{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T04:36:52Z","timestamp":1787027812833,"version":"build-2736575974"},"reference-count":53,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2023,2,28]],"date-time":"2023-02-28T00:00:00Z","timestamp":1677542400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004663","name":"Ministry of Science and Technology","doi-asserted-by":"publisher","award":["MOST 109-2628-B-192 -001-"],"award-info":[{"award-number":["MOST 109-2628-B-192 -001-"]}],"id":[{"id":"10.13039\/501100004663","id-type":"DOI","asserted-by":"publisher"}]},{"name":"GenMont Biotech (Taiwan)","award":["MOST 109-2628-B-192 -001-"],"award-info":[{"award-number":["MOST 109-2628-B-192 -001-"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells"],"abstract":"<jats:p>Background: Probiotics may facilitate the clinical management of allergic diseases. However, their effects on allergic rhinitis (AR) remain unclear. We examined the efficacy and safety of Lacticaseibacillus paracasei GM-080 in a mouse model of airway hyper-responsiveness (AHR) and in children with perennial AR (PAR) by using a double-blind, prospective, randomized, placebo-controlled design. Methods: The production of interferon (IFN)-\u03b3 and interleukin (IL)-12 was measured by using an enzyme-linked immunosorbent assay. GM-080 safety was evaluated via the whole-genome sequencing (WGS) of virulence genes. An ovalbumin (OVA)-induced AHR mouse model was constructed, and lung inflammation was evaluated by measuring the infiltrating leukocyte content of bronchoalveolar lavage fluid. A clinical trial was conducted with 122 children with PAR who were randomized to receive different doses of GM-080 or the placebo for 3 months, and their AHR symptom severity scores, total nasal symptom scores (TNSSs), and Investigator Global Assessment Scale scores were examined. Results: Among the tested L. paracasei strains, GM-080 induced the highest IFN-\u03b3 and IL-12 levels in mouse splenocytes. WGS analysis revealed the absence of virulence factors or antibiotic-resistance genes in GM-080. The oral administration of GM-080 at 1 \u00d7 107 colony forming units (CFU)\/mouse\/day for 8 weeks alleviated OVA-induced AHR and reduced airway inflammation in mice. In children with PAR, the oral consumption of GM-080 at 2 \u00d7 109 CFU\/day for 3 months ameliorated sneezing and improved Investigator Global Assessment Scale scores significantly. GM-080 consumption led to a nonsignificant decrease in TNSS and also nonsignificantly reduced IgE but increased INF-\u03b3 levels. Conclusion: GM-080 may be used as a nutrient supplement to alleviate airway allergic inflammation.<\/jats:p>","DOI":"10.3390\/cells12050768","type":"journal-article","created":{"date-parts":[[2023,2,28]],"date-time":"2023-02-28T04:28:41Z","timestamp":1677558521000},"page":"768","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":32,"title":["Lacticaseibacillus paracasei GM-080 Ameliorates Allergic Airway Inflammation in Children with Allergic Rhinitis: From an Animal Model to a Double-Blind, Randomized, Placebo-Controlled Trial"],"prefix":"10.3390","volume":"12","author":[{"given":"En-Kwang","family":"Lin","sequence":"first","affiliation":[{"name":"Institute of Clinical Medicine, National Yang Ming Chiao Tung University, Taipei 112304, Taiwan"},{"name":"Division of Colorectal Surgery, Department of Surgery, Wanfang Hospital, Taipei Medical University, Taipei 110301, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2283-1377","authenticated-orcid":false,"given":"Wen-Wei","family":"Chang","sequence":"additional","affiliation":[{"name":"School of Biomedical Sciences, Chung Shan Medical University, Taichung 402306, Taiwan"},{"name":"Department of Medical Research, Chung Shan Medical University Hospital, Taichung 402306, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6158-5207","authenticated-orcid":false,"given":"Jhih-Hua","family":"Jhong","sequence":"additional","affiliation":[{"name":"Department of Medical Research, Hsinchu MacKay Memorial Hospital, Hsinchu 300044, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wan-Hua","family":"Tsai","sequence":"additional","affiliation":[{"name":"Research and Development Department, GenMont Biotech Incorporation, Tainan 741014, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chia-Hsuan","family":"Chou","sequence":"additional","affiliation":[{"name":"Research and Development Department, GenMont Biotech Incorporation, Tainan 741014, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0091-258X","authenticated-orcid":false,"given":"I-Jen","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Taipei Hospital, Ministry of Health and Welfare, New Taipei 242033, Taiwan"},{"name":"School of Medicine, National Yang Ming Chiao Tung University, Taipei 112304, Taiwan"},{"name":"College of Public Health, China Medical University, Taichung 406040, Taiwan"},{"name":"National Institute of Environmental Health Sciences, National Health Research Institutes, Miaoli 350401, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,2,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"878","DOI":"10.1136\/thorax.55.10.878","article-title":"Allergic inflammation in the unified airway: Start with the nose","volume":"55","author":"Lipworth","year":"2000","journal-title":"Thorax"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"6178","DOI":"10.4049\/jimmunol.162.10.6178","article-title":"Th2-induced airway mucus production is dependent on IL-4Ralpha, but not on eosinophils","volume":"162","author":"Cohn","year":"1999","journal-title":"J. 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