{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T22:40:37Z","timestamp":1790376037816,"version":"4.1.0"},"reference-count":50,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2023,6,9]],"date-time":"2023-06-09T00:00:00Z","timestamp":1686268800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100010621","name":"Medical University of Lublin, Poland","doi-asserted-by":"publisher","award":["DS 474"],"award-info":[{"award-number":["DS 474"]}],"id":[{"id":"10.13039\/501100010621","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells"],"abstract":"<jats:p>(1) The treatment of metastatic or drug-resistant melanoma is still a significant therapeutic problem. The aim of this study was to evaluate the anticancer potential of daphnetin (7,8-dihydroxycoumarin) and its combinations with five different cytostatic drugs (mitoxantrone, docetaxel, vemurafenib, epirubicin and cisplatin). (2) The viability, proliferation and cytotoxicity of daphnetin against four human malignant melanoma cell lines were evaluated. The interactions were assessed using isobolographic analysis for the combinations of daphnetin with each of the five cytostatic drugs. (3) Daphnetin showed anticancer activity against malignant melanoma, with IC50 values ranging from 40.48 \u00b1 10.90 \u00b5M to 183.97 \u00b1 18.82 \u00b5M, depending on the cell line. The combination of daphnetin with either vemurafenib or epirubicin showed an antagonistic interaction. Moreover, additive interactions were observed for the combinations of daphnetin with cisplatin and docetaxel. The most desirable synergistic interactions for human melanoma metastatic cell lines were observed for the combination of daphnetin with mitoxantrone. (4) The obtained results suggest that daphnetin should not be combined with vemurafenib or epirubicin in the treatment of malignant melanoma due to the abolition of their anticancer effects. The combination of daphnetin with mitoxantrone is beneficial in the treatment of metastatic melanoma due to their synergistic interaction.<\/jats:p>","DOI":"10.3390\/cells12121593","type":"journal-article","created":{"date-parts":[[2023,6,9]],"date-time":"2023-06-09T01:32:34Z","timestamp":1686274354000},"page":"1593","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Daphnetin, a Coumarin with Anticancer Potential against Human Melanoma: In Vitro Study of Its Effective Combination with Selected Cytostatic Drugs"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7099-1968","authenticated-orcid":false,"given":"Paula","family":"Wr\u00f3blewska-\u0141uczka","sequence":"first","affiliation":[{"name":"Department of Occupational Medicine, Medical University of Lublin, ul. Jaczewskiego 8b, 20-090 Lublin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7075-1051","authenticated-orcid":false,"given":"Agnieszka","family":"G\u00f3ralczyk","sequence":"additional","affiliation":[{"name":"Department of Occupational Medicine, Medical University of Lublin, ul. Jaczewskiego 8b, 20-090 Lublin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3059-0393","authenticated-orcid":false,"given":"Jarogniew J.","family":"\u0141uszczki","sequence":"additional","affiliation":[{"name":"Department of Occupational Medicine, Medical University of Lublin, ul. 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Cancers, 14.","DOI":"10.3390\/cancers14205123"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"543","DOI":"10.1016\/j.cps.2021.05.001","article-title":"Melanoma Risk Factors and Prevention","volume":"48","author":"Dzwierzynski","year":"2021","journal-title":"Clin. Plast. Surg."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Lopes, J., Rodrigues, C.M.P., Gaspar, M.M., and Reis, C.P. (2022). Melanoma Management: From Epidemiology to Treatment and Latest Advances. Cancers, 14.","DOI":"10.3390\/cancers14194652"},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Villani, A., Potestio, L., Fabbrocini, G., Troncone, G., Malapelle, U., and Scalvenzi, M. (2022). The Treatment of Advanced Melanoma: Therapeutic Update. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms23126388"},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Fu, Z., Zhang, L., Hang, S., Wang, S., Li, N., Sun, X., Wang, Z., Sheng, R., Wang, F., and Wu, W. (2022). Synthesis of Coumarin Derivatives: A New Class of Coumarin-Based G Protein-Coupled Receptor Activators and Inhibitors. Polymers, 14.","DOI":"10.3390\/polym14102021"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"e2022002612022","DOI":"10.1002\/cbdv.202200261","article-title":"Daphnetin, a Coumarin in Genus Stellera Chamaejasme Linn: Chemistry, Bioactivity and Therapeutic Potential","volume":"19","author":"Hang","year":"2022","journal-title":"Chem. Biodivers."