{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,8]],"date-time":"2025-11-08T18:03:20Z","timestamp":1762625000519,"version":"build-2065373602"},"reference-count":43,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2023,4,23]],"date-time":"2023-04-23T00:00:00Z","timestamp":1682208000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004569","name":"Ministry of Science and Higher Education","doi-asserted-by":"publisher","award":["5160\/B\/P01\/2010\/39"],"award-info":[{"award-number":["5160\/B\/P01\/2010\/39"]}],"id":[{"id":"10.13039\/501100004569","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells"],"abstract":"<jats:p>The clinical outcome of sarcoidosis (SA) is very similar to tuberculosis (TB); however, they are treated differently and should not be confused. In search for their biomarkers, we have previously revealed changes in the phagocytic activity of monocytes in sarcoidosis and tuberculosis. On these monocytes we found a higher expression of receptors for the Fc fragment of immunoglobulin G (Fc\u03b3R) in SA and TB patients vs. healthy controls. Fc\u03b3Rs are responsible for the binding of immune complexes (ICs) to initiate an (auto)immune response and for ICs clearance. Surprisingly, our SA patients had a high blood level of ICs, despite the abundant presence of Fc\u03b3Rs. It pointed to Fc\u03b3R disfunction, presumably caused by the polymorphism of their (FCGR) genes. Therefore, we present here an analysis of the occurrence of FCGR2A, FCGR2B, FCGR2C, FCGR3A and FCGR3B variants in Caucasian SA and TB patients, and healthy individuals with the use of polymerase chain reaction (PCR) and real-time PCR. The presented data point to a possibility of supporting the differential diagnosis of SA and TB by analyzing FCGR2C, FCGR3A and FCGR3B polymorphism, while for severe stages of SA also by studying FCGR2A variants. Additionally, the genotyping of FCGR2A and FCGR3B might serve as a marker of SA progression.<\/jats:p>","DOI":"10.3390\/cells12091221","type":"journal-article","created":{"date-parts":[[2023,4,24]],"date-time":"2023-04-24T01:35:44Z","timestamp":1682300144000},"page":"1221","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Comparative Analysis of FCGR Gene Polymorphism in Pulmonary Sarcoidosis and Tuberculosis"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9687-1016","authenticated-orcid":false,"given":"Marlena","family":"Typiak","sequence":"first","affiliation":[{"name":"Department of General and Medical Biochemistry, Faculty of Biology, University of Gdansk, 80-308 Gdansk, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4290-3668","authenticated-orcid":false,"given":"Bart\u0142omiej","family":"R\u0119kawiecki","sequence":"additional","affiliation":[{"name":"Department of Pulmonology, Medical University of Gdansk, 80-214 Gdansk, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2236-7455","authenticated-orcid":false,"given":"Krzysztof","family":"R\u0119ba\u0142a","sequence":"additional","affiliation":[{"name":"Department of Forensic Medicine, Medical University of Gdansk, 80-204 Gdansk, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna","family":"Dubaniewicz","sequence":"additional","affiliation":[{"name":"Department of Pulmonology, Medical University of Gdansk, 80-214 Gdansk, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,4,23]]},"reference":[{"key":"ref_1","unstructured":"(1999). Statement on Sarcoidosis. Joint Statement of the American Thoracic Society (ATS), the European Respiratory Society (ERS) and the World Association of Sarcoidosis and Other Granulomatous Disorders (WASOG) adopted by the ATS Board of Directors and by the ERS Executive Committee, February 1999. Am. J. Respir. Crit. Care Med., 160, 736\u2013755."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"S\u00e8ve, P., Pacheco, Y., Durupt, F., Jamilloux, Y., Gerfaud-Valentin, M., Isaac, S., Boussel, L., Calender, A., Androdias, G., and Valeyre, D. (2021). Sarcoidosis: A Clinical Overview from Symptoms to Diagnosis. Cells, 10.","DOI":"10.3390\/cells10040766"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1007\/978-94-007-4549-0_29","article-title":"Sarcoidosis and tuberculosis: A connection to the human leukocyte antigen system","volume":"756","author":"Dubaniewicz","year":"2013","journal-title":"Adv. Exp. Med. Biol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1700991","DOI":"10.1183\/13993003.00991-2017","article-title":"Phenotypes of organ involvement in sarcoidosis","volume":"51","author":"Schupp","year":"2018","journal-title":"Eur. Respir. J."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1586\/eci.13.8","article-title":"Tuberculosis in the course of sarcoidosis treatment: Is genotyping necessary for personalized therapy?","volume":"9","author":"Dubaniewicz","year":"2013","journal-title":"Expert Rev. Clin. Immunol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1642","DOI":"10.1016\/S0140-6736(19)30308-3","article-title":"Tuberculosis","volume":"393","author":"Furin","year":"2019","journal-title":"Lancet"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"3448","DOI":"10.1128\/JCM.01433-06","article-title":"Mycobacterium tuberculosis complex and mycobacterial heat shock proteins in lymph node tissue from patients with pulmonary sarcoidosis","volume":"44","author":"Dubaniewicz","year":"2006","journal-title":"J. Clin. Microbiol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"419","DOI":"10.1016\/j.autrev.2009.11.015","article-title":"Mycobacterium tuberculosis heat shock proteins and autoimmunity in sarcoidosis","volume":"9","author":"Dubaniewicz","year":"2010","journal-title":"Autoimmun. Rev."