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In this study, we isolated and cultured primary retinal pigment epithelium (RPE) cells from marmosets to investigate the mechanisms underlying RPE dysfunction in aging and age-related macular degeneration (AMD). We confirmed that our culture conditions and materials supported the formation of RPE monolayers with functional tight junctions that closely resembled the in vivo RPE. Since serum has been shown to induce epithelial\u2013mesenchymal transition (EMT) in RPE cells, we compared the effects of fetal bovine serum (FBS) with serum-free supplements B27 on transepithelial electrical resistance (TER), cell proliferation, and morphological characteristics. Additionally, we assessed the age-related morphological changes of in vivo and primary RPE cells. Our results indicate that primary marmoset RPE cells exhibit in vivo-like characteristics, while cells obtained from an older donor show evidence of aging, including a failure to form a polarized monolayer, low TER, and delayed cell cycle. In conclusion, our primary marmoset RPE cells provide a reliable in vitro model for developing novel therapeutics for visual-threatening disorders such as AMD, which can be used before animal experiments using marmosets.<\/jats:p>","DOI":"10.3390\/cells12121644","type":"journal-article","created":{"date-parts":[[2023,6,16]],"date-time":"2023-06-16T02:54:33Z","timestamp":1686884073000},"page":"1644","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Isolation and Characterization of the Primary Marmoset (Callithrix jacchus) Retinal Pigment Epithelial Cells"],"prefix":"10.3390","volume":"12","author":[{"given":"Ha Young","family":"Jang","sequence":"first","affiliation":[{"name":"Fight Against Angiogenesis-Related Blindness (FARB) Laboratory, Clinical Research Institute, Seoul National University Hospital, Seoul 03082, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0812-1515","authenticated-orcid":false,"given":"Chang Sik","family":"Cho","sequence":"additional","affiliation":[{"name":"Fight Against Angiogenesis-Related Blindness (FARB) Laboratory, Clinical Research Institute, Seoul National University Hospital, Seoul 03082, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Young Mi","family":"Shin","sequence":"additional","affiliation":[{"name":"Fight Against Angiogenesis-Related Blindness (FARB) Laboratory, Clinical Research Institute, Seoul National University Hospital, Seoul 03082, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jina","family":"Kwak","sequence":"additional","affiliation":[{"name":"Graduate School of Translational Medicine, Seoul National University College of Medicine, Seoul 03080, Republic of Korea"},{"name":"Department of Experimental Animal Research, Biomedical Research Institute, Seoul National University Hospital, Seoul 03080, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Young Hoon","family":"Sung","sequence":"additional","affiliation":[{"name":"Asan Institute for Life Sciences, Asan Medical Center, Seoul 05505, Republic of Korea"},{"name":"Department of Convergence Medicine, University of Ulsan College of Medicine, Seoul 05505, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Byeong-Cheol","family":"Kang","sequence":"additional","affiliation":[{"name":"Graduate School of Translational Medicine, Seoul National University College of Medicine, Seoul 03080, Republic of Korea"},{"name":"Department of Experimental Animal Research, Biomedical Research Institute, Seoul National University Hospital, Seoul 03080, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeong Hun","family":"Kim","sequence":"additional","affiliation":[{"name":"Fight Against Angiogenesis-Related Blindness (FARB) Laboratory, Clinical Research Institute, Seoul National University Hospital, Seoul 03082, Republic of Korea"},{"name":"Department of Biomedical Sciences & Ophthalmology, Seoul National University College of Medicine, Seoul 03080, Republic of Korea"},{"name":"Institute of Reproductive Medicine and Population, Seoul National University College of Medicine, Seoul 03080, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,6,16]]},"reference":[{"key":"ref_1","first-page":"57","article-title":"The retinal pigment epithelium and interphotoreceptor matrix: Structure and function","volume":"Volume 1","author":"Ogden","year":"1994","journal-title":"Retina: Basic Science and Inherited Retinal Disease"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Naylor, A., Hopkins, A., Hudson, N., and Campbell, M. (2019). Tight junctions of the outer blood retina barrier. Int. J. Mol. Sci., 21.","DOI":"10.3390\/ijms21010211"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"727870","DOI":"10.3389\/fphar.2021.727870","article-title":"Function and diseases of the retinal pigment epithelium","volume":"12","author":"Yang","year":"2021","journal-title":"Front. Pharmacol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1038\/s41572-021-00265-2","article-title":"Age-related macular degeneration","volume":"7","author":"Fleckenstein","year":"2021","journal-title":"Nat. Rev. Dis. Prim."