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1325","DOI":"10.2174\/092986710790936284","article-title":"Coumarins: Old compounds with novel promising therapeutic perspectives","volume":"17","author":"Riveiro","year":"2010","journal-title":"Curr. Med. Chem."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"993562","DOI":"10.3389\/fphar.2022.993562","article-title":"Daphnetin: A bioactive natural coumarin with diverse therapeutic potentials","volume":"13","author":"Javed","year":"2022","journal-title":"Front. Pharmacol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1016\/j.jphs.2018.11.013","article-title":"Daphnetin inhibits high glucose-induced extracellular matrix accumulation, oxidative stress and inflammation in human glomerular mesangial cells","volume":"139","author":"Xu","year":"2019","journal-title":"J. Pharmacol. Sci."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1220","DOI":"10.1007\/s43440-021-00261-z","article-title":"Mechanistic interplay of various mediators involved in mediating the neuroprotective effect of daphnetin","volume":"73","author":"Singh","year":"2021","journal-title":"Pharmacol. Rep."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1016\/j.phymed.2016.11.023","article-title":"7,8-Dihydroxycoumarin (daphnetin) protects INS-1 pancreatic \u03b2-cells against streptozotocin-induced apoptosis","volume":"24","author":"Vinayagam","year":"2017","journal-title":"Phytomedicine"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"605","DOI":"10.1080\/10799893.2020.1772298","article-title":"Virtual screening, ADME\/T, and binding free energy analysis of anti-viral, anti-protease, and anti-infectious compounds against NSP10\/NSP16 methyltransferase and main protease of SARS CoV-2","volume":"40","author":"Maurya","year":"2020","journal-title":"J. Recept. Signal Transduct. Res."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"2185","DOI":"10.1039\/D0RA06677J","article-title":"Quantitative proteomics approach to investigate the antibacterial response of Helicobacter pylori to daphnetin, a traditional Chinese medicine monomer","volume":"11","author":"Lu","year":"2021","journal-title":"RSC Adv."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"884548","DOI":"10.3389\/fphar.2022.884548","article-title":"Repositioning of Anti-Inflammatory Drugs for the Treatment of Cervical Cancer Sub-Types","volume":"13","author":"Kori","year":"2022","journal-title":"Front. Pharmacol."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Wr\u00f3blewska-\u0141uczka, P., Grabarska, A., Florek-\u0141uszczki, M., Plewa, Z., and \u0141uszczki, J.J. (2021). Synergy, Additivity, and Antagonism between Cisplatin and Selected Coumarins in Human Melanoma Cells. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms22020537"},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Wr\u00f3blewska-\u0141uczka, P., Cabaj, J., B\u0105k, W., Bargie\u0142, J., Grabarska, A., G\u00f3ralczyk, A., and \u0141uszczki, J.J. (2022). Additive Interactions between Betulinic Acid and Two Taxanes in In Vitro Tests against Four Human Malignant Melanoma Cell Lines. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms23179641"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"108274","DOI":"10.1016\/j.dib.2022.108274","article-title":"Impact of p53 knockdown on protein dataset of HaCaT cells","volume":"42","author":"Romashin","year":"2022","journal-title":"Data Brief."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Marz\u0119da, P., Wr\u00f3blewska-\u0141uczka, P., Drozd, M., Florek-\u0141uszczki, M., Za\u0142uska-Ogryzek, K., and \u0141uszczki, J.J. (2022). Cannabidiol Interacts Antagonistically with Cisplatin and Additively with Mitoxantrone in Various Melanoma Cell Lines\u2014An Isobolographic Analysis. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms23126752"},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Wr\u00f3blewska-\u0141uczka, P., Grabarska, A., G\u00f3ralczyk, A., Marz\u0119da, P., and \u0141uszczki, J.J. (2023). Fraxetin Interacts Additively with Cisplatin and Mitoxantrone, Antagonistically with Docetaxel in Various Human Melanoma Cell Lines\u2014An Isobolographic Analysis. Int. J. Mol. Sci., 24.","DOI":"10.3390\/ijms24010212"},{"key":"ref_21","first-page":"99","article-title":"A Simplified Method of Evaluating Dose-Effect Experiments","volume":"96","author":"Litchfield","year":"1949","journal-title":"J. Pharmacol. Exp. Ther."