{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T01:49:49Z","timestamp":1789609789276,"version":"build-2803163510"},"reference-count":67,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2023,2,10]],"date-time":"2023-02-10T00:00:00Z","timestamp":1675987200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells"],"abstract":"<jats:p>Cocaine is one of the most consumed illegal drugs among (young) adults in the European Union and it exerts various acute and chronic negative effects on psychical and physical health. The central mechanism through which cocaine initially leads to improved performance, followed by addictive behavior, has already been intensively studied and includes effects on the homeostasis of the neurotransmitters dopamine, partly mediated via nicotinic acetylcholine receptors, and serotonin. However, effects on the peripheral nervous system, including the enteric nervous system, are much less understood, though a correlation between cocaine consumption and gastrointestinal symptoms has been reported. The aim of the present study was to gain more information on the effects of cocaine on enteric neuronal functions and the underlying mechanisms. For this purpose, functional experiments using an organ bath, Ussing chamber and neuroimaging techniques were conducted on gastrointestinal tissues from guinea pigs. Key results obtained are that cocaine (1) exhibits a stimulating, non-neuronal effect on gastric antrum motility, (2) acutely (but not chronically) diminishes responses of primary cultured enteric neurons to nicotinic and serotonergic stimulation and (3) reversibly attenuates neuronal-mediated intestinal mucosal secretion. It can be concluded that cocaine, among its central effects, also alters enteric neuronal functions, providing potential explanations for the coexistence of cocaine abuse and gastrointestinal complaints.<\/jats:p>","DOI":"10.3390\/cells12040577","type":"journal-article","created":{"date-parts":[[2023,2,13]],"date-time":"2023-02-13T04:32:58Z","timestamp":1676262778000},"page":"577","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Short- and Long-Term Effects of Cocaine on Enteric Neuronal Functions"],"prefix":"10.3390","volume":"12","author":[{"given":"Kristin","family":"Elfers","sequence":"first","affiliation":[{"name":"Institute for Physiology and Cell Biology, University of Veterinary Medicine Hannover, Foundation, 30173 Hannover, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Menne","sequence":"additional","affiliation":[{"name":"Institute for Physiology and Cell Biology, University of Veterinary Medicine Hannover, Foundation, 30173 Hannover, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9699-5318","authenticated-orcid":false,"given":"Luca","family":"Colnaghi","sequence":"additional","affiliation":[{"name":"Division of Neuroscience, IRCCS San Raffaele Scientific Institute, Via Olgettina 60, 20132 Milano, Italy"},{"name":"School of Medicine, Vita-Salute San Raffaele University, Via Olgettina 58, 20132 Milano, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susanne","family":"Hoppe","sequence":"additional","affiliation":[{"name":"Institute for Physiology and Cell Biology, University of Veterinary Medicine Hannover, Foundation, 30173 Hannover, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7803-3556","authenticated-orcid":false,"given":"Gemma","family":"Mazzuoli-Weber","sequence":"additional","affiliation":[{"name":"Institute for Physiology and Cell Biology, University of Veterinary Medicine Hannover, Foundation, 30173 Hannover, Germany"},{"name":"Center for Systems Neuroscience (ZSN), 30559 Hannover, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,2,10]]},"reference":[{"key":"ref_1","unstructured":"Drugs, E.M.C.F., and Addiction, D. (2022). European Drug Report 2022: Trends and Developments, Publications Office of the European Union."},{"key":"ref_2","unstructured":"Drugs, E.M.C.F., and Addiction, D. (2021). European Drug Report 2021: Trends and Developments, Publications Office of the European Union."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1038\/bjp.2008.77","article-title":"Neuroplasticity in the mesolimbic dopamine system and cocaine addiction","volume":"154","author":"Thomas","year":"2008","journal-title":"Br. J. Pharmacol."},{"key":"ref_4","first-page":"47","article-title":"Amphetamine: Evaluation of d- and l-isomers as releasing agents and uptake inhibitors for 3H-dopamine and 3H-norepinephrine in slices of rat neostriatum and cerebral cortex","volume":"194","author":"Heikkila","year":"1975","journal-title":"J. Pharmacol. Exp. Ther."