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Given the similarity to human counterparts, the non-human primate (NHP) is an ideal preclinical model to evaluate several questions, including delivery, biodistribution, and immune response. While the generation of human-induced PS (iPS)-cell-derived myogenic progenitors is well established, there have been no data for NHP counterparts, probably due to the lack of an efficient system to differentiate NHP iPS cells towards the skeletal muscle lineage. Here, we report the generation of three independent Macaca fascicularis iPS cell lines and their myogenic differentiation using PAX7 conditional expression. The whole-transcriptome analysis confirmed the successful sequential induction of mesoderm, paraxial mesoderm, and myogenic lineages. NHP myogenic progenitors efficiently gave rise to myotubes under appropriate in vitro differentiation conditions and engrafted in vivo into the TA muscles of NSG and FKRP-NSG mice. Lastly, we explored the preclinical potential of these NHP myogenic progenitors in a single wild-type NHP recipient, demonstrating engraftment and characterizing the interaction with the host immune response. These studies establish an NHP model system through which iPS-cell-derived myogenic progenitors can be studied.<\/jats:p>","DOI":"10.3390\/cells12081147","type":"journal-article","created":{"date-parts":[[2023,4,13]],"date-time":"2023-04-13T01:35:00Z","timestamp":1681349700000},"page":"1147","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Establishment of Skeletal Myogenic Progenitors from Non-Human Primate Induced Pluripotent Stem Cells"],"prefix":"10.3390","volume":"12","author":[{"given":"June","family":"Baik","sequence":"first","affiliation":[{"name":"Department of Medicine, University of Minnesota, Minneapolis, MN 55455, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6953-0366","authenticated-orcid":false,"given":"Carolina","family":"Ortiz-Cordero","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of Minnesota, Minneapolis, MN 55455, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alessandro","family":"Magli","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of Minnesota, Minneapolis, MN 55455, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karim","family":"Azzag","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of Minnesota, Minneapolis, MN 55455, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah B.","family":"Crist","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of Minnesota, Minneapolis, MN 55455, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aline","family":"Yamashita","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of Minnesota, Minneapolis, MN 55455, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James","family":"Kiley","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of Minnesota, Minneapolis, MN 55455, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sridhar","family":"Selvaraj","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of Minnesota, Minneapolis, MN 55455, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ricardo","family":"Mondragon-Gonzalez","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of Minnesota, Minneapolis, MN 55455, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elizabeth","family":"Perrin","sequence":"additional","affiliation":[{"name":"Stem Cell Resources and the Wisconsin National Primate Research Center, University of Wisconsin, Madison, WI 53715, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John P.","family":"Maufort","sequence":"additional","affiliation":[{"name":"Stem Cell Resources and the Wisconsin National Primate Research Center, University of Wisconsin, Madison, WI 53715, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jody L.","family":"Janecek","sequence":"additional","affiliation":[{"name":"Department of Surgery, University of Minnesota, Minneapolis, MN 55455, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rachael M.","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Surgery, University of Minnesota, Minneapolis, MN 55455, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura Hocum","family":"Stone","sequence":"additional","affiliation":[{"name":"Department of Surgery, University of Minnesota, Minneapolis, MN 55455, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-5955-397X","authenticated-orcid":false,"given":"Parthasarathy","family":"Rangarajan","sequence":"additional","affiliation":[{"name":"Department of Surgery, University of Minnesota, Minneapolis, MN 55455, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2239-8083","authenticated-orcid":false,"given":"Sabarinathan","family":"Ramachandran","sequence":"additional","affiliation":[{"name":"Department of