{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T17:10:03Z","timestamp":1790701803615,"version":"4.1.0"},"reference-count":47,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2023,2,11]],"date-time":"2023-02-11T00:00:00Z","timestamp":1676073600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Fondation de l\u2019Avenir","award":["AP-RM-19-001"],"award-info":[{"award-number":["AP-RM-19-001"]}]},{"name":"Fondation de l\u2019Avenir","award":["ANR-20-CE18-0035"],"award-info":[{"award-number":["ANR-20-CE18-0035"]}]},{"name":"Agence Nationale pour la Recherche","award":["AP-RM-19-001"],"award-info":[{"award-number":["AP-RM-19-001"]}]},{"name":"Agence Nationale pour la Recherche","award":["ANR-20-CE18-0035"],"award-info":[{"award-number":["ANR-20-CE18-0035"]}]},{"name":"Universit\u00e9 Paris Est Cr\u00e9teil","award":["AP-RM-19-001"],"award-info":[{"award-number":["AP-RM-19-001"]}]},{"name":"Universit\u00e9 Paris Est Cr\u00e9teil","award":["ANR-20-CE18-0035"],"award-info":[{"award-number":["ANR-20-CE18-0035"]}]},{"name":"Fondation pour la Recherche M\u00e9dicale","award":["AP-RM-19-001"],"award-info":[{"award-number":["AP-RM-19-001"]}]},{"name":"Fondation pour la Recherche M\u00e9dicale","award":["ANR-20-CE18-0035"],"award-info":[{"award-number":["ANR-20-CE18-0035"]}]},{"name":"Region Occitanie (GliMit)","award":["AP-RM-19-001"],"award-info":[{"award-number":["AP-RM-19-001"]}]},{"name":"Region Occitanie (GliMit)","award":["ANR-20-CE18-0035"],"award-info":[{"award-number":["ANR-20-CE18-0035"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells"],"abstract":"<jats:p>Background: The use of mesenchymal stem cells (MSCs) appears to be a promising therapeutic approach for cardiac repair after myocardial infarction. However, clinical trials have revealed the need to improve their therapeutic efficacy. Recent evidence demonstrated that mitochondria undergo spontaneous transfer from damaged cells to MSCs, resulting in the activation of the cytoprotective and pro-angiogenic functions of recipient MSCs. Based on these observations, we investigated whether the preconditioning of MSCs with mitochondria could optimize their therapeutic potential for ischemic heart disease. Methods: Human MSCs were exposed to mitochondria isolated from human fetal cardiomyocytes. After 24 h, the effects of mitochondria preconditioning on the MSCs\u2019 function were analyzed both in vitro and in vivo. Results: We found that cardiac mitochondria-preconditioning improved the proliferation and repair properties of MSCs in vitro. Mechanistically, cardiac mitochondria mediate their stimulatory effects through the production of reactive oxygen species, which trigger their own degradation in recipient MSCs. These effects were further confirmed in vivo, as the mitochondria preconditioning of MSCs potentiated their therapeutic efficacy on cardiac function following their engraftment into infarcted mouse hearts. Conclusions: The preconditioning of MSCs with the artificial transfer of cardiac mitochondria appears to be promising strategy to improve the efficacy of MSC-based cell therapy in ischemic heart disease.<\/jats:p>","DOI":"10.3390\/cells12040582","type":"journal-article","created":{"date-parts":[[2023,2,13]],"date-time":"2023-02-13T04:32:58Z","timestamp":1676262778000},"page":"582","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["Transfer of Cardiac Mitochondria Improves the Therapeutic Efficacy of Mesenchymal Stem Cells in a Preclinical Model of Ischemic Heart Disease"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0183-5953","authenticated-orcid":false,"given":"Marie-Luce","family":"Vignais","sequence":"first","affiliation":[{"name":"Institut de G\u00e9nomique Fonctionnelle, University Montpellier, CNRS, INSERM, 34094 Montpellier, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennyfer","family":"Levoux","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Paris-Est Cr\u00e9teil, INSERM, IMRB, 94010 Cr\u00e9teil, France"},{"name":"Sorbonne Universit\u00e9, Institut de Biologie Paris-Seine (IBPS), CNRS UMR 8256, INSERM U1164, Biological Adaptation and Ageing, 75005 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5837-3916","authenticated-orcid":false,"given":"Pierre","family":"Sicard","sequence":"additional","affiliation":[{"name":"PhyMedExp, Inserm, CNRS, University of Montpellier, 34295 Montpellier, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Khattar","family":"Khattar","sequence":"additional","affiliation":[{"name":"Institut de G\u00e9nomique Fonctionnelle, University Montpellier, CNRS, INSERM, 34094 Montpellier, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6751-7042","authenticated-orcid":false,"given":"Catherine","family":"Lozza","sequence":"additional","affiliation":[{"name":"PhyMedExp, Inserm, CNRS, University of Montpellier, 34295 Montpellier, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marianne","family":"Gervais","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Paris-Est Cr\u00e9teil, INSERM, IMRB, 94010 Cr\u00e9teil, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Safia","family":"Mezhoud","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Paris-Est Cr\u00e9teil, INSERM, IMRB, 94010 Cr\u00e9teil, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean","family":"Nakhle","sequence":"additional","affiliation":[{"name":"Institut de G\u00e9nomique Fonctionnelle, University Montpellier, CNRS, INSERM, 34094 Montpellier, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1270-1472","authenticated-orcid":false,"given":"Frederic","family":"Relaix","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Paris-Est Cr\u00e9teil, INSERM, IMRB, 94010 Cr\u00e9teil, France"},{"name":"\u00c9cole Nationale V\u00e9t\u00e9rinaire d\u2019Alfort, IMRB, 94700 Maisons-Alfort, France"},{"name":"APHP, H\u00f4pitaux Universitaires Henri Mondor & Centre de R\u00e9f\u00e9rence des Maladies Neuromusculaires GNMH, 94000 Cr\u00e9teil, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Onnik","family":"Agbulut","sequence":"additional","affiliation":[{"name":"Sorbonne Universit\u00e9, Institut de Biologie Paris-Seine (IBPS), CNRS UMR 8256, INSERM U1164, Biological Adaptation and Ageing, 75005 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeremy","family":"Fauconnier","sequence":"additional","affiliation":[{"name":"PhyMedExp, Inserm, CNRS, University of Montpellier, 34295 Montpellier, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5557-8975","authenticated-orcid":false,"given":"Anne-Marie","family":"Rodriguez","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Paris-Est Cr\u00e9teil, INSERM, IMRB, 94010 Cr\u00e9teil, France"},{"name":"Sorbonne Universit\u00e9, Institut de Biologie Paris-Seine (IBPS), CNRS UMR 8256, INSERM U1164, Biological Adaptation and Ageing, 75005 Paris, France"},{"name":"APHP, H\u00f4pitaux Universitaires Henri Mondor & Centre de R\u00e9f\u00e9rence des Maladies Neuromusculaires GNMH, 94000 Cr\u00e9teil, 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