{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T12:25:26Z","timestamp":1785500726544,"version":"3.56.0"},"reference-count":85,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2023,3,31]],"date-time":"2023-03-31T00:00:00Z","timestamp":1680220800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000024","name":"Canadian Institutes of Health Research","doi-asserted-by":"publisher","award":["MOP-97836"],"award-info":[{"award-number":["MOP-97836"]}],"id":[{"id":"10.13039\/501100000024","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000024","name":"Canadian Institutes of Health Research","doi-asserted-by":"publisher","award":["MOP-123415"],"award-info":[{"award-number":["MOP-123415"]}],"id":[{"id":"10.13039\/501100000024","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000024","name":"Canadian Institutes of Health Research","doi-asserted-by":"publisher","award":["RGPIN-2017-05489"],"award-info":[{"award-number":["RGPIN-2017-05489"]}],"id":[{"id":"10.13039\/501100000024","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"publisher","award":["MOP-97836"],"award-info":[{"award-number":["MOP-97836"]}],"id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"publisher","award":["MOP-123415"],"award-info":[{"award-number":["MOP-123415"]}],"id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"publisher","award":["RGPIN-2017-05489"],"award-info":[{"award-number":["RGPIN-2017-05489"]}],"id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells"],"abstract":"<jats:p>Primary cilia are sensory antennae located at the cell surface which mediate a variety of extracellular signals involved in development, tissue homeostasis, stem cells and cancer. Primary cilia are found in an extensive array of vertebrae cells but can only be generated when cells become quiescent. The small intestinal epithelium is a rapidly self-renewing tissue organized into a functional unit called the crypt\u2013villus axis, containing progenitor and differentiated cells, respectively. Terminally differentiated villus cells are notoriously devoid of primary cilia. We sought to determine if intestinal crypts contain a quiescent cell population that could be identified by the presence of primary cilia. Here we show that primary cilia are detected in a subset of cells located deep in the crypts slightly above a Paneth cell population. Using a normal epithelial proliferative crypt cell model, we show that primary cilia assembly and activity correlate with a quiescent state. These results provide further evidence for the existence of a quiescent cell population in the human small intestine and suggest the potential for new modes of regulation in stem cell dynamics.<\/jats:p>","DOI":"10.3390\/cells12071059","type":"journal-article","created":{"date-parts":[[2023,3,31]],"date-time":"2023-03-31T03:02:01Z","timestamp":1680231721000},"page":"1059","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Primary Cilium Identifies a Quiescent Cell Population in the Human Intestinal Crypt"],"prefix":"10.3390","volume":"12","author":[{"given":"Blanche","family":"S\u00e9nicourt","sequence":"first","affiliation":[{"name":"Laboratory of Intestinal Physiopathology, Faculty of Medicine and Health Sciences, Universit\u00e9 de Shebrooke, Sherbrooke, QC J1H5N4, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriel","family":"Cloutier","sequence":"additional","affiliation":[{"name":"Laboratory of Intestinal Physiopathology, Faculty of Medicine and Health Sciences, Universit\u00e9 de Shebrooke, Sherbrooke, QC J1H5N4, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nuria","family":"Basora","sequence":"additional","affiliation":[{"name":"Laboratory of Intestinal Physiopathology, Faculty of Medicine and Health Sciences, Universit\u00e9 de Shebrooke, Sherbrooke, QC J1H5N4, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4053-2653","authenticated-orcid":false,"given":"Sepideh","family":"Fallah","sequence":"additional","affiliation":[{"name":"Laboratory of Intestinal Physiopathology, Faculty of Medicine and Health Sciences, Universit\u00e9 de Shebrooke, Sherbrooke, QC J1H5N4, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andr\u00e9anne","family":"Laniel","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Physiology, Faculty of Medicine and Health Sciences, Universit\u00e9 de Sherbrooke, Sherbrooke, QC J1H5N4, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7673-5834","authenticated-orcid":false,"given":"Christine","family":"Lavoie","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Physiology, Faculty of Medicine and Health Sciences, Universit\u00e9 de Sherbrooke, Sherbrooke, QC J1H5N4, Canada"},{"name":"Centre de Recherche du Centre Hospitalier Universitaire de Sherbrooke, Sherbrooke, QC J1H5N4, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8977-3629","authenticated-orcid":false,"given":"Jean-Fran\u00e7ois","family":"Beaulieu","sequence":"additional","affiliation":[{"name":"Laboratory of Intestinal Physiopathology, Faculty of Medicine and Health Sciences, Universit\u00e9 de Shebrooke, Sherbrooke, QC J1H5N4, Canada"},{"name":"Centre de Recherche du Centre Hospitalier Universitaire de Sherbrooke, Sherbrooke, QC J1H5N4, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,3,31]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"e46589","DOI":"10.15252\/embr.201846589","article-title":"Mixed signals from the cell\u2019s antennae: Primary cilia in cancer","volume":"19","author":"Eguether","year":"2018","journal-title":"EMBO Rep."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1007\/s12079-021-00615-5","article-title":"Primary cilia: Turning points in establishing their ubiquity, sensory role and the pathological consequences of dysfunction","volume":"15","author":"Wheatley","year":"2021","journal-title":"J. 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