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1007\/s00210-007-0144-z","article-title":"Isobolographic analysis of interaction between drugs with nonparallel dose-response relationship curves: A practical application","volume":"375","author":"Luszczki","year":"2007","journal-title":"NaunynSchmiedebergs Arch. Pharmacol."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Grabarska, A., Wr\u00f3blewska-\u0141uczka, P., Kukula-Koch, W., \u0141uszczki, J.J., Kalpoutzakis, E., Adamczuk, G., Skaltsounis, A.L., and Stepulak, A. (2021). Palmatine, a Bioactive Protoberberine Alkaloid Isolated from Berberis cretica, Inhibits the Growth of Human Estrogen Receptor-Positive Breast Cancer Cells and Acts Synergistically and Additively with Doxorubicin. Molecules, 26.","DOI":"10.3390\/molecules26206253"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"947","DOI":"10.1016\/0024-3205(89)90148-3","article-title":"Statistical Analysis of Drug-Drug and Site-Site Interactions with Isobolograms","volume":"45","author":"Tallarida","year":"1989","journal-title":"Life Sci."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"981","DOI":"10.1124\/jpet.104.067264","article-title":"Isobolographic analysis for combinations of a full and partial agonist: Curved isoboles","volume":"310","author":"Grabovsky","year":"2004","journal-title":"J. Pharmacol. Exp. Ther."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1007\/978-1-4419-6306-2_17","article-title":"Combination analysis","volume":"678","author":"Tallarida","year":"2010","journal-title":"Adv. Exp. Med. Biol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"9.19.1","DOI":"10.1002\/0471141755.ph0919s72","article-title":"Drug Combinations: Tests and Analysis with Isoboles","volume":"72","author":"Tallarida","year":"2016","journal-title":"Curr. Protoc. Pharmacol."},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Krasowska, D., Gerkowicz, A., Wr\u00f3blewska-\u0141uczka, P., Grabarska, A., Za\u0142uska-Ogryzek, K., Krasowska, D., and \u0141uszczki, J.J. (2022). Anticancer Activity of Amantadine and Evaluation of Its Interactions with Selected Cytostatics in Relation to Human Melanoma Cells. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms23147653"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"2059","DOI":"10.1080\/10428190802353591","article-title":"Preclinical versus Clinical Drug Combination Studies","volume":"49","author":"Chou","year":"2008","journal-title":"Leuk. Lymphoma"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1003","DOI":"10.1177\/1947601912440575","article-title":"Quantitative methods for assessing drug synergism","volume":"2","author":"Tallarida","year":"2011","journal-title":"Genes Cancer"},{"key":"ref_31","first-page":"465","article-title":"Analysis of Cell Viability by the Lactate Dehydrogenase Assay","volume":"6","author":"Kumar","year":"2018","journal-title":"Cold Spring Harb. Protoc."},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Wr\u00f3blewska-\u0141uczka, P., G\u00f3ralczyk, A., and \u0141uszczki, J.J. (2023). Synergy, Additivity and Antagonism between Esculetin and Six Commonly Used Chemotherapeutics in Various Malignant Melanoma Cell Lines\u2014An Isobolographic Analysis. Molecules, 28.","DOI":"10.3390\/molecules28093889"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1016\/j.bbrc.2016.01.179","article-title":"Daphnetin inhibits invasion and migration of LM8 murine osteosarcoma cells by decreasing RhoA and Cdc42 expression","volume":"471","author":"Fukuda","year":"2016","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"682","DOI":"10.1006\/bbrc.1999.0958","article-title":"Daphnetin, one of coumarin derivatives, is a protein kinase inhibitor","volume":"260","author":"Yang","year":"1999","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"680611","DOI":"10.3389\/fimmu.2021.680611","article-title":"Screening for Active Compounds Targeting Human Natural Killer Cell Activation Identifying Daphnetin as an Enhancer for IFN-\u03b3 Production and Direct Cytotoxicity","volume":"12","author":"Yao","year":"2021","journal-title":"Front. Immunol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"939","DOI":"10.1111\/1440-1681.12608","article-title":"Daphnetin