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However, it is known that this therapeutic strategy has failed in solid tumors, making the development of immunoadjuvants highly relevant. Recently, we demonstrated that Phoneutria nigriventer spider venom (PnV) components are cytotoxic to glioblastoma (GB) and activate macrophages for an antitumor profile. However, the effects of these molecules on the adaptive immune response have not yet been evaluated. This work aimed to test PnV and its purified fractions in DCs in vitro. For this purpose, bone marrow precursors were collected from male C57BL6 mice, differentiated into DCs and treated with venom or PnV-isolated fractions (F1\u2014molecules &lt; 3 kDa, F2\u20143 to 10 kDa and F3\u2014&gt;10 kDa), with or without costimulation with human GB lysate. The results showed that mainly F1 was able to activate DCs, increasing the activation-dependent surface marker (CD86) and cytokine release (IL-1\u03b2, TNF-\u03b1), in addition to inducing a typical morphology of mature DCs. From the F1 purification, a molecule named LW9 was the most effective, and mass spectrometry showed it to be a peptide. The present findings suggest that this molecule could be an immunoadjuvant with possible application in DC vaccines for the treatment of GB.<\/jats:p>","DOI":"10.3390\/cells12071023","type":"journal-article","created":{"date-parts":[[2023,3,27]],"date-time":"2023-03-27T06:46:19Z","timestamp":1679899579000},"page":"1023","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Isolated Peptide from Spider Venom Modulates Dendritic Cells In Vitro: A Possible Application in Oncoimmunotherapy for Glioblastoma"],"prefix":"10.3390","volume":"12","author":[{"given":"Felipe Cezar","family":"de Mato","sequence":"first","affiliation":[{"name":"Faculdade de Ci\u00eancias Farmac\u00eauticas, Universidade Estadual de Campinas (UNICAMP), Campinas 13083-871, SP, Brazil"},{"name":"Departamento de Biologia Estrutural e Funcional, Instituto de Biologia, UNICAMP, Campinas 13083-862, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6150-3582","authenticated-orcid":false,"given":"Nat\u00e1lia","family":"Barreto","sequence":"additional","affiliation":[{"name":"Faculdade de Ci\u00eancias Farmac\u00eauticas, Universidade Estadual de Campinas (UNICAMP), Campinas 13083-871, SP, Brazil"},{"name":"Departamento de Biologia Estrutural e Funcional, Instituto de Biologia, UNICAMP, Campinas 13083-862, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriel","family":"Cordeiro","sequence":"additional","affiliation":[{"name":"Faculdade de Ci\u00eancias Farmac\u00eauticas, Universidade Estadual de Campinas (UNICAMP), Campinas 13083-871, SP, Brazil"},{"name":"Departamento de Biologia Estrutural e Funcional, Instituto de Biologia, UNICAMP, Campinas 13083-862, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3112-3033","authenticated-orcid":false,"given":"Jaqueline","family":"Munhoz","sequence":"additional","affiliation":[{"name":"Department of Agricultural, Food and Nutritional Sciences (AFNS), University of Alberta, Edmonton, AB T6G 2R3, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0896-0105","authenticated-orcid":false,"given":"Amanda Pires","family":"Bonfanti","sequence":"additional","affiliation":[{"name":"Faculdade de Ci\u00eancias Farmac\u00eauticas, Universidade Estadual de Campinas (UNICAMP), Campinas 13083-871, SP, Brazil"},{"name":"Departamento de Biologia Estrutural e Funcional, Instituto de Biologia, UNICAMP, Campinas 13083-862, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomaz A. A.","family":"da Rocha-e-Silva","sequence":"additional","affiliation":[{"name":"Department of Physiological Sciences, Faculdade Israelita de Ci\u00eancias da Sa\u00fade Albert Einstein, S\u00e3o Paulo 05653-120, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5648-2446","authenticated-orcid":false,"given":"Rafael","family":"Sutti","sequence":"additional","affiliation":[{"name":"Valer Laborat\u00f3rios Eireli, S\u00e3o Paulo 13347-633, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Priscilla B. 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