{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T17:29:28Z","timestamp":1780594168731,"version":"3.54.1"},"reference-count":39,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2023,2,11]],"date-time":"2023-02-11T00:00:00Z","timestamp":1676073600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Institute for Social Security and Services for State Workers","award":["294.2016"],"award-info":[{"award-number":["294.2016"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells"],"abstract":"<jats:p>Background: Critical limb ischemia represents an advanced stage of peripheral arterial disease. Angioplasty improves blood flow to the limb; however, some patients progress irreversibly to lower limb amputation. Few studies have explored the predictive potential of biomarkers during postangioplasty outcomes. Aim: To evaluate the behavior of endothelial progenitor cells in patients with critical limb ischemia, in relation to their postangioplasty outcome. Methods: Twenty patients with critical limb ischemia, candidates for angioplasty, were enrolled. Flow-mediated dilation, as well as endothelial progenitor cells (subpopulations CD45+\/CD34+\/CD133+\/CD184+ and CD45+\/CD\/34+\/KDR[VEGFR-2]+ estimated by flow cytometry) from blood flow close to vascular damage, were evaluated before and after angioplasty. Association with lower limb amputation during a 30-day follow-up was analyzed. Results: Endothelial progenitor cells were related with flow-mediated dilation. A higher number of baseline EPCs CD45+CD34+KDR+, as well as an impaired reactivity of endothelial progenitor cells CD45+CD34+CD133+CD184+ after angioplasty, were observed in cases further undergoing major limb amputation, with a significant discrimination ability and risk (0.75, specificity 0.83 and RR 4.5 p &lt; 0.05). Conclusions: Endothelial progenitor cells were related with endothelial dysfunction, whereas a higher baseline number of the subpopulation CD45+CD34+KDR+, as well as an impaired reactivity of subpopulation CD45+CD34+CD133+CD184+ after angioplasty, showed a predictive ability for major limb amputation in patients with critical limb ischemia.<\/jats:p>","DOI":"10.3390\/cells12040584","type":"journal-article","created":{"date-parts":[[2023,2,13]],"date-time":"2023-02-13T04:32:58Z","timestamp":1676262778000},"page":"584","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Endothelial Progenitor Cells May Be Related to Major Amputation after Angioplasty in Patients with Critical Limb Ischemia"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2121-8194","authenticated-orcid":false,"given":"Daniel","family":"Santill\u00e1n-Cortez","sequence":"first","affiliation":[{"name":"Experimental Metabolism and Clinical Research Laboratory, Clinical Research Department, Division of Biomedical Research, Centro M\u00e9dico Nacional \u201c20 de Noviembre\u201d, Instituto de Seguridad y Servicios Sociales para los Trabajadores del Estado, Mexico City P.O. 03100, Mexico"},{"name":"Vascular Surgery and Angiology Department, Centro M\u00e9dico Nacional \u201c20 de Noviembre\u201d, Instituto de Seguridad y Servicios Sociales para los Trabajadores del Estado, Mexico City P.O. 03100, Mexico"},{"name":"Regenerative Medicine and Tissue Engineering Laboratory, Coordination of Research, Centro M\u00e9dico Nacional \u201c20 de Noviembre\u201d, Instituto de Seguridad y Servicios Sociales para los Trabajadores del Estado, Mexico City P.O. 03100, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eduardo","family":"Vera-G\u00f3mez","sequence":"additional","affiliation":[{"name":"Experimental Metabolism and Clinical Research Laboratory, Clinical Research Department, Division of Biomedical Research, Centro M\u00e9dico Nacional \u201c20 de Noviembre\u201d, Instituto de Seguridad y Servicios Sociales para los Trabajadores del Estado, Mexico City P.O. 03100, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alejandro","family":"Hern\u00e1ndez-Patricio","sequence":"additional","affiliation":[{"name":"Experimental Metabolism and Clinical Research Laboratory, Clinical Research Department, Division of Biomedical Research, Centro M\u00e9dico Nacional \u201c20 de Noviembre\u201d, Instituto de Seguridad y Servicios Sociales para los Trabajadores del Estado, Mexico City P.O. 03100, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Atz\u00edn Su\u00e1","family":"Ru\u00edz-Hern\u00e1ndez","sequence":"additional","affiliation":[{"name":"Experimental Metabolism