{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T13:34:57Z","timestamp":1791293697827,"version":"4.1.0"},"reference-count":42,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2023,6,15]],"date-time":"2023-06-15T00:00:00Z","timestamp":1686787200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Science Centre, Poland","award":["UMO-2019\/35\/B\/NZ5\/00694"],"award-info":[{"award-number":["UMO-2019\/35\/B\/NZ5\/00694"]}]},{"name":"National Science Centre, Poland","award":["1WU\/1\/M\/MB\/N\/20\/20"],"award-info":[{"award-number":["1WU\/1\/M\/MB\/N\/20\/20"]}]},{"name":"Medical University of Warsaw","award":["UMO-2019\/35\/B\/NZ5\/00694"],"award-info":[{"award-number":["UMO-2019\/35\/B\/NZ5\/00694"]}]},{"name":"Medical University of Warsaw","award":["1WU\/1\/M\/MB\/N\/20\/20"],"award-info":[{"award-number":["1WU\/1\/M\/MB\/N\/20\/20"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells"],"abstract":"<jats:p>Different eosinophil subpopulations have been identified in asthma and other eosinophilic disorders. However, there is a paucity of data on eosinophil subpopulations in patients with chronic obstructive pulmonary disease (COPD). The aim of this study was to compare eosinophil phenotypes in blood and induced sputum in patients with COPD, asthma and controls. Stable patients with mild-to-moderate COPD (n = 15) and asthma (n = 14) with documented blood eosinophilia \u2265100 cells\/\u00b5L in the year prior to the study and the control group (n = 11) were included to the study. The blood and sputum eosinophil phenotypes were analyzed by flow cytometry. IL-5, IL-13, CCL5 and eotaxin-3 levels were measured in the induced sputum. The marker expression on blood eosinophils was similar among control, asthma and COPD groups. The expressions of CD125, CD193, CD14 and CD62L were higher on blood than on sputum eosinophils in all three groups. We found increased levels of CD193+ and CD66b+ sputum eosinophils from COPD patients, and an elevated level of CD11b+ sputum eosinophils in asthma compared to COPD patients. The results of our study suggest that the profile of marker expression on COPD sputum eosinophils differed from other groups, suggesting a distinct phenotype of eosinophils of COPD patients than in asthma or healthy subjects.<\/jats:p>","DOI":"10.3390\/cells12121631","type":"journal-article","created":{"date-parts":[[2023,6,15]],"date-time":"2023-06-15T01:32:57Z","timestamp":1686792777000},"page":"1631","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Blood and Sputum Eosinophils of COPD Patients Are Differently Polarized than in Asthma"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3953-3715","authenticated-orcid":false,"given":"Katarzyna","family":"Mycroft","sequence":"first","affiliation":[{"name":"Department of Internal Medicine, Pulmonary Diseases and Allergy, Medical University of Warsaw, 02-097 Warsaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0576-7289","authenticated-orcid":false,"given":"Magdalena","family":"Papli\u0144ska-Goryca","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Pulmonary Diseases and Allergy, Medical University of Warsaw, 02-097 Warsaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8835-3764","authenticated-orcid":false,"given":"Ma\u0142gorzata","family":"Proboszcz","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Pulmonary Diseases and Allergy, Medical University of Warsaw, 02-097 Warsaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2101-5397","authenticated-orcid":false,"given":"Patrycja","family":"Nejman-Gryz","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Pulmonary Diseases and Allergy, Medical University of Warsaw, 02-097 Warsaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0087-2031","authenticated-orcid":false,"given":"Rafa\u0142","family":"Krenke","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Pulmonary Diseases and Allergy, Medical University of Warsaw, 02-097 Warsaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4686-910X","authenticated-orcid":false,"given":"Katarzyna","family":"G\u00f3rska","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Pulmonary Diseases and Allergy, Medical University of Warsaw, 02-097 Warsaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,6,15]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2565","DOI":"10.1016\/j.jaip.2020.03.017","article-title":"Eosinophils in COPD\u2014Current Concepts and Clinical Implications","volume":"8","author":"Mycroft","year":"2020","journal-title":"J. Allergy Clin. Immunol. Pract."