321","article-title":"Increasing MTORC1 Pathway Activity or Methionine Supplementation during Pregnancy Reverses the Negative Effect of Maternal Malnutrition on the Developing Kidney","volume":"32","author":"Makayes","year":"2021","journal-title":"J. Am. Soc. Nephrol."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"1059","DOI":"10.1016\/j.cmet.2014.11.003","article-title":"Activation of SIRT3 by the NAD+ Precursor Nicotinamide Riboside Protects from Noise-Induced Hearing Loss","volume":"20","author":"Brown","year":"2014","journal-title":"Cell Metab."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"662","DOI":"10.1016\/j.cmet.2010.11.015","article-title":"Calorie Restriction Reduces Oxidative Stress by SIRT3-Mediated SOD2 Activation","volume":"12","author":"Qiu","year":"2010","journal-title":"Cell Metab."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/s41419-020-03152-y","article-title":"OPA1 and MICOS Regulate Mitochondrial Crista Dynamics and Formation","volume":"11","author":"Hu","year":"2020","journal-title":"Cell Death Dis."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"101944","DOI":"10.1016\/j.redox.2021.101944","article-title":"Opa1 Relies on Cristae Preservation and ATP Synthase to Curtail Reactive Oxygen Species Accumulation in Mitochondria","volume":"41","author":"Corrado","year":"2021","journal-title":"Redox Biol."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1016\/j.mito.2019.11.006","article-title":"OPA1 Regulates Respiratory Supercomplexes Assembly: The Role of Mitochondrial Swelling","volume":"51","author":"Jang","year":"2020","journal-title":"Mitochondrion"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"1540","DOI":"10.1038\/s41590-020-0793-3","article-title":"Disturbed Mitochondrial Dynamics in CD8+ TILs Reinforce T Cell Exhaustion","volume":"21","author":"Yu","year":"2020","journal-title":"Nat. Immunol."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"108660","DOI":"10.1016\/j.celrep.2020.108660","article-title":"NAD+ Boosting Reduces Age-Associated Amyloidosis and Restores Mitochondrial Homeostasis in Muscle","volume":"34","author":"Romani","year":"2021","journal-title":"Cell Rep."},{"key":"ref_60","doi-asserted-by":"crossref","unstructured":"Cao, X., Wu, Y., Hong, H., and Tian, X.Y. (2022). Sirtuin 3 Dependent and Independent Effects of NAD+ to Suppress Vascular Inflammation and Improve Endothelial Function in Mice. 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