{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T14:54:42Z","timestamp":1781103282676,"version":"3.54.1"},"reference-count":70,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2023,1,9]],"date-time":"2023-01-09T00:00:00Z","timestamp":1673222400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004281","name":"National Science Centre","doi-asserted-by":"publisher","award":["2016\/20\/S\/NZ7\/00424"],"award-info":[{"award-number":["2016\/20\/S\/NZ7\/00424"]}],"id":[{"id":"10.13039\/501100004281","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells"],"abstract":"<jats:p>The G-protein coupled receptor 39 (GPR39) is gaining increasing attention as a target for future drugs, yet there are gaps in the understanding of its pharmacology. Zinc is an endogenous agonist or an allosteric modulator, while TC-G 1008 is a synthetic, small molecule agonist. Zinc is also a positive allosteric modulator for the activity of TC-G 1008 at GPR39. Activation of GPR39 by TC-G 1008 facilitated the development of epileptogenesis in the pentylenetetrazole (PTZ)-induced kindling model of epilepsy. Congruently, TC-G 1008 decreased the seizure threshold in the maximal electroshock seizure threshold (MEST) test. Here, we investigated the effects of TC-G 1008 under the condition of zinc deficiency. Mice were fed a zinc-adequate diet (ZnA, 50 mg Zn\/kg) or a zinc-deficient diet (ZnD, 3 mg Zn\/kg) for 4 weeks. Following 4 weeks of dietary zinc restriction, TC-G 1008 was administered as a single dose and the MEST test was performed. Additional groups of mice began the PTZ-kindling model during which TC-G 1008 was administered repeatedly and the diet was continued. TC-G 1008 administered acutely decreased the seizure threshold in the MEST test in mice fed the ZnD diet but not in mice fed the ZnA diet. TC-G 1008 administered chronically increased the maximal seizure severity and the percentage of fully kindled mice in those fed the ZnA diet, but not in mice fed the ZnD diet. Our data showed that the amount of zinc in a diet is a factor contributing to the effects of TC-G 1008 in vivo.<\/jats:p>","DOI":"10.3390\/cells12020264","type":"journal-article","created":{"date-parts":[[2023,1,9]],"date-time":"2023-01-09T05:57:14Z","timestamp":1673243834000},"page":"264","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Dietary Zinc Differentially Regulates the Effects of the GPR39 Receptor Agonist, TC-G 1008, in the Maximal Electroshock Seizure Test and Pentylenetetrazole-Kindling Model of Epilepsy"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2700-8155","authenticated-orcid":false,"given":"Urszula","family":"Doboszewska","sequence":"first","affiliation":[{"name":"Department of Animal Physiology and Pharmacology, Institute of Biological Sciences, Maria Curie-Sk\u0142odowska University, Akademicka 19, PL 20-033 Lublin, Poland"},{"name":"Department of Pharmacobiology, Jagiellonian University Medical College, Medyczna 9, PL 30-688 Krak\u00f3w, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7706-2080","authenticated-orcid":false,"given":"Katarzyna","family":"Soca\u0142a","sequence":"additional","affiliation":[{"name":"Department of Animal Physiology and Pharmacology, Institute of Biological Sciences, Maria Curie-Sk\u0142odowska University, Akademicka 19, PL 20-033 Lublin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mateusz","family":"Pier\u00f3g","sequence":"additional","affiliation":[{"name":"Department of Animal Physiology and Pharmacology, Institute of Biological Sciences, Maria Curie-Sk\u0142odowska University, Akademicka 19, PL 20-033 Lublin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9446-7343","authenticated-orcid":false,"given":"Dorota","family":"Nieoczym","sequence":"additional","affiliation":[{"name":"Department of Animal Physiology and Pharmacology, Institute of Biological Sciences, Maria Curie-Sk\u0142odowska University, Akademicka 19, PL 20-033 Lublin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5945-1047","authenticated-orcid":false,"given":"Jan","family":"Sawicki","sequence":"additional","affiliation":[{"name":"Department of Analytical Chemistry, Medical University of Lublin, Chod\u017aki 4A, PL 20-093 Lublin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7385-2875","authenticated-orcid":false,"given":"Adam","family":"Sajn\u00f3g","sequence":"additional","affiliation":[{"name":"Department of Trace Analysis, Adam Mickiewicz University, Uniwersytetu Poznanskiego 8, PL 61-614 Poznan, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6863-7951","authenticated-orcid":false,"given":"Bernadeta","family":"Szewczyk","sequence":"additional","affiliation":[{"name":"Department of Neurobiology, Maj Institute of Pharmacology, Polish Academy of Sciences, Smetna 12, PL 31-343 Krakow, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katarzyna","family":"Mlyniec","sequence":"additional","affiliation":[{"name":"Department of Pharmacobiology, Jagiellonian University Medical College, Medyczna 9, PL 30-688 Krak\u00f3w, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9346-6325","authenticated-orcid":false,"given":"Ireneusz","family":"Sowa","sequence":"additional","affiliation":[{"name":"Department of Analytical Chemistry, Medical University of Lublin, Chod\u017aki 4A, PL 20-093 Lublin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1186-3552","authenticated-orcid":false,"given":"Danuta","family":"Bara\u0142kiewicz","sequence":"additional","affiliation":[{"name":"Department of Trace Analysis, Adam Mickiewicz University, Uniwersytetu Poznanskiego 8, PL 61-614 Poznan, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5389-0241","authenticated-orcid":false,"given":"Piotr","family":"Wla\u017a","sequence":"additional","affiliation":[{"name":"Department of Animal Physiology and Pharmacology, Institute of Biological Sciences, Maria Curie-Sk\u0142odowska University, Akademicka 19, PL 20-033 Lublin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,1,9]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1124\/mol.117.111062","article-title":"G protein-coupled receptors as targets for approved drugs: How many targets and how many drugs?","volume":"93","author":"Sriram","year":"2018","journal-title":"Mol. 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