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1123","DOI":"10.1016\/0006-2952(86)90148-6","article-title":"Structural requirements for cocaine congeners to interact with dopamine and serotonin uptake sites in mouse brain and to induce stereotyped behavior","volume":"35","author":"Reith","year":"1986","journal-title":"Biochem. Pharmacol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1219","DOI":"10.1126\/science.2820058","article-title":"Cocaine receptors on dopamine transporters are related to self-administration of cocaine","volume":"237","author":"Ritz","year":"1987","journal-title":"Science"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"451","DOI":"10.1016\/0165-6147(88)90137-X","article-title":"Neurochemical interactions of cocaine with dopaminergic systems","volume":"9","author":"Galloway","year":"1988","journal-title":"Trends Pharmacol. Sci."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"210","DOI":"10.1016\/j.tips.2004.02.005","article-title":"Historical review: Molecular and cellular mechanisms of opiate and cocaine addiction","volume":"25","author":"Nestler","year":"2004","journal-title":"Trends Pharmacol. Sci."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1111\/j.1749-6632.2009.05144.x","article-title":"Cocaine-induced neuroadaptations in glutamate transmission: Potential therapeutic targets for craving and addiction","volume":"1187","author":"Schmidt","year":"2010","journal-title":"Ann. NY Acad. Sci."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1057","DOI":"10.1523\/JNEUROSCI.4336-13.2014","article-title":"Cocaine dysregulates opioid gating of GABA neurotransmission in the ventral pallidum","volume":"34","author":"Kupchik","year":"2014","journal-title":"J. Neurosci."},{"key":"ref_11","first-page":"19","article-title":"Cocaine inhibition of nicotinic acetylcholine receptors influences dopamine release","volume":"6","author":"Zhang","year":"2014","journal-title":"Front. Synaptic Neurosci."},{"key":"ref_12","first-page":"145","article-title":"The peripheral dopaminergic system: Morphological analysis, functional and clinical applications","volume":"107","author":"Amenta","year":"2002","journal-title":"Ital. J. Anat. Embryol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1385\/JMN:28:1:89","article-title":"GABA receptor-mediated effects in the peripheral nervous system: A cross-interaction with neuroactive steroids","volume":"28","author":"Magnaghi","year":"2006","journal-title":"J. Mol. Neurosci."},{"key":"ref_14","first-page":"3","article-title":"Architecture of enteric neural circuits involved in intestinal motility","volume":"12","author":"Costa","year":"2008","journal-title":"Eur. Rev. Med. Pharmacol. Sci."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1016\/S0950-351X(05)80226-5","article-title":"Gastrointestinal neurotransmitters","volume":"8","author":"McConalogue","year":"1994","journal-title":"Baillieres Clin. Endocrinol. Metab."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"3073","DOI":"10.1523\/JNEUROSCI.19-08-03073.1999","article-title":"Spatiotemporal patterns of activity in an intact mammalian network with single-cell resolution: Optical studies of nicotinic activity in an enteric plexus","volume":"19","author":"Obaid","year":"1999","journal-title":"J. Neurosci."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1016\/S0165-1838(00)00130-2","article-title":"Multiple mechanisms of fast excitatory synaptic transmission in the enteric nervous system","volume":"81","author":"Galligan","year":"2000","journal-title":"J. Auton. Nerv. Syst."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1038\/s41401-019-0276-y","article-title":"Cocaine potently blocks neuronal \u03b1(3)\u03b2(4) nicotinic acetylcholine receptors in SH-SY5Y cells","volume":"41","author":"Ma","year":"2020","journal-title":"Acta Pharmacol. Sin."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1016\/0014-2999(79)90282-6","article-title":"Blockade of serotonin receptors on autonomic neurones by (-)-cocaine and some related compounds","volume":"59","author":"Fozard","year":"1979","journal-title":"Eur. J. Pharmacol."},{"key":"ref_20","first-page":"374","article-title":"Intestinal ischemia caused by cocaine ingestion: Report of two cases","volume":"97","author":"Nalbandian","year":"1985","journal-title":"Surgery"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1495","DOI":"10.1056\/NEJM198612043152327","article-title":"Medical complications of cocaine abuse","volume":"315","author":"Cregler","year":"1986","journal-title":"N. Engl. J. Med."