{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T00:08:29Z","timestamp":1770682109002,"version":"3.49.0"},"reference-count":62,"publisher":"MDPI AG","issue":"22","license":[{"start":{"date-parts":[[2022,11,17]],"date-time":"2022-11-17T00:00:00Z","timestamp":1668643200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"NIH","award":["HL114734"],"award-info":[{"award-number":["HL114734"]}]},{"name":"NIH","award":["HL132155"],"award-info":[{"award-number":["HL132155"]}]},{"name":"NIH","award":["16BGIA27790081"],"award-info":[{"award-number":["16BGIA27790081"]}]},{"name":"NIH","award":["18CDA34110132"],"award-info":[{"award-number":["18CDA34110132"]}]},{"name":"AHA","award":["HL114734"],"award-info":[{"award-number":["HL114734"]}]},{"name":"AHA","award":["HL132155"],"award-info":[{"award-number":["HL132155"]}]},{"name":"AHA","award":["16BGIA27790081"],"award-info":[{"award-number":["16BGIA27790081"]}]},{"name":"AHA","award":["18CDA34110132"],"award-info":[{"award-number":["18CDA34110132"]}]},{"name":"AHA CDA","award":["HL114734"],"award-info":[{"award-number":["HL114734"]}]},{"name":"AHA CDA","award":["HL132155"],"award-info":[{"award-number":["HL132155"]}]},{"name":"AHA CDA","award":["16BGIA27790081"],"award-info":[{"award-number":["16BGIA27790081"]}]},{"name":"AHA CDA","award":["18CDA34110132"],"award-info":[{"award-number":["18CDA34110132"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells"],"abstract":"<jats:p>Accumulating evidence indicates that the APOA1 binding protein (AIBP)\u2014a secreted protein\u2014plays a profound role in lipid metabolism. Interestingly, AIBP also functions as an NAD(P)H-hydrate epimerase to catalyze the interconversion of NAD(P)H hydrate [NAD(P)HX] epimers and is renamed as NAXE. Thus, we call it NAXE hereafter. We investigated its role in NAD(P)H-involved metabolism in murine cardiomyocytes, focusing on the metabolism of hexose, lipids, and amino acids as well as mitochondrial redox function. Unbiased metabolite profiling of cardiac tissue shows that NAXE knockout markedly upregulates the ketone body 3-hydroxybutyric acid (3-HB) and increases or trends increasing lipid-associated metabolites cholesterol, \u03b1-linolenic acid and deoxycholic acid. Paralleling greater ketone levels, ChemRICH analysis of the NAXE-regulated metabolites shows reduced abundance of hexose despite similar glucose levels in control and NAXE-deficient blood. NAXE knockout reduces cardiac lactic acid but has no effect on the content of other NAD(P)H-regulated metabolites, including those associated with glucose metabolism, the pentose phosphate pathway, or Krebs cycle flux. Although NAXE is present in mitochondria, it has no apparent effect on mitochondrial oxidative phosphorylation. Instead, we detected more metabolites that can potentially improve cardiac function (3-HB, adenosine, and \u03b1-linolenic acid) in the Naxe\u2212\/\u2212 heart; these mice also perform better in aerobic exercise. Our data reveal a new role of NAXE in cardiac ketone and lipid metabolism.<\/jats:p>","DOI":"10.3390\/cells11223643","type":"journal-article","created":{"date-parts":[[2022,11,17]],"date-time":"2022-11-17T03:26:04Z","timestamp":1668655564000},"page":"3643","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["AIBP Regulates Metabolism of Ketone and Lipids but Not Mitochondrial Respiration"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9747-6288","authenticated-orcid":false,"given":"Jun-dae","family":"Kim","sequence":"first","affiliation":[{"name":"Center for Cardiovascular Regeneration, Department of Cardiovascular Sciences, Houston Methodist Research Institute, 6550 Fannin St., Houston, TX 77030, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7709-0265","authenticated-orcid":false,"given":"Teng","family":"Zhou","sequence":"additional","affiliation":[{"name":"Center for Cardiovascular Regeneration, Department of Cardiovascular Sciences, Houston Methodist Research Institute, 6550 Fannin St., Houston, TX 77030, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1491-8528","authenticated-orcid":false,"given":"Aijun","family":"Zhang","sequence":"additional","affiliation":[{"name":"Center for Bioenergetics, Houston Methodist Research Institute, 6550 Fannin St., Houston, TX 77030, USA"},{"name":"Department of Medicine, Houston Methodist, Weill Cornell Medicine Affiliate, 6550 Fannin St., Houston, TX 77030, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shumin","family":"Li","sequence":"additional","affiliation":[{"name":"Center for Bioenergetics, Houston Methodist Research Institute, 6550 Fannin St., Houston, TX 77030, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anisha A.","family":"Gupte","sequence":"additional","affiliation":[{"name":"Center for Bioenergetics, Houston Methodist Research Institute, 6550 Fannin St., Houston, TX 77030, USA"},{"name":"Department of Medicine, Houston Methodist, Weill Cornell Medicine Affiliate, 6550 Fannin St., Houston, TX 77030, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2481-2746","authenticated-orcid":false,"given":"Dale J.","family":"Hamilton","sequence":"additional","affiliation":[{"name":"Center for Bioenergetics, Houston Methodist Research Institute, 6550 Fannin St., Houston, TX 77030, USA"},{"name":"Department of Medicine, Houston Methodist, Weill Cornell Medicine Affiliate, 6550 Fannin St., Houston, TX 77030, USA"},{"name":"Weill Cornell Medical College, Cornell University, 407 E 61st St., New York, NY 10065, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1653-5221","authenticated-orcid":false,"given":"Longhou","family":"Fang","sequence":"additional","affiliation":[{"name":"Center for Cardiovascular Regeneration, Department of Cardiovascular Sciences, Houston Methodist Research Institute, 6550 Fannin St., Houston, TX 77030, USA"},{"name":"Department of Medicine, Houston Methodist, Weill Cornell Medicine Affiliate, 6550 Fannin St., Houston, TX 77030, USA"},{"name":"Weill Cornell Medical College, Cornell University, 407 E 61st St., New York, NY 10065, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,11,17]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2108","DOI":"10.1210\/en.2007-0582","article-title":"Biochemical and structural characterization of apolipoprotein A-I binding protein, a novel phosphoprotein with a potential role in sperm 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