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"487","DOI":"10.1016\/j.mam.2012.06.003","article-title":"Animal models of age related macular degeneration","volume":"33","author":"Pennesi","year":"2012","journal-title":"Mol. Asp. Med."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"421","DOI":"10.1242\/dmm.017236","article-title":"Cellular models and therapies for age-related macular degeneration","volume":"8","author":"Forest","year":"2015","journal-title":"Dis. Models Mech."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"788","DOI":"10.1167\/iovs.07-0777","article-title":"A novel serum-free method for culturing human prenatal retinal pigment epithelial cells","volume":"49","author":"Gamm","year":"2008","journal-title":"Investig. Ophthalmol. Vis. Sci."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"109046","DOI":"10.1016\/j.exer.2022.109046","article-title":"Reassessing the suitability of APRE-19 cells as a valid model of native RPE biology","volume":"219","author":"Pfeffer","year":"2022","journal-title":"Exp. Eye Res."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"3612","DOI":"10.1167\/iovs.05-1622","article-title":"Confluent monolayers of cultured human fetal retinal pigment epithelium exhibit morphology and physiology of native tissue","volume":"47","author":"Maminishkis","year":"2006","journal-title":"Investig. Ophthalmol. Vis. Sci."},{"key":"ref_10","first-page":"56997","article-title":"Primary cell cultures from the mouse retinal pigment epithelium","volume":"133","author":"Shang","year":"2018","journal-title":"J. Vis. Exp."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"2390","DOI":"10.1016\/j.neurobiolaging.2011.12.009","article-title":"Lipofuscin can be eliminated from the retinal pigment epithelium of monkeys","volume":"33","author":"Julien","year":"2012","journal-title":"Neurobiol. 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Methods"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1016\/j.neures.2015.01.008","article-title":"The marmoset monkey as a model for visual neuroscience","volume":"93","author":"Mitchell","year":"2015","journal-title":"Neurosci. Res."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1016\/0278-4327(91)90015-T","article-title":"Improved methodology for cell culture of human and monkey retinal pigment epithelium","volume":"10","author":"Pfeffer","year":"1991","journal-title":"Prog. Retin. Res."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"2041731416650838","DOI":"10.1177\/2041731416650838","article-title":"Methods for culturing retinal pigment epithelial cells: A review of current protocols and future recommendations","volume":"7","author":"Fronk","year":"2016","journal-title":"J. Tissue Eng."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"662","DOI":"10.1038\/nprot.2009.33","article-title":"A protocol for the culture and differentiation of highly polarized human retinal pigment epithelial cells","volume":"4","author":"Sonoda","year":"2009","journal-title":"Nat. Protoc."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1206","DOI":"10.1038\/nprot.2016.065","article-title":"Isolation, culture and characterization of primary mouse RPE cells","volume":"11","author":"Garland","year":"2016","journal-title":"Nat. Protoc."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"74","DOI":"10.1016\/j.exer.2014.05.003","article-title":"A detailed three-step protocol for live imaging of intracellular traffic in polarized primary porcine RPE monolayers","volume":"124","author":"Toops","year":"2014","journal-title":"Exp. Eye Res."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"523","DOI":"10.1038\/nature08090","article-title":"Generation of transgenic non-human primates with germline transmission","volume":"459","author":"Sasaki","year":"2009","journal-title":"Nature"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1167\/iovs.62.2.32","article-title":"Morphometric analysis of retinal pigment epithelial cells from C57BL\/6J mice during aging","volume":"62","author":"Kim","year":"2021","journal-title":"Investig. Ophthalmol. Vis. Sci."