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"909","DOI":"10.1097\/00007611-200009000-00015","article-title":"Cocaine-associated ischemic colitis","volume":"93","author":"Linder","year":"2000","journal-title":"S. Med. J."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"2313","DOI":"10.1007\/s10350-005-0195-5","article-title":"Enterocolitis associated with cocaine use","volume":"48","author":"Ellis","year":"2005","journal-title":"Dis. Colon. Rectum."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1007\/s12519-009-0030-5","article-title":"Massive pan-gastrointestinal bleeding following cocaine use","volume":"5","author":"Gibbons","year":"2009","journal-title":"World J. Pediatr."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1007\/s12640-018-9936-9","article-title":"Alterations in the Gut Microbiota of Rats Chronically Exposed to Volatilized Cocaine and Its Active Adulterants Caffeine and Phenacetin","volume":"35","author":"Scorza","year":"2019","journal-title":"Neurotox. Res."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"12187","DOI":"10.1038\/s41598-019-48428-2","article-title":"Cocaine Induces Inflammatory Gut Milieu by Compromising the Mucosal Barrier Integrity and Altering the Gut Microbiota Colonization","volume":"9","author":"Chivero","year":"2019","journal-title":"Sci. Rep."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1620","DOI":"10.3389\/fmicb.2017.01620","article-title":"Gut Microbiota Analysis in Rats with Methamphetamine-Induced Conditioned Place Preference","volume":"8","author":"Ning","year":"2017","journal-title":"Front. Microbiol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"35455","DOI":"10.1038\/srep35455","article-title":"Alterations of the Host Microbiome Affect Behavioral Responses to Cocaine","volume":"6","author":"Kiraly","year":"2016","journal-title":"Sci. Rep."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"342","DOI":"10.3389\/fncel.2015.00342","article-title":"Mechanical stress activates neurites and somata of myenteric neurons","volume":"9","author":"Kugler","year":"2015","journal-title":"Front. Cell. Neurosci."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1111\/j.1748-1716.1951.tb00800.x","article-title":"Active transport of sodium as the source of electric current in the short-circuited isolated frog skin","volume":"23","author":"Ussing","year":"1951","journal-title":"Acta Physiol. Scand."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1196\/annals.1316.006","article-title":"Molecular mechanisms underlying the rewarding effects of cocaine","volume":"1025","author":"Hall","year":"2004","journal-title":"Ann. NY Acad. Sci."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1151\/spp05314","article-title":"The neurobiology of cocaine addiction","volume":"3","author":"Nestler","year":"2005","journal-title":"Sci. Pract. Perspect."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1177\/014107680609900203","article-title":"Life threatening abdominal complications following cocaine abuse","volume":"99","author":"Tiwari","year":"2006","journal-title":"J. R. Soc. Med."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"531","DOI":"10.1016\/j.bcp.2015.08.083","article-title":"High affinity \u03b13\u03b24 nicotinic acetylcholine receptor ligands AT-1001 and AT-1012 attenuate cocaine-induced conditioned place preference and behavioral sensitization in mice","volume":"97","author":"Khroyan","year":"2015","journal-title":"Biochem. Pharmacol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"889","DOI":"10.1124\/jpet.102.033548","article-title":"Pharmacological properties of nicotinic acetylcholine receptors expressed by guinea pig small intestinal myenteric neurons","volume":"302","author":"Zhou","year":"2002","journal-title":"J. Pharmacol. Exp. Ther."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"176","DOI":"10.1124\/pr.114.009514","article-title":"Serotonin 5-HT2 receptor interactions with dopamine function: Implications for therapeutics in cocaine use disorder","volume":"67","author":"Howell","year":"2015","journal-title":"Pharmacol. Rev."