{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T05:07:32Z","timestamp":1784092052139,"version":"3.55.0"},"reference-count":28,"publisher":"MDPI AG","issue":"24","license":[{"start":{"date-parts":[[2022,12,10]],"date-time":"2022-12-10T00:00:00Z","timestamp":1670630400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"The Xinjiang Production and Construction Corps","award":["2020AB020"],"award-info":[{"award-number":["2020AB020"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells"],"abstract":"<jats:p>Researchers increasingly report the therapeutic effect of exosomes derived from rat bone marrow mesenchymal stem cells (Exos-rBMMSC) on liver disease, while the optimal dose of Exos-rBMMSC in liver cirrhotic treatment has not been reported. In this study, we aimed to explore the efficacy and dose of Exos-rBMMSC in a hepatic cirrhosis rat model. The therapeutic effects of a low dose, medium dose and high dose of Exos-rBMMSC were assessed by liver function tests and histopathology. After four-weeks of Exos-rBMMSC therapy, pyroptosis-related expression levels in the medium dose and the high dose Exos-rBMMSC groups were significantly decreased compared to those in the liver cirrhosis group (p &lt; 0.05). The hepatic function assay and histopathology results showed significant improvement in the medium dose and the high dose Exos-rBMMSCs groups. The localization of PKH67-labeled Exos-rBMMSC was verified microscopically, and these particles were coexpressed with the PCNA, NLRP3, GSDMD and Caspase-1. Our results demonstrated that Exos-rBMMSC accelerated hepatocyte proliferation and relieved liver fibrosis by restraining hepatocyte pyroptosis. More importantly, we confirmed that the high dose of Exos-rBMMSC may be the optimal dose for liver cirrhosis, which is conducive to the application of Exos-rBMMSC as a promising cell-free strategy.<\/jats:p>","DOI":"10.3390\/cells11244004","type":"journal-article","created":{"date-parts":[[2022,12,12]],"date-time":"2022-12-12T03:36:09Z","timestamp":1670816169000},"page":"4004","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Exosomes Derived from BMMSCs Mitigate the Hepatic Fibrosis via Anti-Pyroptosis Pathway in a Cirrhosis Model"],"prefix":"10.3390","volume":"11","author":[{"given":"Yichi","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Gastroenterology and Hepatology, The Second Affiliated Hospital, Harbin Medical University, Harbin 150086, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hanjing","family":"Zhangdi","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Hepatology, The Second Affiliated Hospital, Harbin Medical University, Harbin 150086, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinsheng","family":"Nie","sequence":"additional","affiliation":[{"name":"The Xinjiang Production and Construction Corps Tenth Division Beitun Hospital, Beitun 806099, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lijuan","family":"Wang","sequence":"additional","affiliation":[{"name":"The Xinjiang Production and Construction Corps Tenth Division Beitun Hospital, Beitun 806099, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhuzhi","family":"Wan","sequence":"additional","affiliation":[{"name":"The Xinjiang Production and Construction Corps Tenth Division Beitun Hospital, Beitun 806099, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hao","family":"Jin","sequence":"additional","affiliation":[{"name":"The Xinjiang Production and Construction Corps Tenth Division Beitun Hospital, Beitun 806099, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ronghui","family":"Pu","sequence":"additional","affiliation":[{"name":"The Xinjiang Production and Construction Corps Tenth Division Beitun Hospital, Beitun 806099, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meihui","family":"Liang","sequence":"additional","affiliation":[{"name":"The Xinjiang Production and Construction Corps Tenth Division Beitun Hospital, Beitun 806099, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuan","family":"Chang","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Hepatology, The Second Affiliated Hospital, Harbin Medical University, Harbin 150086, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Gao","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Hepatology, The Second Affiliated Hospital, Harbin Medical University, Harbin 150086, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hailong","family":"Zhang","sequence":"additional","affiliation":[{"name":"The Xinjiang Production and Construction Corps Tenth Division Beitun Hospital, Beitun 806099, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shizhu","family":"Jin","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Hepatology, The Second Affiliated Hospital, Harbin Medical University, Harbin 150086, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,12,10]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1016\/j.jhep.2018.09.014","article-title":"Burden of liver diseases in the world","volume":"70","author":"Asrani","year":"2019","journal-title":"J. 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