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"436","DOI":"10.1111\/acel.12447","article-title":"Retinal pigment epithelial cell multinucleation in the agining eye\u2014A mechanism to repair damage and maintain homoeostasis","volume":"15","author":"Chen","year":"2016","journal-title":"Aging Cell"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"109170","DOI":"10.1016\/j.exer.2022.109170","article-title":"Cell culture models to study retinal pigment epithelium-related pathogenesis in age-related macular degeneration","volume":"222","author":"Bharti","year":"2022","journal-title":"Exp. Eye Res."},{"key":"ref_25","first-page":"60","article-title":"Appropriately differentiated ARPE-19 cells regain phenotype and gene expression profiles similar to those of native RPE cells","volume":"23","author":"Samuel","year":"2017","journal-title":"Mol. Vis."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"417865","DOI":"10.1155\/2012\/417865","article-title":"Derivation of neural progenitors and retinal pigment epithelium from common marmoset and human pluripotent stem cells","volume":"2012","author":"Torrez","year":"2012","journal-title":"Stem Cells Int."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"160","DOI":"10.1016\/j.exer.2018.08.005","article-title":"A cell culture condition that induces the mesenchymal-epithelial transition of dedifferentiated porcine retinal pigment epithelial cells","volume":"177","author":"Tian","year":"2018","journal-title":"Exp. Eye Res."},{"key":"ref_28","first-page":"706","article-title":"The roles of signaling pathways in epithelial-to-mesenchymal transition of PVR","volume":"21","author":"Chen","year":"2015","journal-title":"Mol. Vis."},{"key":"ref_29","first-page":"14","article-title":"A cell culture medium that supports the differentiation of human retinal pigment epithelium into functionally polarized monolayers","volume":"7","author":"Hu","year":"2001","journal-title":"Mol. Vis."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"807","DOI":"10.1006\/exer.1998.0492","article-title":"Serum-free media for culturing and serial-passaging of adult human retinal pigment epithelium","volume":"66","author":"Tezel","year":"1998","journal-title":"Exp. Eye Res."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"2599","DOI":"10.1021\/pr060121j","article-title":"Secreted proteome profiling in human RPe cell cultures derived from donors with age related macular degeneration and age matched healthy donors","volume":"5","author":"An","year":"2006","journal-title":"J. Proteome Res."},{"key":"ref_32","first-page":"989","article-title":"The retinal pigment epithelium undergoes massive apoptosis during early differentiation and pigmentation of the optic cup","volume":"17","author":"Pequignot","year":"2011","journal-title":"Mol. Vis."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1016\/j.jphotobiol.2014.07.016","article-title":"Docosahexaenoic acid aggravates photooxidative damage in retinal pigment epithelial cells via lipid peroxidation","volume":"140","author":"Liu","year":"2014","journal-title":"J. Photochem. Photobiol. B"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1016\/j.exer.2013.12.018","article-title":"Barrier properties of cultured retinal pigment epithelium","volume":"126","author":"Rizzolo","year":"2014","journal-title":"Exp. Eye Res."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"3930","DOI":"10.1091\/mbc.e09-04-0320","article-title":"Zo-1 stanilizes the tight junction solute barrier through coupling to the perijunctional cytoskeleton","volume":"20","author":"Fanning","year":"2009","journal-title":"Mol. Biol. Cell"},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Georgiadis, A., Marion Tschernutter, M., Bainbridge, J.W.B., Balaggan, K.S., Mowat, F., West, E.L., Munro, P.M.G., Thrasher, A.J., Matter, K., and Balda, M.S. (2010). The tight junction associated signalling proteins ZO-1 and ZONAB regulate retinal pigment epithelium homeostasis in mice. PLoS ONE, 5.","DOI":"10.1371\/journal.pone.0015730"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1075","DOI":"10.1016\/j.stemcr.2018.01.017","article-title":"Quantified f-actin morphology is predictive of phagocytic capacity of stem cell-derived retinal pigment epithelium","volume":"10","author":"Charniga","year":"2018","journal-title":"Stem Cell Rep."}],"container-title":["Cells"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2073-4409\/12\/12\/1644\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T19:56:15Z","timestamp":1760126175000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2073-4409\/12\/12\/1644"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,6,16]]},"references-count":37,"journal-issue":{"issue":"12","published-online":{"date-parts":[[2023,6]]}},"alternative-id":["cells12121644"],"URL":"https:\/\/doi.org\/10.3390\/cells12121644","relation":{},"ISSN":["2073-4409"],"issn-type":[{"value":"2073-4409","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,6,16]]}}}