Surgery, University of Minnesota, Minneapolis, MN 55455, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2475-6975","authenticated-orcid":false,"given":"Melanie L.","family":"Graham","sequence":"additional","affiliation":[{"name":"Department of Surgery, University of Minnesota, Minneapolis, MN 55455, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rita C. R.","family":"Perlingeiro","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of Minnesota, Minneapolis, MN 55455, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,4,13]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"663","DOI":"10.1016\/j.cell.2006.07.024","article-title":"Induction of Pluripotent Stem Cells from Mouse Embryonic and Adult Fibroblast Cultures by Defined Factors","volume":"126","author":"Takahashi","year":"2006","journal-title":"Cell"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1038\/s41536-022-00249-0","article-title":"Transplantation of PSC-derived myogenic progenitors counteracts disease phenotypes in FSHD mice","volume":"7","author":"Azzag","year":"2022","journal-title":"NPJ Regen. Med."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1186\/s13395-020-00228-3","article-title":"Efficient engraftment of pluripotent stem cell-derived myogenic progenitors in a novel immunodeficient mouse model of limb girdle muscular dystrophy 2I","volume":"10","author":"Azzag","year":"2020","journal-title":"Skelet. 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Ther."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1089\/scd.2014.0374","article-title":"Nonhuman Primate Models in Translational Regenerative Medicine","volume":"23","author":"Daadi","year":"2014","journal-title":"Stem Cells Dev."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"352","DOI":"10.20411\/pai.v2i3.186","article-title":"The Critical Role of Nonhuman Primates in Medical Research","volume":"2","author":"Friedman","year":"2017","journal-title":"Pathog. Immun."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"5267","DOI":"10.1182\/bloodadvances.2022006984","article-title":"Assessment of safety and immunogenicity of MHC homozygous iPSC-derived CD34+ hematopoietic progenitors in an NHP model","volume":"6","author":"Kumar","year":"2022","journal-title":"Blood Adv."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"2392","DOI":"10.1016\/j.stemcr.2022.09.014","article-title":"Competency of iPSC-derived retinas in MHC-mismatched transplantation in non-human primates","volume":"17","author":"Uyama","year":"2022","journal-title":"Stem. Cell Rep."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1097\/00007890-198807000-00001","article-title":"Xenogeneic transplantation: A review","volume":"46","author":"Auchincloss","year":"1988","journal-title":"Transplantation"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"433","DOI":"10.1146\/annurev.immunol.16.1.433","article-title":"Xenogeneic transplantation","volume":"16","author":"Auchincloss","year":"1998","journal-title":"Annu. Rev. Immunol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1080\/08998280.2012.11928783","article-title":"A Brief History of Cross-Species Organ Transplantation","volume":"24","author":"Cooper","year":"2012","journal-title":"Bayl. Univ. Med. Cent. Proc."},{"key":"ref_15","first-page":"1","article-title":"Xenotransplantation: Past, present, and future directions","volume":"5","author":"Eissa","year":"2022","journal-title":"Highlights Biosci."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"2867","DOI":"10.1016\/j.celrep.2017.06.005","article-title":"PAX7 Targets, CD54, Integrin alpha9beta1, and SDC2, Allow Isolation of Human ESC\/iPSC-Derived Myogenic Progenitors","volume":"19","author":"Magli","year":"2017","journal-title":"Cell Rep."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"e47970","DOI":"10.7554\/eLife.47970","article-title":"Screening identifies small molecules that enhance the maturation of human pluripotent stem cell-derived myotubes","volume":"8","author":"Selvaraj","year":"2019","journal-title":"eLife"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"550","DOI":"10.1186\/s13059-014-0550-8","article-title":"Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2","volume":"15","author":"Love","year":"2014","journal-title":"Genome Biol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1038\/nprot.2008.211","article-title":"Systematic and integrative analysis of large gene lists using DAVID bioinformatics resources","volume":"4","author":"Huang","year":"2009","journal-title":"Nat. Protoc."},{"key":"ref_20","first-page":"100141","article-title":"clusterProfiler 4.0: A universal enrichment tool for interpreting omics data","volume":"2","author":"Wu","year":"2021","journal-title":"Innovation"},{"key":"ref_21","unstructured":"(2011). Guide for the Care and Use of Laboratory Animals, The National Academies Press. [8th ed.]