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"482","DOI":"10.1007\/s00213-005-0197-y","article-title":"Contribution of serotonin (5-HT) 5-HT2 receptor subtypes to the discriminative stimulus effects of cocaine in rats","volume":"183","author":"Filip","year":"2006","journal-title":"Psychopharmacology"},{"key":"ref_38","first-page":"576","article-title":"Differential effects of the 5-HT(2A) receptor antagonist M100907 and the 5-HT(2C) receptor antagonist SB242084 on cocaine-induced locomotor activity, cocaine self-administration and cocaine-induced reinstatement of responding","volume":"27","author":"Fletcher","year":"2002","journal-title":"Neuropsychopharmacology"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"1979","DOI":"10.1038\/npp.2009.10","article-title":"Repeated Cocaine Administration Decreases 5-HT2A Receptor-Mediated Serotonergic Enhancement of Synaptic Activity in Rat Medial Prefrontal Cortex","volume":"34","author":"Huang","year":"2009","journal-title":"Neuropsychopharmacology"},{"key":"ref_40","first-page":"1378","article-title":"Pharmacological characterization of a neuronal receptor for 5-hydroxytryptamine in guinea pig ileum with properties similar to the 5-hydroxytryptamine receptor","volume":"252","author":"Craig","year":"1990","journal-title":"J. Pharmacol. Exp. Ther."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"270","DOI":"10.1007\/BF00168686","article-title":"Effects of 5-HT4 receptor stimulation on basal and electrically evoked release of acetylcholine from guinea-pig myenteric plexus","volume":"345","author":"Kilbinger","year":"1992","journal-title":"Naunyn Schmiedebergs Arch. Pharmacol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"863","DOI":"10.1007\/BF01970002","article-title":"The enteric neural receptor for 5-hydroxytryptamine","volume":"41","author":"Gershon","year":"1985","journal-title":"Experientia"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"175","DOI":"10.2174\/1568026013395335","article-title":"From cocaine to ropivacaine: The history of local anesthetic drugs","volume":"1","author":"Ruetsch","year":"2001","journal-title":"Curr. Top. Med. Chem."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"240","DOI":"10.1016\/j.jneumeth.2010.07.038","article-title":"Recordings from human myenteric neurons using voltage-sensitive dyes","volume":"192","author":"Vignali","year":"2010","journal-title":"J. Neurosci. Methods"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"G129","DOI":"10.1152\/ajpgi.00040.2013","article-title":"Morphological, immunocytochemical, and functional characterization of esophageal enteric neurons in primary culture","volume":"305","author":"Dong","year":"2013","journal-title":"Am. J. Physiol. Gastrointest. Liver Physiol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"G748","DOI":"10.1152\/ajpgi.00416.2013","article-title":"VPAC1 receptors regulate intestinal secretion and muscle contractility by activating cholinergic neurons in guinea pig jejunum","volume":"306","author":"Fung","year":"2014","journal-title":"Am. J. Physiol. Gastrointest. Liver Physiol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"741","DOI":"10.1111\/j.1469-7793.2001.00741.x","article-title":"Neural components of distension-evoked secretory responses in the guinea-pig distal colon","volume":"536","author":"Weber","year":"2001","journal-title":"J. Physiol."},{"key":"ref_48","first-page":"G745","article-title":"Effects of neurotransmitter release on mucosal transport in guinea pig ileum","volume":"245","author":"Cooke","year":"1983","journal-title":"Am. J. Physiol."},{"key":"ref_49","first-page":"G263","article-title":"Influence of enteric cholinergic neurons on mucosal transport in guinea pig ileum","volume":"246","author":"Cooke","year":"1984","journal-title":"Am. J. Physiol."},{"key":"ref_50","first-page":"G323","article-title":"Muscarinic receptor subtypes mediating the mucosal response to neural stimulation of guinea pig ileum","volume":"253","author":"Carey","year":"1987","journal-title":"Am. J. Physiol."},{"key":"ref_51","first-page":"G667","article-title":"Epithelial responses evoked by stimulation of submucosal neurons in guinea pig distal colon","volume":"252","author":"Kuwahara","year":"1987","journal-title":"Am. J. Physiol."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1016\/0014-2999(89)90568-2","article-title":"Cholinergic-mediated secretion in the rat colon: Neuronal and epithelial muscarinic responses","volume":"168","author":"Diener","year":"1989","journal-title":"Eur. J. Pharmacol."