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1550","DOI":"10.1016\/j.humimm.2013.08.275","article-title":"Microbial and human heat shock proteins as \u2019danger signals\u2019 in sarcoidosis","volume":"74","author":"Dubaniewicz","year":"2013","journal-title":"Hum. Immunol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1016\/j.humimm.2012.10.007","article-title":"Is mycobacterial heat shock protein 16 kDa, a marker of the dormant stage of Mycobacterium tuberculosis, a sarcoid antigen?","volume":"74","author":"Dubaniewicz","year":"2013","journal-title":"Hum. Immunol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"788","DOI":"10.1016\/j.humimm.2012.05.005","article-title":"Changed phagocytic activity and pattern of Fc\u03b3 and complement receptors on blood monocytes in sarcoidosis","volume":"73","author":"Dubaniewicz","year":"2012","journal-title":"Hum. Immunol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1007\/5584_2015_193","article-title":"Polymorphism of FCGR2A, FCGR2C, and FCGR3B Genes in the Pathogenesis of Sarcoidosis","volume":"905","author":"Typiak","year":"2016","journal-title":"Adv. Exp. Med. Biol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1016\/j.humimm.2014.02.011","article-title":"Polymorphism of FCGR3A gene in sarcoidosis","volume":"75","author":"Typiak","year":"2014","journal-title":"Hum. Immunol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1038\/nri2206","article-title":"Fcgamma receptors as regulators of immune responses","volume":"8","author":"Nimmerjahn","year":"2008","journal-title":"Nat. Rev. Immunol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"2153","DOI":"10.1056\/NEJMra071714","article-title":"Sarcoidosis","volume":"357","author":"Iannuzzi","year":"2007","journal-title":"N. Engl. J. Med."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"5444","DOI":"10.1093\/nar\/19.19.5444","article-title":"A rapid non-enzymatic method for the preparation of HMW DNA from blood for RFLP studies","volume":"19","author":"Lahiri","year":"1991","journal-title":"Nucleic Acids Res."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"2132","DOI":"10.1002\/art.10438","article-title":"Genetic linkage and association of Fcgamma receptor IIIA (CD16A) on chromosome 1q23 with human systemic lupus erythematosus","volume":"46","author":"Edberg","year":"2002","journal-title":"Arthritis Rheum."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"S51","DOI":"10.1038\/sj.gene.6363879","article-title":"Genomic organization of classical human low-affinity Fcgamma receptor genes","volume":"3","author":"Su","year":"2002","journal-title":"Genes Immun."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"374","DOI":"10.1034\/j.1399-0039.2003.00047.x","article-title":"Association of Fcgamma receptor IIb and IIIb polymorphisms with susceptibility to systemic lupus erythematosus in Thais","volume":"61","author":"Siriboonrit","year":"2003","journal-title":"Tissue Antigens"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1046\/j.1365-2567.2001.01284.x","article-title":"Fcgamma receptor polymorphisms in populations in Ethiopia and Norway","volume":"104","author":"Myhr","year":"2001","journal-title":"Immunology"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"790","DOI":"10.1086\/302809","article-title":"Minor lesion mutational spectrum of the entire NF1 gene does not explain its high mutability but points to a functional domain upstream of the GAP-related domain","volume":"66","author":"Fahsold","year":"2000","journal-title":"Am. J. Hum. Genet."},{"key":"ref_22","unstructured":"Lowry, R. (2022). Vassar Stats Program, Vassar College. Available online: http:\/\/www.vassarstats.net\/."},{"key":"ref_23","unstructured":"OEGE Software Organization (2021, December 15). Odds Ratio Calculator. Available online: www.oege.org\/software\/orcalc.html."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"505","DOI":"10.1093\/aje\/kwn359","article-title":"Hardy-Weinberg equilibrium testing of biological ascertainment for Mendelian randomization studies","volume":"169","author":"Rodriguez","year":"2009","journal-title":"Am. J. Epidemiol."},{"key":"ref_25","first-page":"P2635","article-title":"Phagocytosis by blood monocytes in differentiation between sarcoidosis and tuberculosis","volume":"40","author":"Dubaniewicz","year":"2012","journal-title":"Eur. Respir. J."},{"key":"ref_26","first-page":"PA1378","article-title":"Monocyte\/neutrophil phagocytic activity ratio in differentiating tuberculosis from sarcoidosis","volume":"54","author":"Dubaniewicz","year":"2019","journal-title":"Eur. Respir. J."