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Salvo-Romero, E., Rodi\u00f1o-Janeiro, B.K., Albert-Bayo, M., Lobo, B., Santos, J., Farr\u00e9, R., Martinez, C., and Vicario, M. (2022). Eosinophils in the Gastrointestinal Tract: Key Contributors to Neuro-Immune Crosstalk and Potential Implications in Disorders of Brain-Gut Interaction. Cells, 11.","DOI":"10.3390\/cells11101644"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1016\/j.crimmu.2022.03.002","article-title":"The Emerging Roles of Eosinophils: Implications for the Targeted Treatment of Eosinophilic-Associated Inflammatory Conditions","volume":"3","author":"Lombardi","year":"2022","journal-title":"Curr. Res. Immunol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"93","DOI":"10.3389\/fmed.2017.00093","article-title":"The Biology of Eosinophils and Their Role in Asthma","volume":"4","author":"McBrien","year":"2017","journal-title":"Front. Med."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"210150","DOI":"10.1183\/16000617.0150-2021","article-title":"Eosinophils and Eosinophilic Immune Dysfunction in Health and Disease","volume":"31","author":"Jackson","year":"2022","journal-title":"Eur. Respir. Rev."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"3896","DOI":"10.1182\/blood-2014-10-607788","article-title":"Eosinophil-Specific Deletion of I\u03baB\u03b1 in Mice Reveals a Critical Role of NF-\u039aB-Induced Bcl-XL for Inhibition of Apoptosis","volume":"125","author":"Schwartz","year":"2015","journal-title":"Blood"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1067\/mai.2003.3","article-title":"Receptor Internalization Is Required for Eotaxin-Induced Responses in Human Eosinophils","volume":"111","author":"Zimmermann","year":"2003","journal-title":"J. Allergy Clin. Immunol."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Whetstone, C.E., Ranjbar, M., Omer, H., Cusack, R.P., and Gauvreau, G.M. (2022). The Role of Airway Epithelial Cell Alarmins in Asthma. Cells, 11.","DOI":"10.3390\/cells11071105"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"354","DOI":"10.1080\/15412555.2019.1675150","article-title":"Relationship between Blood and Induced Sputum Eosinophils, Bronchial Hyperresponsiveness and Reversibility of Airway Obstruction in Mild-to-Moderate Chronic Obstructive Pulmonary Disease","volume":"16","author":"Proboszcz","year":"2019","journal-title":"COPD"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1671","DOI":"10.1056\/NEJMoa1713901","article-title":"Once-Daily Single-Inhaler Triple versus Dual Therapy in Patients with COPD","volume":"378","author":"Lipson","year":"2018","journal-title":"N. Engl. J. Med."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1016\/S2213-2600(18)30006-7","article-title":"Predictors of Exacerbation Risk and Response to Budesonide in Patients with Chronic Obstructive Pulmonary Disease: A Post-Hoc Analysis of Three Randomised Trials","volume":"6","author":"Bafadhel","year":"2018","journal-title":"Lancet Respir. Med."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"523","DOI":"10.1164\/rccm.201502-0235LE","article-title":"Blood Eosinophils: A Biomarker of Response to Extrafine Beclomethasone\/Formoterol in Chronic Obstructive Pulmonary Disease","volume":"192","author":"Siddiqui","year":"2015","journal-title":"Am. J. Respir. Crit. Care Med."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"302","DOI":"10.1016\/j.amjmed.2021.09.006","article-title":"The Use of Inhaled Corticosteroids for Patients with COPD Who Continue to Smoke Cigarettes: An Evaluation of Current Practice","volume":"135","author":"Adcock","year":"2022","journal-title":"Am. J. Med."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1198","DOI":"10.1056\/NEJMoa1403290","article-title":"Mepolizumab Treatment in Patients with Severe Eosinophilic Asthma","volume":"371","author":"Ortega","year":"2014","journal-title":"N. Engl. J. Med."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1779","DOI":"10.2147\/COPD.S162781","article-title":"Mepolizumab in the Treatment of Eosinophilic Chronic Obstructive Pulmonary Disease","volume":"14","author":"Mkorombindo","year":"2019","journal-title":"Int. J. Chronic Obstr. Pulm. Dis."