and Clinical Research Laboratory, Clinical Research Department, Division of Biomedical Research, Centro M\u00e9dico Nacional \u201c20 de Noviembre\u201d, Instituto de Seguridad y Servicios Sociales para los Trabajadores del Estado, Mexico City P.O. 03100, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan Ariel","family":"Guti\u00e9rrez-Buend\u00eda","sequence":"additional","affiliation":[{"name":"Experimental Metabolism and Clinical Research Laboratory, Clinical Research Department, Division of Biomedical Research, Centro M\u00e9dico Nacional \u201c20 de Noviembre\u201d, Instituto de Seguridad y Servicios Sociales para los Trabajadores del Estado, Mexico City P.O. 03100, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karen","family":"De la Vega-Moreno","sequence":"additional","affiliation":[{"name":"Experimental Metabolism and Clinical Research Laboratory, Clinical Research Department, Division of Biomedical Research, Centro M\u00e9dico Nacional \u201c20 de Noviembre\u201d, Instituto de Seguridad y Servicios Sociales para los Trabajadores del Estado, Mexico City P.O. 03100, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasser Alberto","family":"Rizo-Garc\u00eda","sequence":"additional","affiliation":[{"name":"Vascular Surgery and Angiology Department, Centro M\u00e9dico Nacional \u201c20 de Noviembre\u201d, Instituto de Seguridad y Servicios Sociales para los Trabajadores del Estado, Mexico City P.O. 03100, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oscar Antonio","family":"Loman-Zu\u00f1iga","sequence":"additional","affiliation":[{"name":"Vascular Surgery and Angiology Department, Centro M\u00e9dico Nacional \u201c20 de Noviembre\u201d, Instituto de Seguridad y Servicios Sociales para los Trabajadores del Estado, Mexico City P.O. 03100, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ignacio","family":"Escotto-S\u00e1nchez","sequence":"additional","affiliation":[{"name":"Vascular Surgery and Angiology Department, Centro M\u00e9dico Nacional \u201c20 de Noviembre\u201d, Instituto de Seguridad y Servicios Sociales para los Trabajadores del Estado, Mexico City P.O. 03100, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan Miguel","family":"Rodr\u00edguez-Trejo","sequence":"additional","affiliation":[{"name":"Vascular Surgery and Angiology Department, Centro M\u00e9dico Nacional \u201c20 de Noviembre\u201d, Instituto de Seguridad y Servicios Sociales para los Trabajadores del Estado, Mexico City P.O. 03100, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mario Antonio","family":"T\u00e9llez-Gonz\u00e1lez","sequence":"additional","affiliation":[{"name":"Regenerative Medicine and Tissue Engineering Laboratory, Coordination of Research, Centro M\u00e9dico Nacional \u201c20 de Noviembre\u201d, Instituto de Seguridad y Servicios Sociales para los Trabajadores del Estado, Mexico City P.O. 03100, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian Gabriel","family":"Toledo-Lozano","sequence":"additional","affiliation":[{"name":"Experimental Metabolism and Clinical Research Laboratory, Clinical Research Department, Division of Biomedical Research, Centro M\u00e9dico Nacional \u201c20 de Noviembre\u201d, Instituto de Seguridad y Servicios Sociales para los Trabajadores del Estado, Mexico City P.O. 03100, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tania","family":"Ortega-Rosas","sequence":"additional","affiliation":[{"name":"Experimental Metabolism and Clinical Research Laboratory, Clinical Research Department, Division of Biomedical Research, Centro M\u00e9dico Nacional \u201c20 de Noviembre\u201d, Instituto de Seguridad y Servicios Sociales para los Trabajadores del Estado, Mexico City P.O. 03100, 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Antonio","family":"Su\u00e1rez-Cuenca","sequence":"additional","affiliation":[{"name":"Experimental Metabolism and Clinical Research Laboratory, Clinical Research Department, Division of Biomedical Research, Centro M\u00e9dico Nacional \u201c20 de Noviembre\u201d, Instituto de Seguridad y Servicios Sociales para los Trabajadores del Estado, Mexico City P.O. 03100, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,2,11]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"378","DOI":"10.1055\/s-0034-1393976","article-title":"Overview of Classification Systems in Peripheral Artery Disease","volume":"31","author":"Hardman","year":"2014","journal-title":"Semin. 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