{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,13]],"date-time":"2026-09-13T03:17:54Z","timestamp":1789269474223,"version":"build-2803163510"},"reference-count":96,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2023,1,3]],"date-time":"2023-01-03T00:00:00Z","timestamp":1672704000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"the Russian Fund for Basic Research","award":["research project #20-015-00388"],"award-info":[{"award-number":["research project #20-015-00388"]}]},{"name":"the Russian Fund for Basic Research","award":["#075-00408-21-00"],"award-info":[{"award-number":["#075-00408-21-00"]}]},{"name":"the Russian Fund for Basic Research","award":["#22-15-00393"],"award-info":[{"award-number":["#22-15-00393"]}]},{"name":"the IEPhB Research Program","award":["research project #20-015-00388"],"award-info":[{"award-number":["research project #20-015-00388"]}]},{"name":"the IEPhB Research Program","award":["#075-00408-21-00"],"award-info":[{"award-number":["#075-00408-21-00"]}]},{"name":"the IEPhB Research Program","award":["#22-15-00393"],"award-info":[{"award-number":["#22-15-00393"]}]},{"name":"Russian Scientific Foundation","award":["research project #20-015-00388"],"award-info":[{"award-number":["research project #20-015-00388"]}]},{"name":"Russian Scientific Foundation","award":["#075-00408-21-00"],"award-info":[{"award-number":["#075-00408-21-00"]}]},{"name":"Russian Scientific Foundation","award":["#22-15-00393"],"award-info":[{"award-number":["#22-15-00393"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells"],"abstract":"<jats:p>Maternal hyperhomocysteinemia causes the disruption of placental blood flow and can lead to serious disturbances in the formation of the offspring\u2019s brain. In the present study, the effects of prenatal hyperhomocysteinemia (PHHC) on the neuronal migration, neural tissue maturation, and the expression of signaling molecules in the rat fetal brain were described. Maternal hyperhomocysteinemia was induced in female rats by per os administration of 0.15% aqueous methionine solution in the period of days 4\u201321 of pregnancy. Behavioral tests revealed a delay in PHHC male pups maturing. Ultrastructure of both cortical and hippocampus tissue demonstrated the features of the developmental delay. PHHC was shown to disturb both generation and radial migration of neuroblasts into the cortical plate. Elevated Bdnf expression, together with changes in proBDNF\/mBDNF balance, might affect neuronal cell viability, positioning, and maturation in PHHC pups. Reduced Kdr gene expression and the content of SEMA3E might lead to impaired brain development. In the brain tissue of E20 PHHC fetuses, the content of the procaspase-8 was decreased, and the activity level of the caspase-3 was increased; this may indicate the development of apoptosis. PHHC disturbs the mechanisms of early brain development leading to a delay in brain tissue maturation and formation of the motor reaction of pups.<\/jats:p>","DOI":"10.3390\/cells12010189","type":"journal-article","created":{"date-parts":[[2023,1,3]],"date-time":"2023-01-03T02:05:53Z","timestamp":1672711553000},"page":"189","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Maternal Hyperhomocysteinemia Disturbs the Mechanisms of Embryonic Brain Development and Its Maturation in Early Postnatal Ontogenesis"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0601-2358","authenticated-orcid":false,"given":"Dmitrii S.","family":"Vasilev","sequence":"first","affiliation":[{"name":"I. M. 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