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Bhatt, S.P., Rabe, K.F., Hanania, N.A., Vogelmeier, C.F., Cole, J., Bafadhel, M., Christenson, S.A., Papi, A., Singh, D., and Laws, E. (2023). Dupilumab for COPD with Type 2 Inflammation Indicated by Eosinophil Counts. N. Engl. J. Med.","DOI":"10.1056\/NEJMoa2303951"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"3279","DOI":"10.1172\/JCI85664","article-title":"Lung-Resident Eosinophils Represent a Distinct Regulatory Eosinophil Subset","volume":"126","author":"Mesnil","year":"2016","journal-title":"J. Clin. Investig."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/j.alit.2020.11.001","article-title":"The Multiple Functions and Subpopulations of Eosinophils in Tissues under Steady-State and Pathological Conditions","volume":"70","author":"Kanda","year":"2021","journal-title":"Allergol. Int."},{"key":"ref_19","unstructured":"(2020, September 14). Global Strategy for the Diagnosis, Management and Prevention of COPD, Global Initiative for Chronic Obstructive Lung Disease (GOLD). Available online: http:\/\/goldcopd.org\/."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1324","DOI":"10.1183\/09031936.00080312","article-title":"Multi-Ethnic Reference Values for Spirometry for the 3\u201395-Yr Age Range: The Global Lung Function 2012 Equations","volume":"40","author":"Quanjer","year":"2012","journal-title":"Eur. Respir. J."},{"key":"ref_21","unstructured":"GINA. Global Initiative for Asthma (GINA) (2020, September 14). Global Strategy for Asthma Management and Prevention. Global Initiative for Asthma\u2014Global Initiative for Asthma\u2014GINA. Available online: ginasthma.org."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"e70","DOI":"10.1164\/rccm.201908-1590ST","article-title":"Standardization of Spirometry 2019 Update. An Official American Thoracic Society and European Respiratory Society Technical Statement","volume":"200","author":"Graham","year":"2019","journal-title":"Am. J. Respir. Crit. Care Med."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1s","DOI":"10.1183\/09031936.02.00000102","article-title":"Standardised Methodology of Sputum Induction and Processing","volume":"20","author":"Sterk","year":"2002","journal-title":"Eur. Respir. J."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1080\/15412555.2016.1260539","article-title":"Eosinophilic and Neutrophilic Airway Inflammation in the Phenotyping of Mild-to-Moderate Asthma and Chronic Obstructive Pulmonary Disease","volume":"14","author":"Goryca","year":"2017","journal-title":"J. Chronic Obstr. Pulm. Dis."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"204","DOI":"10.1111\/cei.12676","article-title":"Similar Activation State of Neutrophils in Sputum of Asthma Patients Irrespective of Sputum Eosinophilia","volume":"182","author":"Tak","year":"2015","journal-title":"Clin. Exp. Immunol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"2763","DOI":"10.3389\/fimmu.2018.02763","article-title":"Eosinophil Extracellular Traps and Inflammatory Pathologies\u2014Untangling the Web!","volume":"9","author":"Mukherjee","year":"2018","journal-title":"Front. Immunol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1111\/j.1365-2249.2008.03729.x","article-title":"CD8 Chemokine Receptors in Chronic Obstructive Pulmonary Disease","volume":"154","author":"Smyth","year":"2008","journal-title":"Clin. Exp. Immunol."},{"key":"ref_28","first-page":"17","article-title":"Eotaxin and CCR3 Are up-Regulated in Exacerbations of Chronic Bronchitis","volume":"57","author":"Bocchino","year":"2002","journal-title":"Allergy"},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Strzelak, A., Ratajczak, A., Adamiec, A., and Feleszko, W. (2018). Tobacco Smoke Induces and Alters Immune Responses in the Lung Triggering Inflammation, Allergy, Asthma and Other Lung Diseases: A Mechanistic Review. Int. J. Environ. Res. Public Health, 15.","DOI":"10.3390\/ijerph15051033"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1186\/1465-9921-9-42","article-title":"Cigarette Smoke Enhances Th-2 Driven Airway Inflammation and Delays Inhalational Tolerance","volume":"9","author":"Moerloose","year":"2008","journal-title":"Respir. Res."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1080\/10937400802545094","article-title":"Cigarette Smoke, Inflammation, and Lung Injury: A Mechanistic Perspective","volume":"12","author":"Bhalla","year":"2009","journal-title":"J. Toxicol. Environ. Health Part B Crit. Rev."