{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,22]],"date-time":"2026-01-22T03:34:30Z","timestamp":1769052870142,"version":"3.49.0"},"reference-count":39,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2023,2,2]],"date-time":"2023-02-02T00:00:00Z","timestamp":1675296000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003141","name":"Consejo Nacional de Ciencia y Tecnolog\u00eda (CONACYT), MEXICO","doi-asserted-by":"publisher","award":["283363"],"award-info":[{"award-number":["283363"]}],"id":[{"id":"10.13039\/501100003141","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003141","name":"Consejo Nacional de Ciencia y Tecnolog\u00eda (CONACYT), MEXICO","doi-asserted-by":"publisher","award":["316650"],"award-info":[{"award-number":["316650"]}],"id":[{"id":"10.13039\/501100003141","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells"],"abstract":"<jats:p>Caveolae-associated signaling toward mitochondria contributes to the cardioprotective mechanisms against ischemia-reperfusion (I\/R) injury induced by ischemic postconditioning. In this work, we evaluated the role that the actin-cytoskeleton network exerts on caveolae-mitochondria communication during postconditioning. Isolated rat hearts subjected to I\/R and to postconditioning were treated with latrunculin A, a cytoskeleton disruptor. Cardiac function was compared between these hearts and those exposed only to I\/R and to the cardioprotective maneuver. Caveolae and mitochondria structures were determined by electron microscopy and maintenance of the actin-cytoskeleton was evaluated by phalloidin staining. Caveolin-3 and other putative caveolae-conforming proteins were detected by immunoblot analysis. Co-expression of caveolin-3 and actin was evaluated both in lipid raft fractions and in heart tissue from the different groups. Mitochondrial function was assessed by respirometry and correlated with cholesterol levels. Treatment with latrunculin A abolishes the cardioprotective postconditioning effect, inducing morphological and structural changes in cardiac tissue, reducing F-actin staining and diminishing caveolae formation. Latrunculin A administration to post-conditioned hearts decreases the interaction between caveolae-forming proteins, the co-localization of caveolin with actin and inhibits oxygen consumption rates in both subsarcolemmal and interfibrillar mitochondria. We conclude that actin-cytoskeleton drives caveolae signaling to mitochondria during postconditioning, supporting their functional integrity and contributing to cardiac adaption against reperfusion injury.<\/jats:p>","DOI":"10.3390\/cells12030492","type":"journal-article","created":{"date-parts":[[2023,2,3]],"date-time":"2023-02-03T03:04:23Z","timestamp":1675393463000},"page":"492","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Actin-Cytoskeleton Drives Caveolae Signaling to Mitochondria during Postconditioning"],"prefix":"10.3390","volume":"12","author":[{"given":"Francisco","family":"Correa","sequence":"first","affiliation":[{"name":"Departamento de Biomedicina Cardiovascular, Instituto Nacional de Cardiolog\u00eda Ignacio Ch\u00e1vez, Juan Badiano No. 1, Colonia Secci\u00f3n XVI, Mexico City 14080, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cristina","family":"Enr\u00edquez-Cortina","sequence":"additional","affiliation":[{"name":"Departamento de Biomedicina Cardiovascular, Instituto Nacional de Cardiolog\u00eda Ignacio Ch\u00e1vez, Juan Badiano No. 1, Colonia Secci\u00f3n XVI, Mexico City 14080, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8409-7967","authenticated-orcid":false,"given":"Alejandro","family":"Silva-Palacios","sequence":"additional","affiliation":[{"name":"Departamento de Biomedicina Cardiovascular, Instituto Nacional de Cardiolog\u00eda Ignacio Ch\u00e1vez, Juan Badiano No. 1, Colonia Secci\u00f3n XVI, Mexico City 14080, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nadia","family":"Rom\u00e1n-Anguiano","sequence":"additional","affiliation":[{"name":"Departamento de Biomedicina Cardiovascular, Instituto Nacional de Cardiolog\u00eda Ignacio Ch\u00e1vez, Juan Badiano No. 1, Colonia Secci\u00f3n XVI, Mexico City 14080, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aurora","family":"Gil-Hern\u00e1ndez","sequence":"additional","affiliation":[{"name":"Departamento de Biomedicina Cardiovascular, Instituto Nacional de Cardiolog\u00eda Ignacio Ch\u00e1vez, Juan Badiano No. 1, Colonia Secci\u00f3n XVI, Mexico City 14080, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marcos","family":"Ostolga-Chavarr\u00eda","sequence":"additional","affiliation":[{"name":"Departamento de Biomedicina Cardiovascular, Instituto Nacional de Cardiolog\u00eda Ignacio Ch\u00e1vez, Juan Badiano No. 1, Colonia Secci\u00f3n XVI, Mexico City 14080, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elizabeth","family":"Soria-Castro","sequence":"additional","affiliation":[{"name":"Departamento de Biomedicina Cardiovascular, Instituto Nacional de Cardiolog\u00eda Ignacio Ch\u00e1vez, Juan Badiano No. 1, Colonia Secci\u00f3n XVI, Mexico City 14080, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sharik","family":"Hern\u00e1ndez-Rizo","sequence":"additional","affiliation":[{"name":"\u00c1rea de Medicina Experimental y Traslacional, Departamento de Ciencias de la Salud, Universidad Aut\u00f3noma Metropolitana-Iztapalapa, Mexico City 14080, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paola de los","family":"Heros","sequence":"additional","affiliation":[{"name":"Unidad de Investigaci\u00f3n UNAM-INC, Instituto Nacional de Cardiolog\u00eda Ignacio Ch\u00e1vez and Instituto de Investigaciones Biom\u00e9dicas, Universidad Nacional Aut\u00f3noma de M\u00e9xico, Mexico City 14080, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2090-3691","authenticated-orcid":false,"given":"Mar\u00eda","family":"Ch\u00e1vez-Canales","sequence":"additional","affiliation":[{"name":"Unidad de Investigaci\u00f3n UNAM-INC, Instituto Nacional de Cardiolog\u00eda Ignacio Ch\u00e1vez and Instituto de Investigaciones Biom\u00e9dicas, Universidad Nacional Aut\u00f3noma de M\u00e9xico, Mexico City 14080, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1068-316X","authenticated-orcid":false,"given":"Cecilia","family":"Zazueta","sequence":"additional","affiliation":[{"name":"Departamento de Biomedicina Cardiovascular, Instituto Nacional de Cardiolog\u00eda Ignacio Ch\u00e1vez, Juan Badiano No. 1, Colonia Secci\u00f3n XVI, Mexico City 14080, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,2,2]]},"reference":[{"key":"ref_1","first-page":"L843","article-title":"Role of plasmalemmal caveolae in signal transduction","volume":"275","author":"Shaul","year":"1998","journal-title":"Am. 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