inhibits TNF-\u03b1 and VEGF-induced angiogenesis through inhibition of the IKKs\/I\u03baB\u03b1\/NF-\u03baB, Src\/FAK\/ERK1\/2 and Akt signalling pathways","volume":"43","author":"Kumar","year":"2016","journal-title":"Clin. Exp. Pharmacol. Physiol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"153465","DOI":"10.1016\/j.phymed.2021.153465","article-title":"Daphnetin triggers ROS-induced cell death and induces cytoprotective autophagy by modulating the AMPK\/Akt\/mTOR pathway in ovarian cancer","volume":"82","author":"Fan","year":"2021","journal-title":"Phytomedicine"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"952","DOI":"10.1002\/ddr.21920","article-title":"Daphnetin inhibits the survival of hepatocellular carcinoma cells through regulating Wnt\/\u03b2-catenin signaling pathway","volume":"83","author":"Liu","year":"2022","journal-title":"Drug Dev. Res."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"1779","DOI":"10.1016\/j.bcp.2004.01.014","article-title":"Daphnetin induced differentiation of human renal carcinoma cells and its mediation by p38 mitogen-activated protein kinase","volume":"67","author":"Finn","year":"2004","journal-title":"Biochem. Pharmacol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"575","DOI":"10.5650\/jos.ess21415","article-title":"Daphnetin Ameliorates the Expansion of Chemically Induced Hepatocellular Carcinoma via Reduction of Inflammation and Oxidative Stress","volume":"71","author":"Li","year":"2022","journal-title":"J. Oleo Sci."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1002\/jbt.22759","article-title":"Daphnetin exerts an anticancer effect by attenuating the pro-inflammatory cytokines","volume":"35","author":"Pei","year":"2021","journal-title":"J. Biochem. Mol. Toxicol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"115333","DOI":"10.1016\/j.taap.2020.115333","article-title":"Daphnetin ameliorates glucocorticoid-induced osteoporosis via activation of Wnt\/GSK-3\u03b2\/\u03b2-catenin signaling","volume":"409","author":"Wang","year":"2020","journal-title":"Toxicol. Appl. Pharmacol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"439","DOI":"10.1016\/j.biopha.2016.05.028","article-title":"Daphnetin ameliorates 7,12-dimethylbenz[a]anthracene-induced mammary carcinogenesis through Nrf-2-Keap1 and NF-\u03baB pathways","volume":"82","author":"Kumar","year":"2016","journal-title":"Biomed. Pharmacother."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1007\/s13105-018-0608-2","article-title":"7,8-Dihydroxycoumarin exerts antitumor potential on DMBA-induced mammary carcinogenesis by inhibiting ER\u03b1, PR, EGFR, and IGF1R: Involvement of MAPK1\/2-JNK1\/2-Akt pathway","volume":"74","author":"Kumar","year":"2018","journal-title":"J. Physiol. Biochem."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"596","DOI":"10.1093\/bbb\/zbac016","article-title":"Daphnetin inhibits \u03b1-MSH-induced melanogenesis via PKA and ERK signaling pathways in B16F10 melanoma cells","volume":"86","author":"Nam","year":"2022","journal-title":"Biosci. Biotechnol. Biochem."},{"key":"ref_46","first-page":"765","article-title":"In vitro anti-proliferative effect and in vivo antitumor action of daphnetin in different tumor cells","volume":"88","year":"2020","journal-title":"Cir. Cir."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"3489","DOI":"10.1158\/1078-0432.CCR-16-3083","article-title":"Clinically Relevant Concentrations of Anticancer Drugs: A Guide for Nonclinical Studies","volume":"23","author":"Liston","year":"2017","journal-title":"Clin. Cancer Res."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"579178","DOI":"10.3389\/fphar.2020.579178","article-title":"Daphnetin Attenuated Cisplatin-Induced Acute Nephrotoxicity With Enhancing Antitumor Activity of Cisplatin by Upregulating SIRT1\/SIRT6-Nrf2 Pathway","volume":"11","author":"Fan","year":"2020","journal-title":"Front. Pharmacol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1134\/S1607672921040128","article-title":"Chemoprotective effect of daphnetin in doxorubicin treated esophageal cancer stem cell xenograft tumor mouse","volume":"499","author":"Deng","year":"2021","journal-title":"Dokl. Biochem. Biophys."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1038\/nrd1984","article-title":"Targeting multidrug resistance in cancer","volume":"5","author":"Paterson","year":"2006","journal-title":"Nat. Rev. Drug. 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