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"685","DOI":"10.1183\/09031936.01.00098501","article-title":"Increased Detection of Interleukin-;5 in Sputum by Addition of Protease Inhibitors","volume":"18","author":"Kelly","year":"2001","journal-title":"Eur. Respir. J."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"105875","DOI":"10.1016\/j.rmed.2020.105875","article-title":"Elevated Eosinophils, IL5 and IL8 in Induced Sputum in Asthma Patients with Accelerated FEV1 Decline","volume":"162","author":"Camlek","year":"2020","journal-title":"Respir. Med."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"904","DOI":"10.1016\/j.jaci.2015.02.039","article-title":"Correlation between CCL26 Production by Human Bronchial Epithelial Cells and Airway Eosinophils: Involvement in Patients with Severe Eosinophilic Asthma","volume":"136","author":"Larose","year":"2015","journal-title":"J. Allergy Clin. Immunol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"2517","DOI":"10.2147\/COPD.S222486","article-title":"Blood Neutrophils in COPD but Not Asthma Exhibit a Primed Phenotype with Downregulated CD62L Expression","volume":"14","author":"Lokwani","year":"2019","journal-title":"Int. J. Chronic Obstr. Pulm. Dis."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"606","DOI":"10.1046\/j.1365-2222.1998.00279.x","article-title":"CD11b and L-Selectin Expression on Eosinophils and Neutrophils in Blood and Induced Sputum of Patients with Asthma Compared with Normal Subjects","volume":"28","author":"Veen","year":"1998","journal-title":"Clin. Exp. Allergy"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"480","DOI":"10.1159\/000509715","article-title":"Increased CD11b and Decreased CD62L in Blood and Airway Neutrophils from Long-Term Smokers with and without COPD","volume":"12","author":"Stockfelt","year":"2020","journal-title":"J. Innate Immun."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"e52","DOI":"10.3324\/haematol.2019.219048","article-title":"Characterization of the Phenotype of Human Eosinophils and Their Progenitors in the Bone Marrow of Healthy Individuals","volume":"105","author":"Hassani","year":"2020","journal-title":"Haematologica"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"8454","DOI":"10.4049\/jimmunol.179.12.8454","article-title":"CD66b Regulates Adhesion and Activation of Human Eosinophils","volume":"179","author":"Yoon","year":"2007","journal-title":"J. Immunol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"791","DOI":"10.1189\/jlb.0911483","article-title":"CD66b Overexpression and Homotypic Aggregation of Human Peripheral Blood Neutrophils after Activation by a Gram-Positive Stimulus","volume":"91","author":"Schmidt","year":"2012","journal-title":"J. Leukoc. Biol."},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Curto, E., Mateus-Medina, \u00c9.F., Crespo-Lessmann, A., Osuna-G\u00f3mez, R., Ujald\u00f3n-Mir\u00f3, C., Garc\u00eda-Moral, A., Galv\u00e1n-Blasco, P., Soto-Retes, L., Ramos-Barb\u00f3n, D., and Plaza, V. (2022). Identification of Two Eosinophil Subsets in Induced Sputum from Patients with Allergic Asthma According to CD15 and CD66b Expression. Int. J. Environ. Res. Public Health, 19.","DOI":"10.3390\/ijerph192013400"},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Barnig, C., Alsaleh, G., Jung, N., Demb\u00e9l\u00e9, D., Paul, N., Poirot, A., Uring-Lambert, B., Georgel, P., de Blay, F., and Bahram, S. (2015). Circulating Human Eosinophils Share a Similar Transcriptional Profile in Asthma and Other Hypereosinophilic Disorders. PLoS ONE, 10.","DOI":"10.1371\/journal.pone.0141740"}],"container-title":["Cells"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2073-4409\/12\/12\/1631\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T19:55:14Z","timestamp":1760126114000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2073-4409\/12\/12\/1631"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,6,15]]},"references-count":42,"journal-issue":{"issue":"12","published-online":{"date-parts":[[2023,6]]}},"alternative-id":["cells12121631"],"URL":"https:\/\/doi.org\/10.3390\/cells12121631","relation":{},"ISSN":["2073-4409"],"issn-type":[{"value":"2073-4409","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,6,15]]}}}