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"703","DOI":"10.1016\/0016-5085(91)80014-Z","article-title":"Regulation of ion transport in porcine distal colon: Effects of putative neurotransmitters","volume":"100","author":"Traynor","year":"1991","journal-title":"Gastroenterology"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1046\/j.1360-0443.2001.9611038.x","article-title":"Incentive-sensitization and addiction","volume":"96","author":"Robinson","year":"2001","journal-title":"Addiction"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"995","DOI":"10.1016\/0896-6273(93)90213-B","article-title":"Drug addiction: A model for the molecular basis of neural plasticity","volume":"11","author":"Nestler","year":"1993","journal-title":"Neuron"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1038\/35053570","article-title":"Molecular basis of long-term plasticity underlying addiction","volume":"2","author":"Nestler","year":"2001","journal-title":"Nat. Rev. Neurosci."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"1598","DOI":"10.1046\/j.1460-9568.1999.00576.x","article-title":"Alterations in the morphology of dendrites and dendritic spines in the nucleus accumbens and prefrontal cortex following repeated treatment with amphetamine or cocaine","volume":"11","author":"Robinson","year":"1999","journal-title":"Eur. J. Neurosci."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"1322","DOI":"10.1172\/JCI115135","article-title":"Effects of cocaine on excitation-contraction coupling of aortic smooth muscle from the ferret","volume":"87","author":"Egashira","year":"1991","journal-title":"J. Clin. Investig."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"1191","DOI":"10.1161\/01.RES.72.6.1191","article-title":"Extracellular Ca2+-dependent potentiation by cocaine of serotonin- and norepinephrine-induced contractions in rat vascular smooth muscle","volume":"72","author":"Watanabe","year":"1993","journal-title":"Circ. Res."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"459","DOI":"10.1038\/s41398-021-01573-7","article-title":"Ca2+ channel blockade reduces cocaine\u2019s vasoconstriction and neurotoxicity in the prefrontal cortex","volume":"11","author":"Du","year":"2021","journal-title":"Transl. Psychiatry"},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"1260","DOI":"10.1096\/fj.05-5292fje","article-title":"Control of intestinal motility by the Ca(v)1.2 L-type calcium channel in mice","volume":"20","author":"Wegener","year":"2006","journal-title":"FASEB J."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"901","DOI":"10.1152\/physrev.1997.77.4.901","article-title":"Store depletion and calcium influx","volume":"77","author":"Parekh","year":"1997","journal-title":"Physiol. Rev."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"606","DOI":"10.1152\/physrev.1979.59.3.606","article-title":"Mechanisms of action of transmitters and other substances on smooth muscle","volume":"59","author":"Bolton","year":"1979","journal-title":"Physiol. Rev."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"105","DOI":"10.3748\/wjg.v17.i1.105","article-title":"Effect of ginger on gastric motility and symptoms of functional dyspepsia","volume":"17","author":"Hu","year":"2011","journal-title":"World J. Gastroenterol."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"90","DOI":"10.1016\/j.phymed.2006.03.020","article-title":"Region-specific effects of STW 5 (Iberogast) and its components in gastric fundus, corpus and antrum","volume":"13","author":"Schemann","year":"2006","journal-title":"Phytomedicine"},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"765","DOI":"10.1111\/j.1365-2982.2004.00548.x","article-title":"The herbal preparation STW5 (lberogast) has potent and region-specific effects on gastric motility","volume":"16","author":"Hohenester","year":"2004","journal-title":"Neurogastroenterol. Motil."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"e14105","DOI":"10.1111\/nmo.14105","article-title":"Region-specific effects of the cysteine protease papain on gastric motility","volume":"33","author":"Schemann","year":"2021","journal-title":"Neurogastroenterol. Motil."}],"container-title":["Cells"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2073-4409\/12\/4\/577\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T18:30:58Z","timestamp":1760121058000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2073-4409\/12\/4\/577"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,2,10]]},"references-count":67,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2023,2]]}},"alternative-id":["cells12040577"],"URL":"https:\/\/doi.org\/10.3390\/cells12040577","relation":{},"ISSN":["2073-4409"],"issn-type":[{"value":"2073-4409","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,2,10]]}}}