."},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Palmer, S., Oppler, S.H., and Graham, M.L. (2022). Behavioral Management as a Coping Strategy for Managing Stressors in Primates: The Influence of Temperament and Species. Biology, 11.","DOI":"10.3390\/biology11030423"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1111\/j.1600-0684.2011.00525.x","article-title":"Successful implementation of cooperative handling eliminates the need for restraint in a complex non-human primate disease model","volume":"41","author":"Graham","year":"2012","journal-title":"J. Med. Primatol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"204","DOI":"10.1111\/j.1600-0684.2009.00340.x","article-title":"A novel alternative placement site and technique for totally implantable vascular access ports in non-human primates","volume":"38","author":"Graham","year":"2009","journal-title":"J. Med. Primatol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1080\/08941939.2018.1536178","article-title":"Long-Term Management of Vascular Access Ports in Nonhuman Primates Used in Preclinical Efficacy and Tolerability Studies","volume":"33","author":"Mutch","year":"2018","journal-title":"J. Investig. Surg."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1016\/j.devcel.2013.12.020","article-title":"Gene Regulatory Networks and Transcriptional Mechanisms that Control Myogenesis","volume":"28","author":"Buckingham","year":"2014","journal-title":"Dev. Cell"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"2104","DOI":"10.1242\/dev.151035","article-title":"Making muscle: Skeletal myogenesis in vivo and in vitro","volume":"144","author":"Chal","year":"2017","journal-title":"Development"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1573","DOI":"10.1016\/j.celrep.2017.01.040","article-title":"In Vivo Human Somitogenesis Guides Somite Development from hPSCs","volume":"18","author":"Xi","year":"2017","journal-title":"Cell Rep."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1016\/j.stem.2020.06.006","article-title":"A Human Skeletal Muscle Atlas Identifies the Trajectories of Stem and Progenitor Cells across Development and from Human Pluripotent Stem Cells","volume":"27","author":"Xi","year":"2020","journal-title":"Cell Stem Cell"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"671","DOI":"10.3727\/000000002783985378","article-title":"Dynamics of the Early Immune Cellular Reactions after Myogenic Cell Transplantation","volume":"11","author":"Skuk","year":"2002","journal-title":"Cell Transplant."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"13","DOI":"10.3727\/096368912X661337","article-title":"Intramuscular Transplantation of Myogenic Cells in Primates: Importance of Needle Size, Cell Number, and Injection Volume","volume":"23","author":"Skuk","year":"2014","journal-title":"Cell Transplant."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"620","DOI":"10.1016\/j.stemcr.2013.10.007","article-title":"Derivation and FACS-mediated purification of PAX3+\/PAX7+ skeletal muscle precursors from human pluripotent stem cells","volume":"1","author":"Borchin","year":"2013","journal-title":"Stem Cell Rep."},{"key":"ref_33","first-page":"46","article-title":"ERBB3 and NGFR mark a distinct skeletal muscle progenitor cell in human development and hPSCs","volume":"20","author":"Hicks","year":"2018","journal-title":"Nature"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1966","DOI":"10.1016\/j.celrep.2018.10.067","article-title":"A Myogenic Double-Reporter Human Pluripotent Stem Cell Line Allows Prospective Isolation of Skeletal Muscle Progenitors","volume":"25","author":"Wu","year":"2018","journal-title":"Cell Rep."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"883","DOI":"10.1016\/j.stemcr.2021.03.004","article-title":"Characterization of hiPSC-Derived Muscle Progenitors Reveals Distinctive Markers for Myogenic Cell Purification Toward Cell Therapy","volume":"16","author":"Nalbandian","year":"2021","journal-title":"Stem Cell Rep."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"2301","DOI":"10.1016\/j.celrep.2016.05.016","article-title":"Concordant but Varied Phenotypes among Duchenne Muscular Dystrophy Patient-Specific Myoblasts Derived using a Human iPSC-Based Model","volume":"15","author":"Choi","year":"2016","journal-title":"Cell Rep."