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1007\/s10875-006-9011-9","article-title":"Comparative analysis of mycobacterial heat shock proteins-induced apoptosis of peripheral blood mononuclear cells in sarcoidosis and tuberculosis","volume":"26","author":"Dubaniewicz","year":"2006","journal-title":"J. Clin. Immunol."},{"key":"ref_28","first-page":"108","article-title":"Frequency of occurrence of circulating immune complexes in sera of patients with pulmonary sarcoidosis; preliminary report","volume":"3","author":"Dubaniewicz","year":"2004","journal-title":"Vestnik MCDC"},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Dubaniewicz, A. (2023). Mycobacterial Heat Shock Proteins in Sarcoidosis and Tuberculosis. Int. J. Mol. Sci., 24.","DOI":"10.3390\/ijms24065084"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1016\/j.it.2015.04.005","article-title":"Fc\u03b3R dependent mechanisms of cytotoxic, agonistic, and neutralizing antibody activities","volume":"36","author":"Nimmerjahn","year":"2015","journal-title":"Trends Immunol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1016\/S1673-8527(09)60044-8","article-title":"Analysis of MIF, FCGR2A and FCGR3A gene polymorphisms with susceptibility to pulmonary tuberculosis in Moroccan population","volume":"37","author":"Sadki","year":"2010","journal-title":"J. Genet. Genom."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"508","DOI":"10.3389\/fimmu.2015.00508","article-title":"Mechanisms of Control of Mycobacterium tuberculosis by NK Cells: Role of Glutathione","volume":"6","author":"Allen","year":"2015","journal-title":"Front. Immunol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"240","DOI":"10.1177\/1753425919881176","article-title":"Role of neutrophils in tuberculosis: A bird\u2019s eye view","volume":"26","author":"Hilda","year":"2020","journal-title":"Innate Immun."},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Typiak, M., R\u0119ba\u0142a, K., Hara\u015b, A., Skotarczak, M., S\u0142omi\u0144ski, J.M., and Dubaniewicz, A. (2017). Copy number variation of FCGR genes in etiopathogenesis of sarcoidosis. PLoS ONE, 12.","DOI":"10.1371\/journal.pone.0177194"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"244","DOI":"10.1111\/j.1365-2249.2009.03980.x","article-title":"Functional and clinical consequences of Fc receptor polymorphic and copy number variants","volume":"157","author":"Bournazos","year":"2009","journal-title":"Clin. Exp. Immunol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"380","DOI":"10.1038\/gene.2009.35","article-title":"Fcgamma receptors: Structure, function and role as genetic risk factors in SLE","volume":"10","author":"Li","year":"2009","journal-title":"Genes Immun."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1002\/path.1710980105","article-title":"The production of granulomata by antigen-antibody complexes","volume":"98","author":"Spector","year":"1969","journal-title":"J. Pathol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"311","DOI":"10.1038\/nrd2909","article-title":"Fc receptor-targeted therapies for the treatment of inflammation, cancer and beyond","volume":"11","author":"Hogarth","year":"2012","journal-title":"Nat. Rev. Drug. Discov."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s10875-005-0353-8","article-title":"Fc receptors and their role in immune regulation and autoimmunity","volume":"25","author":"Takai","year":"2005","journal-title":"J. Clin. Immunol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"236","DOI":"10.1111\/imr.12335","article-title":"The biology of the classical Fc\u03b3 receptors in non-hematopoietic cells","volume":"268","author":"Anderson","year":"2015","journal-title":"Immunol. Rev."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"35","DOI":"10.4049\/jimmunol.1302232","article-title":"Cutting edge: Expression of Fc\u03b3RIIB tempers memory CD8 T cell function in vivo","volume":"192","author":"Badovinac","year":"2014","journal-title":"J. Immunol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"1029","DOI":"10.1182\/blood-2007-03-079913","article-title":"Copy number variation of the activating FCGR2C gene predisposes to idiopathic thrombocytopenic purpura","volume":"111","author":"Breunis","year":"2008","journal-title":"Blood"},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Adu, B., Dodoo, D., Adukpo, S., Hedley, P.L., Arthur, F.K., Gerds, T.A., Larsen, S.O., Christiansen, M., and Theisen, M. (2012). Fc \u03b3 receptor IIIB (Fc\u03b3RIIIB) polymorphisms are associated with clinical malaria in Ghanaian children. PLoS ONE, 7.","DOI":"10.1371\/journal.pone.0046197"}],"container-title":["Cells"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2073-4409\/12\/9\/1221\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T19:21:46Z","timestamp":1760124106000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2073-4409\/12\/9\/1221"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,4,23]]},"references-count":43,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2023,5]]}},"alternative-id":["cells12091221"],"URL":"https:\/\/doi.org\/10.3390\/cells12091221","relation":{},"ISSN":["2073-4409"],"issn-type":[{"type":"electronic","value":"2073-4409"}],"subject":[],"published":{"date-parts":[[2023,4,23]]}}}