{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T23:17:24Z","timestamp":1792019844860,"version":"4.4.0"},"reference-count":16,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2023,1,7]],"date-time":"2023-01-07T00:00:00Z","timestamp":1673049600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Fondation pour la Recherche en Sant\u00e9 Respiratoire","award":["FR2018"],"award-info":[{"award-number":["FR2018"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells"],"abstract":"<jats:p>Background: Acute respiratory distress syndrome (ARDS) is a life-threatening condition resulting from acute pulmonary inflammation. However, no specific treatment for ARDS has yet been developed. Previous findings suggest that lung injuries related to ARDS could be regulated by endocan (Esm-1). The aim of this study was to evaluate the potential efficiency of endocan in the treatment of ARDS. Methods: We first compared the features of acute pulmonary inflammation and the severity of hypoxemia in a tracheal LPS-induced acute lung injury (ALI) model performed in knockout (Esm1\u2212\/\u2212) and wild type (WT) littermate C57Bl\/6 mice. Next, we assessed the effects of a continuous infusion of glycosylated murine endocan in our ALI model in Esm1\u2212\/\u2212 mice. Results: In our ALI model, we report higher alveolar leukocytes (p &lt; 0.001), neutrophils (p &lt; 0.001), and MPO (p &lt; 0.001), and lower blood oxygenation (p &lt; 0.001) in Esm1\u2212\/\u2212 mice compared to WT mice. Continuous delivery of glycosylated murine endocan after LPS-induced ALI resulted in decreased alveolar leukocytes (p = 0.012) and neutrophils (p = 0.012), higher blood oxygenation levels (p &lt; 0.001), and reduced histological lung injury (p = 0.04), compared to mice treated with PBS. Conclusions: Endocan appears to be an effective treatment in an ARDS-like model in C57Bl\/6 mice.<\/jats:p>","DOI":"10.3390\/cells12020257","type":"journal-article","created":{"date-parts":[[2023,1,9]],"date-time":"2023-01-09T03:25:58Z","timestamp":1673234758000},"page":"257","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Evaluation of Endocan as a Treatment for Acute Inflammatory Respiratory Failure"],"prefix":"10.3390","volume":"12","author":[{"given":"Maxence","family":"Hureau","sequence":"first","affiliation":[{"name":"Univ. de Lille, CNRS, Inserm, CHU Lille, Institut Pasteur de Lille, U1019-UMR9017-CIIL-Centre d\u2019Infection et d\u2019Immunit\u00e9 de Lille, CHU Lille, Surgical Critical Care, Department of Anesthesiology and Critical Care, F-59000 Lille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucie","family":"Portier","sequence":"additional","affiliation":[{"name":"Biothelis, F-59000 Lille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M\u00e9line","family":"Prin","sequence":"additional","affiliation":[{"name":"Centre Hospitalier de Valenciennes, Laboratoire d\u2019Anatomopathologie, F-59300 Valenciennes, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patricia","family":"de Nadai","sequence":"additional","affiliation":[{"name":"Univ. Lille, CNRS, Inserm, CHU Lille, Institut Pasteur de Lille, U1019-UMR9017-CIIL-Centre d\u2019Infection et d\u2019Immunit\u00e9 de Lille, F-59000 Lille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joanne","family":"Balsamelli","sequence":"additional","affiliation":[{"name":"Univ. Lille, CNRS, Inserm, CHU Lille, Institut Pasteur de Lille, U1019-UMR9017-CIIL-Centre d\u2019Infection et d\u2019Immunit\u00e9 de Lille, F-59000 Lille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1579-2763","authenticated-orcid":false,"given":"Anne","family":"Tsicopoulos","sequence":"additional","affiliation":[{"name":"Univ. Lille, CNRS, Inserm, CHU Lille, Institut Pasteur de Lille, U1019-UMR9017-CIIL-Centre d\u2019Infection et d\u2019Immunit\u00e9 de Lille, CHU Lille, Service de Pneumologie et Immuno-Allergologie, Centre de Comp\u00e9tence pour les Maladies Pulmonaires Rares, F-59000 Lille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"Mathieu","sequence":"additional","affiliation":[{"name":"Univ. Lille, CNRS, Inserm, CHU Lille, Institut Pasteur de Lille, U1019-UMR9017-CIIL-Centre d\u2019Infection et d\u2019Immunit\u00e9 de Lille, CHU Lille, P\u00f4le de M\u00e9decine Intensive\u2014R\u00e9animation, F-59000 Lille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6434-0344","authenticated-orcid":false,"given":"Philippe","family":"Lassalle","sequence":"additional","affiliation":[{"name":"Biothelis, F-59000 Lille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bogdan","family":"Grigoriu","sequence":"additional","affiliation":[{"name":"Service des Soins Intensifs et Urgences Oncologiques, Institut Jules