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"4346","DOI":"10.1073\/pnas.1808303116","article-title":"Pluripotent stem cell-derived myogenic progenitors remodel their molecular signature upon in vivo engraftment","volume":"116","author":"Incitti","year":"2019","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"587","DOI":"10.1016\/j.stem.2008.10.014","article-title":"Generation of Induced Pluripotent Stem Cells from Adult Rhesus Monkey Fibroblasts","volume":"3","author":"Liu","year":"2008","journal-title":"Cell Stem Cell"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"312","DOI":"10.1016\/j.stemcr.2016.01.012","article-title":"Cardiomyocytes Derived from MHC-Homozygous Induced Pluripotent Stem Cells Exhibit Reduced Allogeneic Immunogenicity in MHC-Matched Non-human Primates","volume":"6","author":"Kawamura","year":"2016","journal-title":"Stem Cell Rep."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"385","DOI":"10.1038\/s41467-017-00926-5","article-title":"MHC matching improves engraftment of iPSC-derived neurons in non-human primates","volume":"8","author":"Morizane","year":"2017","journal-title":"Nat. Commun."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"94","DOI":"10.1016\/j.actbio.2022.05.012","article-title":"Articular cartilage reconstruction with TGF-beta1-simulating self-assembling peptide hydrogel-based composite scaffold","volume":"146","author":"Ye","year":"2022","journal-title":"Acta Biomater."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"1714","DOI":"10.1016\/j.stemcr.2022.05.018","article-title":"Long-term survival in non-human primates of stem cell-derived, MHC-unmatched corneal epithelial cell sheets","volume":"17","author":"Yoshinaga","year":"2022","journal-title":"Stem Cell Rep."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"770","DOI":"10.1097\/NEN.0b013e31822a6baa","article-title":"Transplanted Myoblasts Can Migrate Several Millimeters to Fuse with Damaged Myofibers in Nonhuman Primate Skeletal Muscle","volume":"70","author":"Skuk","year":"2011","journal-title":"J. Neuropathol. Exp. Neurol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"997","DOI":"10.1016\/j.nmd.2012.05.005","article-title":"Necrosis, sarcolemmal damage and apoptotic events in myofibers rejected by CD8+ lymphocytes: Observations in nonhuman primates","volume":"22","author":"Skuk","year":"2012","journal-title":"Neuromuscul. Disord."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"e228","DOI":"10.1097\/TXD.0000000000000740","article-title":"De Novo Circulating Antidonor\u2019s Cell Antibodies During Induced Acute Rejection of Allogeneic Myofibers in Myogenic Cell Transplantation: A Study in Nonhuman Primates","volume":"3","author":"Skuk","year":"2017","journal-title":"Transpl. Direct"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"232","DOI":"10.1016\/j.omtm.2017.05.002","article-title":"Cell Therapy in Myology: Dynamics of Muscle Precursor Cell Death after Intramuscular Administration in Non-human Primates","volume":"5","author":"Skuk","year":"2017","journal-title":"Mol. Ther-Methods Clin. Dev."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"574","DOI":"10.1038\/nature05282","article-title":"Mesoangioblast stem cells ameliorate muscle function in dystrophic dogs","volume":"444","author":"Sampaolesi","year":"2006","journal-title":"Nature"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1093\/jnen\/nly106","article-title":"Sarcolemmal Complement Membrane Attack Complex Deposits During Acute Rejection of Myofibers in Nonhuman Primates","volume":"78","author":"Skuk","year":"2019","journal-title":"J. Neuropathol. Exp. Neurol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"773","DOI":"10.1007\/s00251-010-0481-9","article-title":"Characterization of Mauritian cynomolgus macaque major histocompatibility complex class I haplotypes by high-resolution pyrosequencing","volume":"62","author":"Budde","year":"2010","journal-title":"Immunogenetics"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1111\/j.1600-0684.2008.00299.x","article-title":"MHC haplotype frequencies in a UK breeding colony of Mauritian cynomolgus macaques mirror those found in a distinct population from the same geographic origin","volume":"38","author":"Mee","year":"2009","journal-title":"J. Med. Primatol."},{"key":"ref_51","first-page":"444","article-title":"Renal transplantation in baboons under FK 506","volume":"106","author":"Todo","year":"1989","journal-title":"Surgery"}],"container-title":["Cells"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2073-4409\/12\/8\/1147\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T19:15:12Z","timestamp":1760123712000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2073-4409\/12\/8\/1147"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,4,13]]},"references-count":51,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2023,4]]}},"alternative-id":["cells12081147"],"URL":"https:\/\/doi.org\/10.3390\/cells12081147","relation":{},"ISSN":["2073-4409"],"issn-type":[{"value":"2073-4409","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,4,13]]}}}