{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T04:37:18Z","timestamp":1787027838858,"version":"build-2736575974"},"reference-count":51,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2023,4,13]],"date-time":"2023-04-13T00:00:00Z","timestamp":1681344000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Research Foundation of Korea (NRF)","award":["NRF-2020R1A6A1A03043283"],"award-info":[{"award-number":["NRF-2020R1A6A1A03043283"]}]},{"name":"National Research Foundation of Korea (NRF)","award":["NRF-2021R1I1A3047407"],"award-info":[{"award-number":["NRF-2021R1I1A3047407"]}]},{"name":"National Research Foundation of Korea (NRF)","award":["NRF-2019M3D1A1078943]"],"award-info":[{"award-number":["NRF-2019M3D1A1078943]"]}]},{"name":"National Research Foundation of Korea (NRF)","award":["NRF-2020R1A6A1A03043283"],"award-info":[{"award-number":["NRF-2020R1A6A1A03043283"]}]},{"name":"National Research Foundation of Korea (NRF)","award":["NRF-2021R1I1A3047407"],"award-info":[{"award-number":["NRF-2021R1I1A3047407"]}]},{"name":"National Research Foundation of Korea (NRF)","award":["NRF-2019M3D1A1078943]"],"award-info":[{"award-number":["NRF-2019M3D1A1078943]"]}]},{"name":"Dankook University Hospital","award":["NRF-2020R1A6A1A03043283"],"award-info":[{"award-number":["NRF-2020R1A6A1A03043283"]}]},{"name":"Dankook University Hospital","award":["NRF-2021R1I1A3047407"],"award-info":[{"award-number":["NRF-2021R1I1A3047407"]}]},{"name":"Dankook University Hospital","award":["NRF-2019M3D1A1078943]"],"award-info":[{"award-number":["NRF-2019M3D1A1078943]"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells"],"abstract":"<jats:p>Diabetes mellitus contributes to 15\u201325% of all chronic foot ulcers. Peripheral vascular disease is a cause of ischemic ulcers and exacerbates diabetic foot disease. Cell-based therapies are viable options to restore damaged vessels and induce the formation of new vessels. Adipose-derived stem cells (ADSCs) have the potential for angiogenesis and regeneration because of their greater paracrine effect. Preclinical studies are currently using other forced enhancement techniques (e.g., genetic modification or biomaterials) to increase the efficacy of human ADSC (hADSC) autotransplantation. Unlike genetic modifications and biomaterials, many growth factors have been approved by the equivalent regulatory authorities. This study confirmed the effect of enhanced human ADSC (ehADSC)s with a cocktail of FGF and other pharmacological agents to promote wound healing in diabetic foot disease. In vitro, ehADSCs exhibited a long and slender spindle-shaped morphology and showed significantly increased proliferation. In addition, it was shown that ehADSCs have more functionalities in oxidative stress toleration, stem cell stemness, and mobility. In vivo, the local transplantation of 1.2 \u00d7 106 hADSCs or ehADSCs was performed in animals with diabetes induced by STZ. The ehADSC group showed a statistically decreased wound size and increased blood flow compared with the hADSC group and the sham group. Human Nucleus Antigen (HNA) positive cells were observed in some ADSC-transplanted animals. The ehADSC group showed a relatively higher portion of HNA-positive animals than the hADSC group. The blood glucose levels showed no significant difference among the groups. In conclusion, the ehADSCs showed a better performance in vitro, compared with conventional hADSCs. Additionally, a topical injection of ehADSCs into diabetic wounds enhanced wound healing and blood flow, while improving histological markers suggesting revascularization.<\/jats:p>","DOI":"10.3390\/cells12081146","type":"journal-article","created":{"date-parts":[[2023,4,13]],"date-time":"2023-04-13T01:35:00Z","timestamp":1681349700000},"page":"1146","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Human Fibroblast Growth Factor-Treated Adipose-Derived Stem Cells Facilitate Wound Healing and Revascularization in Rats with Streptozotocin-Induced Diabetes Mellitus"],"prefix":"10.3390","volume":"12","author":[{"given":"So-Young","family":"Chang","sequence":"first","affiliation":[{"name":"Beckman Laser Institute Korea, Dankook University, Cheonan 31116, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun Hee","family":"Lee","sequence":"additional","affiliation":[{"name":"Institute of Tissue Regeneration Engineering (ITREN), Dankook University, Cheonan 31116, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Se Cheol","family":"Oh","sequence":"additional","affiliation":[{"name":"Stem Cell R&D Center, N-BIOTEK Inc., Bucheon 14449, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6860-8042","authenticated-orcid":false,"given":"Min Young","family":"Lee","sequence":"additional","affiliation":[{"name":"Beckman Laser Institute Korea, Dankook University, Cheonan 31116, Republic of Korea"},{"name":"Department of Otolaryngology-Head & Neck Surgery, College of Medicine, Dankook University, Cheonan 31116, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0964-983X","authenticated-orcid":false,"given":"Nam Kyu","family":"Lim","sequence":"additional","affiliation":[{"name":"Department of Plastic and Reconstructive surgery, College of medicine, Dankook University, Cheonan 31116, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,4,13]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"388","DOI":"10.1046\/j.1365-2133.2003.05222.x","article-title":"Causes, investigation and treatment of leg ulceration","volume":"148","author":"Mekkes","year":"2003","journal-title":"Br. 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