Bordet, Universit\u00e9 Libre de Bruxelles (ULB), 1050 Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandre","family":"Gaudet","sequence":"additional","affiliation":[{"name":"Univ. Lille, CNRS, Inserm, CHU Lille, Institut Pasteur de Lille, U1019-UMR9017-CIIL-Centre d\u2019Infection et d\u2019Immunit\u00e9 de Lille, CHU Lille, P\u00f4le de M\u00e9decine Intensive\u2014R\u00e9animation, F-59000 Lille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nathalie","family":"De Freitas Caires","sequence":"additional","affiliation":[{"name":"Biothelis, F-59000 Lille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,1,7]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"788","DOI":"10.1001\/jama.2016.0291","article-title":"Epidemiology, Patterns of Care, and Mortality for Patients With Acute Respiratory Distress Syndrome in Intensive Care Units in 50 Countries","volume":"315","author":"Bellani","year":"2016","journal-title":"JAMA"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1334","DOI":"10.1056\/NEJM200005043421806","article-title":"The Acute Respiratory Distress Syndrome","volume":"342","author":"Ware","year":"2000","journal-title":"N. Engl. J. Med."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"678","DOI":"10.1038\/nri2156","article-title":"Getting to the Site of Inflammation: The Leukocyte Adhesion Cascade Updated","volume":"7","author":"Ley","year":"2007","journal-title":"Nat. Rev. Immunol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"20458","DOI":"10.1074\/jbc.271.34.20458","article-title":"ESM-1 Is a Novel Human Endothelial Cell-Specific Molecule Expressed in Lung and Regulated by Cytokines","volume":"271","author":"Lassalle","year":"1996","journal-title":"J. Biol. Chem."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"668","DOI":"10.1152\/japplphysiol.00337.2019","article-title":"Endocan Regulates Acute Lung Inflammation through Control of Leukocyte Diapedesis","volume":"127","author":"Gaudet","year":"2019","journal-title":"J. Appl. Physiol."},{"key":"ref_6","first-page":"e69","article-title":"Highlight on Mouse Endocan","volume":"116","author":"Lassalle","year":"2015","journal-title":"Circ. Res."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"280","DOI":"10.1186\/s13054-018-2222-7","article-title":"Endocan, Sepsis, Pneumonia, and Acute Respiratory Distress Syndrome","volume":"22","author":"Gaudet","year":"2018","journal-title":"Crit. Care"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1186\/s13613-017-0311-4","article-title":"Endocan as an Early Biomarker of Severity in Patients with Acute Respiratory Distress Syndrome","volume":"7","author":"Orbegozo","year":"2017","journal-title":"Ann. Intensive Care"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1016\/j.cyto.2015.02.013","article-title":"Evaluation of Endothelial Biomarkers as Predictors of Organ Failures in Septic Shock Patients","volume":"73","author":"Palud","year":"2015","journal-title":"Cytokine"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1016\/j.jcrc.2018.06.018","article-title":"Low Endocan Levels Are Predictive of Acute Respiratory Distress Syndrome in Severe Sepsis and Septic Shock","volume":"47","author":"Gaudet","year":"2018","journal-title":"J. Crit. Care"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1016\/j.jim.2012.02.009","article-title":"Development of Monoclonal Antibodies and ELISA Specific for the Mouse Vascular Endocan","volume":"378","author":"Depontieu","year":"2012","journal-title":"J. Immunol. 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Biol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"2725","DOI":"10.18632\/oncotarget.2614","article-title":"The Non Glycanated Endocan Polypeptide Slows Tumor Growth by Inducing Stromal Inflammatory Reaction","volume":"6","author":"Yassine","year":"2015","journal-title":"Oncotarget"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1016\/j.lfs.2018.04.005","article-title":"A Novel Role of Endocan in Alleviating LPS-Induced Acute Lung Injury","volume":"202","author":"Zhang","year":"2018","journal-title":"Life Sci."}],"container-title":["Cells"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2073-4409\/12\/2\/257\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T18:03:03Z","timestamp":1760119383000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2073-4409\/12\/2\/257"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,1,7]]},"references-count":16,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2023,1]]}},"alternative-id":["cells12020257"],"URL":"https:\/\/doi.org\/10.3390\/cells12020257","relation":{},"ISSN":["2073-4409"],"issn-type":[{"value":"2073-4409","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,1,7]]}}}